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The map label for this gene is hemD [H]

Identifier: 94986230

GI number: 94986230

Start: 2242346

End: 2243869

Strand: Reverse

Name: hemD [H]

Synonym: Dgeo_2131

Alternate gene names: 94986230

Gene position: 2243869-2242346 (Counterclockwise)

Preceding gene: 94986231

Following gene: 94986229

Centisome position: 90.95

GC content: 70.41

Gene sequence:

>1524_bases
ATGACTGCTGCCGCAACTTCTTCTTCCTCCCGCGCCTTTGTCTCGCTGATCGGGGCGGGGCCGGGTGATCCGGGCCTGCT
GACCCTGCGGGGAGCCGAGGCGCTTCGGCAGGCGGACGTGGTGTTGTTCGACTACCTGGCGAATCCCGACCTGCTGCGCT
TTTGCCCCCAGGCCGAGACGATCTATGTGGGCAAGAAGGGCTTTTCGGAGTACATCAGCCAGGAACAGATCAACGCGCTG
ATCGTAGAAAAGGCGCGGGAGGGGGGCGGAAAGCGGGTCGCGCGGCTCAAGGGCGGGGACGTGTTCGTGTTTGGGCGCGG
CGGCGAGGAGGCCGAGGCGTGCGTGCAGGCTGGGATTCCCTTCGAGATTGTGCCGGGGGTGACCAGCGCCATCGCTGCCC
CTGCCTACGCCGGAATTCCGGTGACCCACCGCGAAGCCGCACGGAGTTTCGCCGTGCTGACCGGCAACACCCAGGAAGGG
GGTGCCCGTTACGAGACGCTCGCGGGGGTCGATACCCTGGTGCTGCTCATGGGCGTGCGGAATCTCGATCAGATCAGCGC
GGATCTGATCGCCGCGGGCCGCGATCCGCAGACGCCCGCCGCAACGGTGCAGTGGGGCACCACACCGCAGCAGCGCGTGG
CGAGCGGCACCCTGGCTACCATCGCCCAGCGGGTGCGCGAGGCCGGTCTGGAAGCGCCCGCCGTGACGGTGGTGGGCGAG
GTGGCCCGCCTGCGGGAGACGCTGCGCTGGTTTGATGTGGCGCCCGCCGGTCCGCTCGCCGGTCGAACGGTCGCCGTGAC
CCGCACGCGGGAGGGCGCGAGTGCTCTCTCGGAACTGCTGCGTGCCCGCGGGGCGAGCGTGCTCGAAGTGCCGCTGATTC
GCTTCGCCGCGACGGAGAACGCGGCGGCCCTGCAGACTCGCCTGCGTGACCTGAGCGGCGTGACCTGGCTCCTCCTGACC
AGCAACCAGGCAGTCACGGCGCTGTTTGCGCATCTGGAGCACCTGGGTCTGGACGCGCGGCACCTCGCGGGGGTCAAGGT
GGCGGCGGTCGGCCCCAGCACGGCTCGCTCGCTCGCGGAGCAAGGCCTGCGCGCCGACTTTGTGCCCAGCACGCCCGGTG
CCCGGTACTTGGGTGTACAGCTGCCCGCGCGGCCCGGCGAAGTGGCTCTGCACCTCACCAGTCAGGTTGCGGAGGCGGAG
CTGCAACAGACCCTAGAAGCACGCGGCATCCGCTACGAACGAGCCGAACTCTACCGCACTGAACCGGCGCGGCTGAGCAC
GCCGGAGCTGGAACGCCTGCGTGGGGCCGATGTGGTGACTCTCGCTTCGGGCAGCGCGGCGCGGCATCTCGCCGCCCTGG
CAGGCACCGACTTCACGGTGGCGGCGATGGGGCCGCAGACGGCCGACGCGGCACGCGCGGCTGGATTCACGCGGGTGACC
GTTGCGCGGGAGGCGAGTCTGGAAGCTCTGGCCGACGCGGCTGCGCAGGCCATCCGGGAGGAGGCAGAGGCGAGCGGGAG
CTGA

Upstream 100 bases:

>100_bases
GCGCTCACCCTGCCTGAAGACTATGCCTGAGCCGGCGTGTGGTGGACCGCTGCTCAGGCCCCACCCATCTGTCCCGCCCT
CAGCCCGTTAGCCTGGGCGC

Downstream 100 bases:

>100_bases
GGCCCAGTGGAGGGCTGCCGCTGCGGACGCGGGAGGAGCAGCTGCTGGGGGTGCTGACCGTTGGCCGGATTGAACCGGAC
TTCACCCGGCGGGACGAGAC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTAAATSSSSRAFVSLIGAGPGDPGLLTLRGAEALRQADVVLFDYLANPDLLRFCPQAETIYVGKKGFSEYISQEQINAL
IVEKAREGGGKRVARLKGGDVFVFGRGGEEAEACVQAGIPFEIVPGVTSAIAAPAYAGIPVTHREAARSFAVLTGNTQEG
GARYETLAGVDTLVLLMGVRNLDQISADLIAAGRDPQTPAATVQWGTTPQQRVASGTLATIAQRVREAGLEAPAVTVVGE
VARLRETLRWFDVAPAGPLAGRTVAVTRTREGASALSELLRARGASVLEVPLIRFAATENAAALQTRLRDLSGVTWLLLT
SNQAVTALFAHLEHLGLDARHLAGVKVAAVGPSTARSLAEQGLRADFVPSTPGARYLGVQLPARPGEVALHLTSQVAEAE
LQQTLEARGIRYERAELYRTEPARLSTPELERLRGADVVTLASGSAARHLAALAGTDFTVAAMGPQTADAARAAGFTRVT
VAREASLEALADAAAQAIREEAEASGS

Sequences:

>Translated_507_residues
MTAAATSSSSRAFVSLIGAGPGDPGLLTLRGAEALRQADVVLFDYLANPDLLRFCPQAETIYVGKKGFSEYISQEQINAL
IVEKAREGGGKRVARLKGGDVFVFGRGGEEAEACVQAGIPFEIVPGVTSAIAAPAYAGIPVTHREAARSFAVLTGNTQEG
GARYETLAGVDTLVLLMGVRNLDQISADLIAAGRDPQTPAATVQWGTTPQQRVASGTLATIAQRVREAGLEAPAVTVVGE
VARLRETLRWFDVAPAGPLAGRTVAVTRTREGASALSELLRARGASVLEVPLIRFAATENAAALQTRLRDLSGVTWLLLT
SNQAVTALFAHLEHLGLDARHLAGVKVAAVGPSTARSLAEQGLRADFVPSTPGARYLGVQLPARPGEVALHLTSQVAEAE
LQQTLEARGIRYERAELYRTEPARLSTPELERLRGADVVTLASGSAARHLAALAGTDFTVAAMGPQTADAARAAGFTRVT
VAREASLEALADAAAQAIREEAEASGS
>Mature_506_residues
TAAATSSSSRAFVSLIGAGPGDPGLLTLRGAEALRQADVVLFDYLANPDLLRFCPQAETIYVGKKGFSEYISQEQINALI
VEKAREGGGKRVARLKGGDVFVFGRGGEEAEACVQAGIPFEIVPGVTSAIAAPAYAGIPVTHREAARSFAVLTGNTQEGG
ARYETLAGVDTLVLLMGVRNLDQISADLIAAGRDPQTPAATVQWGTTPQQRVASGTLATIAQRVREAGLEAPAVTVVGEV
ARLRETLRWFDVAPAGPLAGRTVAVTRTREGASALSELLRARGASVLEVPLIRFAATENAAALQTRLRDLSGVTWLLLTS
NQAVTALFAHLEHLGLDARHLAGVKVAAVGPSTARSLAEQGLRADFVPSTPGARYLGVQLPARPGEVALHLTSQVAEAEL
QQTLEARGIRYERAELYRTEPARLSTPELERLRGADVVTLASGSAARHLAALAGTDFTVAAMGPQTADAARAAGFTRVTV
AREASLEALADAAAQAIREEAEASGS

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=43.0894308943089, Blast_Score=201, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6322922, Length=232, Percent_Identity=32.7586206896552, Blast_Score=113, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 52946; Mature: 52814

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAATSSSSRAFVSLIGAGPGDPGLLTLRGAEALRQADVVLFDYLANPDLLRFCPQAET
CCCCCCCCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHEEHHHCCCHHHHHCCCCCE
IYVGKKGFSEYISQEQINALIVEKAREGGGKRVARLKGGDVFVFGRGGEEAEACVQAGIP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHCCCC
FEIVPGVTSAIAAPAYAGIPVTHREAARSFAVLTGNTQEGGARYETLAGVDTLVLLMGVR
EEECCCCHHHHHCCCCCCCCCCHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
NLDQISADLIAAGRDPQTPAATVQWGTTPQQRVASGTLATIAQRVREAGLEAPAVTVVGE
CHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VARLRETLRWFDVAPAGPLAGRTVAVTRTREGASALSELLRARGASVLEVPLIRFAATEN
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHEECCCC
AAALQTRLRDLSGVTWLLLTSNQAVTALFAHLEHLGLDARHLAGVKVAAVGPSTARSLAE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCEEEEECCCHHHHHHHH
QGLRADFVPSTPGARYLGVQLPARPGEVALHLTSQVAEAELQQTLEARGIRYERAELYRT
CCCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEHHHHHHCC
EPARLSTPELERLRGADVVTLASGSAARHLAALAGTDFTVAAMGPQTADAARAAGFTRVT
CCCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCEEEE
VAREASLEALADAAAQAIREEAEASGS
EEHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAAATSSSSRAFVSLIGAGPGDPGLLTLRGAEALRQADVVLFDYLANPDLLRFCPQAET
CCCCCCCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHHHEEHHHCCCHHHHHCCCCCE
IYVGKKGFSEYISQEQINALIVEKAREGGGKRVARLKGGDVFVFGRGGEEAEACVQAGIP
EEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHHHHCCCC
FEIVPGVTSAIAAPAYAGIPVTHREAARSFAVLTGNTQEGGARYETLAGVDTLVLLMGVR
EEECCCCHHHHHCCCCCCCCCCHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
NLDQISADLIAAGRDPQTPAATVQWGTTPQQRVASGTLATIAQRVREAGLEAPAVTVVGE
CHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VARLRETLRWFDVAPAGPLAGRTVAVTRTREGASALSELLRARGASVLEVPLIRFAATEN
HHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEHHHHHEECCCC
AAALQTRLRDLSGVTWLLLTSNQAVTALFAHLEHLGLDARHLAGVKVAAVGPSTARSLAE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCEEEEECCCHHHHHHHH
QGLRADFVPSTPGARYLGVQLPARPGEVALHLTSQVAEAELQQTLEARGIRYERAELYRT
CCCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEHHHHHHCC
EPARLSTPELERLRGADVVTLASGSAARHLAALAGTDFTVAAMGPQTADAARAAGFTRVT
CCCCCCCCHHHHHCCCCEEEEECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCEEEE
VAREASLEALADAAAQAIREEAEASGS
EEHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]