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The map label for this gene is gltX

Identifier: 99080974

GI number: 99080974

Start: 1218376

End: 1219776

Strand: Reverse

Name: gltX

Synonym: TM1040_1133

Alternate gene names: 99080974

Gene position: 1219776-1218376 (Counterclockwise)

Preceding gene: 99080976

Following gene: 99080973

Centisome position: 38.11

GC content: 59.96

Gene sequence:

>1401_bases
ATGTCCCAACCGGTCGTCACCCGCTTCGCCCCTTCGCCCACAGGCTTTTTGCACATCGGCGGCGCGCGCACCGCGCTGTT
TAACTGGCTTTTTGCGCGTGGCCGCGGCGGCAAATTCCTGCTTCGGATCGAAGACACCGACCGCGCCCGCTCCACCCCGG
AAGCCACCGCCGCCATCCTGCAGGGCATGGCGTGGCTTGGGCTGGATCACGACGGCGAGGTGGTAAGCCAGTTTGAAGGC
GCCGAGCGCCATGCCGAAGTGGCGCACCAGCTGCTCGCCGAGGGCAAAGCCTACAAGTGTTTCTCCACCCAGGAAGAAAT
CGCCGCCTTTCGCGAGGCCGCCAAGGCCGAGGGCAAATCGACCCTGTTTCACTCGCCCTGGCGCGACACGCCCGAGTCTG
AGCACCCCGATGCGCCCTTTGCGATCCGCATCAAGGCGCCCAAAGACGGCGAAACCGTCATTCGCGATCAGGTTCAGGGC
GATGTGACCATCAAAAATGCGCAATTGGACGATATGATCCTGCTGCGCTCGGATGGCACGCCGGTCTATATGCTGGCCGT
GGTGGTGGACGATCACGATATGGGTGTGACCCATGTGATCCGGGGCGACGATCACCTGATGAATGCAGCGCGCCAGATGA
TGATCTACGAGGCGATGGGCTGGGACGTGCCGGTCTATGCTCATATCCCGCTCATTCACGGCTCGGACGGCAAGAAGCTC
TCCAAACGCCATGGCGCGCTTGGGGCGCAGGAATATCAGGCGATGGGCTATCCCGCGGCAGGCATGCGCAACTATCTTGC
GCGTCTGGGCTGGTCGCATGGCGATGACGAGTTCTTTACCGATGAGCAGGCCAAAGACTGGTTCGATCTGGACGGAATCG
GCAAAAGTCCGGCCCGATTTGACACCAAAAAGCTCGAAAATCTCTGTGGCCAACATATCGCAATCGCGGATGATGCTGCA
CTGCGGCAAGAAATCGAGGCCTATCTGGACGCGGCCGGTCAGCCGGCCCTAAATGACGTACAGTCACAAGGGCTTGAGCG
GGCAATGTACTGCCTCAAGGATCGGGCAAAAACCTTCCCCGAACTCCTTGAAAAAGCGCATTTTGTGCTGACCTCACGCC
CCCTGGAACGGGATGCGAAGGCCGAAAAAGCACTCGACCCAGTATCCATTGGTATACTGAACGAATTGACGCCGCATCTG
CAAAATGCTAGCTGGTCGCGAGACGAGTTAGAGGCCGCCCTGAATGAATTTGCCGCGGCTAAGGACACGAAGTTTGGCAA
ATTGGCTGGCCCCCTGCGGGCTGTGCTTGCAGGCCGCGCGGTAACGCCTTCGGTCTTTGACATGATGATTTGCTTGGGCC
GCGAGGAAACTCTGGCCCGGCTCAAGGACGTAACTGGCTGA

Upstream 100 bases:

>100_bases
AAGAGCGCCAAACACGCGCACGCTTGCTCCCTGCCTTCTGGCTCGGTACACAGGCGACAAACGTTGACCCCACAAGGTTT
CCAAAAGGTAAATGCCCCAT

Downstream 100 bases:

>100_bases
GCACCCGTCAGGATCTCTTGTTAAGGTCTGGGTAAGCCCGTTCGTATAGGACTGCGTCTGCGCCTTTGATGGCGCGGGCG
CCCCGCTGCGCGGGACGGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSQPVVTRFAPSPTGFLHIGGARTALFNWLFARGRGGKFLLRIEDTDRARSTPEATAAILQGMAWLGLDHDGEVVSQFEG
AERHAEVAHQLLAEGKAYKCFSTQEEIAAFREAAKAEGKSTLFHSPWRDTPESEHPDAPFAIRIKAPKDGETVIRDQVQG
DVTIKNAQLDDMILLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLMNAARQMMIYEAMGWDVPVYAHIPLIHGSDGKKL
SKRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDEQAKDWFDLDGIGKSPARFDTKKLENLCGQHIAIADDAA
LRQEIEAYLDAAGQPALNDVQSQGLERAMYCLKDRAKTFPELLEKAHFVLTSRPLERDAKAEKALDPVSIGILNELTPHL
QNASWSRDELEAALNEFAAAKDTKFGKLAGPLRAVLAGRAVTPSVFDMMICLGREETLARLKDVTG

Sequences:

>Translated_466_residues
MSQPVVTRFAPSPTGFLHIGGARTALFNWLFARGRGGKFLLRIEDTDRARSTPEATAAILQGMAWLGLDHDGEVVSQFEG
AERHAEVAHQLLAEGKAYKCFSTQEEIAAFREAAKAEGKSTLFHSPWRDTPESEHPDAPFAIRIKAPKDGETVIRDQVQG
DVTIKNAQLDDMILLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLMNAARQMMIYEAMGWDVPVYAHIPLIHGSDGKKL
SKRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDEQAKDWFDLDGIGKSPARFDTKKLENLCGQHIAIADDAA
LRQEIEAYLDAAGQPALNDVQSQGLERAMYCLKDRAKTFPELLEKAHFVLTSRPLERDAKAEKALDPVSIGILNELTPHL
QNASWSRDELEAALNEFAAAKDTKFGKLAGPLRAVLAGRAVTPSVFDMMICLGREETLARLKDVTG
>Mature_465_residues
SQPVVTRFAPSPTGFLHIGGARTALFNWLFARGRGGKFLLRIEDTDRARSTPEATAAILQGMAWLGLDHDGEVVSQFEGA
ERHAEVAHQLLAEGKAYKCFSTQEEIAAFREAAKAEGKSTLFHSPWRDTPESEHPDAPFAIRIKAPKDGETVIRDQVQGD
VTIKNAQLDDMILLRSDGTPVYMLAVVVDDHDMGVTHVIRGDDHLMNAARQMMIYEAMGWDVPVYAHIPLIHGSDGKKLS
KRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDEQAKDWFDLDGIGKSPARFDTKKLENLCGQHIAIADDAAL
RQEIEAYLDAAGQPALNDVQSQGLERAMYCLKDRAKTFPELLEKAHFVLTSRPLERDAKAEKALDPVSIGILNELTPHLQ
NASWSRDELEAALNEFAAAKDTKFGKLAGPLRAVLAGRAVTPSVFDMMICLGREETLARLKDVTG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=29.4949494949495, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI4826960, Length=293, Percent_Identity=24.9146757679181, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788743, Length=460, Percent_Identity=39.5652173913043, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=36.7768595041322, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1786895, Length=208, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=29.3279022403259, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=322, Percent_Identity=37.2670807453416, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321192, Length=212, Percent_Identity=31.1320754716981, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=181, Percent_Identity=30.939226519337, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=487, Percent_Identity=30.8008213552361, Blast_Score=225, Evalue=6e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_SILST (Q1GHK0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613128.1
- ProteinModelPortal:   Q1GHK0
- SMR:   Q1GHK0
- STRING:   Q1GHK0
- GeneID:   4077254
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1133
- NMPDR:   fig|292414.1.peg.3732
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q1GHK0
- ProtClustDB:   PRK01406
- BioCyc:   SSP292414:TM1040_1133-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51360; Mature: 51229

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPVVTRFAPSPTGFLHIGGARTALFNWLFARGRGGKFLLRIEDTDRARSTPEATAAIL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
QGMAWLGLDHDGEVVSQFEGAERHAEVAHQLLAEGKAYKCFSTQEEIAAFREAAKAEGKS
HHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCC
TLFHSPWRDTPESEHPDAPFAIRIKAPKDGETVIRDQVQGDVTIKNAQLDDMILLRSDGT
CEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEECCCCCCEEEEECCCC
PVYMLAVVVDDHDMGVTHVIRGDDHLMNAARQMMIYEAMGWDVPVYAHIPLIHGSDGKKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHH
SKRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDEQAKDWFDLDGIGKSPARF
HHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
DTKKLENLCGQHIAIADDAALRQEIEAYLDAAGQPALNDVQSQGLERAMYCLKDRAKTFP
CHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELLEKAHFVLTSRPLERDAKAEKALDPVSIGILNELTPHLQNASWSRDELEAALNEFAAA
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KDTKFGKLAGPLRAVLAGRAVTPSVFDMMICLGREETLARLKDVTG
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SQPVVTRFAPSPTGFLHIGGARTALFNWLFARGRGGKFLLRIEDTDRARSTPEATAAIL
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHH
QGMAWLGLDHDGEVVSQFEGAERHAEVAHQLLAEGKAYKCFSTQEEIAAFREAAKAEGKS
HHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCC
TLFHSPWRDTPESEHPDAPFAIRIKAPKDGETVIRDQVQGDVTIKNAQLDDMILLRSDGT
CEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEECCCCCCEEEEECCCC
PVYMLAVVVDDHDMGVTHVIRGDDHLMNAARQMMIYEAMGWDVPVYAHIPLIHGSDGKKL
CEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCHH
SKRHGALGAQEYQAMGYPAAGMRNYLARLGWSHGDDEFFTDEQAKDWFDLDGIGKSPARF
HHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
DTKKLENLCGQHIAIADDAALRQEIEAYLDAAGQPALNDVQSQGLERAMYCLKDRAKTFP
CHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELLEKAHFVLTSRPLERDAKAEKALDPVSIGILNELTPHLQNASWSRDELEAALNEFAAA
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
KDTKFGKLAGPLRAVLAGRAVTPSVFDMMICLGREETLARLKDVTG
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA