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The map label for this gene is gcvA [H]

Identifier: 104779424

GI number: 104779424

Start: 111171

End: 112070

Strand: Reverse

Name: gcvA [H]

Synonym: PSEEN0118

Alternate gene names: 104779424

Gene position: 112070-111171 (Counterclockwise)

Preceding gene: 104779427

Following gene: 104779423

Centisome position: 1.9

GC content: 66.78

Gene sequence:

>900_bases
ATGCGCCGCAAGATCCCCAGCACCACGGCCCTGGTCTGCTTCGAAGCGGCCGCCCGGCACGAGAGTTTCACCAAGGCCGC
CCAGGAGCTGGCCCTGACCCAGGGCGCCGTCTGTCGGCAGATCGGTGGGCTGGAAGCCTTCCTCAATGTGGAACTGTTCC
GTCGCTCGCGGCGGGGCGTGAAGTTGACTGAGGCCGGGTTGTCCTACAGTCGCCAGGTGGCCTCGCAGCTGGACGCGGTG
GAGCGCGACACGCTGTCGGTGATGCGCCAGCAAGGCGCTAACGTGATCGAGCTGGCGGTCGTGCCGACGTTCGGCACCCA
GTGGCTGCTGCCTCGCCTCAAGGACTTCCAGCAACGCCACCCGGAAGTCACGGTCAACCTTACCAACCGCACCCGCCCGT
TCCTGTTCGCCGACACGCCCTTTGACGCCGCCATCTACTTCGGTGACGCCGACTGGTCGGGTACCCAGGCGCACCGGCTG
ATGGGCGAGAACCCGATGCCCGTGTGCAGCCCGGCATTGCTGGGCGGGCAGGGCAGCCTCGACGCGGGGCGGATCGCCGA
ACTGCCCCTGCTGCAGCAGACCACACGGCCCTACGCCTGGCGGCAGTGGTTCGACGGTCTCGGCATGAGCGTGGAGCGCG
ACATGACCGGCCCGCGCTATGAACTATTCTCCATGCTGGCCCAGGCGGCGATGCACGAGATGGGGATCGCGCTGATCCCG
CCGTTCCTGATCCAGCGCGAGTTGGAGGAGGGCAGGCTGGTGGTCGCTAACAGGCATGCCCTGGCCAGCGACAAGGCCTA
CCATCTGATGATCCCCGAGCGAAAGGTCGAATCTGCGTCATTGCGGGCCTTCCGCGACTGGCTGGTGGCCCAGGCCCAGC
AATATACGGCGAAGAACTAG

Upstream 100 bases:

>100_bases
CGTCGGGACAAACGAGGATTGCGCACGCTTTAGTGATATTTTCTCACTCCGTGATCAGTCAAAAGCCGTATTACACGACG
AATAAGTGAGGTTGACGTAC

Downstream 100 bases:

>100_bases
CGGGAAACATGGAAGCTGACCCCGTAGTCAATTATTTTAAACACCTACAGATATATGGATTTGTCGCAAATCCGTAATCT
GTTCCACTGTGCGGAAAAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRRKIPSTTALVCFEAAARHESFTKAAQELALTQGAVCRQIGGLEAFLNVELFRRSRRGVKLTEAGLSYSRQVASQLDAV
ERDTLSVMRQQGANVIELAVVPTFGTQWLLPRLKDFQQRHPEVTVNLTNRTRPFLFADTPFDAAIYFGDADWSGTQAHRL
MGENPMPVCSPALLGGQGSLDAGRIAELPLLQQTTRPYAWRQWFDGLGMSVERDMTGPRYELFSMLAQAAMHEMGIALIP
PFLIQRELEEGRLVVANRHALASDKAYHLMIPERKVESASLRAFRDWLVAQAQQYTAKN

Sequences:

>Translated_299_residues
MRRKIPSTTALVCFEAAARHESFTKAAQELALTQGAVCRQIGGLEAFLNVELFRRSRRGVKLTEAGLSYSRQVASQLDAV
ERDTLSVMRQQGANVIELAVVPTFGTQWLLPRLKDFQQRHPEVTVNLTNRTRPFLFADTPFDAAIYFGDADWSGTQAHRL
MGENPMPVCSPALLGGQGSLDAGRIAELPLLQQTTRPYAWRQWFDGLGMSVERDMTGPRYELFSMLAQAAMHEMGIALIP
PFLIQRELEEGRLVVANRHALASDKAYHLMIPERKVESASLRAFRDWLVAQAQQYTAKN
>Mature_299_residues
MRRKIPSTTALVCFEAAARHESFTKAAQELALTQGAVCRQIGGLEAFLNVELFRRSRRGVKLTEAGLSYSRQVASQLDAV
ERDTLSVMRQQGANVIELAVVPTFGTQWLLPRLKDFQQRHPEVTVNLTNRTRPFLFADTPFDAAIYFGDADWSGTQAHRL
MGENPMPVCSPALLGGQGSLDAGRIAELPLLQQTTRPYAWRQWFDGLGMSVERDMTGPRYELFSMLAQAAMHEMGIALIP
PFLIQRELEEGRLVVANRHALASDKAYHLMIPERKVESASLRAFRDWLVAQAQQYTAKN

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=35.2542372881356, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=28.9752650176678, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788706, Length=248, Percent_Identity=30.241935483871, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693193, Length=307, Percent_Identity=28.3387622149837, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=37.6068376068376, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33518; Mature: 33518

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIPSTTALVCFEAAARHESFTKAAQELALTQGAVCRQIGGLEAFLNVELFRRSRRGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
KLTEAGLSYSRQVASQLDAVERDTLSVMRQQGANVIELAVVPTFGTQWLLPRLKDFQQRH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
PEVTVNLTNRTRPFLFADTPFDAAIYFGDADWSGTQAHRLMGENPMPVCSPALLGGQGSL
CEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCC
DAGRIAELPLLQQTTRPYAWRQWFDGLGMSVERDMTGPRYELFSMLAQAAMHEMGIALIP
CCCCHHCCCHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHCC
PFLIQRELEEGRLVVANRHALASDKAYHLMIPERKVESASLRAFRDWLVAQAQQYTAKN
HHHHHHHHCCCCEEEEECCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRKIPSTTALVCFEAAARHESFTKAAQELALTQGAVCRQIGGLEAFLNVELFRRSRRGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
KLTEAGLSYSRQVASQLDAVERDTLSVMRQQGANVIELAVVPTFGTQWLLPRLKDFQQRH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
PEVTVNLTNRTRPFLFADTPFDAAIYFGDADWSGTQAHRLMGENPMPVCSPALLGGQGSL
CEEEEEECCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCC
DAGRIAELPLLQQTTRPYAWRQWFDGLGMSVERDMTGPRYELFSMLAQAAMHEMGIALIP
CCCCHHCCCHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHCC
PFLIQRELEEGRLVVANRHALASDKAYHLMIPERKVESASLRAFRDWLVAQAQQYTAKN
HHHHHHHHCCCCEEEEECCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]