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The map label for this gene is sseA [C]

Identifier: 94986305

GI number: 94986305

Start: 2327542

End: 2328402

Strand: Reverse

Name: sseA [C]

Synonym: Dgeo_2206

Alternate gene names: 94986305

Gene position: 2328402-2327542 (Counterclockwise)

Preceding gene: 94986307

Following gene: 94986303

Centisome position: 94.37

GC content: 63.88

Gene sequence:

>861_bases
ATGGAATACGCGAAAGACGTGCTGGTGAGCACCGACTGGGTGGCGCAGAACCTGAACCGCGAAGGCGTGCGCCTGATTGA
GGTGGACGAGGACATTCTGCTGTACGACACAGGCCACATCCCCGGCGCGGTCAAAATCGACTGGCAGCAGGACTTCTGGG
ACCCGGTGATGCGCGAGTTCATTCAGCCCGAGCAGCTACAGGCCTTGCTGGGCCGGCTGGGCCTGAAGCCCGAAGACACC
ATTGTTCTGTATGGTGACAAGAGCAACTGGTGGGCCGCCTATGCCTACTGGTTCCTGACCTACAACGGCGTGCAGAACCT
CAAGCTGATGAACGGCGGTCGCCAGAAGTGGGTGGCGGAAGGTCGCGAACTCACCACCGAGGTCCCCAGCTACGCGCCCA
CCGAGTACCCGCCCCTCCAGCGCGACGAGAGCCTGCGCGCCTACCGCGACGAGGTGAAGGCGCATATTGAGGCGGTCCGC
GCCGGCCAGGGCGCGATGGTGGATGTGCGCAGCCCCGACGAGTTTTCCGGCAAAGTCACGCACATGCCCAACTATCCGCA
GGAAGGCGTGCTGCGCGGCGGTCATATTCCCGGCGCCCGCAACATTCCCTGGGCACGGGCCACCAACGAGGACGGCACCT
TTAAGAGCGCGGACGAACTGAAGGCGCTCTACGAGGGCGAGGGCGTGACGCCGGACAAGGACGTGATCGCCTACTGCCGG
ATCGCGGAACGCAGCAGCCACTCTTGGTTCGTGTTGCGCGAACTGCTGGGCTACCCCAAGGTTCGCAACTATGACGGCTC
GTGGACCGAGTGGGGCAACGCGGTGGGGATGCCCATCGAGAAGACGTACCAGGAAGCCTGA

Upstream 100 bases:

>100_bases
CCAGGTCCCGAGGCGGTATGGTAAGTCCTGTCCAGTTGACCTTTTTGGTCAATGAAAGACCCAAGTGGGTACAATCGGCT
TCGGAAGCGAGGAGGCAGAG

Downstream 100 bases:

>100_bases
GCCCCCACCACGGCGCAGGGCAAAAGCAGAGCAGTCTCTGGTTGCTCTGCTTTTTGTTGCTTTTTCCGCGGCCGCCCCTC
CAGTCAGGGGAAGCGTCAGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MEYAKDVLVSTDWVAQNLNREGVRLIEVDEDILLYDTGHIPGAVKIDWQQDFWDPVMREFIQPEQLQALLGRLGLKPEDT
IVLYGDKSNWWAAYAYWFLTYNGVQNLKLMNGGRQKWVAEGRELTTEVPSYAPTEYPPLQRDESLRAYRDEVKAHIEAVR
AGQGAMVDVRSPDEFSGKVTHMPNYPQEGVLRGGHIPGARNIPWARATNEDGTFKSADELKALYEGEGVTPDKDVIAYCR
IAERSSHSWFVLRELLGYPKVRNYDGSWTEWGNAVGMPIEKTYQEA

Sequences:

>Translated_286_residues
MEYAKDVLVSTDWVAQNLNREGVRLIEVDEDILLYDTGHIPGAVKIDWQQDFWDPVMREFIQPEQLQALLGRLGLKPEDT
IVLYGDKSNWWAAYAYWFLTYNGVQNLKLMNGGRQKWVAEGRELTTEVPSYAPTEYPPLQRDESLRAYRDEVKAHIEAVR
AGQGAMVDVRSPDEFSGKVTHMPNYPQEGVLRGGHIPGARNIPWARATNEDGTFKSADELKALYEGEGVTPDKDVIAYCR
IAERSSHSWFVLRELLGYPKVRNYDGSWTEWGNAVGMPIEKTYQEA
>Mature_286_residues
MEYAKDVLVSTDWVAQNLNREGVRLIEVDEDILLYDTGHIPGAVKIDWQQDFWDPVMREFIQPEQLQALLGRLGLKPEDT
IVLYGDKSNWWAAYAYWFLTYNGVQNLKLMNGGRQKWVAEGRELTTEVPSYAPTEYPPLQRDESLRAYRDEVKAHIEAVR
AGQGAMVDVRSPDEFSGKVTHMPNYPQEGVLRGGHIPGARNIPWARATNEDGTFKSADELKALYEGEGVTPDKDVIAYCR
IAERSSHSWFVLRELLGYPKVRNYDGSWTEWGNAVGMPIEKTYQEA

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=279, Percent_Identity=29.3906810035842, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI61835204, Length=281, Percent_Identity=29.1814946619217, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI194473681, Length=281, Percent_Identity=29.1814946619217, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI194473668, Length=281, Percent_Identity=29.1814946619217, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI87082121, Length=284, Percent_Identity=28.5211267605634, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87081967, Length=254, Percent_Identity=28.3464566929134, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=247, Percent_Identity=26.7206477732794, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997283, Length=302, Percent_Identity=23.841059602649, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=221, Percent_Identity=28.5067873303167, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32630; Mature: 32630

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYAKDVLVSTDWVAQNLNREGVRLIEVDEDILLYDTGHIPGAVKIDWQQDFWDPVMREF
CCCHHHHHHCCHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEEECCCHHHHHHHHHHH
IQPEQLQALLGRLGLKPEDTIVLYGDKSNWWAAYAYWFLTYNGVQNLKLMNGGRQKWVAE
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCEEEECCCCHHHHHC
GRELTTEVPSYAPTEYPPLQRDESLRAYRDEVKAHIEAVRAGQGAMVDVRSPDEFSGKVT
CCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEE
HMPNYPQEGVLRGGHIPGARNIPWARATNEDGTFKSADELKALYEGEGVTPDKDVIAYCR
ECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
IAERSSHSWFVLRELLGYPKVRNYDGSWTEWGNAVGMPIEKTYQEA
HHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MEYAKDVLVSTDWVAQNLNREGVRLIEVDEDILLYDTGHIPGAVKIDWQQDFWDPVMREF
CCCHHHHHHCCHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEEECCCHHHHHHHHHHH
IQPEQLQALLGRLGLKPEDTIVLYGDKSNWWAAYAYWFLTYNGVQNLKLMNGGRQKWVAE
CCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCCEEEECCCCHHHHHC
GRELTTEVPSYAPTEYPPLQRDESLRAYRDEVKAHIEAVRAGQGAMVDVRSPDEFSGKVT
CCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEE
HMPNYPQEGVLRGGHIPGARNIPWARATNEDGTFKSADELKALYEGEGVTPDKDVIAYCR
ECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHH
IAERSSHSWFVLRELLGYPKVRNYDGSWTEWGNAVGMPIEKTYQEA
HHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]