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The map label for this gene is tuf1

Identifier: 94985969

GI number: 94985969

Start: 1965802

End: 1967019

Strand: Reverse

Name: tuf1

Synonym: Dgeo_1869

Alternate gene names: 94985969

Gene position: 1967019-1965802 (Counterclockwise)

Preceding gene: 94985970

Following gene: 94985968

Centisome position: 79.73

GC content: 64.45

Gene sequence:

>1218_bases
ATGGCAAAAGGAACGTTTGAGCGGACGAAGCCTCACGTGAACGTGGGGACGATCGGACACGTGGACCACGGGAAGACCAC
GCTGACAGCAGCGATTACCTTCACGGCGGCGGCGATGGACCCGACCGTCGAGAAGCTGGCCTACGACCAGATCGACAAGG
CGCCCGAAGAAAAGGCCCGCGGCATCACCATCAACACCGCCCACGTCGAGTACAACACCCCCGCGCGGCACTACAGCCAC
GTCGACTGCCCCGGTCACGCCGACTACGTCAAGAACATGATCACCGGCGCGGCCCAGATGGACGGCGCGATCCTGGTGGT
CTCCTCGGCCGACGGCCCCATGCCCCAGACCCGCGAGCACATCCTGCTGGCCCGTCAGGTGGGCGTGCCCTACATCGTCG
TCTTCATGAACAAGGTCGACATGGTCGACGACGAAGAACTGCTGGAACTCGTCGAGATGGAAGTGCGCGAGCTGCTCTCG
AAGTACGAGTTCCCCGGCGATGATCTGCCGGTGATCAAGGGCAGTGCTCTGCAGGCGCTGGAAGCGCTGCAGCAAAACCC
CAAGACCGCGCGCGGCGAAAACCCGTGGGTCGACAAGATCTGGGAACTGCTGGACGCGATCGACGCCTACATCCCCACCC
CCGAACGCGCCACCGACAAGACCTTCCTGATGCCGGTGGAAGACGTGTTCACCATCACGGGTCGCGGCACCGTGGCCACG
GGCCGCGTGGAACGCGGCGTGTGCAAGGTGGGCGACGAAGTGGAGATCGTGGGGCTGCGCGACACCAAGAAGACCACCAT
CACTGGGGTGGAAATGCACCGCAAGCTGCTGGATCAAGGCATGGCCGGGGACAACGTGGGCGTGCTGCTGCGTGGTGTGG
CGCGTGACGACGTGGAACGCGGGCAGGTGCTGGCCAAGCCGGGCAGCATCACACCGCACACCAAGTTCGAGGCCAGCGTG
TACGTGCTGTCCAAGGATGAAGGGGGCCGTCACTCGGCGTTCTTCGGCGGGTACCGGCCGCAGTTCTACTTCCGCACGAC
GGACGTGACGGGCGTGGTAGAACTGCCCGCGGGCGTGGAAATGGTGATGCCCGGGGATAACGTCAGCTTCACCGTTGAGC
TGATCAAGCCCATCGCCATGGAAGAAGGCCTGCGCTTCGCCATCCGCGAAGGTGGCCGCACCGTCGGCGCTGGCGTCGTC
ACCAAGGTCCTGGAGTAA

Upstream 100 bases:

>100_bases
GGGACACCGCCCTGGCGGGAATCAACCCAATGTGGGAGCGCCCACAGCCCGATCAAAGGGTTTTTTTGGCGTGTCTTCCA
CCGCTTGGAGGAGAGTCATC

Downstream 100 bases:

>100_bases
ACACAATGGTTGCCCCGAAGATTCGTATCAAACTGCGTGGCTTTGACCACAAGGCGCTGGACCAGTCCGCGAGCAAGATC
GTGGACACGGTCCGGCGTAC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAKGTFERTKPHVNVGTIGHVDHGKTTLTAAITFTAAAMDPTVEKLAYDQIDKAPEEKARGITINTAHVEYNTPARHYSH
VDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIVVFMNKVDMVDDEELLELVEMEVRELLS
KYEFPGDDLPVIKGSALQALEALQQNPKTARGENPWVDKIWELLDAIDAYIPTPERATDKTFLMPVEDVFTITGRGTVAT
GRVERGVCKVGDEVEIVGLRDTKKTTITGVEMHRKLLDQGMAGDNVGVLLRGVARDDVERGQVLAKPGSITPHTKFEASV
YVLSKDEGGRHSAFFGGYRPQFYFRTTDVTGVVELPAGVEMVMPGDNVSFTVELIKPIAMEEGLRFAIREGGRTVGAGVV
TKVLE

Sequences:

>Translated_405_residues
MAKGTFERTKPHVNVGTIGHVDHGKTTLTAAITFTAAAMDPTVEKLAYDQIDKAPEEKARGITINTAHVEYNTPARHYSH
VDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIVVFMNKVDMVDDEELLELVEMEVRELLS
KYEFPGDDLPVIKGSALQALEALQQNPKTARGENPWVDKIWELLDAIDAYIPTPERATDKTFLMPVEDVFTITGRGTVAT
GRVERGVCKVGDEVEIVGLRDTKKTTITGVEMHRKLLDQGMAGDNVGVLLRGVARDDVERGQVLAKPGSITPHTKFEASV
YVLSKDEGGRHSAFFGGYRPQFYFRTTDVTGVVELPAGVEMVMPGDNVSFTVELIKPIAMEEGLRFAIREGGRTVGAGVV
TKVLE
>Mature_404_residues
AKGTFERTKPHVNVGTIGHVDHGKTTLTAAITFTAAAMDPTVEKLAYDQIDKAPEEKARGITINTAHVEYNTPARHYSHV
DCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIVVFMNKVDMVDDEELLELVEMEVRELLSK
YEFPGDDLPVIKGSALQALEALQQNPKTARGENPWVDKIWELLDAIDAYIPTPERATDKTFLMPVEDVFTITGRGTVATG
RVERGVCKVGDEVEIVGLRDTKKTTITGVEMHRKLLDQGMAGDNVGVLLRGVARDDVERGQVLAKPGSITPHTKFEASVY
VLSKDEGGRHSAFFGGYRPQFYFRTTDVTGVVELPAGVEMVMPGDNVSFTVELIKPIAMEEGLRFAIREGGRTVGAGVVT
KVLE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=403, Percent_Identity=55.0868486352357, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=30.4635761589404, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=30.9050772626932, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=28.4090909090909, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=28.4090909090909, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI54607086, Length=339, Percent_Identity=28.6135693215339, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018522, Length=365, Percent_Identity=26.5753424657534, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=365, Percent_Identity=26.5753424657534, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=365, Percent_Identity=26.5753424657534, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=28.3536585365854, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI157426893, Length=294, Percent_Identity=26.8707482993197, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790412, Length=404, Percent_Identity=74.7524752475248, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=404, Percent_Identity=74.7524752475248, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=303, Percent_Identity=30.6930693069307, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI48994988, Length=282, Percent_Identity=29.4326241134752, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789108, Length=427, Percent_Identity=26.2295081967213, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=30.6122448979592, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=410, Percent_Identity=50.9756097560976, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25141371, Length=394, Percent_Identity=45.6852791878173, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=455, Percent_Identity=31.4285714285714, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=455, Percent_Identity=31.4285714285714, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=30.1123595505618, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=25.7403189066059, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=25.9090909090909, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=437, Percent_Identity=25.858123569794, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=310, Percent_Identity=28.0645161290323, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=151, Percent_Identity=36.4238410596026, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=450, Percent_Identity=24.6666666666667, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=243, Percent_Identity=27.5720164609054, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=407, Percent_Identity=64.8648648648649, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=451, Percent_Identity=32.5942350332594, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6319594, Length=451, Percent_Identity=32.5942350332594, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6320377, Length=449, Percent_Identity=26.0579064587973, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=464, Percent_Identity=23.2758620689655, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=276, Percent_Identity=28.9855072463768, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=285, Percent_Identity=25.6140350877193, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=170, Percent_Identity=27.6470588235294, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=402, Percent_Identity=56.9651741293532, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=402, Percent_Identity=56.9651741293532, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=397, Percent_Identity=49.6221662468514, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45553807, Length=453, Percent_Identity=30.242825607064, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI45553816, Length=453, Percent_Identity=30.242825607064, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24651721, Length=453, Percent_Identity=30.242825607064, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17864154, Length=453, Percent_Identity=30.242825607064, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=30.9050772626932, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=30.9050772626932, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45550900, Length=440, Percent_Identity=29.3181818181818, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137380, Length=343, Percent_Identity=30.9037900874636, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=27.7955271565495, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_DEIGD (Q1IX70)

Other databases:

- EMBL:   CP000359
- EMBL:   CP000359
- RefSeq:   YP_604117.1
- RefSeq:   YP_605333.1
- ProteinModelPortal:   Q1IX70
- SMR:   Q1IX70
- STRING:   Q1IX70
- GeneID:   4057611
- GeneID:   4058995
- GenomeReviews:   CP000359_GR
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0646
- KEGG:   dge:Dgeo_1869
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q1IX70
- BioCyc:   DGEO319795:DGEO_0646-MONOMER
- BioCyc:   DGEO319795:DGEO_1869-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44215; Mature: 44084

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGTFERTKPHVNVGTIGHVDHGKTTLTAAITFTAAAMDPTVEKLAYDQIDKAPEEKAR
CCCCCCCCCCCCEEECEEECCCCCCEEEEEEEEEEEHHCCHHHHHHHHHHHHCCHHHHHC
GITINTAHVEYNTPARHYSHVDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREH
CEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPYIVVFMNKVDMVDDEELLELVEMEVRELLSKYEFPGDDLPVIKGSALQAL
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHH
EALQQNPKTARGENPWVDKIWELLDAIDAYIPTPERATDKTFLMPVEDVFTITGRGTVAT
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHEEEEECCCEEEE
GRVERGVCKVGDEVEIVGLRDTKKTTITGVEMHRKLLDQGMAGDNVGVLLRGVARDDVER
CCHHCCHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEEEHHHCCHHC
GQVLAKPGSITPHTKFEASVYVLSKDEGGRHSAFFGGYRPQFYFRTTDVTGVVELPAGVE
CEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEECCCCCEEEEEECCCCEEEECCCCCE
MVMPGDNVSFTVELIKPIAMEEGLRFAIREGGRTVGAGVVTKVLE
EEECCCCCEEEEHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKGTFERTKPHVNVGTIGHVDHGKTTLTAAITFTAAAMDPTVEKLAYDQIDKAPEEKAR
CCCCCCCCCCCEEECEEECCCCCCEEEEEEEEEEEHHCCHHHHHHHHHHHHCCHHHHHC
GITINTAHVEYNTPARHYSHVDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREH
CEEEEEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
ILLARQVGVPYIVVFMNKVDMVDDEELLELVEMEVRELLSKYEFPGDDLPVIKGSALQAL
HHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHH
EALQQNPKTARGENPWVDKIWELLDAIDAYIPTPERATDKTFLMPVEDVFTITGRGTVAT
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHEEEEECCCEEEE
GRVERGVCKVGDEVEIVGLRDTKKTTITGVEMHRKLLDQGMAGDNVGVLLRGVARDDVER
CCHHCCHHCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCCCCHHHEEEEHHHCCHHC
GQVLAKPGSITPHTKFEASVYVLSKDEGGRHSAFFGGYRPQFYFRTTDVTGVVELPAGVE
CEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEECCCCCEEEEEECCCCEEEECCCCCE
MVMPGDNVSFTVELIKPIAMEEGLRFAIREGGRTVGAGVVTKVLE
EEECCCCCEEEEHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA