| Definition | Ruegeria sp. TM1040, complete genome. |
|---|---|
| Accession | NC_008044 |
| Length | 3,200,938 |
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The map label for this gene is tuf1 [H]
Identifier: 99080083
GI number: 99080083
Start: 262178
End: 263353
Strand: Direct
Name: tuf1 [H]
Synonym: TM1040_0242
Alternate gene names: 99080083
Gene position: 262178-263353 (Clockwise)
Preceding gene: 99080082
Following gene: 99080084
Centisome position: 8.19
GC content: 59.18
Gene sequence:
>1176_bases ATGGCTAAGGAAAAGTTTGAACGTAATAAACCGCACGTCAACATCGGCACCGTTGGCCACGTTGACCACGGTAAAACCAC GCTGACCGCAGCAATCACCAAATACTTCGGCGACTTCAAGGCATACGACCAGATCGATGGCGCGCCGGAAGAAAAAGCGC GTGGTATCACCATCTCCACCGCCCACGTGGAATACGAAACCGAAGGCCGTCACTACGCACACGTCGACTGCCCCGGCCAC GCTGACTATGTGAAAAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTGGTTGTGAACGCAGCCGACGGCCC GATGCCCCAGACCCGCGAGCACATCCTGCTGGCGCGTCAGGTTGGCGTTCCCGCGCTGGTCGTGTTCATGAACAAAGTGG ACCAGGTCGACGACGAAGAGCTGCTCGAGCTCGTCGAGATGGAAATCCGCGAATTGCTGTCGTCCTACGACTTCCCCGGC GACGATATCCCGATCATCGCAGGTTCCGCTCTGGCGGCGATGGAAGGCCGTGACCCGGAAATCGGCGAAAACAAGATCAA GGAACTGATGGCGGCTGTGGATGAGTACATCCCGACCCCCGAGCGCGCTGTTGACCAGCCGTTCCTGATGCCGATCGAAG ACGTGTTCTCGATCTCTGGCCGTGGTACCGTTGTGACCGGTCGTGTTGAGCGTGGCGTGATCAATGTTGGCGACAACATC GAAATCGTTGGCATCAAAGACACCACCACCACCACCTGTACCGGTGTGGAAATGTTCCGCAAGCTGCTGGACCGCGGTGA AGCAGGCGACAACATCGGCGCCCTGCTGCGTGGTATCGACCGTGAAGCGGTTGAGCGTGGCCAGGTTCTGTGTAAGCCGG GCTCCGTGACCCCGCACACCAAATTCGAGGCAGAAGCTTATATTCTGACCAAAGAAGAAGGTGGCCGTCACACCCCGTTC TTTGCGAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGCACCGTGACCCTGCCGGAAGGCACCGAAAT GGTGATGCCGGGCGACAACCTGAAGTTCGAAGTTGAACTGATCGCCCCGATCGCAATGGAAGACGGTCTGCGTTTCGCGA TCCGCGAAGGCGGCCGCACCGTTGGCGCGGGCGTTGTGTCCAAGATCCTCGCGTAA
Upstream 100 bases:
>100_bases AGAACATCTCTGAAGAGATCCAGGCGAAATACGCATAAGCGTAACGATTTGGGGGAGTGCCGCCGCCGGCGCTCCCTCCC AAAGCTAAAAGGAGGCCATC
Downstream 100 bases:
>100_bases GCGAAGATCCTCGCCTGATTTAAATGGCGAGTGACAATAGAGAGGGCCTCCCAATGGGAGGCCCTTTTTTGTTGCGAGGC GCTGCCTCGCGCTCCGAGGT
Product: elongation factor Tu
Products: NA
Alternate protein names: EF-Tu [H]
Number of amino acids: Translated: 391; Mature: 390
Protein sequence:
>391_residues MAKEKFERNKPHVNIGTVGHVDHGKTTLTAAITKYFGDFKAYDQIDGAPEEKARGITISTAHVEYETEGRHYAHVDCPGH ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVFMNKVDQVDDEELLELVEMEIRELLSSYDFPG DDIPIIAGSALAAMEGRDPEIGENKIKELMAAVDEYIPTPERAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDNI EIVGIKDTTTTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVTPHTKFEAEAYILTKEEGGRHTPF FANYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVSKILA
Sequences:
>Translated_391_residues MAKEKFERNKPHVNIGTVGHVDHGKTTLTAAITKYFGDFKAYDQIDGAPEEKARGITISTAHVEYETEGRHYAHVDCPGH ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVFMNKVDQVDDEELLELVEMEIRELLSSYDFPG DDIPIIAGSALAAMEGRDPEIGENKIKELMAAVDEYIPTPERAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDNI EIVGIKDTTTTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVTPHTKFEAEAYILTKEEGGRHTPF FANYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVSKILA >Mature_390_residues AKEKFERNKPHVNIGTVGHVDHGKTTLTAAITKYFGDFKAYDQIDGAPEEKARGITISTAHVEYETEGRHYAHVDCPGHA DYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQVGVPALVVFMNKVDQVDDEELLELVEMEIRELLSSYDFPGD DIPIIAGSALAAMEGRDPEIGENKIKELMAAVDEYIPTPERAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDNIE IVGIKDTTTTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVTPHTKFEAEAYILTKEEGGRHTPFF ANYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVSKILA
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG0050
COG function: function code J; GTPases - translation elongation factors
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.7215189873418, Blast_Score=493, Evalue=1e-139, Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=30.6306306306306, Blast_Score=173, Evalue=2e-43, Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=30.6306306306306, Blast_Score=173, Evalue=2e-43, Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=27.4553571428571, Blast_Score=148, Evalue=1e-35, Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=27.9017857142857, Blast_Score=146, Evalue=4e-35, Organism=Homo sapiens, GI194097354, Length=347, Percent_Identity=29.971181556196, Blast_Score=122, Evalue=6e-28, Organism=Homo sapiens, GI194018522, Length=347, Percent_Identity=29.971181556196, Blast_Score=122, Evalue=6e-28, Organism=Homo sapiens, GI194018520, Length=347, Percent_Identity=29.971181556196, Blast_Score=122, Evalue=7e-28, Organism=Homo sapiens, GI46094014, Length=341, Percent_Identity=30.791788856305, Blast_Score=121, Evalue=1e-27, Organism=Homo sapiens, GI54607086, Length=317, Percent_Identity=29.6529968454259, Blast_Score=120, Evalue=2e-27, Organism=Homo sapiens, GI157426893, Length=302, Percent_Identity=27.1523178807947, Blast_Score=79, Evalue=5e-15, Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=27.1062271062271, Blast_Score=78, Evalue=2e-14, Organism=Homo sapiens, GI94966754, Length=143, Percent_Identity=31.4685314685315, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI25306287, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=8e-11, Organism=Homo sapiens, GI25306283, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=9e-11, Organism=Homo sapiens, GI19923640, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=9e-11, Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=77.020202020202, Blast_Score=597, Evalue=1e-172, Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=77.020202020202, Blast_Score=596, Evalue=1e-172, Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=31.5508021390374, Blast_Score=145, Evalue=4e-36, Organism=Escherichia coli, GI48994988, Length=269, Percent_Identity=31.5985130111524, Blast_Score=108, Evalue=6e-25, Organism=Escherichia coli, GI1789108, Length=263, Percent_Identity=31.1787072243346, Blast_Score=91, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.8847117794486, Blast_Score=412, Evalue=1e-115, Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.4108527131783, Blast_Score=320, Evalue=7e-88, Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=28.6681715575621, Blast_Score=152, Evalue=2e-37, Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=28.6681715575621, Blast_Score=152, Evalue=2e-37, Organism=Caenorhabditis elegans, GI115532067, Length=443, Percent_Identity=28.2167042889391, Blast_Score=134, Evalue=9e-32, Organism=Caenorhabditis elegans, GI115532065, Length=443, Percent_Identity=28.2167042889391, Blast_Score=134, Evalue=1e-31, Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=27.4418604651163, Blast_Score=130, Evalue=9e-31, Organism=Caenorhabditis elegans, GI32566629, Length=350, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=9e-29, Organism=Caenorhabditis elegans, GI17506081, Length=295, Percent_Identity=29.4915254237288, Blast_Score=117, Evalue=8e-27, Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=34.9397590361446, Blast_Score=90, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=9e-15, Organism=Caenorhabditis elegans, GI17509919, Length=305, Percent_Identity=27.2131147540984, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17556745, Length=130, Percent_Identity=30, Blast_Score=65, Evalue=6e-11, Organism=Caenorhabditis elegans, GI17533571, Length=135, Percent_Identity=31.8518518518519, Blast_Score=64, Evalue=1e-10, Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=66.3291139240506, Blast_Score=528, Evalue=1e-151, Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=28.6363636363636, Blast_Score=150, Evalue=5e-37, Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=28.6363636363636, Blast_Score=150, Evalue=5e-37, Organism=Saccharomyces cerevisiae, GI6322937, Length=273, Percent_Identity=28.2051282051282, Blast_Score=95, Evalue=2e-20, Organism=Saccharomyces cerevisiae, GI6320377, Length=341, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=3e-17, Organism=Saccharomyces cerevisiae, GI6320863, Length=273, Percent_Identity=27.4725274725275, Blast_Score=79, Evalue=9e-16, Organism=Saccharomyces