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The map label for this gene is gcvT [H]

Identifier: 99080327

GI number: 99080327

Start: 507703

End: 510210

Strand: Direct

Name: gcvT [H]

Synonym: TM1040_0486

Alternate gene names: 99080327

Gene position: 507703-510210 (Clockwise)

Preceding gene: 99080325

Following gene: 99080328

Centisome position: 15.86

GC content: 60.73

Gene sequence:

>2508_bases
ATGAAAACCCAAGTCAAAGCACTGGTCGTAGGCGGTGGCGCCGTCGGTACTTCGATCGCATATCATCTGGCCAAGGCTGG
TTGGGACGATGTCATGCTGATTGAGCGCGACGAGCTGACGTCCGGCTCCACGTGGCACGCCGCTGGCCTTTTGCCACTGT
TCAACATGTCCTATGCGACCACCCACATCCACAAATACTCGGTGGATTTCTACAAGACGCTTGAGGAAGAGACCGGCCTC
AATGCGGGCTTTGCCGTTGTCGGCAACCTGCGTATGGCGCAGACGCAGGAGCGTATGGACGAGTATATGCTCTATGCCTC
GACCGCCGAGACCTGCGATGTGGCCTATGAGTGGCTCACTCCGGAGCAGATCAAGGAACGCTGGCCGCTCATTGAGACCG
GCGACCTCAAAGGCGCGATCTACCACACCGAGGATGGCTATATTAACCCCGCAGATGTGACCCAGGCGATGGCCAAGGGC
GCACGTCAGCGTGGTGTTGAGATCGTCCGCAAGATGCAGGCCGACGCCTTCCACTGGAACGGTACCCATTGGGAAGTGAC
CTGCACCGTGATGGCGGAAAAGGGCGGCAATCTCGTGGAGACAGACGAGCAGGTGGTGATCACTGCCGAGCACGTTGTCA
CAGCCTCCGGCAACCACGCGCAGCGCACGGCCAAGATGCTTGGCATCAAGATGCCCGCAATCCCGGTCGAGCACACGTTC
ATCGTGATGGACAAAGATCCCGAACTGGTCAAATGGCGCGAAGCGGGCAACCCCGAGCACCCGGTTATCCGCGACGCCGA
CAATGAATCCTATGCCCGCGAAGAGCGCGGTGGCTGGATCCTTGGCATCTACGAGCACGGCGCCCCGGCGCGGTTTGAGC
ATGGCGTACCGGACAGCTTCCGTGCAGATCTGTTCCCGCTTGATCTGGACCGGATTGCGGAACAATACATGGCGATGGCG
GAGCGTGTGCCCTCCTGTGCTGACAGTGGTCTCAAGGACGATTTCAACGGCCCGATCTGCTACACGCCTGATGGCAACCC
GCTGGTCGGCCCGGCGCCGGGTCTGCGCAACATGTGGCTGGCCGAAGGCTTCTCCTTCGGGATCACCGCTGCAGGCGGCA
CCGGCTATTACCTCGCGCAGATGATGGTCGATGGCGAAGCCGAGATCGACATGGCGTCGCTTGATCCCAAACGCTACTCC
TCCAACTGGATGACCACAGAGTTCGCGGCCCGCAAGAACGAAGAGTGCTACGAGCACGTCTACATTCTGCACCACCCGGA
CGAAGAACGCCCCGCTGCGCGCCCGCTGCGCACCGCGCCTGCATTCGACCGCCAGAAAGCGCGCGGTGCACAGTTTGGCT
GGGTCAATGGATGGGAACGTCCGAACTACTTCGGCCCGGTGGATGCACCGGACAATTTTGACGAAGAAGCACGCTCCTTC
CGTCGTGGTGGCTGGTGGCAGCATGCCGTGGACGAAGCGAAGGCAATCCGCGAGGGCGTTGGCCTCATTGACGCGACCGC
CTTTACGAAACATGTCGTCAAAGGCCCCGGTGCCACCCAGTTCCTCGACTGGTTCACCTGCAACAAGCTCCCGAAGGTTG
GCCGCATCAACCTGACCTATGCGCTGACCGCATTCGGTACCACCCGCACCGAATACACCATCGTGCGCAACGGCGAGAAC
AACTACTATCTGGTCTCCGCCGGTGCGTGGTCCGAATATGACGCCGACTTCCTGCGCAAGGCGGCCGAGGACAAGATGGA
GGAATTCGGCTACATCGAGATCCAGGATGTGACAACCCAGTGGGGCGTCTTTGCCATCGCAGGGCCCAAGTCCCGCGATG
TGCTGAAAGAGGTCATCGTGGATGCCGATCCGGCGACTGCGCTCTCCAACAAGCGCTTCCCGTGGCTGTCGGCCAAGCAG
ATCGAACTGGGCATGTGCCCGGTGAACGCGATCCGCGTGGCCTATACTGGTGAACTGGGCTGGGAACTCCACCACCCGAT
CGAGATGCAGAACTACCTCTTCGACCTCCTGGAAAAAGCGGGCGAGAAACACGGTATGAAGCTCGTGGGCGCACGCGCTC
AGAACTGGCTGCGTCAGGAGAAATCCTACCGCGCCTTTGGCAACGAACTGGGCCGTGACGCGACCCCACTCGAAGCCGAC
CTGCCGCGCTTTGTCGACCTGGAGAAGGACTTCCACGGCAAGGACAAGCTGGTCGAAACCGGCGTCCGCGTGAAGTGCTG
TACCCTGCTGATCGATGGGCCAGAAGACGCCGATCCCTGGGGTCGCGAGGCGCTCTATGCCGAGGATGGCACCCGTGTGG
GCCGCCTGACCTCGGGTGGCTACTCGGTCGCCTTCGAGAAATCCATCGGCATGGGCTACGTCAAACCCGAGATGGCTGTC
GAAGGCACCAAACTGAAAGTGAAGATCCAGGATAAGCTCTGGGATGCGGTTGTCACTTGCGACAGCCCCTATGATCCCAA
GAACGAAACCATCCGAAAGGACGGTTGA

Upstream 100 bases:

>100_bases
CGGTTTTGTCATTTTTTGCGTCGGACTGATTTGCCGCCTTGGGCGCTCTGTGCGTCATAACCGATTCAATTGTGAATCAG
AGAACTCGGAGAGCGGGCTC

Downstream 100 bases:

>100_bases
TCCCTACCATCTTCGTCCGCGCCCTGTGGCGCTGGGCTTTAAGGTCGCACAAGCGCTCCGGTTTTGCCGGGGCGCTTTTC
CCTTGGTCCTGCGACCAATT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 835; Mature: 835

Protein sequence:

>835_residues
MKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERDELTSGSTWHAAGLLPLFNMSYATTHIHKYSVDFYKTLEEETGL
NAGFAVVGNLRMAQTQERMDEYMLYASTAETCDVAYEWLTPEQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAMAKG
ARQRGVEIVRKMQADAFHWNGTHWEVTCTVMAEKGGNLVETDEQVVITAEHVVTASGNHAQRTAKMLGIKMPAIPVEHTF
IVMDKDPELVKWREAGNPEHPVIRDADNESYAREERGGWILGIYEHGAPARFEHGVPDSFRADLFPLDLDRIAEQYMAMA
ERVPSCADSGLKDDFNGPICYTPDGNPLVGPAPGLRNMWLAEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPKRYS
SNWMTTEFAARKNEECYEHVYILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNGWERPNYFGPVDAPDNFDEEARSF
RRGGWWQHAVDEAKAIREGVGLIDATAFTKHVVKGPGATQFLDWFTCNKLPKVGRINLTYALTAFGTTRTEYTIVRNGEN
NYYLVSAGAWSEYDADFLRKAAEDKMEEFGYIEIQDVTTQWGVFAIAGPKSRDVLKEVIVDADPATALSNKRFPWLSAKQ
IELGMCPVNAIRVAYTGELGWELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWLRQEKSYRAFGNELGRDATPLEAD
LPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDADPWGREALYAEDGTRVGRLTSGGYSVAFEKSIGMGYVKPEMAV
EGTKLKVKIQDKLWDAVVTCDSPYDPKNETIRKDG

Sequences:

>Translated_835_residues
MKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERDELTSGSTWHAAGLLPLFNMSYATTHIHKYSVDFYKTLEEETGL
NAGFAVVGNLRMAQTQERMDEYMLYASTAETCDVAYEWLTPEQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAMAKG
ARQRGVEIVRKMQADAFHWNGTHWEVTCTVMAEKGGNLVETDEQVVITAEHVVTASGNHAQRTAKMLGIKMPAIPVEHTF
IVMDKDPELVKWREAGNPEHPVIRDADNESYAREERGGWILGIYEHGAPARFEHGVPDSFRADLFPLDLDRIAEQYMAMA
ERVPSCADSGLKDDFNGPICYTPDGNPLVGPAPGLRNMWLAEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPKRYS
SNWMTTEFAARKNEECYEHVYILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNGWERPNYFGPVDAPDNFDEEARSF
RRGGWWQHAVDEAKAIREGVGLIDATAFTKHVVKGPGATQFLDWFTCNKLPKVGRINLTYALTAFGTTRTEYTIVRNGEN
NYYLVSAGAWSEYDADFLRKAAEDKMEEFGYIEIQDVTTQWGVFAIAGPKSRDVLKEVIVDADPATALSNKRFPWLSAKQ
IELGMCPVNAIRVAYTGELGWELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWLRQEKSYRAFGNELGRDATPLEAD
LPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDADPWGREALYAEDGTRVGRLTSGGYSVAFEKSIGMGYVKPEMAV
EGTKLKVKIQDKLWDAVVTCDSPYDPKNETIRKDG
>Mature_835_residues
MKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERDELTSGSTWHAAGLLPLFNMSYATTHIHKYSVDFYKTLEEETGL
NAGFAVVGNLRMAQTQERMDEYMLYASTAETCDVAYEWLTPEQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAMAKG
ARQRGVEIVRKMQADAFHWNGTHWEVTCTVMAEKGGNLVETDEQVVITAEHVVTASGNHAQRTAKMLGIKMPAIPVEHTF
IVMDKDPELVKWREAGNPEHPVIRDADNESYAREERGGWILGIYEHGAPARFEHGVPDSFRADLFPLDLDRIAEQYMAMA
ERVPSCADSGLKDDFNGPICYTPDGNPLVGPAPGLRNMWLAEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPKRYS
SNWMTTEFAARKNEECYEHVYILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNGWERPNYFGPVDAPDNFDEEARSF
RRGGWWQHAVDEAKAIREGVGLIDATAFTKHVVKGPGATQFLDWFTCNKLPKVGRINLTYALTAFGTTRTEYTIVRNGEN
NYYLVSAGAWSEYDADFLRKAAEDKMEEFGYIEIQDVTTQWGVFAIAGPKSRDVLKEVIVDADPATALSNKRFPWLSAKQ
IELGMCPVNAIRVAYTGELGWELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWLRQEKSYRAFGNELGRDATPLEAD
LPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDADPWGREALYAEDGTRVGRLTSGGYSVAFEKSIGMGYVKPEMAV
EGTKLKVKIQDKLWDAVVTCDSPYDPKNETIRKDG

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI24797151, Length=850, Percent_Identity=35.8823529411765, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI197927446, Length=885, Percent_Identity=28.8135593220339, Blast_Score=313, Evalue=4e-85,
Organism=Homo sapiens, GI21361378, Length=885, Percent_Identity=28.8135593220339, Blast_Score=313, Evalue=4e-85,
Organism=Homo sapiens, GI194306651, Length=816, Percent_Identity=29.4117647058824, Blast_Score=301, Evalue=2e-81,
Organism=Escherichia coli, GI1789272, Length=304, Percent_Identity=24.3421052631579, Blast_Score=63, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71994045, Length=840, Percent_Identity=27.8571428571429, Blast_Score=291, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71994052, Length=847, Percent_Identity=27.6269185360094, Blast_Score=288, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI32563613, Length=864, Percent_Identity=26.0416666666667, Blast_Score=236, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6320222, Length=339, Percent_Identity=27.1386430678466, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI28571104, Length=830, Percent_Identity=30.1204819277108, Blast_Score=312, Evalue=7e-85,
Organism=Drosophila melanogaster, GI20130091, Length=876, Percent_Identity=27.9680365296804, Blast_Score=279, Evalue=6e-75,
Organism=Drosophila melanogaster, GI20129441, Length=292, Percent_Identity=26.7123287671233, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 93316; Mature: 93316

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERDELTSGSTWHAAGLLPLFNMSYAT
CCCEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCHHHHCCCHHE
THIHKYSVDFYKTLEEETGLNAGFAVVGNLRMAQTQERMDEYMLYASTAETCDVAYEWLT
EEEEEECHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHEEEECCCCHHHHEEECCC
PEQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAMAKGARQRGVEIVRKMQADAFHWN
HHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
GTHWEVTCTVMAEKGGNLVETDEQVVITAEHVVTASGNHAQRTAKMLGIKMPAIPVEHTF
CCEEEEEEEEEECCCCCEEECCCEEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCCEEE
IVMDKDPELVKWREAGNPEHPVIRDADNESYAREERGGWILGIYEHGAPARFEHGVPDSF
EEECCCCCEEEHHHCCCCCCCEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
RADLFPLDLDRIAEQYMAMAERVPSCADSGLKDDFNGPICYTPDGNPLVGPAPGLRNMWL
CCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHH
AEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPKRYSSNWMTTEFAARKNEECYEHV
HCCCCCEEEECCCCCEEEEEEEECCCCCEEEECCCCHHCCCCCCEEHHHHCCCCCHHHEE
YILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNGWERPNYFGPVDAPDNFDEEARSF
EEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RRGGWWQHAVDEAKAIREGVGLIDATAFTKHVVKGPGATQFLDWFTCNKLPKVGRINLTY
HCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEE
ALTAFGTTRTEYTIVRNGENNYYLVSAGAWSEYDADFLRKAAEDKMEEFGYIEIQDVTTQ
EEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
WGVFAIAGPKSRDVLKEVIVDADPATALSNKRFPWLSAKQIELGMCPVNAIRVAYTGELG
CCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEECCCCEEEEEEEECCCC
WELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWLRQEKSYRAFGNELGRDATPLEAD
CEECCCHHHHHHHHHHHHHHCHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDADPWGREALYAEDGTRVGRLTSGG
CCHHEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCC
YSVAFEKSIGMGYVKPEMAVEGTKLKVKIQDKLWDAVVTCDSPYDPKNETIRKDG
EEEEEECCCCCCEECCCCEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTQVKALVVGGGAVGTSIAYHLAKAGWDDVMLIERDELTSGSTWHAAGLLPLFNMSYAT
CCCEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCCHHHHCCCHHE
THIHKYSVDFYKTLEEETGLNAGFAVVGNLRMAQTQERMDEYMLYASTAETCDVAYEWLT
EEEEEECHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHEEEECCCCHHHHEEECCC
PEQIKERWPLIETGDLKGAIYHTEDGYINPADVTQAMAKGARQRGVEIVRKMQADAFHWN
HHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEC
GTHWEVTCTVMAEKGGNLVETDEQVVITAEHVVTASGNHAQRTAKMLGIKMPAIPVEHTF
CCEEEEEEEEEECCCCCEEECCCEEEEEEEEEEECCCCHHHHHHHHHHEECCCCCCCEEE
IVMDKDPELVKWREAGNPEHPVIRDADNESYAREERGGWILGIYEHGAPARFEHGVPDSF
EEECCCCCEEEHHHCCCCCCCEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
RADLFPLDLDRIAEQYMAMAERVPSCADSGLKDDFNGPICYTPDGNPLVGPAPGLRNMWL
CCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHH
AEGFSFGITAAGGTGYYLAQMMVDGEAEIDMASLDPKRYSSNWMTTEFAARKNEECYEHV
HCCCCCEEEECCCCCEEEEEEEECCCCCEEEECCCCHHCCCCCCEEHHHHCCCCCHHHEE
YILHHPDEERPAARPLRTAPAFDRQKARGAQFGWVNGWERPNYFGPVDAPDNFDEEARSF
EEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
RRGGWWQHAVDEAKAIREGVGLIDATAFTKHVVKGPGATQFLDWFTCNKLPKVGRINLTY
HCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEE
ALTAFGTTRTEYTIVRNGENNYYLVSAGAWSEYDADFLRKAAEDKMEEFGYIEIQDVTTQ
EEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
WGVFAIAGPKSRDVLKEVIVDADPATALSNKRFPWLSAKQIELGMCPVNAIRVAYTGELG
CCEEEEECCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEEEECCCCEEEEEEEECCCC
WELHHPIEMQNYLFDLLEKAGEKHGMKLVGARAQNWLRQEKSYRAFGNELGRDATPLEAD
CEECCCHHHHHHHHHHHHHHCHHCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LPRFVDLEKDFHGKDKLVETGVRVKCCTLLIDGPEDADPWGREALYAEDGTRVGRLTSGG
CCHHEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCC
YSVAFEKSIGMGYVKPEMAVEGTKLKVKIQDKLWDAVVTCDSPYDPKNETIRKDG
EEEEEECCCCCCEECCCCEECCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA