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The map label for this gene is ycjM [H]

Identifier: 99080259

GI number: 99080259

Start: 428354

End: 430087

Strand: Direct

Name: ycjM [H]

Synonym: TM1040_0418

Alternate gene names: 99080259

Gene position: 428354-430087 (Clockwise)

Preceding gene: 99080257

Following gene: 99080260

Centisome position: 13.38

GC content: 59.46

Gene sequence:

>1734_bases
ATGGCCACATCCTTTGCTGCACGCCTGTCGCAACTGCTGACCCAAATCTATCCGGATTTGGACACGGAGATCCTTGGCTC
CAAAGTGGTTGAGGCGTTTTGGCCCGAAGGCAGTCACCGGCGCAAGCGCCCGCGCAGGCCTGGAAATTCTCTTTGGTCAG
AACGCGACGCATTGCTGATCACCTATGGCAACACCATGCGTGATGGGGCGCATAAGCCGTTAGACCTGCTGCATGATTTC
CTGCTGACTTATATGAAGGGTGTTGTGAATGGCGTGCATATCCTGCCGTTTTTTCCCTTCACTTCGGACGACGGGTTTGC
CGTCACGGACTATCGCAAGGTGAATCCTGAGCTGGGTGATTGGGCCGACATTCGCCGGATCGGCGGCGCGTTTCACCTGA
TGTCTGACATGGTGCTCAACCATGTGTCCTCGCAGAGCGCCTGGTTCAACGCCTACCGGCAGGGGCAGCCGCCTTATGAC
CGCTTCTTCTACGAGGCTTCGCCCTCGGACGATCTGAGCGCAGTGGTGCGTCCACGCACAACGCCGCTGCTGCAGGAGGT
AGAGACAGCCACGGGCGCGAAACATGTGTGGTGCACCTTCAGCCACGATCAGGTTGATCTCAATTTTGAGAACCCGGAGG
TCCTGCTGGAAATCCTTCGGATCATTCGCCTGCATATCGATCAGGGGGTCCGCATTATCCGGCTCGATGCGGTGGCCTTT
ATATGGAAAGAGGTTGGGACCAGTTCGATCCACCTGCCGCAAACCCATGCGATTGTACAGCTGCTGCGCCTGCTGGCAGA
TTATGCGACCGAGACGGTGGTACTGCTGACCGAGACCAACGTGCCGCGGGCTGAGAATCTCAGCTACTTTGGCAATCGCA
ACGAGGCCCATGTGGTCTATAATTTTCCGCTGCCGCCATTGATCCTGCACGCGATGATGGCGGGCTCGGCGCGCTACCTG
CTGAATTGGGCACGGGCGATGCCGCCGGCGCCCCTGGGATGCGCCTATTTGAATTTCACCGCGAGCCACGATGGAATCGG
GATGCGCCCGGCGGAGGGGGTGTTGCCGCAGGAGGAAATCGACCAGATGATCGCCTGCGTGCGGGCGGTAGGGGGTCTTG
TGTCCATGCGGGCCTTGCCGGGGGGTGGTGAAGCGCCCTACGAGGTGAACTGCACCTATTTTGACGCGCTTGGCCGAACC
TTTGACAGGGGCGAAGCGCGAAAGGTGGATCGATTCATCTGTGCGCAGACCATTCCCATGAGCCTTGAGGGAATTCCGGC
GTTTTACATTCACGCGATGCTGGCGACGGCCAATGATCATGATGCGGTGGCGCGGCGCGGTATGAACCGGGCGATCAACC
GCCACCGGTGGGATTACGGCGAGCTGAAGGCGCGTCTGAATGACGCGGACAGCGCGCAGGCTCAGGTGATGTCGGCGCTC
TCCGAACGGCTGCGGGTCCGGGCCGAGCAGCCGGCGTTTCACCCCAATGCTACCCAGTTCACTTTGCAGCTGGATGATCG
TGTCTTTGCGCTCTGGCGGCAGTCGCTGGACCGGGCGCAGTCGATCTTTGCGCTGCACAATGTCAGCGGAGATGGGGTGA
TCCTGCATCCCGGCGCGCTGAACCTTATTGAAGGTGAGACATGGCGGGATCTGTTGTCCGGTGACATGTTTGAAAGCGAT
GCAGAGATCACACTGGCACCCTATCAATGCCGTTGGATCACCAATCAGGCTTGA

Upstream 100 bases:

>100_bases
GCACAGAATTTGCCCGCGTGACCGTAGGCTCCTAGGGTTACAGAGGACCCTGTCTCACGGTGAGGGAGGCATCTGCCGCG
CTATCGCAAAAGGGAGAAAG

Downstream 100 bases:

>100_bases
GCGGCGGCGCGGGAGAATGTTAGAGGAGCGCAGACGCCCCTCCAGACAAGGGAATTGTTGATGTTGGAGATTGCACCCAC
CCGCTGACAGGATCCGCACT

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MATSFAARLSQLLTQIYPDLDTEILGSKVVEAFWPEGSHRRKRPRRPGNSLWSERDALLITYGNTMRDGAHKPLDLLHDF
LLTYMKGVVNGVHILPFFPFTSDDGFAVTDYRKVNPELGDWADIRRIGGAFHLMSDMVLNHVSSQSAWFNAYRQGQPPYD
RFFYEASPSDDLSAVVRPRTTPLLQEVETATGAKHVWCTFSHDQVDLNFENPEVLLEILRIIRLHIDQGVRIIRLDAVAF
IWKEVGTSSIHLPQTHAIVQLLRLLADYATETVVLLTETNVPRAENLSYFGNRNEAHVVYNFPLPPLILHAMMAGSARYL
LNWARAMPPAPLGCAYLNFTASHDGIGMRPAEGVLPQEEIDQMIACVRAVGGLVSMRALPGGGEAPYEVNCTYFDALGRT
FDRGEARKVDRFICAQTIPMSLEGIPAFYIHAMLATANDHDAVARRGMNRAINRHRWDYGELKARLNDADSAQAQVMSAL
SERLRVRAEQPAFHPNATQFTLQLDDRVFALWRQSLDRAQSIFALHNVSGDGVILHPGALNLIEGETWRDLLSGDMFESD
AEITLAPYQCRWITNQA

Sequences:

>Translated_577_residues
MATSFAARLSQLLTQIYPDLDTEILGSKVVEAFWPEGSHRRKRPRRPGNSLWSERDALLITYGNTMRDGAHKPLDLLHDF
LLTYMKGVVNGVHILPFFPFTSDDGFAVTDYRKVNPELGDWADIRRIGGAFHLMSDMVLNHVSSQSAWFNAYRQGQPPYD
RFFYEASPSDDLSAVVRPRTTPLLQEVETATGAKHVWCTFSHDQVDLNFENPEVLLEILRIIRLHIDQGVRIIRLDAVAF
IWKEVGTSSIHLPQTHAIVQLLRLLADYATETVVLLTETNVPRAENLSYFGNRNEAHVVYNFPLPPLILHAMMAGSARYL
LNWARAMPPAPLGCAYLNFTASHDGIGMRPAEGVLPQEEIDQMIACVRAVGGLVSMRALPGGGEAPYEVNCTYFDALGRT
FDRGEARKVDRFICAQTIPMSLEGIPAFYIHAMLATANDHDAVARRGMNRAINRHRWDYGELKARLNDADSAQAQVMSAL
SERLRVRAEQPAFHPNATQFTLQLDDRVFALWRQSLDRAQSIFALHNVSGDGVILHPGALNLIEGETWRDLLSGDMFESD
AEITLAPYQCRWITNQA
>Mature_576_residues
ATSFAARLSQLLTQIYPDLDTEILGSKVVEAFWPEGSHRRKRPRRPGNSLWSERDALLITYGNTMRDGAHKPLDLLHDFL
LTYMKGVVNGVHILPFFPFTSDDGFAVTDYRKVNPELGDWADIRRIGGAFHLMSDMVLNHVSSQSAWFNAYRQGQPPYDR
FFYEASPSDDLSAVVRPRTTPLLQEVETATGAKHVWCTFSHDQVDLNFENPEVLLEILRIIRLHIDQGVRIIRLDAVAFI
WKEVGTSSIHLPQTHAIVQLLRLLADYATETVVLLTETNVPRAENLSYFGNRNEAHVVYNFPLPPLILHAMMAGSARYLL
NWARAMPPAPLGCAYLNFTASHDGIGMRPAEGVLPQEEIDQMIACVRAVGGLVSMRALPGGGEAPYEVNCTYFDALGRTF
DRGEARKVDRFICAQTIPMSLEGIPAFYIHAMLATANDHDAVARRGMNRAINRHRWDYGELKARLNDADSAQAQVMSALS
ERLRVRAEQPAFHPNATQFTLQLDDRVFALWRQSLDRAQSIFALHNVSGDGVILHPGALNLIEGETWRDLLSGDMFESDA
EITLAPYQCRWITNQA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=570, Percent_Identity=39.8245614035088, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1790687, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 64837; Mature: 64705

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSFAARLSQLLTQIYPDLDTEILGSKVVEAFWPEGSHRRKRPRRPGNSLWSERDALLI
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCEEEE
TYGNTMRDGAHKPLDLLHDFLLTYMKGVVNGVHILPFFPFTSDDGFAVTDYRKVNPELGD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCCC
WADIRRIGGAFHLMSDMVLNHVSSQSAWFNAYRQGQPPYDRFFYEASPSDDLSAVVRPRT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHCCCC
TPLLQEVETATGAKHVWCTFSHDQVDLNFENPEVLLEILRIIRLHIDQGVRIIRLDAVAF
CHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
IWKEVGTSSIHLPQTHAIVQLLRLLADYATETVVLLTETNVPRAENLSYFGNRNEAHVVY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHCCCCCCEEEEE
NFPLPPLILHAMMAGSARYLLNWARAMPPAPLGCAYLNFTASHDGIGMRPAEGVLPQEEI
ECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
DQMIACVRAVGGLVSMRALPGGGEAPYEVNCTYFDALGRTFDRGEARKVDRFICAQTIPM
HHHHHHHHHHHHHHEEHCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCC
SLEGIPAFYIHAMLATANDHDAVARRGMNRAINRHRWDYGELKARLNDADSAQAQVMSAL
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
SERLRVRAEQPAFHPNATQFTLQLDDRVFALWRQSLDRAQSIFALHNVSGDGVILHPGAL
HHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
NLIEGETWRDLLSGDMFESDAEITLAPYQCRWITNQA
EECCCCHHHHHHCCCCCCCCCCEEECCEEEEEECCCC
>Mature Secondary Structure 
ATSFAARLSQLLTQIYPDLDTEILGSKVVEAFWPEGSHRRKRPRRPGNSLWSERDALLI
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCEEEE
TYGNTMRDGAHKPLDLLHDFLLTYMKGVVNGVHILPFFPFTSDDGFAVTDYRKVNPELGD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCCC
WADIRRIGGAFHLMSDMVLNHVSSQSAWFNAYRQGQPPYDRFFYEASPSDDLSAVVRPRT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHCCCC
TPLLQEVETATGAKHVWCTFSHDQVDLNFENPEVLLEILRIIRLHIDQGVRIIRLDAVAF
CHHHHHHHHHCCCCEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHH
IWKEVGTSSIHLPQTHAIVQLLRLLADYATETVVLLTETNVPRAENLSYFGNRNEAHVVY
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHCCCCCCEEEEE
NFPLPPLILHAMMAGSARYLLNWARAMPPAPLGCAYLNFTASHDGIGMRPAEGVLPQEEI
ECCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHH
DQMIACVRAVGGLVSMRALPGGGEAPYEVNCTYFDALGRTFDRGEARKVDRFICAQTIPM
HHHHHHHHHHHHHHEEHCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHCCCC
SLEGIPAFYIHAMLATANDHDAVARRGMNRAINRHRWDYGELKARLNDADSAQAQVMSAL
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
SERLRVRAEQPAFHPNATQFTLQLDDRVFALWRQSLDRAQSIFALHNVSGDGVILHPGAL
HHHHHHHCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
NLIEGETWRDLLSGDMFESDAEITLAPYQCRWITNQA
EECCCCHHHHHHCCCCCCCCCCEEECCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]