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The map label for this gene is cobT [H]

Identifier: 99080483

GI number: 99080483

Start: 686000

End: 687877

Strand: Reverse

Name: cobT [H]

Synonym: TM1040_0642

Alternate gene names: 99080483

Gene position: 687877-686000 (Counterclockwise)

Preceding gene: 99080484

Following gene: 99080482

Centisome position: 21.49

GC content: 62.35

Gene sequence:

>1878_bases
ATGAGCAAACCAAATGACAACCCGGCAGATCCGTTCAAGAAGGCCCTCGCAGAGGCCACCAAGGTCATGGCCGATGACAC
GGAACTGACGGTCAACTATTCGGTCGACCCCGCTGGAATGAGCGGCGACACGATGCGCTTGCCGCAGGTTTCCCGCCGCA
TGACACGAGAGGAAGTGCTGCTGGCGCGCGGGACCGCCGATGCGCTGGCGCTGCGCCGCAGGTATCACGACGAGAGCGTT
GCCAGCCGTTATGCGCCCTCCGGCGACATGGCGCGCGAATTGTATGATGCAATGGAAAGCGCCCGTTGCGAGGCGATGGG
CGCGCGCGATATGCCGGGCACAGCCTCCAACATTGACGTCAAGATCCAGAACGAAGCGCTGCGGCGCGGGTATGATCAGG
TCACATCTGCATCAGAGGCGCCCCTGGCGGTGTCCGCAGGTTATCTGATCCGTCATCTCGCCACCGGCCGCCCGATGCCC
GAGGCAGCGGCAAACGTCATGGAACTCTGGCGTGGCTTTATCGAAGCGCAAGCTGGCGAGACGCTCGAGAACCTAGATGA
AACCCTCTCGGATCAGGCCGCATTTGCAAAATTTGCCCGCCGGGTGATTTCTGACCTTGGCTATGGCGACCAGCTTGGCG
ACGACCCGGATGATCTCGATGACGAGATGAATGACGAGTCCGAGGAGACCGCCGAAGAAGAGGAACAGCCCGACAGCTCT
GGCCAGGACGACAGCGACGAGGAAGAGGCGGACGCCTCTCCCGAGCAGGCACAGGAACAGCAGCAGGACGAGAGTCAGGC
TCAGGTTTCCGCCGACGACATGGCCGAAGAAGAGGTCACCGACGAGACCGAGATGCCCGACGGCGAGGCCCCGCTGGAGC
CTCCGGCCCCGCAACCCGTCTCTGACGCTGATCCCGATTACCGCGTGTTCCTCGACGCCCATGACGAGGAAATCGCCGCC
GAAGATCTCGCTGAGCCACAGGAGCTCGAGCGCCTGCGCGCCTATCTCGATCAGCAGCTGGAGCCTCTGAAGGGCGCGGT
ATCGCGGCTTGCCAACAAACTGCAGCGTCGCCTGCAGGCGCAGCAGAACCGCTCTTGGGAATTCGACAAGGAAGAAGGCG
TGCTGGATGCTGGCCGCCTGGCGCGTGTGGTCGCGAACCCCACCACGCCCCTGTCGTTCAAGGTCGAAAAGGACATGGAG
TTCCGCGACACTGTGGTGACGCTCCTGTTGGACAATTCCGGCTCCATGCGCGGGCGGCCTATTTCCATCGCGGCGATCTG
TGCCGATGTTCTGGCGCGCACGCTGGAGCGGTGCAACGTGAAGGTTGAAATCCTCGGCTTCACCACCCGCGCCTGGAAAG
GCGGGCTCTCGCGCGAGGCCTGGCTCAACGAGGGCCGTCCGCAGTTGCCGGGGCGTCTCAATGATCTGCGCCACATCATC
TACAAGGGGGCCGATGCGCCCTGGCGGCGCGTGCGCCCGAACCTTGGCTTGATGATGAAAGAGGGCCTCCTGAAAGAAAA
CATCGATGGTGAAGCACTCGAATGGGCGCATCGTCGCATGGTGAACCGGCCCGAGGCGCGCAAGATCCTGATGGTGATTT
CAGACGGTGCCCCCGTTGACGATTCCACCCTCTCGGTGAACCCGGCGAATTACCTTGAAAAACACCTGCGTGACGTAATC
GCCATGGTGGAGAAAAAGAAACAGGTGGAGCTTCTCGCCATTGGCATTGGCCATGATGTGACCCGCTATTACGACCGCGC
GGTGACGATCACCGACGTCGAACAGCTTGCGGGCGCGATGACGGAACAGCTTGCGGCCCTCTTTGACAGTGATCCAAGGG
CGCGTGCGCGGGTAATGGGGATGCGTCGCACCGGATAG

Upstream 100 bases:

>100_bases
CAGGCACCCCCGCCCCTTGCGGGACGCGACCCGTCAGCCTCCGAAGGGCTGGACAGCGCCCCGCAAAGGTTCAATGCTGG
TGCAACAGGAGCCCGCGCTC

Downstream 100 bases:

>100_bases
CACCTGCGGGTCTCATGCACGGTCACAATCGACCATTCTGAGGGCGCGTCTTGGCGCGCCCTCAACTGCACTCATTGCCG
CACCATTAACGTGCCTGCGT

Product: cobalt chelatase, CobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSKPNDNPADPFKKALAEATKVMADDTELTVNYSVDPAGMSGDTMRLPQVSRRMTREEVLLARGTADALALRRRYHDESV
ASRYAPSGDMARELYDAMESARCEAMGARDMPGTASNIDVKIQNEALRRGYDQVTSASEAPLAVSAGYLIRHLATGRPMP
EAAANVMELWRGFIEAQAGETLENLDETLSDQAAFAKFARRVISDLGYGDQLGDDPDDLDDEMNDESEETAEEEEQPDSS
GQDDSDEEEADASPEQAQEQQQDESQAQVSADDMAEEEVTDETEMPDGEAPLEPPAPQPVSDADPDYRVFLDAHDEEIAA
EDLAEPQELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDKEEGVLDAGRLARVVANPTTPLSFKVEKDME
FRDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLSREAWLNEGRPQLPGRLNDLRHII
YKGADAPWRRVRPNLGLMMKEGLLKENIDGEALEWAHRRMVNRPEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVI
AMVEKKKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGAMTEQLAALFDSDPRARARVMGMRRTG

Sequences:

>Translated_625_residues
MSKPNDNPADPFKKALAEATKVMADDTELTVNYSVDPAGMSGDTMRLPQVSRRMTREEVLLARGTADALALRRRYHDESV
ASRYAPSGDMARELYDAMESARCEAMGARDMPGTASNIDVKIQNEALRRGYDQVTSASEAPLAVSAGYLIRHLATGRPMP
EAAANVMELWRGFIEAQAGETLENLDETLSDQAAFAKFARRVISDLGYGDQLGDDPDDLDDEMNDESEETAEEEEQPDSS
GQDDSDEEEADASPEQAQEQQQDESQAQVSADDMAEEEVTDETEMPDGEAPLEPPAPQPVSDADPDYRVFLDAHDEEIAA
EDLAEPQELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDKEEGVLDAGRLARVVANPTTPLSFKVEKDME
FRDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLSREAWLNEGRPQLPGRLNDLRHII
YKGADAPWRRVRPNLGLMMKEGLLKENIDGEALEWAHRRMVNRPEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVI
AMVEKKKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGAMTEQLAALFDSDPRARARVMGMRRTG
>Mature_624_residues
SKPNDNPADPFKKALAEATKVMADDTELTVNYSVDPAGMSGDTMRLPQVSRRMTREEVLLARGTADALALRRRYHDESVA
SRYAPSGDMARELYDAMESARCEAMGARDMPGTASNIDVKIQNEALRRGYDQVTSASEAPLAVSAGYLIRHLATGRPMPE
AAANVMELWRGFIEAQAGETLENLDETLSDQAAFAKFARRVISDLGYGDQLGDDPDDLDDEMNDESEETAEEEEQPDSSG
QDDSDEEEADASPEQAQEQQQDESQAQVSADDMAEEEVTDETEMPDGEAPLEPPAPQPVSDADPDYRVFLDAHDEEIAAE
DLAEPQELERLRAYLDQQLEPLKGAVSRLANKLQRRLQAQQNRSWEFDKEEGVLDAGRLARVVANPTTPLSFKVEKDMEF
RDTVVTLLLDNSGSMRGRPISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLSREAWLNEGRPQLPGRLNDLRHIIY
KGADAPWRRVRPNLGLMMKEGLLKENIDGEALEWAHRRMVNRPEARKILMVISDGAPVDDSTLSVNPANYLEKHLRDVIA
MVEKKKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGAMTEQLAALFDSDPRARARVMGMRRTG

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 69428; Mature: 69297

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPNDNPADPFKKALAEATKVMADDTELTVNYSVDPAGMSGDTMRLPQVSRRMTREEVL
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
LARGTADALALRRRYHDESVASRYAPSGDMARELYDAMESARCEAMGARDMPGTASNIDV
HHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
KIQNEALRRGYDQVTSASEAPLAVSAGYLIRHLATGRPMPEAAANVMELWRGFIEAQAGE
EECHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
TLENLDETLSDQAAFAKFARRVISDLGYGDQLGDDPDDLDDEMNDESEETAEEEEQPDSS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCC
GQDDSDEEEADASPEQAQEQQQDESQAQVSADDMAEEEVTDETEMPDGEAPLEPPAPQPV
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SDADPDYRVFLDAHDEEIAAEDLAEPQELERLRAYLDQQLEPLKGAVSRLANKLQRRLQA
CCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQNRSWEFDKEEGVLDAGRLARVVANPTTPLSFKVEKDMEFRDTVVTLLLDNSGSMRGRP
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEECCCCCCCCCC
ISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLSREAWLNEGRPQLPGRLNDLRHII
HHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHH
YKGADAPWRRVRPNLGLMMKEGLLKENIDGEALEWAHRRMVNRPEARKILMVISDGAPVD
HCCCCCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
DSTLSVNPANYLEKHLRDVIAMVEKKKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGAM
CCCEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCEEEHHHHHHHHHH
TEQLAALFDSDPRARARVMGMRRTG
HHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKPNDNPADPFKKALAEATKVMADDTELTVNYSVDPAGMSGDTMRLPQVSRRMTREEVL
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHH
LARGTADALALRRRYHDESVASRYAPSGDMARELYDAMESARCEAMGARDMPGTASNIDV
HHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
KIQNEALRRGYDQVTSASEAPLAVSAGYLIRHLATGRPMPEAAANVMELWRGFIEAQAGE
EECHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCH
TLENLDETLSDQAAFAKFARRVISDLGYGDQLGDDPDDLDDEMNDESEETAEEEEQPDSS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCC
GQDDSDEEEADASPEQAQEQQQDESQAQVSADDMAEEEVTDETEMPDGEAPLEPPAPQPV
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SDADPDYRVFLDAHDEEIAAEDLAEPQELERLRAYLDQQLEPLKGAVSRLANKLQRRLQA
CCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQNRSWEFDKEEGVLDAGRLARVVANPTTPLSFKVEKDMEFRDTVVTLLLDNSGSMRGRP
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHEEEEEECCCCCCCCCC
ISIAAICADVLARTLERCNVKVEILGFTTRAWKGGLSREAWLNEGRPQLPGRLNDLRHII
HHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHH
YKGADAPWRRVRPNLGLMMKEGLLKENIDGEALEWAHRRMVNRPEARKILMVISDGAPVD
HCCCCCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCC
DSTLSVNPANYLEKHLRDVIAMVEKKKQVELLAIGIGHDVTRYYDRAVTITDVEQLAGAM
CCCEECCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCCEEEHHHHHHHHHH
TEQLAALFDSDPRARARVMGMRRTG
HHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]