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The map label for this gene is argG

Identifier: 94986166

GI number: 94986166

Start: 2172594

End: 2173838

Strand: Reverse

Name: argG

Synonym: Dgeo_2067

Alternate gene names: 94986166

Gene position: 2173838-2172594 (Counterclockwise)

Preceding gene: 94986167

Following gene: 94986165

Centisome position: 88.11

GC content: 65.46

Gene sequence:

>1245_bases
ATGGCAAACGTTCAGCAGGGTCAGAAAGACAAGATCGTCCTCGCCTACAGTGGCGGCCTCGACACCTCCATTATCCTCAA
ATGGCTCCAGACCGAGCGGAACTACGACGTGGTGACCTTTACCGCCGACCTTGGCCAGGGGGATGAGGTGGAGGAGGCGC
GGGTGAAGGCCCTGAACACCGGTGCGGTCGCCGCCTACGCCCTTGACCTGCGTGAGGAGTTCGTGCGCGACTACGTGTTT
CCGATGTTCCGCGCCGCCGCGCTGTACGAGGGCTACTACCTGCTGGGCACGTCCATCGCCCGGCCCCTCATCGCGAAGAA
GCTGGTCGAGATTGCTGAGAAGGAGGGCGCAGTCGCCGTCTCGCACGGAGCGACCGGCAAGGGCAACGACCAGGTGCGCT
TTGAGATGACCGCCTACGCCCTGAAGCCGGACATCGTGACCGTCGCGCCCTGGCGGGATTGGGACTTCCAGGGCCGCGCG
GACCTCGAGACCTTTGCTCGCGAGCACGGCATTCCTGTCCCGACCACCAAAAAGGATCCTTGGTCCACCGACGCGAACCT
GCTGCACATCTCCTACGAGGGCGGCATTCTGGAAGACCCTTGGGCCGAGCCGCCCGCCCATATGTTCAAGCTGACCGTCT
CTCCCGAGGAGGCACCCGACCAGCCCGAATATGTGGAGGTCGAGTTCGAGAACGGCGACCCGGTGGCCATCGGCGGCGAG
CGCCTCAGCCCCGCCGCCCTGCTGGCGAAGGCAAATGAGATCGGCGGGCGCAACGGCGTGGGCCGCATCGACCTCGTCGA
AAACCGCTTTGTGGGCATGAAGTCGCGCGGTGTCTACGAGACGCCCGGCGGCACGCTGCTCTACCACGCCCGCCGCGCCG
TCGAGAGCCTGACCCTCGACCGCGAGGTGCTGCACCAGCGCGATGCCCTCGCTCCCAAGTACGCTGAACTGGTCTACAAC
GGCTTCTGGTTTGCGCCCGAACGCGAGGCACTTCAGGTCTATATCGACCATGTGGCCCGCAGCGTCACCGGGACTGCTCG
CCTGAAGCTCTACAAGGGCAACTGCACCGTGGTGGGTCGCCGGGCGCCGCGCAGCCTCTACGACAAGGACCTGGTGTCTT
TCGAGGCAGGCGGCGACTACAACCAGCATGACGCCGGGGCCTTTATCAAGCTGAACGCCCTGCGGATGCGGGTGCAGGCG
CGGGTGGACGCCAAGAGGACCCAGCAGGAAGTCGCGCGGGTTTGA

Upstream 100 bases:

>100_bases
GCTGACGTCCCGGCCTTCCCCCAGTGGAGCGGCCGGGTGCGTCACGCCGGGGTGTCTGTGTGTCTTGGCGCGTCCCCCCA
GAAACCGGAGAGAATGCAGC

Downstream 100 bases:

>100_bases
CCGATGGCTCCCACCCTTCGCCCTGCTACCCCTGCCGACGCCCTCGCCTTTCACGCCGTGATGATGGCGGCGGGTATGGA
TCCCCGCAGCAGTTGGAACC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MANVQQGQKDKIVLAYSGGLDTSIILKWLQTERNYDVVTFTADLGQGDEVEEARVKALNTGAVAAYALDLREEFVRDYVF
PMFRAAALYEGYYLLGTSIARPLIAKKLVEIAEKEGAVAVSHGATGKGNDQVRFEMTAYALKPDIVTVAPWRDWDFQGRA
DLETFAREHGIPVPTTKKDPWSTDANLLHISYEGGILEDPWAEPPAHMFKLTVSPEEAPDQPEYVEVEFENGDPVAIGGE
RLSPAALLAKANEIGGRNGVGRIDLVENRFVGMKSRGVYETPGGTLLYHARRAVESLTLDREVLHQRDALAPKYAELVYN
GFWFAPEREALQVYIDHVARSVTGTARLKLYKGNCTVVGRRAPRSLYDKDLVSFEAGGDYNQHDAGAFIKLNALRMRVQA
RVDAKRTQQEVARV

Sequences:

>Translated_414_residues
MANVQQGQKDKIVLAYSGGLDTSIILKWLQTERNYDVVTFTADLGQGDEVEEARVKALNTGAVAAYALDLREEFVRDYVF
PMFRAAALYEGYYLLGTSIARPLIAKKLVEIAEKEGAVAVSHGATGKGNDQVRFEMTAYALKPDIVTVAPWRDWDFQGRA
DLETFAREHGIPVPTTKKDPWSTDANLLHISYEGGILEDPWAEPPAHMFKLTVSPEEAPDQPEYVEVEFENGDPVAIGGE
RLSPAALLAKANEIGGRNGVGRIDLVENRFVGMKSRGVYETPGGTLLYHARRAVESLTLDREVLHQRDALAPKYAELVYN
GFWFAPEREALQVYIDHVARSVTGTARLKLYKGNCTVVGRRAPRSLYDKDLVSFEAGGDYNQHDAGAFIKLNALRMRVQA
RVDAKRTQQEVARV
>Mature_413_residues
ANVQQGQKDKIVLAYSGGLDTSIILKWLQTERNYDVVTFTADLGQGDEVEEARVKALNTGAVAAYALDLREEFVRDYVFP
MFRAAALYEGYYLLGTSIARPLIAKKLVEIAEKEGAVAVSHGATGKGNDQVRFEMTAYALKPDIVTVAPWRDWDFQGRAD
LETFAREHGIPVPTTKKDPWSTDANLLHISYEGGILEDPWAEPPAHMFKLTVSPEEAPDQPEYVEVEFENGDPVAIGGER
LSPAALLAKANEIGGRNGVGRIDLVENRFVGMKSRGVYETPGGTLLYHARRAVESLTLDREVLHQRDALAPKYAELVYNG
FWFAPEREALQVYIDHVARSVTGTARLKLYKGNCTVVGRRAPRSLYDKDLVSFEAGGDYNQHDAGAFIKLNALRMRVQAR
VDAKRTQQEVARV

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=50, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=50, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI1789563, Length=385, Percent_Identity=29.3506493506493, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=45.8033573141487, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI21358151, Length=406, Percent_Identity=47.2906403940887, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_DEIGD (Q1IWM3)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_605530.1
- ProteinModelPortal:   Q1IWM3
- SMR:   Q1IWM3
- STRING:   Q1IWM3
- GeneID:   4058164
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_2067
- NMPDR:   fig|68909.1.peg.2252
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q1IWM3
- ProtClustDB:   PRK00509
- BioCyc:   DGEO319795:DGEO_2067-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45975; Mature: 45844

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 42-42 BINDING 93-93 BINDING 98-98 BINDING 123-123 BINDING 125-125 BINDING 129-129 BINDING 129-129 BINDING 130-130 BINDING 133-133 BINDING 182-182 BINDING 191-191 BINDING 267-267 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVQQGQKDKIVLAYSGGLDTSIILKWLQTERNYDVVTFTADLGQGDEVEEARVKALNT
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCC
GAVAAYALDLREEFVRDYVFPMFRAAALYEGYYLLGTSIARPLIAKKLVEIAEKEGAVAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
SHGATGKGNDQVRFEMTAYALKPDIVTVAPWRDWDFQGRADLETFAREHGIPVPTTKKDP
ECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
WSTDANLLHISYEGGILEDPWAEPPAHMFKLTVSPEEAPDQPEYVEVEFENGDPVAIGGE
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCC
RLSPAALLAKANEIGGRNGVGRIDLVENRFVGMKSRGVYETPGGTLLYHARRAVESLTLD
CCCHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCEECCCCEEEHHHHHHHHHHHHH
REVLHQRDALAPKYAELVYNGFWFAPEREALQVYIDHVARSVTGTARLKLYKGNCTVVGR
HHHHHHHHCCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEC
RAPRSLYDKDLVSFEAGGDYNQHDAGAFIKLNALRMRVQARVDAKRTQQEVARV
CCCHHHHHHHHEEEECCCCCCCCCCCEEEEEHHHHEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANVQQGQKDKIVLAYSGGLDTSIILKWLQTERNYDVVTFTADLGQGDEVEEARVKALNT
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHCCC
GAVAAYALDLREEFVRDYVFPMFRAAALYEGYYLLGTSIARPLIAKKLVEIAEKEGAVAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEE
SHGATGKGNDQVRFEMTAYALKPDIVTVAPWRDWDFQGRADLETFAREHGIPVPTTKKDP
ECCCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
WSTDANLLHISYEGGILEDPWAEPPAHMFKLTVSPEEAPDQPEYVEVEFENGDPVAIGGE
CCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCC
RLSPAALLAKANEIGGRNGVGRIDLVENRFVGMKSRGVYETPGGTLLYHARRAVESLTLD
CCCHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCCCEECCCCEEEHHHHHHHHHHHHH
REVLHQRDALAPKYAELVYNGFWFAPEREALQVYIDHVARSVTGTARLKLYKGNCTVVGR
HHHHHHHHCCCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEC
RAPRSLYDKDLVSFEAGGDYNQHDAGAFIKLNALRMRVQARVDAKRTQQEVARV
CCCHHHHHHHHEEEECCCCCCCCCCCEEEEEHHHHEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA