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The map label for this gene is ccsA [H]

Identifier: 99080973

GI number: 99080973

Start: 1216941

End: 1218236

Strand: Reverse

Name: ccsA [H]

Synonym: TM1040_1132

Alternate gene names: 99080973

Gene position: 1218236-1216941 (Counterclockwise)

Preceding gene: 99080974

Following gene: 99080972

Centisome position: 38.06

GC content: 59.41

Gene sequence:

>1296_bases
ATGACCGACACACAGAAAACAGCCCAGCTCAGCTTGAATGGTGAGACCTACGAACTCCCCATCTTCTCGCCGACTGCCGG
GCCCGATGTTCTGGATATCCGCAAGCTCTACGCGCAGTCCGGAGTGTTCACCTATGATCCGGGCTTCACCTCCACTGCGA
GCTGTGACAGCACGATCACCTATATCGACGGCAACGAAGGTGTGCTGCTGCATCGTGGCTATCCGATCGACCAGCTGGCC
GGTAAATCCCACTACCTCGAAGTCTGCTACCTGCTCCTTTATGGTGAGCTGCCGACCGCGACGCAGCTTGAGGATTTCGA
GACCCGTGTGACCCGCCACACCATGGTCCACGAGCAGATGCACAACTTCTTCCGTGGTTTCCGCCGGGACGCGCACCCGA
TGGCAACCTTGGTGGGCGTTGTGGGCGCAATGTCGGCCTTCTATCACGACTCCACCGACATCTCCGATCCGTGGCAGCGT
GAAGTGGCTGCAATCCGCCTGATTGCAAAACTGCCGACGATTGCTGCGATGGCCTACAAGTATTCCATCGGCCAGCCGTT
TGTGTATCCGCGCAACGATCTGGATTACGCCGCGAACTTCCTGCACATGTGTTTCTCTGTCCCGGCCGAGAACTACACCG
TGGACCCGATCCTGAGCCGCGCGATGGACCGGATCTTCACCCTGCATGCGGACCACGAGCAGAACGCCTCGACCTCGACC
GTGCGTCTGGCGTCCTCCTCCGGGGCCAACCCCTTTGCCTGTATCGCAGCTGGCATTGCCTGCCTCTGGGGCCCTGCCCA
CGGTGGCGCCAACCAGGCCTGCCTCGAGATGCTCAAGGAAATCGGCTCTGTCGATCGCATTCCGGAGTTCATCGCACGCG
CCAAAGACAAGAACGATCCGTTCCGTCTGATGGGCTTTGGCCACCGCGTCTACAAGAACACGGACCCGCGCGCCAAGGTG
CTGAAGCAGTCTGCAGACGAAGTGCTGGAGCTTCTGGGCGTGGAAGACAACCCGCTCCTGCAGGTCGCCAAGGAACTCGA
GCAGACCGCGCTCAACGATCCGTATTTCATCGAGAAAAAGCTGTTCCCGAACGTGGACTTCTACTCGGGCATCATCCTCG
AGGCGATGGGCTTCCCGACCTCCATGTTCACCCCGATCTTTGCGCTGTCGCGCACCGTCGGCTGGATCTCCCAGTGGAAA
GAAATGATCGGTGATCCGCAGAACAAAATCGGCCGTCCGCGCCAATTGTATCTCGGTGAGACACAGCGCGACTATGTCGA
CATCGAAAACCGCTAA

Upstream 100 bases:

>100_bases
GTTCGTATAGGACTGCGTCTGCGCCTTTGATGGCGCGGGCGCCCCGCTGCGCGGGACGGGCCACCCGCCCTCTCTGCGCC
TGTAACAGGAAGGGACATCT

Downstream 100 bases:

>100_bases
GAGAAACTCTCTCTGAAATGCGTGCGCCGCCCCAATTCGGGGCGGCGTTTTCATTTAAAGCACTGATAATAAGTACAATA
TTTCCGGTCGAGGTCAGGTA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTDTQKTAQLSLNGETYELPIFSPTAGPDVLDIRKLYAQSGVFTYDPGFTSTASCDSTITYIDGNEGVLLHRGYPIDQLA
GKSHYLEVCYLLLYGELPTATQLEDFETRVTRHTMVHEQMHNFFRGFRRDAHPMATLVGVVGAMSAFYHDSTDISDPWQR
EVAAIRLIAKLPTIAAMAYKYSIGQPFVYPRNDLDYAANFLHMCFSVPAENYTVDPILSRAMDRIFTLHADHEQNASTST
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGSVDRIPEFIARAKDKNDPFRLMGFGHRVYKNTDPRAKV
LKQSADEVLELLGVEDNPLLQVAKELEQTALNDPYFIEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWK
EMIGDPQNKIGRPRQLYLGETQRDYVDIENR

Sequences:

>Translated_431_residues
MTDTQKTAQLSLNGETYELPIFSPTAGPDVLDIRKLYAQSGVFTYDPGFTSTASCDSTITYIDGNEGVLLHRGYPIDQLA
GKSHYLEVCYLLLYGELPTATQLEDFETRVTRHTMVHEQMHNFFRGFRRDAHPMATLVGVVGAMSAFYHDSTDISDPWQR
EVAAIRLIAKLPTIAAMAYKYSIGQPFVYPRNDLDYAANFLHMCFSVPAENYTVDPILSRAMDRIFTLHADHEQNASTST
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGSVDRIPEFIARAKDKNDPFRLMGFGHRVYKNTDPRAKV
LKQSADEVLELLGVEDNPLLQVAKELEQTALNDPYFIEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWK
EMIGDPQNKIGRPRQLYLGETQRDYVDIENR
>Mature_430_residues
TDTQKTAQLSLNGETYELPIFSPTAGPDVLDIRKLYAQSGVFTYDPGFTSTASCDSTITYIDGNEGVLLHRGYPIDQLAG
KSHYLEVCYLLLYGELPTATQLEDFETRVTRHTMVHEQMHNFFRGFRRDAHPMATLVGVVGAMSAFYHDSTDISDPWQRE
VAAIRLIAKLPTIAAMAYKYSIGQPFVYPRNDLDYAANFLHMCFSVPAENYTVDPILSRAMDRIFTLHADHEQNASTSTV
RLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGSVDRIPEFIARAKDKNDPFRLMGFGHRVYKNTDPRAKVL
KQSADEVLELLGVEDNPLLQVAKELEQTALNDPYFIEKKLFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKE
MIGDPQNKIGRPRQLYLGETQRDYVDIENR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=351, Percent_Identity=27.3504273504274, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786939, Length=426, Percent_Identity=68.5446009389671, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=31.5363881401617, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=412, Percent_Identity=27.4271844660194, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=335, Percent_Identity=28.955223880597, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=363, Percent_Identity=27.2727272727273, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=373, Percent_Identity=26.8096514745308, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24640126, Length=349, Percent_Identity=29.2263610315186, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24640124, Length=349, Percent_Identity=29.2263610315186, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21356863, Length=430, Percent_Identity=26.2790697674419, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48280; Mature: 48149

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTQKTAQLSLNGETYELPIFSPTAGPDVLDIRKLYAQSGVFTYDPGFTSTASCDSTIT
CCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
YIDGNEGVLLHRGYPIDQLAGKSHYLEVCYLLLYGELPTATQLEDFETRVTRHTMVHEQM
EEECCCCEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
HNFFRGFRRDAHPMATLVGVVGAMSAFYHDSTDISDPWQREVAAIRLIAKLPTIAAMAYK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YSIGQPFVYPRNDLDYAANFLHMCFSVPAENYTVDPILSRAMDRIFTLHADHEQNASTST
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHEEEECCCCCCCCCCE
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGSVDRIPEFIARAKDKNDP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
FRLMGFGHRVYKNTDPRAKVLKQSADEVLELLGVEDNPLLQVAKELEQTALNDPYFIEKK
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHH
LFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKEMIGDPQNKIGRPRQLYLGE
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECC
TQRDYVDIENR
CCCCCCCCCCC
>Mature Secondary Structure 
TDTQKTAQLSLNGETYELPIFSPTAGPDVLDIRKLYAQSGVFTYDPGFTSTASCDSTIT
CCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEE
YIDGNEGVLLHRGYPIDQLAGKSHYLEVCYLLLYGELPTATQLEDFETRVTRHTMVHEQM
EEECCCCEEEECCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
HNFFRGFRRDAHPMATLVGVVGAMSAFYHDSTDISDPWQREVAAIRLIAKLPTIAAMAYK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YSIGQPFVYPRNDLDYAANFLHMCFSVPAENYTVDPILSRAMDRIFTLHADHEQNASTST
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHEEEECCCCCCCCCCE
VRLASSSGANPFACIAAGIACLWGPAHGGANQACLEMLKEIGSVDRIPEFIARAKDKNDP
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
FRLMGFGHRVYKNTDPRAKVLKQSADEVLELLGVEDNPLLQVAKELEQTALNDPYFIEKK
EEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHH
LFPNVDFYSGIILEAMGFPTSMFTPIFALSRTVGWISQWKEMIGDPQNKIGRPRQLYLGE
HCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECC
TQRDYVDIENR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]