Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 99080404

GI number: 99080404

Start: 599575

End: 600474

Strand: Direct

Name: rpoH [H]

Synonym: TM1040_0563

Alternate gene names: 99080404

Gene position: 599575-600474 (Clockwise)

Preceding gene: 99080403

Following gene: 99080406

Centisome position: 18.73

GC content: 57.33

Gene sequence:

>900_bases
ATGGCAAATTATGCAAACCTTCCCGCCCCGTCTCCCGAAGGCGGACTCAATCGGTATCTGCAGGAAATTCGCAAGTTCCC
GCTTCTGGAGCCGGAAGAGGAATACATGCTGGCCAAGAGATGGGTCGAAGAGCAGGACACCGAAGCCGCGCACAAGATGG
TAACATCGCATCTGCGACTGGCAGCAAAAATTGCCATGGGATACCGGGGTTACGGGTTGCCTCAGGCAGAAGTCATTTCT
GAAGCTAATGTTGGTCTGATGCAGGCGGTAAAGCGGTTCGATCCGGAAAAAGGCTTCCGTCTGGCAACCTATGCGATGTG
GTGGATCCGCGCCTCCATTCAGGAGTATATCCTGCGGTCCTGGTCGCTGGTGAAGCTTGGGACCACATCTGCGCAGAAGA
AGCTGTTTTTCAATCTGCGCAAAGCCAAGGCCCGGATCGGTGCACTTGAGGATGGAGATCTGCGGCCCGAAGTGGTGAAG
AAGATCGCCACAGATCTTGGCGTGACCGAGGATGAGGTGATCTCCATGAACCGACGTATGTCGGGCGGCGATGCGTCGCT
CAATGCCATGGTGGGCAGCGACGGTGACAGCACCATGCAGTGGCAGGATTGGCTCGAGGATGAGGACGCCGATCAGGCGG
GAGATTACGAGGCCCGTGACGAGCTGCAAGCGCGCCGGGAGCTTCTCGCCGAGGCCATGAGCGTCCTCAACGATCGCGAG
AAAGACATTTTGACCCAGCGTCGTCTGGCCGAGCAGGCCAAGACGCTTGAAGAGCTGAGTGTCCAATATGATGTGAGCCG
GGAGCGCATTCGCCAAATCGAAGTGCGCGCCTTTGAAAAGCTACAGAAGAAAATGCGCGAGCTCGCGGCTGGCAAGGGGA
TGCTGCAGTCGAAGCTCTGA

Upstream 100 bases:

>100_bases
TGTTTGTATAACACTGGGCAAGGCGCGCTTGAAAACTGCGCTCATGACACGCACATGGGCTGAGATTGAGGTCCTTCTGT
ATTCGAAAGGGACAGTTCCG

Downstream 100 bases:

>100_bases
TCTTCTGTCTCAGAGATGTCCATTGATGCGCCAATCTCCCTCGGCGCGGACATTCGAAGGGGTCGCCCATCTCGGCGGCC
CCTTTTTTGTTTGTGCGGAC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MANYANLPAPSPEGGLNRYLQEIRKFPLLEPEEEYMLAKRWVEEQDTEAAHKMVTSHLRLAAKIAMGYRGYGLPQAEVIS
EANVGLMQAVKRFDPEKGFRLATYAMWWIRASIQEYILRSWSLVKLGTTSAQKKLFFNLRKAKARIGALEDGDLRPEVVK
KIATDLGVTEDEVISMNRRMSGGDASLNAMVGSDGDSTMQWQDWLEDEDADQAGDYEARDELQARRELLAEAMSVLNDRE
KDILTQRRLAEQAKTLEELSVQYDVSRERIRQIEVRAFEKLQKKMRELAAGKGMLQSKL

Sequences:

>Translated_299_residues
MANYANLPAPSPEGGLNRYLQEIRKFPLLEPEEEYMLAKRWVEEQDTEAAHKMVTSHLRLAAKIAMGYRGYGLPQAEVIS
EANVGLMQAVKRFDPEKGFRLATYAMWWIRASIQEYILRSWSLVKLGTTSAQKKLFFNLRKAKARIGALEDGDLRPEVVK
KIATDLGVTEDEVISMNRRMSGGDASLNAMVGSDGDSTMQWQDWLEDEDADQAGDYEARDELQARRELLAEAMSVLNDRE
KDILTQRRLAEQAKTLEELSVQYDVSRERIRQIEVRAFEKLQKKMRELAAGKGMLQSKL
>Mature_298_residues
ANYANLPAPSPEGGLNRYLQEIRKFPLLEPEEEYMLAKRWVEEQDTEAAHKMVTSHLRLAAKIAMGYRGYGLPQAEVISE
ANVGLMQAVKRFDPEKGFRLATYAMWWIRASIQEYILRSWSLVKLGTTSAQKKLFFNLRKAKARIGALEDGDLRPEVVKK
IATDLGVTEDEVISMNRRMSGGDASLNAMVGSDGDSTMQWQDWLEDEDADQAGDYEARDELQARRELLAEAMSVLNDREK
DILTQRRLAEQAKTLEELSVQYDVSRERIRQIEVRAFEKLQKKMRELAAGKGMLQSKL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=40.9420289855072, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=33.0960854092527, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=30.1652892561983, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34105; Mature: 33974

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYANLPAPSPEGGLNRYLQEIRKFPLLEPEEEYMLAKRWVEEQDTEAAHKMVTSHLRL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKIAMGYRGYGLPQAEVISEANVGLMQAVKRFDPEKGFRLATYAMWWIRASIQEYILRS
HHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKLGTTSAQKKLFFNLRKAKARIGALEDGDLRPEVVKKIATDLGVTEDEVISMNRRM
CCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
SGGDASLNAMVGSDGDSTMQWQDWLEDEDADQAGDYEARDELQARRELLAEAMSVLNDRE
CCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
KDILTQRRLAEQAKTLEELSVQYDVSRERIRQIEVRAFEKLQKKMRELAAGKGMLQSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
ANYANLPAPSPEGGLNRYLQEIRKFPLLEPEEEYMLAKRWVEEQDTEAAHKMVTSHLRL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKIAMGYRGYGLPQAEVISEANVGLMQAVKRFDPEKGFRLATYAMWWIRASIQEYILRS
HHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHC
WSLVKLGTTSAQKKLFFNLRKAKARIGALEDGDLRPEVVKKIATDLGVTEDEVISMNRRM
CCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCC
SGGDASLNAMVGSDGDSTMQWQDWLEDEDADQAGDYEARDELQARRELLAEAMSVLNDRE
CCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
KDILTQRRLAEQAKTLEELSVQYDVSRERIRQIEVRAFEKLQKKMRELAAGKGMLQSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]