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The map label for this gene is rihC [H]

Identifier: 99080066

GI number: 99080066

Start: 240406

End: 241329

Strand: Direct

Name: rihC [H]

Synonym: TM1040_0225

Alternate gene names: 99080066

Gene position: 240406-241329 (Clockwise)

Preceding gene: 99080065

Following gene: 99080067

Centisome position: 7.51

GC content: 60.5

Gene sequence:

>924_bases
ATGACAACAAAACTCATCATCGACACGGACCCCGGCATCGACGATGCGATGGCTATTTTCTACGCCGCCGCCGCGCCCGA
TATCGAGCTTCTGGGGCTGACCACGATCTTTGGCAATGTGACCACCAAGATGGCCACCCGCAATGCGCTGCGTCTGCTTG
AGGCCGCTGAACTGGATCTGCCCGTTGCCCATGGCGCGGAAAAGCCGCTGGTCCTGCCCCCCTTTGAGCCTTCGGCCCAT
GTGCATGGCGACGAAGGCTTTGGCGACATTCCCGCCGTCAATCCCAAAGGGCAGGCGATTGCAGAAGATGCCGCCGATTT
CCTGATCCGAATGGCGCGCGAACACAAGGGCGAGCTGGTGGTCTGCCCGGTGGGGCCGCTGACGAATATCGCCATCGCGA
TCGAGCGTGACCCGGAGTTTGTGAAAAACTGCAAGCGCATCGTCATCATGGGGGGCTCGCTTGAGGCGGGCGGAAATATC
ACGCCCCATGCCGAGGCCAATATCTACCACGACCCCCACGCCGCCGAGGCGGTCTTTGCCGCCGCCGCGGGCAAGGTGGT
GATGGTTGGGCTTGATGTGACGCTCAAGATTCTCTGCACTCCCGAGGATTTCGACGGCATCAAGGAGCGCTCGCCGGAGC
TTGGTGGCATGCTCAAGGACATGAGTGTCTTTTACATCAAGTTCTATCAGGAAGTGGCCAATCTCAATGGATGTTCTCTG
CATGACCCCGCAGCGGTGATTGCCTGCACCCATCCCGACCTCTTTAAGACCCGGGCCGTGAAACTGGCCGTTTCGGTCGA
GGAGGAGACCTCTGGCAAGACCCAGCTGGCCGAGGATGGTCGTGGCGATACACATGTCGCGATGGACGTGGACGCAGAGG
CCGTCAAGGCACTGTTCCTGAAGCGCCTATCGCTGCTGCCCTGA

Upstream 100 bases:

>100_bases
TTTTGCGAAACAGTTGATGGATAATCGAGAGGCTGGCAGGCTTGAGTTGCCGCTAACATAGGTGCTAAACCCGCTCGATT
AATCAGTCTTGGGGATAACA

Downstream 100 bases:

>100_bases
ACCACTCCGCCTGTGCCGGTGTGGCGCGGGCGGATCGGGCGAGAGGGCCAAGTCGCCCTCTTGCCGCCACGTCCAAAACG
ACGAATTCAGCCGGAGGGTG

Product: inosine/uridine-preferring nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTTKLIIDTDPGIDDAMAIFYAAAAPDIELLGLTTIFGNVTTKMATRNALRLLEAAELDLPVAHGAEKPLVLPPFEPSAH
VHGDEGFGDIPAVNPKGQAIAEDAADFLIRMAREHKGELVVCPVGPLTNIAIAIERDPEFVKNCKRIVIMGGSLEAGGNI
TPHAEANIYHDPHAAEAVFAAAAGKVVMVGLDVTLKILCTPEDFDGIKERSPELGGMLKDMSVFYIKFYQEVANLNGCSL
HDPAAVIACTHPDLFKTRAVKLAVSVEEETSGKTQLAEDGRGDTHVAMDVDAEAVKALFLKRLSLLP

Sequences:

>Translated_307_residues
MTTKLIIDTDPGIDDAMAIFYAAAAPDIELLGLTTIFGNVTTKMATRNALRLLEAAELDLPVAHGAEKPLVLPPFEPSAH
VHGDEGFGDIPAVNPKGQAIAEDAADFLIRMAREHKGELVVCPVGPLTNIAIAIERDPEFVKNCKRIVIMGGSLEAGGNI
TPHAEANIYHDPHAAEAVFAAAAGKVVMVGLDVTLKILCTPEDFDGIKERSPELGGMLKDMSVFYIKFYQEVANLNGCSL
HDPAAVIACTHPDLFKTRAVKLAVSVEEETSGKTQLAEDGRGDTHVAMDVDAEAVKALFLKRLSLLP
>Mature_306_residues
TTKLIIDTDPGIDDAMAIFYAAAAPDIELLGLTTIFGNVTTKMATRNALRLLEAAELDLPVAHGAEKPLVLPPFEPSAHV
HGDEGFGDIPAVNPKGQAIAEDAADFLIRMAREHKGELVVCPVGPLTNIAIAIERDPEFVKNCKRIVIMGGSLEAGGNIT
PHAEANIYHDPHAAEAVFAAAAGKVVMVGLDVTLKILCTPEDFDGIKERSPELGGMLKDMSVFYIKFYQEVANLNGCSLH
DPAAVIACTHPDLFKTRAVKLAVSVEEETSGKTQLAEDGRGDTHVAMDVDAEAVKALFLKRLSLLP

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=301, Percent_Identity=33.5548172757475, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786213, Length=312, Percent_Identity=36.8589743589744, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786871, Length=315, Percent_Identity=33.968253968254, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=36.3157894736842, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=185, Percent_Identity=37.2972972972973, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320608, Length=255, Percent_Identity=30.5882352941176, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641839, Length=185, Percent_Identity=32.972972972973, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24641837, Length=210, Percent_Identity=31.4285714285714, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24583915, Length=189, Percent_Identity=30.1587301587302, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32754; Mature: 32623

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKLIIDTDPGIDDAMAIFYAAAAPDIELLGLTTIFGNVTTKMATRNALRLLEAAELDL
CCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCC
PVAHGAEKPLVLPPFEPSAHVHGDEGFGDIPAVNPKGQAIAEDAADFLIRMAREHKGELV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VCPVGPLTNIAIAIERDPEFVKNCKRIVIMGGSLEAGGNITPHAEANIYHDPHAAEAVFA
EECCCCCCEEEEEEECCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHH
AAAGKVVMVGLDVTLKILCTPEDFDGIKERSPELGGMLKDMSVFYIKFYQEVANLNGCSL
HCCCCEEEEEECEEEEEEECCCCCCCHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCC
HDPAAVIACTHPDLFKTRAVKLAVSVEEETSGKTQLAEDGRGDTHVAMDVDAEAVKALFL
CCCCEEEEECCCCHHHHEEEEEEEEECCCCCCCCCHHCCCCCCEEEEEECCHHHHHHHHH
KRLSLLP
HHHHCCC
>Mature Secondary Structure 
TTKLIIDTDPGIDDAMAIFYAAAAPDIELLGLTTIFGNVTTKMATRNALRLLEAAELDL
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCC
PVAHGAEKPLVLPPFEPSAHVHGDEGFGDIPAVNPKGQAIAEDAADFLIRMAREHKGELV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
VCPVGPLTNIAIAIERDPEFVKNCKRIVIMGGSLEAGGNITPHAEANIYHDPHAAEAVFA
EECCCCCCEEEEEEECCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHH
AAAGKVVMVGLDVTLKILCTPEDFDGIKERSPELGGMLKDMSVFYIKFYQEVANLNGCSL
HCCCCEEEEEECEEEEEEECCCCCCCHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCC
HDPAAVIACTHPDLFKTRAVKLAVSVEEETSGKTQLAEDGRGDTHVAMDVDAEAVKALFL
CCCCEEEEECCCCHHHHEEEEEEEEECCCCCCCCCHHCCCCCCEEEEEECCHHHHHHHHH
KRLSLLP
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA