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The map label for this gene is nuoB [H]

Identifier: 99080583

GI number: 99080583

Start: 800085

End: 800609

Strand: Direct

Name: nuoB [H]

Synonym: TM1040_0742

Alternate gene names: 99080583

Gene position: 800085-800609 (Clockwise)

Preceding gene: 99080582

Following gene: 99080584

Centisome position: 25.0

GC content: 60.95

Gene sequence:

>525_bases
ATGGCTGGCGCGAACACCGCAGGTGTCGACAAGGAAATGGTGACACAGGCCTTGAATGCAGAGCTGCAGGACAAGGGGTT
CTTGCTGACCTCTGCCGAGGACATCATCAATTGGGCGCGCACCGGTTCGCTGCACTGGATGACCTTTGGTCTTGCTTGCT
GCGCGGTGGAAATGATGCACACCTCCATGCCGCGTTACGATGCCGAGCGTTTCGGGATCGCACCGCGTGCCTCCCCGCGT
CAGTCGGACGTGATGATTGTTGCAGGTACGCTCACCAACAAAATGGCTCCGGCGTTGCGCAAGGTCTACGACCAGATGCC
CGAGCCGCGCTACGTGATCTCGATGGGGTCCTGCGCCAATGGCGGTGGCTATTACCACTACAGCTATTCCGTGGTGCGCG
GCTGTGACCGGATCGTGCCAGTGGACATCTATGTGCCTGGCTGCCCGCCCACCGCAGAGGCGCTCCTTTATGGGCTGATG
CAGCTGCAGCGCAAGATCCGCCGCACTGGCACCCTTGTGCGCTAA

Upstream 100 bases:

>100_bases
ACATCAGTGATGCGGGCTTCTGGTCGATGATGGTCTTCCTTGGGGTCCTGACCATCGGCTTTGCGTATGAGTGGAAGAAG
GGGGCTCTGGAATGGGAGTG

Downstream 100 bases:

>100_bases
GGGCAGGAGATTCAGATGATCGAAGCATTGCAAGACCTTGGCCACCAGATTGAAGCCAAGCGCCCCGAGTGCGTATTGTC
CTGGGATATTGCCCATGGCG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MAGANTAGVDKEMVTQALNAELQDKGFLLTSAEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRASPR
QSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLM
QLQRKIRRTGTLVR

Sequences:

>Translated_174_residues
MAGANTAGVDKEMVTQALNAELQDKGFLLTSAEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRASPR
QSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLM
QLQRKIRRTGTLVR
>Mature_173_residues
AGANTAGVDKEMVTQALNAELQDKGFLLTSAEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRASPRQ
SDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLMQ
LQRKIRRTGTLVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=80.1369863013699, Blast_Score=256, Evalue=8e-69,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=49.6815286624204, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=46.031746031746, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=43.4426229508197, Blast_Score=103, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=70.4225352112676, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI18859983, Length=144, Percent_Identity=77.0833333333333, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642371, Length=144, Percent_Identity=77.0833333333333, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=78.5714285714286, Blast_Score=246, Evalue=8e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19173; Mature: 19042

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
9.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
8.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGANTAGVDKEMVTQALNAELQDKGFLLTSAEDIINWARTGSLHWMTFGLACCAVEMMH
CCCCCCCCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCAN
HCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLMQLQRKIRRTGTLVR
CCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGANTAGVDKEMVTQALNAELQDKGFLLTSAEDIINWARTGSLHWMTFGLACCAVEMMH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
TSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCAN
HCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
GGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLMQLQRKIRRTGTLVR
CCCEEEEHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA