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The map label for this gene is metG

Identifier: 161899027

GI number: 161899027

Start: 2307335

End: 2309056

Strand: Direct

Name: metG

Synonym: TM1040_2197

Alternate gene names: 161899027

Gene position: 2307335-2309056 (Clockwise)

Preceding gene: 99082034

Following gene: 99082038

Centisome position: 72.08

GC content: 61.03

Gene sequence:

>1722_bases
ATGGCCCGAATTCTCATCACCTCGGCACTGCCCTATATCAATGGGATCAAGCACCTCGGCAACCTCGTGGGGAGCCAACT
TCCTGCCGATCTCTATGCCCGCTACAACCGGGGACGCGGCCATGAGGTCATGTTCCTCTGCGCGACCGACGAACACGGCA
CCCCCGCAGAACTGGCCGCAGCCAAAGCCGGCAAGCCCGTGGCCGATTATTGCGCCGAAATGCACGCCGTGCAGGCCGAC
ATCGCCAAGCGGTTTGGCCTGTCGTTTGACCATTATGGCCGCTCCTCGAGCCCGCAGAACCACGCGCTGACTCAGCATTT
TGCGGGCAAGCTCGAAGAGGCTGGCCTGATTACCGAAGTGTCGGAAAAACAGGTCTATTCCAACGCCGATGGCCGCTTTC
TGCCTGATCGCTATATCGAGGGCACCTGCCCCAACTGCGGCTTTGAGAAGGCGCGCGGCGATCAATGCGAGGAATGCACC
AAACAGCTCGATCCGACCGACCTCATCAACCCGCGCTCTGCGATCTCGGGGTCGACCGACCTTGAGGTGCGCGAGACCAA
GCACCTGTTCCTGCGCCAGTCCGCAATGCGCGACCAACTGGACCAGTGGATAGACTCTAAGACCGACTGGCCGGTGCTCA
CCACCTCGATTGCCAAGAAATGGCTGCATGATGGCGACGGTTTGCAGGACCGGGGCATCACCCGTGACCTCGACTGGGGG
ATTGCGGTCAAAAAAGGCGACGCGGACTGGCCTGGCATGGAAGGCAAGGTCTTCTACGTCTGGTTCGACGCACCGATTGA
ATATATCGCAGCCGCTGGCGAATGGGCCGAGGCCCATGGCAAAACCGATGCCGATTGGGAGCGCTGGTGGCGCACCGACA
AGGGCGCGGATGACGTCAAATATGTGCAGTTCATGGGCAAGGACAACGTTCCCTTCCACACCCTGTCCTTCCCCGCGACC
CTGCTGGGGTCGGGCGAGCCGTGGAAGATGGTCGATCATCTGAAATCCTTCAACTACCTCAACTATGATGGCGGTCAGTT
CTCCACCTCGCAGGGGCGCGGCGTGTTCATGGATCAGGCGCTCGAGATCCTGCCTGCCGATTACTGGCGCTGGTGGCTGC
TGTCCCATGCCCCCGAAAGCTCCGATTCCGAATTCACCTGGGAGAACTTCCAGCAATCGGTGAACAAGGATCTGGCGGAT
GTGCTTGGGAACTTTGTCTCGCGCATCACCAAATTCTGCCGCTCGAAATTTGGCGAAGAGGTGCCCGATGGCGGCACCTG
GGGCGAACAGGAACAGACCCTCATTGAAGAGCTCTCCACCCGCATCGGCGCCTTTGAACGCCATATGGAGGCGATGGAGG
TGCGCAAATCGGCGCAGGAACTCCGCGCGATCTGGGTTGCGGGCAATGAATACCTGCAATCTGCCGCGCCTTGGTCGGTG
TTCAAGGAAGACCCCGAGCGCGCGGCAGGTCAGGTCCGCCTCGGCCTCAATCTGATCCGTCTCTATGCGGTTCTGTCGGC
GCCCTTCATTCCAGATGCCTCCGATCGCCTGTTTGCCGCTCTCGGCACCGAGGATCGCAGCTGGCCCGAAGACGTTGCCG
CCGCACTCGCCGCCTTGCCCGCAGGCCATGCCTTCACGGTGCCGGAGGTGCTCTTTGCCAAGATTACCGATGAGCAGCGC
GAAGAGTGGCAAGAGCGCTTTGCCGGAGGTCGCAGCGCCTAA

Upstream 100 bases:

>100_bases
GCGTTTCACCGGTTTCTTTGCGTTGCACTGGATCATGGGGTTTTCGTACCTTCCCGCATGGGATAGACGGGCTGCCAGAC
AAGCGACGTGGATGGACGAT

Downstream 100 bases:

>100_bases
GGCGCGCACCGCACGCTCCACCGCATCAAAGGCCGCCCCACCCGGGCGGCCTTTTTCATTGCCCATCCCCCATTCGCAAC
TTTGGAAAAATCAAAGTTTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MARILITSALPYINGIKHLGNLVGSQLPADLYARYNRGRGHEVMFLCATDEHGTPAELAAAKAGKPVADYCAEMHAVQAD
IAKRFGLSFDHYGRSSSPQNHALTQHFAGKLEEAGLITEVSEKQVYSNADGRFLPDRYIEGTCPNCGFEKARGDQCEECT
KQLDPTDLINPRSAISGSTDLEVRETKHLFLRQSAMRDQLDQWIDSKTDWPVLTTSIAKKWLHDGDGLQDRGITRDLDWG
IAVKKGDADWPGMEGKVFYVWFDAPIEYIAAAGEWAEAHGKTDADWERWWRTDKGADDVKYVQFMGKDNVPFHTLSFPAT
LLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFMDQALEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLAD
VLGNFVSRITKFCRSKFGEEVPDGGTWGEQEQTLIEELSTRIGAFERHMEAMEVRKSAQELRAIWVAGNEYLQSAAPWSV
FKEDPERAAGQVRLGLNLIRLYAVLSAPFIPDASDRLFAALGTEDRSWPEDVAAALAALPAGHAFTVPEVLFAKITDEQR
EEWQERFAGGRSA

Sequences:

>Translated_573_residues
MARILITSALPYINGIKHLGNLVGSQLPADLYARYNRGRGHEVMFLCATDEHGTPAELAAAKAGKPVADYCAEMHAVQAD
IAKRFGLSFDHYGRSSSPQNHALTQHFAGKLEEAGLITEVSEKQVYSNADGRFLPDRYIEGTCPNCGFEKARGDQCEECT
KQLDPTDLINPRSAISGSTDLEVRETKHLFLRQSAMRDQLDQWIDSKTDWPVLTTSIAKKWLHDGDGLQDRGITRDLDWG
IAVKKGDADWPGMEGKVFYVWFDAPIEYIAAAGEWAEAHGKTDADWERWWRTDKGADDVKYVQFMGKDNVPFHTLSFPAT
LLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFMDQALEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLAD
VLGNFVSRITKFCRSKFGEEVPDGGTWGEQEQTLIEELSTRIGAFERHMEAMEVRKSAQELRAIWVAGNEYLQSAAPWSV
FKEDPERAAGQVRLGLNLIRLYAVLSAPFIPDASDRLFAALGTEDRSWPEDVAAALAALPAGHAFTVPEVLFAKITDEQR
EEWQERFAGGRSA
>Mature_572_residues
ARILITSALPYINGIKHLGNLVGSQLPADLYARYNRGRGHEVMFLCATDEHGTPAELAAAKAGKPVADYCAEMHAVQADI
AKRFGLSFDHYGRSSSPQNHALTQHFAGKLEEAGLITEVSEKQVYSNADGRFLPDRYIEGTCPNCGFEKARGDQCEECTK
QLDPTDLINPRSAISGSTDLEVRETKHLFLRQSAMRDQLDQWIDSKTDWPVLTTSIAKKWLHDGDGLQDRGITRDLDWGI
AVKKGDADWPGMEGKVFYVWFDAPIEYIAAAGEWAEAHGKTDADWERWWRTDKGADDVKYVQFMGKDNVPFHTLSFPATL
LGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFMDQALEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLADV
LGNFVSRITKFCRSKFGEEVPDGGTWGEQEQTLIEELSTRIGAFERHMEAMEVRKSAQELRAIWVAGNEYLQSAAPWSVF
KEDPERAAGQVRLGLNLIRLYAVLSAPFIPDASDRLFAALGTEDRSWPEDVAAALAALPAGHAFTVPEVLFAKITDEQRE
EWQERFAGGRSA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=576, Percent_Identity=36.9791666666667, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI24308436, Length=550, Percent_Identity=25.0909090909091, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788432, Length=531, Percent_Identity=32.5800376647834, Blast_Score=259, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17540794, Length=581, Percent_Identity=34.5955249569707, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI212645096, Length=540, Percent_Identity=23.7037037037037, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71992781, Length=433, Percent_Identity=23.094688221709, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321704, Length=579, Percent_Identity=35.5785837651123, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655311, Length=576, Percent_Identity=36.6319444444444, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI21358483, Length=544, Percent_Identity=24.4485294117647, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_SILST (Q1GEI7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614191.2
- STRING:   Q1GEI7
- GeneID:   4078188
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2197
- NMPDR:   fig|292414.1.peg.566
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- PhylomeDB:   Q1GEI7
- ProtClustDB:   PRK12268
- BioCyc:   SSP292414:TM1040_2197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 64246; Mature: 64115

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 349-349

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILITSALPYINGIKHLGNLVGSQLPADLYARYNRGRGHEVMFLCATDEHGTPAELAA
CCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHH
AKAGKPVADYCAEMHAVQADIAKRFGLSFDHYGRSSSPQNHALTQHFAGKLEEAGLITEV
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
SEKQVYSNADGRFLPDRYIEGTCPNCGFEKARGDQCEECTKQLDPTDLINPRSAISGSTD
HHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCC
LEVRETKHLFLRQSAMRDQLDQWIDSKTDWPVLTTSIAKKWLHDGDGLQDRGITRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IAVKKGDADWPGMEGKVFYVWFDAPIEYIAAAGEWAEAHGKTDADWERWWRTDKGADDVK
EEEECCCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHCCCCCCCHHHHHCCCCCCCHHH
YVQFMGKDNVPFHTLSFPATLLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFMDQA
HHHHHCCCCCCEEEECCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHH
LEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLADVLGNFVSRITKFCRSKFGEE
HHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPDGGTWGEQEQTLIEELSTRIGAFERHMEAMEVRKSAQELRAIWVAGNEYLQSAAPWSV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCHHH
FKEDPERAAGQVRLGLNLIRLYAVLSAPFIPDASDRLFAALGTEDRSWPEDVAAALAALP
HHCCHHHHHCEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
AGHAFTVPEVLFAKITDEQREEWQERFAGGRSA
CCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARILITSALPYINGIKHLGNLVGSQLPADLYARYNRGRGHEVMFLCATDEHGTPAELAA
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHH
AKAGKPVADYCAEMHAVQADIAKRFGLSFDHYGRSSSPQNHALTQHFAGKLEEAGLITEV
HHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHH
SEKQVYSNADGRFLPDRYIEGTCPNCGFEKARGDQCEECTKQLDPTDLINPRSAISGSTD
HHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCC
LEVRETKHLFLRQSAMRDQLDQWIDSKTDWPVLTTSIAKKWLHDGDGLQDRGITRDLDWG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
IAVKKGDADWPGMEGKVFYVWFDAPIEYIAAAGEWAEAHGKTDADWERWWRTDKGADDVK
EEEECCCCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHCCCCCCCHHHHHCCCCCCCHHH
YVQFMGKDNVPFHTLSFPATLLGSGEPWKMVDHLKSFNYLNYDGGQFSTSQGRGVFMDQA
HHHHHCCCCCCEEEECCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHH
LEILPADYWRWWLLSHAPESSDSEFTWENFQQSVNKDLADVLGNFVSRITKFCRSKFGEE
HHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VPDGGTWGEQEQTLIEELSTRIGAFERHMEAMEVRKSAQELRAIWVAGNEYLQSAAPWSV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHCCCCHHH
FKEDPERAAGQVRLGLNLIRLYAVLSAPFIPDASDRLFAALGTEDRSWPEDVAAALAALP
HHCCHHHHHCEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
AGHAFTVPEVLFAKITDEQREEWQERFAGGRSA
CCCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA