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The map label for this gene is livM [H]

Identifier: 94984908

GI number: 94984908

Start: 857626

End: 858606

Strand: Reverse

Name: livM [H]

Synonym: Dgeo_0801

Alternate gene names: 94984908

Gene position: 858606-857626 (Counterclockwise)

Preceding gene: 94984909

Following gene: 94984907

Centisome position: 34.8

GC content: 65.95

Gene sequence:

>981_bases
ATGGAGAGCCGCGTCCTAGAACCGGCCCGGACAGTGGCGCGCCGCCCAGAGTTCACCGCGCGGGCGCTCCTGCCGCTGGG
CCTCTTCTTCCTGCTCGCCCTGGTCTTCCCGTTCCTGCCGCTGGGAGCGCGAGCGGAATACCTGCTTCAGATCGCCTTTT
TCACGCTGGTAGCGGGAGTTCTGGCGCTCTCGTGGGACATTCTGGCGCGCAGCGGGCAGGTCTCGCTCGCACACGCCGCC
TTCTACGGCCTGGGAGCCTATGGTTTCGCGCTGCTGGTCAAGGTGACCCCGTGGTTTGTCGCGATGCCGCTCTCGGCGCT
GATCGCAGCGCTGATCTCGCTGATTCTGGGGGCGGTCACCCTGCGCCTGAGCGGGATGTACTTCGCTATTGCCACGCTCG
CCTTTACCGAGGTCGTGCGGACGATCATCCAGAATCTGCCGGAGTCGGTGGCGGGCGGGGCAAACGGGCTGCTGGTGCCT
GCGCTGCTGGGCGGGAACGCCCGGGCACAGTATTTCCTGGCCTTCGGGGTGCTGCTGTTCACCGCGCTCGTGAGCCTCGC
CATCCGGCGCTCGCGGCTGCATCACGCCTTTGCCGCCATCCGGCAGGGCGAGGAGACGGCGCGGGTGTTGGGCGTCCAGG
TGGTGCGCTACAAGCTGCTCGCCTTTTTCATCTCCTCCTTTCTGGCTGCGCTGGGCGGCGTGCTGTACGCCGGGAAGACC
TTTTTCATCAATCCGCTCGAAACCTTTAGCCTCGCCAACTCGATCGCGCCCCTCACCACCTCCATTTTCGGCGGGCTGTA
CACCACGTTGGGGCCGATCCTGGGGGCGACGGTGCTGCGGGTGGCCGAAGAAGGGCTGCATGCCTCCGTCAAGAACGGCT
ATCTGATTGTGTACGGCCTGGTGCTGATGCTGAGCATCCTGTGGCTGCCGCGCGGGCTGATGGGTCTCTTGCGGCGCAGG
AAGCATGGAGGGGAGCTGTGA

Upstream 100 bases:

>100_bases
ACGTGCCGGGGGGCGGCGGCTGGAGCGACGCCGTCTTCTTCCTGCTGATCTTCGCCACGCTGGTGTTCCGGTCGTTCCGG
GGGGCCAGGTGACGCGGGAG

Downstream 100 bases:

>100_bases
CGGCGGGAGCGCTCCGCGAATCAGCGCCGGTGCTGCGCGCCGAGGGCCTCAGCAAGCGGTTCGGCGGCTTGCACGCTGTG
CAGAACGTGAGCTTCACGCA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MESRVLEPARTVARRPEFTARALLPLGLFFLLALVFPFLPLGARAEYLLQIAFFTLVAGVLALSWDILARSGQVSLAHAA
FYGLGAYGFALLVKVTPWFVAMPLSALIAALISLILGAVTLRLSGMYFAIATLAFTEVVRTIIQNLPESVAGGANGLLVP
ALLGGNARAQYFLAFGVLLFTALVSLAIRRSRLHHAFAAIRQGEETARVLGVQVVRYKLLAFFISSFLAALGGVLYAGKT
FFINPLETFSLANSIAPLTTSIFGGLYTTLGPILGATVLRVAEEGLHASVKNGYLIVYGLVLMLSILWLPRGLMGLLRRR
KHGGEL

Sequences:

>Translated_326_residues
MESRVLEPARTVARRPEFTARALLPLGLFFLLALVFPFLPLGARAEYLLQIAFFTLVAGVLALSWDILARSGQVSLAHAA
FYGLGAYGFALLVKVTPWFVAMPLSALIAALISLILGAVTLRLSGMYFAIATLAFTEVVRTIIQNLPESVAGGANGLLVP
ALLGGNARAQYFLAFGVLLFTALVSLAIRRSRLHHAFAAIRQGEETARVLGVQVVRYKLLAFFISSFLAALGGVLYAGKT
FFINPLETFSLANSIAPLTTSIFGGLYTTLGPILGATVLRVAEEGLHASVKNGYLIVYGLVLMLSILWLPRGLMGLLRRR
KHGGEL
>Mature_326_residues
MESRVLEPARTVARRPEFTARALLPLGLFFLLALVFPFLPLGARAEYLLQIAFFTLVAGVLALSWDILARSGQVSLAHAA
FYGLGAYGFALLVKVTPWFVAMPLSALIAALISLILGAVTLRLSGMYFAIATLAFTEVVRTIIQNLPESVAGGANGLLVP
ALLGGNARAQYFLAFGVLLFTALVSLAIRRSRLHHAFAAIRQGEETARVLGVQVVRYKLLAFFISSFLAALGGVLYAGKT
FFINPLETFSLANSIAPLTTSIFGGLYTTLGPILGATVLRVAEEGLHASVKNGYLIVYGLVLMLSILWLPRGLMGLLRRR
KHGGEL

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=310, Percent_Identity=31.6129032258064, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35033; Mature: 35033

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESRVLEPARTVARRPEFTARALLPLGLFFLLALVFPFLPLGARAEYLLQIAFFTLVAGV
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LALSWDILARSGQVSLAHAAFYGLGAYGFALLVKVTPWFVAMPLSALIAALISLILGAVT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLSGMYFAIATLAFTEVVRTIIQNLPESVAGGANGLLVPALLGGNARAQYFLAFGVLLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
TALVSLAIRRSRLHHAFAAIRQGEETARVLGVQVVRYKLLAFFISSFLAALGGVLYAGKT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
FFINPLETFSLANSIAPLTTSIFGGLYTTLGPILGATVLRVAEEGLHASVKNGYLIVYGL
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VLMLSILWLPRGLMGLLRRRKHGGEL
HHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MESRVLEPARTVARRPEFTARALLPLGLFFLLALVFPFLPLGARAEYLLQIAFFTLVAGV
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LALSWDILARSGQVSLAHAAFYGLGAYGFALLVKVTPWFVAMPLSALIAALISLILGAVT
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLSGMYFAIATLAFTEVVRTIIQNLPESVAGGANGLLVPALLGGNARAQYFLAFGVLLF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
TALVSLAIRRSRLHHAFAAIRQGEETARVLGVQVVRYKLLAFFISSFLAALGGVLYAGKT
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
FFINPLETFSLANSIAPLTTSIFGGLYTTLGPILGATVLRVAEEGLHASVKNGYLIVYGL
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VLMLSILWLPRGLMGLLRRRKHGGEL
HHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]