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The map label for this gene is nodE [H]

Identifier: 99081062

GI number: 99081062

Start: 1311374

End: 1312582

Strand: Direct

Name: nodE [H]

Synonym: TM1040_1221

Alternate gene names: 99081062

Gene position: 1311374-1312582 (Clockwise)

Preceding gene: 99081061

Following gene: 99081064

Centisome position: 40.97

GC content: 59.97

Gene sequence:

>1209_bases
ATGAAACGGGTTGTGATTACCGGCGCCGGGACCATCAACGCGCTGGGGCATTCGGTCGCCGAAACCTTGGAGTCCATGCG
TGAAGGGCGTTGCGGAATTGGCGAGTTGGACTTTCGCGATGTTGATCGTCTTGCGATTAAGATAGGCGGGCAAGTCCAGA
ATTTCGAAGCAGAAGGTCGCTTCAATCGCCAGCAGATGGTGCTCTACGATCGGTTCACGCAGTTCACCTTGGTGGCGGCC
TCTGAGGCGATCCGTCAATCCGGTCTCGAGTTCACCGGCGACCTGGCTGCGCGGTCCGGGGTCGTGCTGGGAACGGCAGG
CGGTGGTGTCTCGACCTGGGATGACAACTACCGATCGGTCTACGAGGATGGCAAGAATCGCGTGCATCCTTTTGTTGTTC
CGAAACTTATGAACAACGCCGCTGCCAGCCATGTGTCGATGGAATACAACCTCAAGGGGCCGAGCTTTACGGTTTCTACC
GCCTGTGCGTCCTCAAATCATGCGATGGCGCAGGCCTTTCAGATGGTGCGCAGCGGCATGAGTCCCGCCATGGTCACGGG
TGGGTCTGAATCGATGCTCTGCTTCGGCGGGGTGAAGGCGTGGGAAGGACTGCGCGTGATGTCCAAGGACGCCTGCCGCC
CCTTCAGTGCCAACCGCAATGGCATGGTGCAGGGCGAGGGTGCTGGGATCTTTGTGTTTGAGGAATATGAACACGCCAAG
GCCCGCGGCGCGGAGATCCTGTGCGAAGTGATCGGCTACGCCATGAGCTCGGATGCGGCCGACATTGTGATGCCCAGCAA
ACAGGGCGCGGCGCGTGCAATCTCGGGCGCGCTCAGGGATGCTCAGATCAACGCCAGCGACGTGGGGTATATCAACGCGC
ATGGGACCGGCACCGCCGCCAATGACAAGACCGAATGCGCAGCTGTTGCGGATGTCTTTGGGCCGCATGCTGATAACCTC
ATGATTTCGTCAACAAAATCCATGCACGGTCACCTTATTGGTGGGACGGGCGCGGTGGAGCTTTTGGCCTGTATCATGGC
GTTGCGCGACGGGGTGATCGCGCCGACCATCGGCTATGAAGAGGCCGACCCTGAATGCGCGCTGGACGTGGTGCCCAATG
AGGCGCGAGAGGCCAAGGTAGACGTGGCCTTGTCAAATGCTTTTGCCTTTGGCGGCCTCAACGCTGTGCTAGCGCTGCGT
CGGGTCTGA

Upstream 100 bases:

>100_bases
TTCGATATTTCAAATGTCGCAGCCATTGTGACCGGGATTGACAAGCTGATCGCTGAAAAGGGCTAACCGCCCAGAGCGCC
ATCGGACAAGAGGATTTGCG

Downstream 100 bases:

>100_bases
CGGTCAGGGCTGAGACCTGTCCGAGCGCACAGTAATCTTCTGCCGGAGCTCAAACCCGCCAGACCTCATATCGACCAGAC
ATTAAAAAGCCCCGCCTGAT

Product: 3-oxoacyl-[acyl-carrier-protein] synthase II

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRVVITGAGTINALGHSVAETLESMREGRCGIGELDFRDVDRLAIKIGGQVQNFEAEGRFNRQQMVLYDRFTQFTLVAA
SEAIRQSGLEFTGDLAARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEYNLKGPSFTVST
ACASSNHAMAQAFQMVRSGMSPAMVTGGSESMLCFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAGIFVFEEYEHAK
ARGAEILCEVIGYAMSSDAADIVMPSKQGAARAISGALRDAQINASDVGYINAHGTGTAANDKTECAAVADVFGPHADNL
MISSTKSMHGHLIGGTGAVELLACIMALRDGVIAPTIGYEEADPECALDVVPNEAREAKVDVALSNAFAFGGLNAVLALR
RV

Sequences:

>Translated_402_residues
MKRVVITGAGTINALGHSVAETLESMREGRCGIGELDFRDVDRLAIKIGGQVQNFEAEGRFNRQQMVLYDRFTQFTLVAA
SEAIRQSGLEFTGDLAARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEYNLKGPSFTVST
ACASSNHAMAQAFQMVRSGMSPAMVTGGSESMLCFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAGIFVFEEYEHAK
ARGAEILCEVIGYAMSSDAADIVMPSKQGAARAISGALRDAQINASDVGYINAHGTGTAANDKTECAAVADVFGPHADNL
MISSTKSMHGHLIGGTGAVELLACIMALRDGVIAPTIGYEEADPECALDVVPNEAREAKVDVALSNAFAFGGLNAVLALR
RV
>Mature_402_residues
MKRVVITGAGTINALGHSVAETLESMREGRCGIGELDFRDVDRLAIKIGGQVQNFEAEGRFNRQQMVLYDRFTQFTLVAA
SEAIRQSGLEFTGDLAARSGVVLGTAGGGVSTWDDNYRSVYEDGKNRVHPFVVPKLMNNAAASHVSMEYNLKGPSFTVST
ACASSNHAMAQAFQMVRSGMSPAMVTGGSESMLCFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAGIFVFEEYEHAK
ARGAEILCEVIGYAMSSDAADIVMPSKQGAARAISGALRDAQINASDVGYINAHGTGTAANDKTECAAVADVFGPHADNL
MISSTKSMHGHLIGGTGAVELLACIMALRDGVIAPTIGYEEADPECALDVVPNEAREAKVDVALSNAFAFGGLNAVLALR
RV

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=416, Percent_Identity=36.0576923076923, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI223972627, Length=162, Percent_Identity=36.4197530864198, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI41872631, Length=272, Percent_Identity=27.2058823529412, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787337, Length=410, Percent_Identity=40.7317073170732, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1788663, Length=415, Percent_Identity=32.289156626506, Blast_Score=200, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71983541, Length=413, Percent_Identity=32.6876513317191, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI71983535, Length=262, Percent_Identity=34.3511450381679, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI212642053, Length=360, Percent_Identity=25.2777777777778, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17550940, Length=232, Percent_Identity=25.8620689655172, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320904, Length=444, Percent_Identity=34.009009009009, Blast_Score=219, Evalue=9e-58,
Organism=Drosophila melanogaster, GI24644341, Length=417, Percent_Identity=34.5323741007194, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI221330659, Length=370, Percent_Identity=26.4864864864865, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19920632, Length=370, Percent_Identity=26.4864864864865, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581345, Length=352, Percent_Identity=25, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 42419; Mature: 42419

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00098 THIOLASE_1 ; PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVVITGAGTINALGHSVAETLESMREGRCGIGELDFRDVDRLAIKIGGQVQNFEAEGR
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEECCEEECCCCCCC
FNRQQMVLYDRFTQFTLVAASEAIRQSGLEFTGDLAARSGVVLGTAGGGVSTWDDNYRSV
CCCCEEHEEHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCEEEEECCCCCCCCCCHHHHH
YEDGKNRVHPFVVPKLMNNAAASHVSMEYNLKGPSFTVSTACASSNHAMAQAFQMVRSGM
HHCCCCCCCCEEHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCC
SPAMVTGGSESMLCFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAGIFVFEEYEHAK
CCEEEECCCCCEEEECCCHHHCCCHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCHHHH
ARGAEILCEVIGYAMSSDAADIVMPSKQGAARAISGALRDAQINASDVGYINAHGTGTAA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
NDKTECAAVADVFGPHADNLMISSTKSMHGHLIGGTGAVELLACIMALRDGVIAPTIGYE
CCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCC
EADPECALDVVPNEAREAKVDVALSNAFAFGGLNAVLALRRV
CCCCCEEEEECCCCCHHCEEEEEEECHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MKRVVITGAGTINALGHSVAETLESMREGRCGIGELDFRDVDRLAIKIGGQVQNFEAEGR
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEECCEEECCCCCCC
FNRQQMVLYDRFTQFTLVAASEAIRQSGLEFTGDLAARSGVVLGTAGGGVSTWDDNYRSV
CCCCEEHEEHHHHHHHHHHHHHHHHHCCCCEECCHHHCCCEEEEECCCCCCCCCCHHHHH
YEDGKNRVHPFVVPKLMNNAAASHVSMEYNLKGPSFTVSTACASSNHAMAQAFQMVRSGM
HHCCCCCCCCEEHHHHHHHHHHHHEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCC
SPAMVTGGSESMLCFGGVKAWEGLRVMSKDACRPFSANRNGMVQGEGAGIFVFEEYEHAK
CCEEEECCCCCEEEECCCHHHCCCHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCHHHH
ARGAEILCEVIGYAMSSDAADIVMPSKQGAARAISGALRDAQINASDVGYINAHGTGTAA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCC
NDKTECAAVADVFGPHADNLMISSTKSMHGHLIGGTGAVELLACIMALRDGVIAPTIGYE
CCCHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHCCCEECCCCCC
EADPECALDVVPNEAREAKVDVALSNAFAFGGLNAVLALRRV
CCCCCEEEEECCCCCHHCEEEEEEECHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]