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The map label for this gene is pcaQ [H]

Identifier: 99080403

GI number: 99080403

Start: 598290

End: 599228

Strand: Direct

Name: pcaQ [H]

Synonym: TM1040_0562

Alternate gene names: 99080403

Gene position: 598290-599228 (Clockwise)

Preceding gene: 99080395

Following gene: 99080404

Centisome position: 18.69

GC content: 61.45

Gene sequence:

>939_bases
ATGGTAAACTATCAGCACGCGCGTCAGGTCAAAATCCGACATATGCAGTGCTTGCTTGAGGTGGTGTCCTGTGGCAGCCT
GAAGGGTGCAGCAGAGCGGCTCTATCTCACGCAGCCCGCCATCTCCCGCACGCTCAAGGAACTCGAAGACATTCTGGGCA
GCACCTTGTTGTTGCGCAGTCGGGCTGGTGTCGAGCTGACGGAGGCAGGCGAAGTCTTTGCGCATTTTGCGCGAATGAGC
CTTGCCGCGTTGGACCATGGGCTCGAAAGCCTCGAGCGCACCCGTGGCGGGGCGCTGCAGCAGACAGTGACGATCGGGGC
CCTTCCCAGCGTTGCGGCGGGGCTGCTGCCCAGGGTGGCCGAGGGATTTCAGCGCCTTGCGCCGGGTGCTGTTTTGCATC
TCTCGGATGGGCCGCATGGGGCACTGATTGACCTTCTGCGCGGGGGCGCGGTCAGCATGGTCATCGGGCGGCTTGGAGAT
CCGGCCAGTATGCAGGGCGTGTCTTTCACGCAGGTTTATACCGAAGAAGTCGTCTGCGTCGTGCGCCCCGGCCACCCGTG
CCTCGCGGCGCCAGAACTTGACGATCTGTCGCGTTGGCCTGTGATTTTCCCGCCAGTGAGCGCAGCAATCCGACCGCAGG
TGGAGCGCTGGTTGATCAGTCATGGCCTTTCGGAGCCGCCCAATCGGATCGAGACTGTATCAGGCGCGTTTGCGCGGGTG
CATGCCCGCGACACCGACGCGATCTGGATGATCTCTCATGGGGTTGTGCGCAATGAACTCTCGGACGGGCGGTTGATACG
CCTGCCTTTTGATACCTCGCTTACCAAAGGTCCGGTAGGATTGATGCAGCGTCAGGATGCGGACCCGAGCCCCCTCGAGA
GCTTGTTTGTCAAAGCGCTCCATGAGGCGATTGAAGTCTTGGGATATGATGCGAGTTGA

Upstream 100 bases:

>100_bases
TTACAACCTGTGTGCGCATGATCGTCTCCTCCAATGGAGCAAAGGCTATCATGCAATTTCATTATTCAAAAATGAGTTAT
TGGCAGGTATGAATACCAAT

Downstream 100 bases:

>100_bases
ACGCTCGCCCGGGGTAGGGCTGGGCGTTGCCCCTCGCCGCGGGGTGTTTGCAAAGAAGATGACGAAGCCCTGTTGTTTTG
ATGCGTCTCCGGCCGGAAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MVNYQHARQVKIRHMQCLLEVVSCGSLKGAAERLYLTQPAISRTLKELEDILGSTLLLRSRAGVELTEAGEVFAHFARMS
LAALDHGLESLERTRGGALQQTVTIGALPSVAAGLLPRVAEGFQRLAPGAVLHLSDGPHGALIDLLRGGAVSMVIGRLGD
PASMQGVSFTQVYTEEVVCVVRPGHPCLAAPELDDLSRWPVIFPPVSAAIRPQVERWLISHGLSEPPNRIETVSGAFARV
HARDTDAIWMISHGVVRNELSDGRLIRLPFDTSLTKGPVGLMQRQDADPSPLESLFVKALHEAIEVLGYDAS

Sequences:

>Translated_312_residues
MVNYQHARQVKIRHMQCLLEVVSCGSLKGAAERLYLTQPAISRTLKELEDILGSTLLLRSRAGVELTEAGEVFAHFARMS
LAALDHGLESLERTRGGALQQTVTIGALPSVAAGLLPRVAEGFQRLAPGAVLHLSDGPHGALIDLLRGGAVSMVIGRLGD
PASMQGVSFTQVYTEEVVCVVRPGHPCLAAPELDDLSRWPVIFPPVSAAIRPQVERWLISHGLSEPPNRIETVSGAFARV
HARDTDAIWMISHGVVRNELSDGRLIRLPFDTSLTKGPVGLMQRQDADPSPLESLFVKALHEAIEVLGYDAS
>Mature_312_residues
MVNYQHARQVKIRHMQCLLEVVSCGSLKGAAERLYLTQPAISRTLKELEDILGSTLLLRSRAGVELTEAGEVFAHFARMS
LAALDHGLESLERTRGGALQQTVTIGALPSVAAGLLPRVAEGFQRLAPGAVLHLSDGPHGALIDLLRGGAVSMVIGRLGD
PASMQGVSFTQVYTEEVVCVVRPGHPCLAAPELDDLSRWPVIFPPVSAAIRPQVERWLISHGLSEPPNRIETVSGAFARV
HARDTDAIWMISHGVVRNELSDGRLIRLPFDTSLTKGPVGLMQRQDADPSPLESLFVKALHEAIEVLGYDAS

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=305, Percent_Identity=32.7868852459016, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1787601, Length=287, Percent_Identity=27.8745644599303, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788887, Length=238, Percent_Identity=25.2100840336134, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=214, Percent_Identity=22.4299065420561, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=219, Percent_Identity=28.310502283105, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=210, Percent_Identity=24.2857142857143, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787806, Length=214, Percent_Identity=23.8317757009346, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788297, Length=211, Percent_Identity=27.4881516587678, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33686; Mature: 33686

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYQHARQVKIRHMQCLLEVVSCGSLKGAAERLYLTQPAISRTLKELEDILGSTLLLRS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHHHH
RAGVELTEAGEVFAHFARMSLAALDHGLESLERTRGGALQQTVTIGALPSVAAGLLPRVA
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHH
EGFQRLAPGAVLHLSDGPHGALIDLLRGGAVSMVIGRLGDPASMQGVSFTQVYTEEVVCV
HHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEE
VRPGHPCLAAPELDDLSRWPVIFPPVSAAIRPQVERWLISHGLSEPPNRIETVSGAFARV
ECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEE
HARDTDAIWMISHGVVRNELSDGRLIRLPFDTSLTKGPVGLMQRQDADPSPLESLFVKAL
ECCCCCEEEEEECCHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
HEAIEVLGYDAS
HHHHHHHCCCCC
>Mature Secondary Structure
MVNYQHARQVKIRHMQCLLEVVSCGSLKGAAERLYLTQPAISRTLKELEDILGSTLLLRS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHHHH
RAGVELTEAGEVFAHFARMSLAALDHGLESLERTRGGALQQTVTIGALPSVAAGLLPRVA
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHH
EGFQRLAPGAVLHLSDGPHGALIDLLRGGAVSMVIGRLGDPASMQGVSFTQVYTEEVVCV
HHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHCEEEE
VRPGHPCLAAPELDDLSRWPVIFPPVSAAIRPQVERWLISHGLSEPPNRIETVSGAFARV
ECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEE
HARDTDAIWMISHGVVRNELSDGRLIRLPFDTSLTKGPVGLMQRQDADPSPLESLFVKAL
ECCCCCEEEEEECCHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHH
HEAIEVLGYDAS
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]