cerevisiae, GI6323098, Length=177, Percent_Identity=31.0734463276836, Blast_Score=72, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6323320, Length=260, Percent_Identity=26.5384615384615, Blast_Score=70, Evalue=5e-13, Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=31.2977099236641, Blast_Score=68, Evalue=2e-12, Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=57.7608142493639, Blast_Score=478, Evalue=1e-135, Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=57.7608142493639, Blast_Score=478, Evalue=1e-135, Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=49.4845360824742, Blast_Score=396, Evalue=1e-110, Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36, Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36, Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36, Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=2e-36, Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=28.1318681318681, Blast_Score=146, Evalue=2e-35, Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=28.1318681318681, Blast_Score=146, Evalue=2e-35, Organism=Drosophila melanogaster, GI45550900, Length=346, Percent_Identity=28.3236994219653, Blast_Score=132, Evalue=3e-31, Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=30.3333333333333, Blast_Score=130, Evalue=2e-30, Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=30.7471264367816, Blast_Score=125, Evalue=7e-29, Organism=Drosophila melanogaster, GI24647048, Length=268, Percent_Identity=26.1194029850746, Blast_Score=77, Evalue=2e-14, Organism=Drosophila melanogaster, GI24647046, Length=268, Percent_Identity=26.1194029850746, Blast_Score=77, Evalue=2e-14, Organism=Drosophila melanogaster, GI28572034, Length=159, Percent_Identity=35.8490566037736, Blast_Score=69, Evalue=7e-12, Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=4e-11, Organism=Drosophila melanogaster, GI78706572, Length=254, Percent_Identity=25.9842519685039, Blast_Score=66, Evalue=4e-11,
Paralogues:
None
Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004161 - InterPro: IPR004541 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: NA
Molecular weight: Translated: 42705; Mature: 42574
Theoretical pI: Translated: 4.61; Mature: 4.61
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKEKFERNKPHVNIGTVGHVDHGKTTLTAAITKYFGDFKAYDQIDGAPEEKARGITIST CCCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHCCEEEEE AHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQ EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH VGVPALVVFMNKVDQVDDEELLELVEMEIRELLSSYDFPGDDIPIIAGSALAAMEGRDPE CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEECCCCCCC IGENKIKELMAAVDEYIPTPERAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDNI CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCCE EIVGIKDTTTTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVTPHT EEEEECCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC KFEAEAYILTKEEGGRHTPFFANYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNLKFEVEL CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEEEEE IAPIAMEDGLRFAIREGGRTVGAGVVSKILA EEEEECCCCCEEEECCCCCEEHHHHHHHHCC >Mature Secondary Structure AKEKFERNKPHVNIGTVGHVDHGKTTLTAAITKYFGDFKAYDQIDGAPEEKARGITIST CCCHHHCCCCEEEECEEECCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHCCEEEEE AHVEYETEGRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLARQ EEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH VGVPALVVFMNKVDQVDDEELLELVEMEIRELLSSYDFPGDDIPIIAGSALAAMEGRDPE CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEECCCCCCC IGENKIKELMAAVDEYIPTPERAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDNI CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCCE EIVGIKDTTTTTCTGVEMFRKLLDRGEAGDNIGALLRGIDREAVERGQVLCKPGSVTPHT EEEEECCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCCEEECCCCCCCCC KFEAEAYILTKEEGGRHTPFFANYRPQFYFRTTDVTGTVTLPEGTEMVMPGDNLKFEVEL CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEEEEE IAPIAMEDGLRFAIREGGRTVGAGVVSKILA EEEEECCCCCEEEECCCCCEEHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA