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The map label for this gene is ureF

Identifier: 99080222

GI number: 99080222

Start: 390856

End: 391500

Strand: Reverse

Name: ureF

Synonym: TM1040_0381

Alternate gene names: 99080222

Gene position: 391500-390856 (Counterclockwise)

Preceding gene: 99080223

Following gene: 99080221

Centisome position: 12.23

GC content: 62.33

Gene sequence:

>645_bases
ATGACCAGTGAGGCGACGCTGAAGCTGATGCAATGGCTTTCGCCGGCCTATCCGGTGGGGGCTTTTGCCTATTCGCACGG
GCTCGAAGGCGCCGTCTCAGAGGGGCATGTGCGCGACGGGGACACATTGTCGGACTGGCTGAGCGACCTGCTGACCCATG
GTGGCGCGCGCTCGGATGCGCTGCTCTTGGCCTGTGCCTATCGCACAGAAACCGCAGAGTCGCTGGCGGAGATCGACGAC
ACCGCGCGCGCGTTTTGCCCCTCGGCGGAACGTCTGCAGGAGACCGACCTGCAGGGCGCGGCCTTTTGTCGGACCACGGC
CGCGATCTGGGACACAGCATTGCCTAGCCTCACCTACCCCGTGGCCGTGGGGCACGCGGCCAGACTCAATGATGTTGCGC
TGGATCTTACGCTGAACATGTATCTGCATGCCTTCCTCTCCAACCTTGTTGCGGCCGGTCAAAGGCTTCTTTCGCTTGGA
CAGACGGAGGCACAGCAACGCCTCAACGCCTGCGGGCCACAACTGCGCAGGACGGTCGACGCGGCGCTCTCGGGTACGAT
CGACGATCTCTTTGGTGCGAGTTTTGCCGCCGATATTGCCTCCATGCGCCATGAAACCCAATATTCGAGGATCTTTCGCT
CATGA

Upstream 100 bases:

>100_bases
TATGGTCACGGGCGCACCCACGCCCATGAACACGGCCATACGAACCATCACGGCCAGCACCACGATCACGCAGATCATGG
CCACAGCCACGACCATTCCC

Downstream 100 bases:

>100_bases
CATCGCAGACAACTGCGCCCCATGGACCGCTCCGCGTCGGCATCGGCGGCCCCGTGGGAGCAGGCAAGACCACGCTCATG
GCAGAGCTTGCCCGCGCGCT

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTSEATLKLMQWLSPAYPVGAFAYSHGLEGAVSEGHVRDGDTLSDWLSDLLTHGGARSDALLLACAYRTETAESLAEIDD
TARAFCPSAERLQETDLQGAAFCRTTAAIWDTALPSLTYPVAVGHAARLNDVALDLTLNMYLHAFLSNLVAAGQRLLSLG
QTEAQQRLNACGPQLRRTVDAALSGTIDDLFGASFAADIASMRHETQYSRIFRS

Sequences:

>Translated_214_residues
MTSEATLKLMQWLSPAYPVGAFAYSHGLEGAVSEGHVRDGDTLSDWLSDLLTHGGARSDALLLACAYRTETAESLAEIDD
TARAFCPSAERLQETDLQGAAFCRTTAAIWDTALPSLTYPVAVGHAARLNDVALDLTLNMYLHAFLSNLVAAGQRLLSLG
QTEAQQRLNACGPQLRRTVDAALSGTIDDLFGASFAADIASMRHETQYSRIFRS
>Mature_213_residues
TSEATLKLMQWLSPAYPVGAFAYSHGLEGAVSEGHVRDGDTLSDWLSDLLTHGGARSDALLLACAYRTETAESLAEIDDT
ARAFCPSAERLQETDLQGAAFCRTTAAIWDTALPSLTYPVAVGHAARLNDVALDLTLNMYLHAFLSNLVAAGQRLLSLGQ
TEAQQRLNACGPQLRRTVDAALSGTIDDLFGASFAADIASMRHETQYSRIFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_SILST (Q1GJQ2)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612376.1
- STRING:   Q1GJQ2
- GeneID:   4078614
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0381
- NMPDR:   fig|292414.1.peg.2443
- eggNOG:   COG0830
- HOGENOM:   HBG709295
- OMA:   WLSPAFP
- ProtClustDB:   CLSK759156
- BioCyc:   SSP292414:TM1040_0381-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 23039; Mature: 22908

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEATLKLMQWLSPAYPVGAFAYSHGLEGAVSEGHVRDGDTLSDWLSDLLTHGGARSDA
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
LLLACAYRTETAESLAEIDDTARAFCPSAERLQETDLQGAAFCRTTAAIWDTALPSLTYP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHH
VAVGHAARLNDVALDLTLNMYLHAFLSNLVAAGQRLLSLGQTEAQQRLNACGPQLRRTVD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AALSGTIDDLFGASFAADIASMRHETQYSRIFRS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSEATLKLMQWLSPAYPVGAFAYSHGLEGAVSEGHVRDGDTLSDWLSDLLTHGGARSDA
CCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCH
LLLACAYRTETAESLAEIDDTARAFCPSAERLQETDLQGAAFCRTTAAIWDTALPSLTYP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHH
VAVGHAARLNDVALDLTLNMYLHAFLSNLVAAGQRLLSLGQTEAQQRLNACGPQLRRTVD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
AALSGTIDDLFGASFAADIASMRHETQYSRIFRS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA