Click here to switch to the map view.

The map label for this gene is lacZ [H]

Identifier: 94986203

GI number: 94986203

Start: 2213592

End: 2215475

Strand: Reverse

Name: lacZ [H]

Synonym: Dgeo_2104

Alternate gene names: 94986203

Gene position: 2215475-2213592 (Counterclockwise)

Preceding gene: 94986204

Following gene: 94986195

Centisome position: 89.8

GC content: 70.97

Gene sequence:

>1884_bases
ATGCACGACGAGCACTATCCCCGTCCTCAACTGCGCCGCGCCCATTGGCGGGATCTGGGCGGGCGCTGGGCCTTCGCCTA
CGACGATGGGGCACGCTGGCAGACCCCGGACGAGGTTCACTTCGACCGCGAGATCATCGTGCCGTTTCCCCCGGAGAGCG
CGGCCAGCGGTGTGGGCGACCCCGCCTCTCACCCGGTCGTGTGGTACGGCACCCGGGTGGAGCTGACCGAAGACGAGCGG
CCCCGGCCCGGCGAGCGGCGGCTTCTGTTGCACTTCGGCGCGGTGGACTACCGGGCGCGGGTGTGGGTGAACGGCCAGCT
GGTGGCCGAGCACGAGGGCGGCCACACGCCCTTCAGTGCCGACGTGACCGCATTGGCGGGCGGCCCCGAGCTGCGGATCG
TGGTGCGCGCGGAGGACGACCCGCATGACCTGGCGCAGCCGCGCGGCAAGCAGGACTGGAAGGAGCAGCCGCACTCGATC
TGGTACCGCCGCACCACCGGCATCTGGCAGCCGGTGTGGCTGGAAAGCGTGTCGCAGACCTACCTGCACGAACTGCGCTG
GACGCCCGACCTCGACCGCGAGGAACTGCGCCTCCAGGTGCGCCTGAACGGGGCGCCGCCGCATCCCCTCCAGCTGCGGG
TGCGCCTGAGCCTGCGCGGTCGGCCCCTGGCCCAGCAGACGGTGGCGCTGTCCGGTCGCCAGGGGAGCGCCGTGCTGCCC
CTCAACCACACCCCACTCAACCCCGAGCGCCAGGACCTGCTGTGGTCCCCACGCCACCCCAACCTGATCGACGCGGAGGT
GGCGCTCCTGTCGGAGGACGGGGCGGTGGTGGACGAGGTTCGCAGCTACGCGGGGATGCGCAGCGTGGAGGTCCAGGGGG
GCCGCTTCCTGCTCAACGGCCACCCCTACTACCTGCGGCTGGTGCTGGCGCAGAACTACTGGCCCGAGTCGCACCTCGCC
ACCCCCAGCCCGGAGGCGCTGCGCCGCGAGGCGGAACTGGTCAAGGCGCTGGGCTTCAACGGGGTGCGGATTCACCAGAA
GATCGAGGATCCCCGCTTCCTGTACTGGTGTGACCGCCTGGGCCTGCTGGTGTGGGGCGAGGCGGCCAACGCCTACCGCT
TCACCGACGAGTCGTGCGAGCGCCTGACCCGCGAGTGGCTGGAGGCGCTGCGCCGCGACTACAGCCACCCCTGCATCGTA
GCCTGGGTGCCGCTGAACGAGAGCTGGGGGGTGCCCAACCTGGAACGCGATCCGGCGCAGCGGGCCTTCGTGAAGGGCCT
CTACCACCTCACCAGGGCGCTGGACCCCACCCGCCCGGTCGTGGCGAACGACGGCTGGCAGCACGTGGCAGGCGACATCC
TGGGCATCCACGATTACGCGCTGGAAGGCGCGGTTTTGCGTGAGCGCTACGGGACGCCCGAGGCCATCGAGCGCACCTTT
GCCCACGTGCAGCCGCACTTCCGCAATCTCCTGACCGCCGGGCACACCCGCGGCGAGGAGGCGGTGATGCTCACCGAGTT
CGGCGGCCTGAGCATCCGCCCGGGGGAAGGCGAGCGCTGGTGGGGCTACGGCACGGTGGGCACGCCGGAAGCCTTTTTAG
AGAAGTACGGGGATCTGGTGGGGGCGGTCCTCGACTGCGAGAGCCTGGCGGGCTTCTGCTATACCCAGCTCACCGACACC
GAGCAGGAGACCAACGGCCTGCTGACCGCCAGCCGCCAGCCCAAGTTCGACCTCGCCGCCGCCCGCGCGATCAACACCCG
CCCGTCGCGCGCGGTGCCCGGGGACGTGCTCAACGAGATTCACCAGGCCGCGCAGGAGGAAGACCGGCGCCGCCTTGCCG
GATCCCAGGAAGAACCCCCGGTCCAGCCCCAGCATTCCGGCTGA

Upstream 100 bases:

>100_bases
ACCGCGCTCTCGCCGATGGACCGGCTGGGGAAGAAGTACTCTCGTGTTGTAAAAAAGAGCCGCAAGCCTTTCTGGAAACA
GCAGGGGATCAAGGAGGACC

Downstream 100 bases:

>100_bases
GAACACCACCCCGGGTCGCCGGGGGAGGCGGCTTCTTGGGAGAGAGGGCCGCACAGCACCCGGAATGCCCTCTGCCGAGG
CGGGTTTTCCTTGTGCTGTG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MHDEHYPRPQLRRAHWRDLGGRWAFAYDDGARWQTPDEVHFDREIIVPFPPESAASGVGDPASHPVVWYGTRVELTEDER
PRPGERRLLLHFGAVDYRARVWVNGQLVAEHEGGHTPFSADVTALAGGPELRIVVRAEDDPHDLAQPRGKQDWKEQPHSI
WYRRTTGIWQPVWLESVSQTYLHELRWTPDLDREELRLQVRLNGAPPHPLQLRVRLSLRGRPLAQQTVALSGRQGSAVLP
LNHTPLNPERQDLLWSPRHPNLIDAEVALLSEDGAVVDEVRSYAGMRSVEVQGGRFLLNGHPYYLRLVLAQNYWPESHLA
TPSPEALRREAELVKALGFNGVRIHQKIEDPRFLYWCDRLGLLVWGEAANAYRFTDESCERLTREWLEALRRDYSHPCIV
AWVPLNESWGVPNLERDPAQRAFVKGLYHLTRALDPTRPVVANDGWQHVAGDILGIHDYALEGAVLRERYGTPEAIERTF
AHVQPHFRNLLTAGHTRGEEAVMLTEFGGLSIRPGEGERWWGYGTVGTPEAFLEKYGDLVGAVLDCESLAGFCYTQLTDT
EQETNGLLTASRQPKFDLAAARAINTRPSRAVPGDVLNEIHQAAQEEDRRRLAGSQEEPPVQPQHSG

Sequences:

>Translated_627_residues
MHDEHYPRPQLRRAHWRDLGGRWAFAYDDGARWQTPDEVHFDREIIVPFPPESAASGVGDPASHPVVWYGTRVELTEDER
PRPGERRLLLHFGAVDYRARVWVNGQLVAEHEGGHTPFSADVTALAGGPELRIVVRAEDDPHDLAQPRGKQDWKEQPHSI
WYRRTTGIWQPVWLESVSQTYLHELRWTPDLDREELRLQVRLNGAPPHPLQLRVRLSLRGRPLAQQTVALSGRQGSAVLP
LNHTPLNPERQDLLWSPRHPNLIDAEVALLSEDGAVVDEVRSYAGMRSVEVQGGRFLLNGHPYYLRLVLAQNYWPESHLA
TPSPEALRREAELVKALGFNGVRIHQKIEDPRFLYWCDRLGLLVWGEAANAYRFTDESCERLTREWLEALRRDYSHPCIV
AWVPLNESWGVPNLERDPAQRAFVKGLYHLTRALDPTRPVVANDGWQHVAGDILGIHDYALEGAVLRERYGTPEAIERTF
AHVQPHFRNLLTAGHTRGEEAVMLTEFGGLSIRPGEGERWWGYGTVGTPEAFLEKYGDLVGAVLDCESLAGFCYTQLTDT
EQETNGLLTASRQPKFDLAAARAINTRPSRAVPGDVLNEIHQAAQEEDRRRLAGSQEEPPVQPQHSG
>Mature_627_residues
MHDEHYPRPQLRRAHWRDLGGRWAFAYDDGARWQTPDEVHFDREIIVPFPPESAASGVGDPASHPVVWYGTRVELTEDER
PRPGERRLLLHFGAVDYRARVWVNGQLVAEHEGGHTPFSADVTALAGGPELRIVVRAEDDPHDLAQPRGKQDWKEQPHSI
WYRRTTGIWQPVWLESVSQTYLHELRWTPDLDREELRLQVRLNGAPPHPLQLRVRLSLRGRPLAQQTVALSGRQGSAVLP
LNHTPLNPERQDLLWSPRHPNLIDAEVALLSEDGAVVDEVRSYAGMRSVEVQGGRFLLNGHPYYLRLVLAQNYWPESHLA
TPSPEALRREAELVKALGFNGVRIHQKIEDPRFLYWCDRLGLLVWGEAANAYRFTDESCERLTREWLEALRRDYSHPCIV
AWVPLNESWGVPNLERDPAQRAFVKGLYHLTRALDPTRPVVANDGWQHVAGDILGIHDYALEGAVLRERYGTPEAIERTF
AHVQPHFRNLLTAGHTRGEEAVMLTEFGGLSIRPGEGERWWGYGTVGTPEAFLEKYGDLVGAVLDCESLAGFCYTQLTDT
EQETNGLLTASRQPKFDLAAARAINTRPSRAVPGDVLNEIHQAAQEEDRRRLAGSQEEPPVQPQHSG

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=483, Percent_Identity=25.879917184265, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787903, Length=467, Percent_Identity=26.5524625267666, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1786539, Length=405, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI48994920, Length=382, Percent_Identity=23.0366492146597, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24655438, Length=622, Percent_Identity=24.7588424437299, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI62471735, Length=622, Percent_Identity=24.7588424437299, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922582, Length=622, Percent_Identity=24.7588424437299, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651745, Length=524, Percent_Identity=25.5725190839695, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 71068; Mature: 71068

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDEHYPRPQLRRAHWRDLGGRWAFAYDDGARWQTPDEVHFDREIIVPFPPESAASGVGD
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCC
PASHPVVWYGTRVELTEDERPRPGERRLLLHFGAVDYRARVWVNGQLVAEHEGGHTPFSA
CCCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCC
DVTALAGGPELRIVVRAEDDPHDLAQPRGKQDWKEQPHSIWYRRTTGIWQPVWLESVSQT
CEEEECCCCEEEEEEECCCCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHH
YLHELRWTPDLDREELRLQVRLNGAPPHPLQLRVRLSLRGRPLAQQTVALSGRQGSAVLP
HHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHEECCCCCCEEEE
LNHTPLNPERQDLLWSPRHPNLIDAEVALLSEDGAVVDEVRSYAGMRSVEVQGGRFLLNG
CCCCCCCCCHHHCEECCCCCCEECCEEEEECCCCHHHHHHHHHCCCEEEEECCCEEEECC
HPYYLRLVLAQNYWPESHLATPSPEALRREAELVKALGFNGVRIHQKIEDPRFLYWCDRL
CCEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCC
GLLVWGEAANAYRFTDESCERLTREWLEALRRDYSHPCIVAWVPLNESWGVPNLERDPAQ
CEEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHH
RAFVKGLYHLTRALDPTRPVVANDGWQHVAGDILGIHDYALEGAVLRERYGTPEAIERTF
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCEEHHHHHHCCHHHHHHCCCHHHHHHHH
AHVQPHFRNLLTAGHTRGEEAVMLTEFGGLSIRPGEGERWWGYGTVGTPEAFLEKYGDLV
HHHCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHH
GAVLDCESLAGFCYTQLTDTEQETNGLLTASRQPKFDLAAARAINTRPSRAVPGDVLNEI
HHHHHHHHHHHHHEEECCCCHHHCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
HQAAQEEDRRRLAGSQEEPPVQPQHSG
HHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHDEHYPRPQLRRAHWRDLGGRWAFAYDDGARWQTPDEVHFDREIIVPFPPESAASGVGD
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCC
PASHPVVWYGTRVELTEDERPRPGERRLLLHFGAVDYRARVWVNGQLVAEHEGGHTPFSA
CCCCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCC
DVTALAGGPELRIVVRAEDDPHDLAQPRGKQDWKEQPHSIWYRRTTGIWQPVWLESVSQT
CEEEECCCCEEEEEEECCCCCHHHHCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHH
YLHELRWTPDLDREELRLQVRLNGAPPHPLQLRVRLSLRGRPLAQQTVALSGRQGSAVLP
HHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHHEECCCCCCEEEE
LNHTPLNPERQDLLWSPRHPNLIDAEVALLSEDGAVVDEVRSYAGMRSVEVQGGRFLLNG
CCCCCCCCCHHHCEECCCCCCEECCEEEEECCCCHHHHHHHHHCCCEEEEECCCEEEECC
HPYYLRLVLAQNYWPESHLATPSPEALRREAELVKALGFNGVRIHQKIEDPRFLYWCDRL
CCEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCC
GLLVWGEAANAYRFTDESCERLTREWLEALRRDYSHPCIVAWVPLNESWGVPNLERDPAQ
CEEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHH
RAFVKGLYHLTRALDPTRPVVANDGWQHVAGDILGIHDYALEGAVLRERYGTPEAIERTF
HHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCEEHHHHHHCCHHHHHHCCCHHHHHHHH
AHVQPHFRNLLTAGHTRGEEAVMLTEFGGLSIRPGEGERWWGYGTVGTPEAFLEKYGDLV
HHHCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHH
GAVLDCESLAGFCYTQLTDTEQETNGLLTASRQPKFDLAAARAINTRPSRAVPGDVLNEI
HHHHHHHHHHHHHEEECCCCHHHCCCEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHH
HQAAQEEDRRRLAGSQEEPPVQPQHSG
HHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA