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The map label for this gene is cocE [H]

Identifier: 99080232

GI number: 99080232

Start: 399046

End: 401052

Strand: Direct

Name: cocE [H]

Synonym: TM1040_0391

Alternate gene names: 99080232

Gene position: 399046-401052 (Clockwise)

Preceding gene: 99080230

Following gene: 99080238

Centisome position: 12.47

GC content: 61.83

Gene sequence:

>2007_bases
ATGACACAGCCCTCTGCCATCACCGTGACCGAACATTTGTGGATTCCCCTGCCTGATGGCCGCAGGCTTGCGGCGCGGAT
GTGGATGCCCGAAGGTAAAGGTCCGTTTCCTGTCATTCTTGAGTATCTGCCTTACCGCAAACGCGATGGCACGGCGCCGC
GCGATGCCACGACGCATCCGGTCTTTGCCGCAGAGGGCTATGCTTGCCTGCGTGTGGACATCGCAGGCTCTGGCGATAGT
GACGGGCGGTTTGACGACGAATATTCCGAGCAGGAACTGTCGGATGGTGAGGCAGTGCTGGAGTGGATCGCGGCGCAGCC
CTGGTCGAGCGGAAATGTCGGTATGATCGGGATTTCCTGGGGTGGCTTCAATGGCTTGCAGATGGCCTACCGCCGCCCCG
AGGCGCTAAAGGCGGTGGTCTCGGTGGCCTCTACCGTGGATCGCTACGCCGATGATATTCATTATATGGGGGGCTGTCTT
CTTAGCGACAACAAGAATTGGGCGAGCCAGATGTTTGCCTATATGACGCGACCGCTGGACCCGAAACTGCGTCCGGATTG
GCGGGAGGAATGGCTCGGCCGTATCAACGATCTTCCTTTTATGGCCGCTGATTGGCTGAAGCACCCAACCCGGGACACGT
TCTGGAAACATGGGTCCGTCAGTGAGGACTGGTCGGCGATCAAGGCGCCGGTTCTGGCGATCACGGGCTGGGCCGATGCC
TATGTGAACGCACCATCTGCGCTCGCCGCCAAGATGACTGGCGCCCCTGCCAAAGCCTTGATGGGGCCGTGGGAGCATCG
CTATGCACATATTTCCCAGATCGAGGCGGCGGATTTTCACGGTGAGGTCCTGCGCTGGTTCGATCACTATCTCAAAGGCG
AGGACGCCGGGGCAGACGCGCTGCCGGATTACCGTGTCTTTCTGCAAGAGTTTACCGAGCCGAGCCCCAAGAACCTGCCG
CAGAACGGCCGGTGGGTGGCCGAGGCGCATTGGCCGTCGCCCAATGTCAGCGATGTGACATGGCCGCTGGCGCGTGCGGG
ATTTGCGGCTGCCCCGCAGGCGGGAACGGTCGTGGTCAAGAGCGCGGCAACCGTGGGGCAGGCGGGCGGGTATTTTTGCC
CCGGGATCCGGATCGACAACGAACGTCCTGGTGATCAGAGCGTGGATGACGCGGCCTCGACCTGTTTTGACAGCACACCG
CTCCTGGCGCCGATGGAGATCCTGGGCCGCCCGCGTGTGCGCTTGGCCTTTAGTGTCGACAAACCCGTGGCGCAGGTGGT
GGCGCGGCTTTGTGATGTGGCGCCTTCGGGGCAATCGCAGCGTATCACCTATCGACCGCGGAACCTATGTCATGATGACA
GTCATGAGGTCGCCACCAAACTCGAACCCGGCAAGCGCTATGAGGTGGAGTTTGAGCTCAATGAATGTGCCCATCGTGTG
CTGGCGGGGCATCGACTGAGGCTTGCGCTCTCGACCTCGTATTGGCCAATTGTCTGGCCCGCGCCGGAAGCGGCGGAGAT
TATGCTGCATCTGGAAGACTGCGCGCTCGTCCTGCCGGAGCGCCGCGTCGAGGTTGAGATTGACGCGCAGGCCCCGGGCG
CGGCGCGGGACTACCCGAGGTATCAAGCACAGGTGTTGGAAAAGCCCTGGGGCGAGGCAACAACCAGGATCGAGGCGGAT
GGAACCCATGTTCTGGAGAGCTATGACAGCTACGGGGCCGCGCGCGATCTCTCCCATGGCCAGCAGGTGAGCAGCAATGT
GCGGATGCGCTATGCCATCCACCCGGATGATCCGGCCAGCGCGCGGTTTGATTGCGCCTGGGTCTTTGCCTTTGGGCGCG
AGACAGACGGGGATGCTCCGGATTGGAACCTGCGCATCGAGACCGACAGCGCGATGTGGTGTGACACCGAAACTTTCACG
CTCTGGCGCGAGATCAAGGCCTTTGAGGGCGAAGACTTGATCCTGACTCGTGACTGGAAAGAGGTGATCCCGCGCGGCTG
TCACTAG

Upstream 100 bases:

>100_bases
CTTGCGTACATATGCATCATGTCGCGTACAAGCGCGCAGTTTCCCCGGCCCGACATTTCGCGCAGAGTTTGCAGACATCG
CCAAGACCGAAGAGAAATCT

Downstream 100 bases:

>100_bases
GCGCGGCGGTTACAAAAGAGCCCACCGCGTGCGGCGGTGGGCGTCATTCTGCGTTGAGCGATCGCTCAGGATTGTGGCAG
GGCGCGGGGGTCGAACTGGC

Product: peptidase S15

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MTQPSAITVTEHLWIPLPDGRRLAARMWMPEGKGPFPVILEYLPYRKRDGTAPRDATTHPVFAAEGYACLRVDIAGSGDS
DGRFDDEYSEQELSDGEAVLEWIAAQPWSSGNVGMIGISWGGFNGLQMAYRRPEALKAVVSVASTVDRYADDIHYMGGCL
LSDNKNWASQMFAYMTRPLDPKLRPDWREEWLGRINDLPFMAADWLKHPTRDTFWKHGSVSEDWSAIKAPVLAITGWADA
YVNAPSALAAKMTGAPAKALMGPWEHRYAHISQIEAADFHGEVLRWFDHYLKGEDAGADALPDYRVFLQEFTEPSPKNLP
QNGRWVAEAHWPSPNVSDVTWPLARAGFAAAPQAGTVVVKSAATVGQAGGYFCPGIRIDNERPGDQSVDDAASTCFDSTP
LLAPMEILGRPRVRLAFSVDKPVAQVVARLCDVAPSGQSQRITYRPRNLCHDDSHEVATKLEPGKRYEVEFELNECAHRV
LAGHRLRLALSTSYWPIVWPAPEAAEIMLHLEDCALVLPERRVEVEIDAQAPGAARDYPRYQAQVLEKPWGEATTRIEAD
GTHVLESYDSYGAARDLSHGQQVSSNVRMRYAIHPDDPASARFDCAWVFAFGRETDGDAPDWNLRIETDSAMWCDTETFT
LWREIKAFEGEDLILTRDWKEVIPRGCH

Sequences:

>Translated_668_residues
MTQPSAITVTEHLWIPLPDGRRLAARMWMPEGKGPFPVILEYLPYRKRDGTAPRDATTHPVFAAEGYACLRVDIAGSGDS
DGRFDDEYSEQELSDGEAVLEWIAAQPWSSGNVGMIGISWGGFNGLQMAYRRPEALKAVVSVASTVDRYADDIHYMGGCL
LSDNKNWASQMFAYMTRPLDPKLRPDWREEWLGRINDLPFMAADWLKHPTRDTFWKHGSVSEDWSAIKAPVLAITGWADA
YVNAPSALAAKMTGAPAKALMGPWEHRYAHISQIEAADFHGEVLRWFDHYLKGEDAGADALPDYRVFLQEFTEPSPKNLP
QNGRWVAEAHWPSPNVSDVTWPLARAGFAAAPQAGTVVVKSAATVGQAGGYFCPGIRIDNERPGDQSVDDAASTCFDSTP
LLAPMEILGRPRVRLAFSVDKPVAQVVARLCDVAPSGQSQRITYRPRNLCHDDSHEVATKLEPGKRYEVEFELNECAHRV
LAGHRLRLALSTSYWPIVWPAPEAAEIMLHLEDCALVLPERRVEVEIDAQAPGAARDYPRYQAQVLEKPWGEATTRIEAD
GTHVLESYDSYGAARDLSHGQQVSSNVRMRYAIHPDDPASARFDCAWVFAFGRETDGDAPDWNLRIETDSAMWCDTETFT
LWREIKAFEGEDLILTRDWKEVIPRGCH
>Mature_667_residues
TQPSAITVTEHLWIPLPDGRRLAARMWMPEGKGPFPVILEYLPYRKRDGTAPRDATTHPVFAAEGYACLRVDIAGSGDSD
GRFDDEYSEQELSDGEAVLEWIAAQPWSSGNVGMIGISWGGFNGLQMAYRRPEALKAVVSVASTVDRYADDIHYMGGCLL
SDNKNWASQMFAYMTRPLDPKLRPDWREEWLGRINDLPFMAADWLKHPTRDTFWKHGSVSEDWSAIKAPVLAITGWADAY
VNAPSALAAKMTGAPAKALMGPWEHRYAHISQIEAADFHGEVLRWFDHYLKGEDAGADALPDYRVFLQEFTEPSPKNLPQ
NGRWVAEAHWPSPNVSDVTWPLARAGFAAAPQAGTVVVKSAATVGQAGGYFCPGIRIDNERPGDQSVDDAASTCFDSTPL
LAPMEILGRPRVRLAFSVDKPVAQVVARLCDVAPSGQSQRITYRPRNLCHDDSHEVATKLEPGKRYEVEFELNECAHRVL
AGHRLRLALSTSYWPIVWPAPEAAEIMLHLEDCALVLPERRVEVEIDAQAPGAARDYPRYQAQVLEKPWGEATTRIEADG
THVLESYDSYGAARDLSHGQQVSSNVRMRYAIHPDDPASARFDCAWVFAFGRETDGDAPDWNLRIETDSAMWCDTETFTL
WREIKAFEGEDLILTRDWKEVIPRGCH

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 74563; Mature: 74432

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPSAITVTEHLWIPLPDGRRLAARMWMPEGKGPFPVILEYLPYRKRDGTAPRDATTHP
CCCCCEEEEEEEEEEECCCCCCEEHEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
VFAAEGYACLRVDIAGSGDSDGRFDDEYSEQELSDGEAVLEWIAAQPWSSGNVGMIGISW
EEEECCEEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEEC
GGFNGLQMAYRRPEALKAVVSVASTVDRYADDIHYMGGCLLSDNKNWASQMFAYMTRPLD
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCC
PKLRPDWREEWLGRINDLPFMAADWLKHPTRDTFWKHGSVSEDWSAIKAPVLAITGWADA
CCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCEEEEECCHHH
YVNAPSALAAKMTGAPAKALMGPWEHRYAHISQIEAADFHGEVLRWFDHYLKGEDAGADA
HCCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDYRVFLQEFTEPSPKNLPQNGRWVAEAHWPSPNVSDVTWPLARAGFAAAPQAGTVVVK
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCEEEEE
SAATVGQAGGYFCPGIRIDNERPGDQSVDDAASTCFDSTPLLAPMEILGRPRVRLAFSVD
CCHHHCCCCCEECCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEECC
KPVAQVVARLCDVAPSGQSQRITYRPRNLCHDDSHEVATKLEPGKRYEVEFELNECAHRV
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEHHHHHHHH
LAGHRLRLALSTSYWPIVWPAPEAAEIMLHLEDCALVLPERRVEVEIDAQAPGAARDYPR
HCCCEEEEEEECCCCEEEECCCCHHHEEEEECHHHEECCCCEEEEEEECCCCCCCCCCCH
YQAQVLEKPWGEATTRIEADGTHVLESYDSYGAARDLSHGQQVSSNVRMRYAIHPDDPAS
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCHHHHCCCHHHCCCCEEEEEECCCCCCC
ARFDCAWVFAFGRETDGDAPDWNLRIETDSAMWCDTETFTLWREIKAFEGEDLILTRDWK
CCEEEEEEEEECCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCCCCCEEEECCHH
EVIPRGCH
HHCCCCCC
>Mature Secondary Structure 
TQPSAITVTEHLWIPLPDGRRLAARMWMPEGKGPFPVILEYLPYRKRDGTAPRDATTHP
CCCCEEEEEEEEEEECCCCCCEEHEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
VFAAEGYACLRVDIAGSGDSDGRFDDEYSEQELSDGEAVLEWIAAQPWSSGNVGMIGISW
EEEECCEEEEEEEEECCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCCCEEEEEEEC
GGFNGLQMAYRRPEALKAVVSVASTVDRYADDIHYMGGCLLSDNKNWASQMFAYMTRPLD
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCC
PKLRPDWREEWLGRINDLPFMAADWLKHPTRDTFWKHGSVSEDWSAIKAPVLAITGWADA
CCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCHHHCCCCCCCCCHHHHCCCEEEEECCHHH
YVNAPSALAAKMTGAPAKALMGPWEHRYAHISQIEAADFHGEVLRWFDHYLKGEDAGADA
HCCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LPDYRVFLQEFTEPSPKNLPQNGRWVAEAHWPSPNVSDVTWPLARAGFAAAPQAGTVVVK
CCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCEEEEE
SAATVGQAGGYFCPGIRIDNERPGDQSVDDAASTCFDSTPLLAPMEILGRPRVRLAFSVD
CCHHHCCCCCEECCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCHHHHCCCCEEEEEECC
KPVAQVVARLCDVAPSGQSQRITYRPRNLCHDDSHEVATKLEPGKRYEVEFELNECAHRV
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCEEEEEEEHHHHHHHH
LAGHRLRLALSTSYWPIVWPAPEAAEIMLHLEDCALVLPERRVEVEIDAQAPGAARDYPR
HCCCEEEEEEECCCCEEEECCCCHHHEEEEECHHHEECCCCEEEEEEECCCCCCCCCCCH
YQAQVLEKPWGEATTRIEADGTHVLESYDSYGAARDLSHGQQVSSNVRMRYAIHPDDPAS
HHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCHHHHCCCHHHCCCCEEEEEECCCCCCC
ARFDCAWVFAFGRETDGDAPDWNLRIETDSAMWCDTETFTLWREIKAFEGEDLILTRDWK
CCEEEEEEEEECCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHHHHCCCCCEEEECCHH
EVIPRGCH
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]