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The map label for this gene is kinB [H]

Identifier: 104779398

GI number: 104779398

Start: 82983

End: 84761

Strand: Reverse

Name: kinB [H]

Synonym: PSEEN0086

Alternate gene names: 104779398

Gene position: 84761-82983 (Counterclockwise)

Preceding gene: 104779399

Following gene: 104779390

Centisome position: 1.44

GC content: 67.06

Gene sequence:

>1779_bases
ATGAAATGGCCGATGAAGCTGCGCACGCGCCTGTTCCTCAGCATCTCGGCGCTGATCACGGTGGCGCTGCTCGGGCTGCT
GCTGGGCCTGGTCAGCGTGATGCAGATGGCCACCCAGCAACAGCGCCTGGTCCAGGACACCACCCACTCGCTGGACCTGG
GCCTGAGGCTGCGGCAGAACCTGGGCGAGCAGTTGAACCTGATGCTCGACGAGGACACCAACCCGAACAACCTGGTCACC
CTGCAGGACCAGTTCCAGACGTTGCTCAACCAGGGCCTGGAGCAGGGCGGTGAGCGTACCGGCTTCAGCAAGGCTGCCAG
CAACTACCAGGCATTCGTCCAGGCTTACCGTGAAAGCCCGGCCCCCGCGCGCAGCATGGGCGTCGAGCAACCACTGGGAG
CCGCCTTCAACCAGGTCCGCGCCGACTTGCTCGACTCCCATCGGCAGGCACTGGAACACATCATCCGCAGCGAAGAGAAA
TCCCGCGACCACGCCCTGCTGGTCAGTGGCCTGCTGGGCCTGATGGGCCTGACAGTGCTGGTGCTGGGCTTCATCACCGC
GCACAACATCGCGCGCCGCTTCGGCCAGCCGATCGAGGCACTGGCCAAGGCCGCCGACCAGGTTGGCCAGGGCAATTTCG
ACGTCACCCTGCCGGTGACGCAAGCCACCGAGCTGAACCAGCTGACCCGCCGCTTCGGCCTGATGGCCGACGCCTTGCGC
AAGCACCAGGCCACCAACATCGACGAACTGCTCGCCGGCCAGCAGCGCTTGCAGGCGGTACTCGACAGCATCGACGACGG
CCTGCTGATCATCGACCGCCAGGGCCGCCTGGAACACCTCAACCCCGTGGCCCAGCGCCAGCTCGGCTGGGACCCCGGCC
GCCTGGGCATGAGCCTGGCCGAAGCCCTGCAGCGCCCGGAACTGGAACAGCAGCTGCGCCAGGTGCTGCGCGGCGGCAGC
CTGGACCGGCCACCGGACGACCTGAATCTGGAAGTGGACGAGGAAAGCCGCCTGCTGACCTACAGCCTGACCCCGGTCAG
CCACCCCCAGGGGCCGATCCTCGGTGCCGTGATGGTGCTGCACGATGTCACCGAGCAGAGAGCCTTCGAGCGCGTGCGTA
GCGAGTTCGTCCTGCGTGCATCCCATGAGCTGCGCACACCGGTCACGGGGATGCACATGGCCTTCGGCCTGCTGCGCGAA
CGCATCAGTTTCCCGGCCGGCGCCCGAGAGAACGACCTGCTCGACACCATTGCCGAGGAAATGCAGCGCCTCACCCAACT
GATCAACGACCTGCTGAACTTCTCCCGCTACCAGAGCGGCCTGCAAAAGCTGGAACTGGCCCCCTGCGCCCTCGACGAGC
TGTTCGAGCGGGCCCAGGCACGCTTCGCCGAACAGGCGGCGAACAAGCAGATCGAGCTGCTCCGCGAACTGGAGCCGCCC
CTGCCACGCATCCAGGCCGACGCGGCGCAGCTCGACCGGGTGCTGGACAACCTGCTGCACAACGCCATCCGCCATACCGC
CAGCGGCGGACGCATCCGCCTGCATGCGCGGCGCCATGCCGAGCGGGTGATCATCAGCGTCGAGGATAACGGCGAGGGCA
TCGCCTATGGGCAGCAGGGGCGGATCTTCGAGCCGTTCGTGCAGGTCGGCCGCAAGAAGGGCGGGGCTGGATTGGGGCTG
GCGCTGTGCAAGGAGATCGTGCAGCTGCATGGTGGCCGCATGGGCGTGTTCTCACGGCCGGGGCAGGGGACGCAGTTCTA
CATGGCGCTGCCTGTCTGA

Upstream 100 bases:

>100_bases
TCGGCGCGGTGCTGGCCACCAGTGACACCCTCGACCAGGCCGCCAAGACCCTGGGTATCGACGCCTCCACGCTGTACCGC
AAGCGCAAGCAGTACAACTT

Downstream 100 bases:

>100_bases
ACCCGCGATGCAGGCATCACCCTGTATGGCACCCGCTTCGCGGGTGATCGCGGCTGAAGCCGCTCCTACGGGCGAGCGCC
GCAGATGCCTCTATTCAAGG

Product: alginate biosynthesis sensor protein KinB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MKWPMKLRTRLFLSISALITVALLGLLLGLVSVMQMATQQQRLVQDTTHSLDLGLRLRQNLGEQLNLMLDEDTNPNNLVT
LQDQFQTLLNQGLEQGGERTGFSKAASNYQAFVQAYRESPAPARSMGVEQPLGAAFNQVRADLLDSHRQALEHIIRSEEK
SRDHALLVSGLLGLMGLTVLVLGFITAHNIARRFGQPIEALAKAADQVGQGNFDVTLPVTQATELNQLTRRFGLMADALR
KHQATNIDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWDPGRLGMSLAEALQRPELEQQLRQVLRGGS
LDRPPDDLNLEVDEESRLLTYSLTPVSHPQGPILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRE
RISFPAGARENDLLDTIAEEMQRLTQLINDLLNFSRYQSGLQKLELAPCALDELFERAQARFAEQAANKQIELLRELEPP
LPRIQADAAQLDRVLDNLLHNAIRHTASGGRIRLHARRHAERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGAGLGL
ALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV

Sequences:

>Translated_592_residues
MKWPMKLRTRLFLSISALITVALLGLLLGLVSVMQMATQQQRLVQDTTHSLDLGLRLRQNLGEQLNLMLDEDTNPNNLVT
LQDQFQTLLNQGLEQGGERTGFSKAASNYQAFVQAYRESPAPARSMGVEQPLGAAFNQVRADLLDSHRQALEHIIRSEEK
SRDHALLVSGLLGLMGLTVLVLGFITAHNIARRFGQPIEALAKAADQVGQGNFDVTLPVTQATELNQLTRRFGLMADALR
KHQATNIDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWDPGRLGMSLAEALQRPELEQQLRQVLRGGS
LDRPPDDLNLEVDEESRLLTYSLTPVSHPQGPILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRE
RISFPAGARENDLLDTIAEEMQRLTQLINDLLNFSRYQSGLQKLELAPCALDELFERAQARFAEQAANKQIELLRELEPP
LPRIQADAAQLDRVLDNLLHNAIRHTASGGRIRLHARRHAERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGAGLGL
ALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV
>Mature_592_residues
MKWPMKLRTRLFLSISALITVALLGLLLGLVSVMQMATQQQRLVQDTTHSLDLGLRLRQNLGEQLNLMLDEDTNPNNLVT
LQDQFQTLLNQGLEQGGERTGFSKAASNYQAFVQAYRESPAPARSMGVEQPLGAAFNQVRADLLDSHRQALEHIIRSEEK
SRDHALLVSGLLGLMGLTVLVLGFITAHNIARRFGQPIEALAKAADQVGQGNFDVTLPVTQATELNQLTRRFGLMADALR
KHQATNIDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWDPGRLGMSLAEALQRPELEQQLRQVLRGGS
LDRPPDDLNLEVDEESRLLTYSLTPVSHPQGPILGAVMVLHDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRE
RISFPAGARENDLLDTIAEEMQRLTQLINDLLNFSRYQSGLQKLELAPCALDELFERAQARFAEQAANKQIELLRELEPP
LPRIQADAAQLDRVLDNLLHNAIRHTASGGRIRLHARRHAERVIISVEDNGEGIAYGQQGRIFEPFVQVGRKKGGAGLGL
ALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=516, Percent_Identity=25.3875968992248, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1786600, Length=350, Percent_Identity=31.4285714285714, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=32.421875, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=31.0924369747899, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=31.1688311688312, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790436, Length=212, Percent_Identity=34.4339622641509, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786912, Length=224, Percent_Identity=32.5892857142857, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788393, Length=229, Percent_Identity=29.6943231441048, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=26.7806267806268, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788713, Length=363, Percent_Identity=24.5179063360882, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=29.4372294372294, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=31.0502283105023, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI48994928, Length=245, Percent_Identity=27.3469387755102, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1790861, Length=202, Percent_Identity=32.6732673267327, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=216, Percent_Identity=27.3148148148148, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=221, Percent_Identity=30.316742081448, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790551, Length=221, Percent_Identity=30.316742081448, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65829; Mature: 65829

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWPMKLRTRLFLSISALITVALLGLLLGLVSVMQMATQQQRLVQDTTHSLDLGLRLRQN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEQLNLMLDEDTNPNNLVTLQDQFQTLLNQGLEQGGERTGFSKAASNYQAFVQAYRESP
CHHHHEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
APARSMGVEQPLGAAFNQVRADLLDSHRQALEHIIRSEEKSRDHALLVSGLLGLMGLTVL
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLGFITAHNIARRFGQPIEALAKAADQVGQGNFDVTLPVTQATELNQLTRRFGLMADALR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
KHQATNIDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWDPGRLGMSLA
HHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCHHHHHHCCCCHHHHHHHHH
EALQRPELEQQLRQVLRGGSLDRPPDDLNLEVDEESRLLTYSLTPVSHPQGPILGAVMVL
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
HDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRERISFPAGARENDLLDTIAEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MQRLTQLINDLLNFSRYQSGLQKLELAPCALDELFERAQARFAEQAANKQIELLRELEPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPRIQADAAQLDRVLDNLLHNAIRHTASGGRIRLHARRHAERVIISVEDNGEGIAYGQQG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCEEEEEEECCCCEEEECCCC
RIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKWPMKLRTRLFLSISALITVALLGLLLGLVSVMQMATQQQRLVQDTTHSLDLGLRLRQN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEQLNLMLDEDTNPNNLVTLQDQFQTLLNQGLEQGGERTGFSKAASNYQAFVQAYRESP
CHHHHEEEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
APARSMGVEQPLGAAFNQVRADLLDSHRQALEHIIRSEEKSRDHALLVSGLLGLMGLTVL
CCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VLGFITAHNIARRFGQPIEALAKAADQVGQGNFDVTLPVTQATELNQLTRRFGLMADALR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
KHQATNIDELLAGQQRLQAVLDSIDDGLLIIDRQGRLEHLNPVAQRQLGWDPGRLGMSLA
HHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCHHHHHHCCCCHHHHHHHHH
EALQRPELEQQLRQVLRGGSLDRPPDDLNLEVDEESRLLTYSLTPVSHPQGPILGAVMVL
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHH
HDVTEQRAFERVRSEFVLRASHELRTPVTGMHMAFGLLRERISFPAGARENDLLDTIAEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MQRLTQLINDLLNFSRYQSGLQKLELAPCALDELFERAQARFAEQAANKQIELLRELEPP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPRIQADAAQLDRVLDNLLHNAIRHTASGGRIRLHARRHAERVIISVEDNGEGIAYGQQG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCEEEEEEECCCCEEEECCCC
RIFEPFVQVGRKKGGAGLGLALCKEIVQLHGGRMGVFSRPGQGTQFYMALPV
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]