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The map label for this gene is gidB

Identifier: 94986432

GI number: 94986432

Start: 2455417

End: 2456154

Strand: Reverse

Name: gidB

Synonym: Dgeo_2335

Alternate gene names: 94986432

Gene position: 2456154-2455417 (Counterclockwise)

Preceding gene: 94986433

Following gene: 94986431

Centisome position: 99.55

GC content: 67.07

Gene sequence:

>738_bases
ATGACGCCGGAGGGACGCGCCCTGCTTCTGGCGGGAGCCGCCGAGCTGGGCCTTGAGCTGAGTGCAGAGCACCTCGACCG
CTTCGCGCGGCTGCTGGTGCGGCTGACCGAAGGCTCAGCCCAGCTCAACCTGACGGCCCTGCATCAAGAGCGCGACATCG
TGCTCAAGCATTTCGTAGACTCGCTGACCTGTCTGCGTGGGGGGTGGCTGGACGGGGCGGCGCGGGTGCTGGATCTCGGC
ACCGGGGCAGGATTTCCAGCGCTTCCGCTGGCGATCGTGCGGCCCGACCTTCAACTTGTGGCGCTGGATGCCACCCGCAA
AAAGGTAGATTTTGTGGAGCGGACGGCGCGGAGCCTCGAACTGAACCATGTCCAGCCCCTGACCGGGCGGGCCGAAACCC
TGGGACGCGATCCGGCACAGCGGGAAAGCTATGACCGGGTGGTGACCCGTGCGGTGGCGGCCCTTCCCATCCTGGCGGAA
CTCACGCTGCCGTTCCTGCGAATGGGAGGCTTTCTCCTGGCCCAGAAGGGACCGATTGGCCCCGAAGAACTGGAGGCGGG
TACGCGCGCGGCCCAGGAGGTCGGCGGGGAGATTCGGGCCATTGATGCCTTTGTTTTGCCGGTTGCAGGAGATGCCCGTA
CCCTGGTGGTGGTTGAGAAAACCGCGCCCACCCCCGAGCGTTACCCGCGCCGCGAGGGCGTGCCGAACCGCAAGCCGTTA
TTCTGGCGGGCGACGTGA

Upstream 100 bases:

>100_bases
TGCGCGAGCGGCTGGTGCCGCTGTTGGACGCCCTGCCGCTGGAGGAGATCGATGAACACGTATACGAGCGTTACCAAGCG
ATGAAACTGAGGTACGGCCT

Downstream 100 bases:

>100_bases
AGACGCTCGGAGTCGTGAATCAGAAGGGGGGGGTGGGAAAGACAACCACCGCCATCAACCTGGCCGCCTACCTCGCGGCG
GGTGGGCGCCGGGTGCTGCT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTPEGRALLLAGAAELGLELSAEHLDRFARLLVRLTEGSAQLNLTALHQERDIVLKHFVDSLTCLRGGWLDGAARVLDLG
TGAGFPALPLAIVRPDLQLVALDATRKKVDFVERTARSLELNHVQPLTGRAETLGRDPAQRESYDRVVTRAVAALPILAE
LTLPFLRMGGFLLAQKGPIGPEELEAGTRAAQEVGGEIRAIDAFVLPVAGDARTLVVVEKTAPTPERYPRREGVPNRKPL
FWRAT

Sequences:

>Translated_245_residues
MTPEGRALLLAGAAELGLELSAEHLDRFARLLVRLTEGSAQLNLTALHQERDIVLKHFVDSLTCLRGGWLDGAARVLDLG
TGAGFPALPLAIVRPDLQLVALDATRKKVDFVERTARSLELNHVQPLTGRAETLGRDPAQRESYDRVVTRAVAALPILAE
LTLPFLRMGGFLLAQKGPIGPEELEAGTRAAQEVGGEIRAIDAFVLPVAGDARTLVVVEKTAPTPERYPRREGVPNRKPL
FWRAT
>Mature_244_residues
TPEGRALLLAGAAELGLELSAEHLDRFARLLVRLTEGSAQLNLTALHQERDIVLKHFVDSLTCLRGGWLDGAARVLDLGT
GAGFPALPLAIVRPDLQLVALDATRKKVDFVERTARSLELNHVQPLTGRAETLGRDPAQRESYDRVVTRAVAALPILAEL
TLPFLRMGGFLLAQKGPIGPEELEAGTRAAQEVGGEIRAIDAFVLPVAGDARTLVVVEKTAPTPERYPRREGVPNRKPLF
WRAT

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=213, Percent_Identity=26.2910798122066, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_DEIGD (Q1IVV7)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605796.1
- ProteinModelPortal:   Q1IVV7
- SMR:   Q1IVV7
- STRING:   Q1IVV7
- GeneID:   4057188
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2335
- NMPDR:   fig|68909.1.peg.1442
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- PhylomeDB:   Q1IVV7
- ProtClustDB:   PRK00107
- BioCyc:   DGEO319795:DGEO_2335-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26611; Mature: 26480

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEGRALLLAGAAELGLELSAEHLDRFARLLVRLTEGSAQLNLTALHQERDIVLKHFVD
CCCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
SLTCLRGGWLDGAARVLDLGTGAGFPALPLAIVRPDLQLVALDATRKKVDFVERTARSLE
HHHHHHCCCCCCHHHEEECCCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHC
LNHVQPLTGRAETLGRDPAQRESYDRVVTRAVAALPILAELTLPFLRMGGFLLAQKGPIG
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
PEELEAGTRAAQEVGGEIRAIDAFVLPVAGDARTLVVVEKTAPTPERYPRREGVPNRKPL
HHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCCCE
FWRAT
EEECC
>Mature Secondary Structure 
TPEGRALLLAGAAELGLELSAEHLDRFARLLVRLTEGSAQLNLTALHQERDIVLKHFVD
CCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
SLTCLRGGWLDGAARVLDLGTGAGFPALPLAIVRPDLQLVALDATRKKVDFVERTARSLE
HHHHHHCCCCCCHHHEEECCCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHC
LNHVQPLTGRAETLGRDPAQRESYDRVVTRAVAALPILAELTLPFLRMGGFLLAQKGPIG
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC
PEELEAGTRAAQEVGGEIRAIDAFVLPVAGDARTLVVVEKTAPTPERYPRREGVPNRKPL
HHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCCCCCE
FWRAT
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA