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The map label for this gene is glnA [H]

Identifier: 94985305

GI number: 94985305

Start: 1278845

End: 1281001

Strand: Reverse

Name: glnA [H]

Synonym: Dgeo_1203

Alternate gene names: 94985305

Gene position: 1281001-1278845 (Counterclockwise)

Preceding gene: 94985313

Following gene: 94985304

Centisome position: 51.92

GC content: 63.61

Gene sequence:

>2157_bases
ATGAACCACGACTTCGACGTGCTTTCTGCCGCGCGCAACTGGCGCGTGGAAACCCCAACCCGCGCGACGCCTGCTCAAAT
TGTCAGCGAGCTCTTTGCCAGCGACGTGCTCACCCTTGAAGAACTCAAGACCCGGCTGTCGAAGCCCGCCTACAAGAGCC
TGCGCGGTACGGTAGAACGCGGTGAGACGCTCGATCCGGCCATTGCGGATACCGTGGCGCTGGCCATGAAGACCTGGGCG
ATGGAAAAGGGTGCCACCCACTACACCCACTGGTTCCAGCCCCTGACTGGCGCGACCGCCGAGAAGCACGACGCCTTTCT
GTCGCCTACGGGGGACGGCGGGGCCATCGCGGCCTTCAGTGGCAAGGAGTTGATTCAGGCCGAGCCTGACGCCTCATCCT
TTCCTTCGGGAGGGCTGCGCGCGACCTTTGAGGCCCGCGGCTACACAGCCTGGGATCCCTCCAGCCCGGCCTTCATCATG
CGGCATGCCAACGGCGCGACCCTCTGCATCCCGACGGCCTTCGCCTCCTGGACCGGAGAGGCACTGGATCTCAAGACGCC
GCTGCTGCGTTCGGTCGAGGCACTGAACCGGGCCGTGACGCCGGCGCTGCACCTCTTTGGGGCCTCGCTGGGCACCCGGG
TCGGCAGCACGCTCGGTGCCGAGCAGGAATACTTCTTGATTGCGGAAGAATACTTCTATCGCCGGCCCGATCTGGTGATG
ACGGGCCGGACCCTCTTCGGCGCGCAGCCGCCACGTGGCCAGGAGCTGGAGGATCACTACTTCGGCGCAATTCCCGACCG
GGTGCTGAGCTTTATGACCGATGCCGAGCTGCAACTCTACGCCCTGGGCATCCCCGTCAAGACCCGCCACAACGAGGTCG
CGCCTGGCCAATTCGAGATCGCGCCCATCTTTGAGAGCAGCAACGTGGCCGCCGACCACCAGCAGCTCATCATGCAGGTG
CTGCGCAACACCGCTCGCAAGTACGGCCTGGTTGCGCTGCTGCACGAAAAACCTTTTGCTGGCGTGAACGGCTCGGGCAA
GCACTGTAACTGGAGCATGAGTACCGACGCCGGCGAGAACCTGCTCGAACCCGGCGACACGCCGCACGAGAACCTGCAGT
TCCTGTTTTTCTGCGCGGCGGTTCTCAAGGCCGTAGACGAGCATCAGGATCTGCTGCGGATCTCGGTTGCCAGCGCCAGC
AATGACCACCGCCTGGGTGCAAACGAGGCCCCGCCTGCCATCATCTCGATCTTCTTGGGCAGCGAGTTGACCGACATCTT
CGACCGCCTGGAAAGCGGCGAGGGCGGACGCGGAACAGAAGCGGGTCTGTTGGGCCTCGGTTCCCCCGTGCTGCCGCCAC
TGCCGCGTCACGCCGGAGACCGCAACCGCACCAGTCCCTTTGCCTTTACCGGCAACAAGTTTGAGTTCCGGGCGGTTGGC
AGCAGCCAAAGCATCAGCTTTCCGATTACCGTCCTGAACACCATCGTCGCGGACGCTGTGCAAAGCCTCACCGAGCAACT
CAAGGCCAAGCTGCAAAACGGGCTAGACCTCGCTGAGGCAGTGGGTGAGATTGTGCGCGACACCTACCGCCAGCACAAGC
GCATCGTATTTAACGGGGATGGGTACAGCCAGGAATGGCACCAGGAGGCGGAACGGCGTGGTCTGCTGAACCTCCGCACC
AGCCTGGACGCCATCCCGCACCTGACTGACCCCAAGAACGTGGCGCTGTTTGGGAAGTTCGGCGTGCTGTCCGAGCGGGA
ACTGGCAGCCCGGCAGGAAATCATGTACGACATCTACTTCAAGACGGTGAACATCGAGGGTGAGACGACCGAGTACATCG
CCCAAACGATGATCCTCCCCGCCGCCGCCGAGTATCTGGGCGATCTGAGTGCCGTGCAGAGGCCCAGCCGTGCCCTCGAC
GCGACCGCGACCGAGGTGACTCAACTCACCGACGCGTTGTATGACGCCCTCCAGAACTTGCGCGAGCAAAACCGGGCGTT
GGGTGGCGATGAGATCGTCGAGAAAGCGCACCACGTCCGCGACCACGTCTTGCCGGCGATGCAGGAGGTTCGCCGGGCCG
CCGACAGTCTGGAAAAGGTTGTGGCCGAGAAGCACTGGCCGCTGCCGACCTACCGGCAGATGATGTTCGTGAAGTGA

Upstream 100 bases:

>100_bases
CAGCTTGTCTCGTCTTCGTTGACCATCTTGTTGTGGGCTGCCTATACTCGGAGCAGTTGGAGGACGCGTCACGCTGCCTG
CCCACCCGGAGGAACCCCGC

Downstream 100 bases:

>100_bases
TGTTCGTGAAGTCGGCGGGGAGTGCCCAGCCCCTGGCCGCAGCACCAGAGAGGGAGCCGGCTCGGAGTGGCTCCCTCTCT
GTGTTGCGGTTGCTGATTGC

Product: glutamine synthetase, catalytic region

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MNHDFDVLSAARNWRVETPTRATPAQIVSELFASDVLTLEELKTRLSKPAYKSLRGTVERGETLDPAIADTVALAMKTWA
MEKGATHYTHWFQPLTGATAEKHDAFLSPTGDGGAIAAFSGKELIQAEPDASSFPSGGLRATFEARGYTAWDPSSPAFIM
RHANGATLCIPTAFASWTGEALDLKTPLLRSVEALNRAVTPALHLFGASLGTRVGSTLGAEQEYFLIAEEYFYRRPDLVM
TGRTLFGAQPPRGQELEDHYFGAIPDRVLSFMTDAELQLYALGIPVKTRHNEVAPGQFEIAPIFESSNVAADHQQLIMQV
LRNTARKYGLVALLHEKPFAGVNGSGKHCNWSMSTDAGENLLEPGDTPHENLQFLFFCAAVLKAVDEHQDLLRISVASAS
NDHRLGANEAPPAIISIFLGSELTDIFDRLESGEGGRGTEAGLLGLGSPVLPPLPRHAGDRNRTSPFAFTGNKFEFRAVG
SSQSISFPITVLNTIVADAVQSLTEQLKAKLQNGLDLAEAVGEIVRDTYRQHKRIVFNGDGYSQEWHQEAERRGLLNLRT
SLDAIPHLTDPKNVALFGKFGVLSERELAARQEIMYDIYFKTVNIEGETTEYIAQTMILPAAAEYLGDLSAVQRPSRALD
ATATEVTQLTDALYDALQNLREQNRALGGDEIVEKAHHVRDHVLPAMQEVRRAADSLEKVVAEKHWPLPTYRQMMFVK

Sequences:

>Translated_718_residues
MNHDFDVLSAARNWRVETPTRATPAQIVSELFASDVLTLEELKTRLSKPAYKSLRGTVERGETLDPAIADTVALAMKTWA
MEKGATHYTHWFQPLTGATAEKHDAFLSPTGDGGAIAAFSGKELIQAEPDASSFPSGGLRATFEARGYTAWDPSSPAFIM
RHANGATLCIPTAFASWTGEALDLKTPLLRSVEALNRAVTPALHLFGASLGTRVGSTLGAEQEYFLIAEEYFYRRPDLVM
TGRTLFGAQPPRGQELEDHYFGAIPDRVLSFMTDAELQLYALGIPVKTRHNEVAPGQFEIAPIFESSNVAADHQQLIMQV
LRNTARKYGLVALLHEKPFAGVNGSGKHCNWSMSTDAGENLLEPGDTPHENLQFLFFCAAVLKAVDEHQDLLRISVASAS
NDHRLGANEAPPAIISIFLGSELTDIFDRLESGEGGRGTEAGLLGLGSPVLPPLPRHAGDRNRTSPFAFTGNKFEFRAVG
SSQSISFPITVLNTIVADAVQSLTEQLKAKLQNGLDLAEAVGEIVRDTYRQHKRIVFNGDGYSQEWHQEAERRGLLNLRT
SLDAIPHLTDPKNVALFGKFGVLSERELAARQEIMYDIYFKTVNIEGETTEYIAQTMILPAAAEYLGDLSAVQRPSRALD
ATATEVTQLTDALYDALQNLREQNRALGGDEIVEKAHHVRDHVLPAMQEVRRAADSLEKVVAEKHWPLPTYRQMMFVK
>Mature_718_residues
MNHDFDVLSAARNWRVETPTRATPAQIVSELFASDVLTLEELKTRLSKPAYKSLRGTVERGETLDPAIADTVALAMKTWA
MEKGATHYTHWFQPLTGATAEKHDAFLSPTGDGGAIAAFSGKELIQAEPDASSFPSGGLRATFEARGYTAWDPSSPAFIM
RHANGATLCIPTAFASWTGEALDLKTPLLRSVEALNRAVTPALHLFGASLGTRVGSTLGAEQEYFLIAEEYFYRRPDLVM
TGRTLFGAQPPRGQELEDHYFGAIPDRVLSFMTDAELQLYALGIPVKTRHNEVAPGQFEIAPIFESSNVAADHQQLIMQV
LRNTARKYGLVALLHEKPFAGVNGSGKHCNWSMSTDAGENLLEPGDTPHENLQFLFFCAAVLKAVDEHQDLLRISVASAS
NDHRLGANEAPPAIISIFLGSELTDIFDRLESGEGGRGTEAGLLGLGSPVLPPLPRHAGDRNRTSPFAFTGNKFEFRAVG
SSQSISFPITVLNTIVADAVQSLTEQLKAKLQNGLDLAEAVGEIVRDTYRQHKRIVFNGDGYSQEWHQEAERRGLLNLRT
SLDAIPHLTDPKNVALFGKFGVLSERELAARQEIMYDIYFKTVNIEGETTEYIAQTMILPAAAEYLGDLSAVQRPSRALD
ATATEVTQLTDALYDALQNLREQNRALGGDEIVEKAHHVRDHVLPAMQEVRRAADSLEKVVAEKHWPLPTYRQMMFVK

Specific function: Unknown

COG id: COG3968

COG function: function code R; Uncharacterized protein related to glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=126, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR022147 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF12437 GSIII_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 78859; Mature: 78859

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHDFDVLSAARNWRVETPTRATPAQIVSELFASDVLTLEELKTRLSKPAYKSLRGTVER
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GETLDPAIADTVALAMKTWAMEKGATHYTHWFQPLTGATAEKHDAFLSPTGDGGAIAAFS
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCCCEECCCCCCCEEEEEC
GKELIQAEPDASSFPSGGLRATFEARGYTAWDPSSPAFIMRHANGATLCIPTAFASWTGE
CCHHEECCCCCCCCCCCCCEEEEECCCEECCCCCCCEEEEEECCCCEEEEECHHHHCCCC
ALDLKTPLLRSVEALNRAVTPALHLFGASLGTRVGSTLGAEQEYFLIAEEYFYRRPDLVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCEEE
TGRTLFGAQPPRGQELEDHYFGAIPDRVLSFMTDAELQLYALGIPVKTRHNEVAPGQFEI
ECCHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCEEE
APIFESSNVAADHQQLIMQVLRNTARKYGLVALLHEKPFAGVNGSGKHCNWSMSTDAGEN
EEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCEEECCCCCCCC
LLEPGDTPHENLQFLFFCAAVLKAVDEHQDLLRISVASASNDHRLGANEAPPAIISIFLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHC
SELTDIFDRLESGEGGRGTEAGLLGLGSPVLPPLPRHAGDRNRTSPFAFTGNKFEFRAVG
CHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECC
SSQSISFPITVLNTIVADAVQSLTEQLKAKLQNGLDLAEAVGEIVRDTYRQHKRIVFNGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCC
GYSQEWHQEAERRGLLNLRTSLDAIPHLTDPKNVALFGKFGVLSERELAARQEIMYDIYF
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEE
KTVNIEGETTEYIAQTMILPAAAEYLGDLSAVQRPSRALDATATEVTQLTDALYDALQNL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
REQNRALGGDEIVEKAHHVRDHVLPAMQEVRRAADSLEKVVAEKHWPLPTYRQMMFVK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
>Mature Secondary Structure
MNHDFDVLSAARNWRVETPTRATPAQIVSELFASDVLTLEELKTRLSKPAYKSLRGTVER
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GETLDPAIADTVALAMKTWAMEKGATHYTHWFQPLTGATAEKHDAFLSPTGDGGAIAAFS
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHCCCEECCCCCCCEEEEEC
GKELIQAEPDASSFPSGGLRATFEARGYTAWDPSSPAFIMRHANGATLCIPTAFASWTGE
CCHHEECCCCCCCCCCCCCEEEEECCCEECCCCCCCEEEEEECCCCEEEEECHHHHCCCC
ALDLKTPLLRSVEALNRAVTPALHLFGASLGTRVGSTLGAEQEYFLIAEEYFYRRPDLVM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCEEE
TGRTLFGAQPPRGQELEDHYFGAIPDRVLSFMTDAELQLYALGIPVKTRHNEVAPGQFEI
ECCHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCEEEEEECCCEECCCCCCCCCCEEE
APIFESSNVAADHQQLIMQVLRNTARKYGLVALLHEKPFAGVNGSGKHCNWSMSTDAGEN
EEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCEEECCCCCCCC
LLEPGDTPHENLQFLFFCAAVLKAVDEHQDLLRISVASASNDHRLGANEAPPAIISIFLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHC
SELTDIFDRLESGEGGRGTEAGLLGLGSPVLPPLPRHAGDRNRTSPFAFTGNKFEFRAVG
CHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECC
SSQSISFPITVLNTIVADAVQSLTEQLKAKLQNGLDLAEAVGEIVRDTYRQHKRIVFNGD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCC
GYSQEWHQEAERRGLLNLRTSLDAIPHLTDPKNVALFGKFGVLSERELAARQEIMYDIYF
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEE
KTVNIEGETTEYIAQTMILPAAAEYLGDLSAVQRPSRALDATATEVTQLTDALYDALQNL
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
REQNRALGGDEIVEKAHHVRDHVLPAMQEVRRAADSLEKVVAEKHWPLPTYRQMMFVK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2576872 [H]