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The map label for this gene is ksgA

Identifier: 94985245

GI number: 94985245

Start: 1215039

End: 1215884

Strand: Reverse

Name: ksgA

Synonym: Dgeo_1143

Alternate gene names: 94985245

Gene position: 1215884-1215039 (Counterclockwise)

Preceding gene: 94985249

Following gene: 94985244

Centisome position: 49.28

GC content: 68.68

Gene sequence:

>846_bases
GTGACCCAGTCTGAACCGCCTTCCCTCCCGCTGTACTCGCCCGCCCGCGTGCGCGACCTGCTCACCCGCCACGGCTTGAG
ACCCACCAAGAGCCTAGGACAGAACTTTCTGATCGACGGCAACATCCTGCGGGCCATCGCGCAGGCAGGTGGGGCCGCCC
CTGGCGTTCCGGTGCTGGAAGTCGGCCCCGGCCTGGGCGTTCTCACGCGCGAACTGGCCGCCCGCGGCGCGCACGTGACT
GCTCTTGAAAAAGATGAGCGCTTGCGTCCCGTCCTGGCGGAGACCCTCGCCGGACAGGACGTTCAGGTGGTCTGGGGGGA
TGCGCTGGAGTTCGATTACGCCAGTCTTCCGGCAGGCACCCGAGTCATCGCCAACCTGCCCTACTACATCACTGGGCCGC
TGCTTGCCCGCTTTATGCAGGCGCCCGGTATCATCTCCGCGACCGTGCTGGTGCAAAAGGAGGTGGCGGGGCGCCTGGCT
GCCCGCCCCGGCGAGGACAACTACGGGTTCCTGAGCGCCCTGGCCGCCCTCTACGGCACGGTTCAGCACGTGCGTGACGT
GCCGAAGGGCGCCTTTCTGCCCGCCCCCGACGTGACCAGCAGCGTCGTCCGGCTGGACTTTGACCGTGCGCGTCCTGCCC
CCGAACCCGCTTTTCTCAAGTTCGTAGAGGCGGCCCTGCACCACCGCCGCAAGACGCTGCGCAACAACCTGCGTCTGGCT
GGATTCGGGGGAGAGGCGGTGGGGGAGGCCCTGATGGCCGCCGGTCTGCGGCCTGACGTGCGGGCGGAGGACGTGCCGCT
GGAAGACCTGCGGGTACTTGCGCGGCGCCTCGGCGTGCTACGTTAG

Upstream 100 bases:

>100_bases
GAGAGCGCGGCCAGGGTCAGTCATGCGGGGAGTATACTGACGCGCTCGGCGCCCCTTTCGTCTCCTGTGTCCCCTATGCG
GGTCGCCGCCAAGGATTCCC

Downstream 100 bases:

>100_bases
GAACCGCGTTTCAAGCGCCAATTTTGTTGTGCCCAGGTTCGTGCGAACAAGGGGCATCGCGGGGCCGCCCCACCCTGGCC
CGGAGGTTCTTAAGTGAAGT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTQSEPPSLPLYSPARVRDLLTRHGLRPTKSLGQNFLIDGNILRAIAQAGGAAPGVPVLEVGPGLGVLTRELAARGAHVT
ALEKDERLRPVLAETLAGQDVQVVWGDALEFDYASLPAGTRVIANLPYYITGPLLARFMQAPGIISATVLVQKEVAGRLA
ARPGEDNYGFLSALAALYGTVQHVRDVPKGAFLPAPDVTSSVVRLDFDRARPAPEPAFLKFVEAALHHRRKTLRNNLRLA
GFGGEAVGEALMAAGLRPDVRAEDVPLEDLRVLARRLGVLR

Sequences:

>Translated_281_residues
MTQSEPPSLPLYSPARVRDLLTRHGLRPTKSLGQNFLIDGNILRAIAQAGGAAPGVPVLEVGPGLGVLTRELAARGAHVT
ALEKDERLRPVLAETLAGQDVQVVWGDALEFDYASLPAGTRVIANLPYYITGPLLARFMQAPGIISATVLVQKEVAGRLA
ARPGEDNYGFLSALAALYGTVQHVRDVPKGAFLPAPDVTSSVVRLDFDRARPAPEPAFLKFVEAALHHRRKTLRNNLRLA
GFGGEAVGEALMAAGLRPDVRAEDVPLEDLRVLARRLGVLR
>Mature_280_residues
TQSEPPSLPLYSPARVRDLLTRHGLRPTKSLGQNFLIDGNILRAIAQAGGAAPGVPVLEVGPGLGVLTRELAARGAHVTA
LEKDERLRPVLAETLAGQDVQVVWGDALEFDYASLPAGTRVIANLPYYITGPLLARFMQAPGIISATVLVQKEVAGRLAA
RPGEDNYGFLSALAALYGTVQHVRDVPKGAFLPAPDVTSSVVRLDFDRARPAPEPAFLKFVEAALHHRRKTLRNNLRLAG
FGGEAVGEALMAAGLRPDVRAEDVPLEDLRVLARRLGVLR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=233, Percent_Identity=35.1931330472103, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI156415992, Length=289, Percent_Identity=29.0657439446367, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786236, Length=258, Percent_Identity=33.7209302325581, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25146882, Length=220, Percent_Identity=31.3636363636364, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=255, Percent_Identity=27.0588235294118, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=186, Percent_Identity=35.4838709677419, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=274, Percent_Identity=30.6569343065693, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21357273, Length=232, Percent_Identity=29.3103448275862, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_DEIGD (Q1IZ94)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_604609.1
- ProteinModelPortal:   Q1IZ94
- SMR:   Q1IZ94
- STRING:   Q1IZ94
- GeneID:   4058311
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_1143
- NMPDR:   fig|68909.1.peg.2098
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q1IZ94
- ProtClustDB:   PRK00274
- BioCyc:   DGEO319795:DGEO_1143-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30116; Mature: 29985

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 35-35 BINDING 37-37 BINDING 62-62 BINDING 83-83 BINDING 107-107 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSEPPSLPLYSPARVRDLLTRHGLRPTKSLGQNFLIDGNILRAIAQAGGAAPGVPVLE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCEEECHHHHHHHHHHCCCCCCCCEEE
VGPGLGVLTRELAARGAHVTALEKDERLRPVLAETLAGQDVQVVWGDALEFDYASLPAGT
ECCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
RVIANLPYYITGPLLARFMQAPGIISATVLVQKEVAGRLAARPGEDNYGFLSALAALYGT
HHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VQHVRDVPKGAFLPAPDVTSSVVRLDFDRARPAPEPAFLKFVEAALHHRRKTLRNNLRLA
HHHHHHCCCCCCCCCCCHHHHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
GFGGEAVGEALMAAGLRPDVRAEDVPLEDLRVLARRLGVLR
ECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQSEPPSLPLYSPARVRDLLTRHGLRPTKSLGQNFLIDGNILRAIAQAGGAAPGVPVLE
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCEEECHHHHHHHHHHCCCCCCCCEEE
VGPGLGVLTRELAARGAHVTALEKDERLRPVLAETLAGQDVQVVWGDALEFDYASLPAGT
ECCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
RVIANLPYYITGPLLARFMQAPGIISATVLVQKEVAGRLAARPGEDNYGFLSALAALYGT
HHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VQHVRDVPKGAFLPAPDVTSSVVRLDFDRARPAPEPAFLKFVEAALHHRRKTLRNNLRLA
HHHHHHCCCCCCCCCCCHHHHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
GFGGEAVGEALMAAGLRPDVRAEDVPLEDLRVLARRLGVLR
ECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA