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The map label for this gene is truB

Identifier: 94984868

GI number: 94984868

Start: 825177

End: 826115

Strand: Reverse

Name: truB

Synonym: Dgeo_0761

Alternate gene names: 94984868

Gene position: 826115-825177 (Counterclockwise)

Preceding gene: 94984871

Following gene: 94984864

Centisome position: 33.48

GC content: 70.29

Gene sequence:

>939_bases
ATGCCAGTCATCCCTGTGGACAAACCCTTGGGCCTAACCTCTCACGACGTGGTGAACCGGGCGCGCCGCGCACGCGGGAC
GCGGCGAGTCGGCCACACCGGTACCCTGGACCCGCTTGCTACCGGGGTGCTTGTGCTGTGTGTGGACGACTCCACCAAAC
TGGTGCAGTTCATGGAGGCAGACAGCAAGGAGTACCTCGCCTGGATTGCCCTGGGCGGCACCTCCCCCACCCTCGACGCC
GAGGGACCCCTGACCGAGGTCGTGCCGGTTTCCCCCCCCTCCGAGGAGGAGGCGCGGGCCGTGCTGGCGGGGTTCGTGGG
GCCACAGGCACAGGTGCCGCCGCAATACAGCGCAATCCAGGTCGGGGGACAGCGGGCCTACGCGGTAGCGCGGGCCGGAG
GCGCGCTGGACCTGCCCGCGCGGAACATCGTCATTCACGCGTTAGAGCTGCTCGGCATCTCCAACCGGATGGCGGAGTCT
CCCCGCACCTTCGCGCGTAGCTCCGAGGGCTGGACACCAGACCCAACCGGACGCACCTTCACCCTGCCCGAACCACTGGG
CGAGTTTCCCACCCTGCTCGTCCGCGCCAGCGTCAGCAGCGGCACCTACCTGCGCTCGCTCGCGCGGGACGTGGGAGCAG
CGCTGGGCGTGCCCGCACACCTGGCTGGCCTCGTCCGCACCCGGGTTGGCCGCTACGATCTGGCCGACAGTGTGAGCGTG
GAAGACCTGGCGGGGGCAGAGGGTTTCCCCAACCTCGCCGCCCTGGACCTGCCCAGGATGGAGGCCGATGAGCCGCTGGC
CCGCGCACTGCGGCAGGGCAAACGCCCAAGGTCGGCGGTGGTGGGGCGGCACGTCGTCACCCGGGACGGTGAGTTGGTCG
CGGTGGTGGACGGCGACGGGAAAGAACTGCGGGTGGTGCGGGCCTGGGCGGGGGCATAA

Upstream 100 bases:

>100_bases
GGCAGGCAAGAAGCGGGCTGAAGAACACTCGGAGGAGGTGACGCGCGGCCCTCAGCTTGGCTGACCGACACTGCCTCCGG
CTGCCCTACACTGCCTCCCG

Downstream 100 bases:

>100_bases
GCGGAAGCCCGTCAACAGCCCCCTCAGGATGCCGGGGCCACCTCCATCCGTACTGCTCCGATGCCTTCTAGCCCGCCTGC
CAGCGCCACGAGCCGCTCGT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPVIPVDKPLGLTSHDVVNRARRARGTRRVGHTGTLDPLATGVLVLCVDDSTKLVQFMEADSKEYLAWIALGGTSPTLDA
EGPLTEVVPVSPPSEEEARAVLAGFVGPQAQVPPQYSAIQVGGQRAYAVARAGGALDLPARNIVIHALELLGISNRMAES
PRTFARSSEGWTPDPTGRTFTLPEPLGEFPTLLVRASVSSGTYLRSLARDVGAALGVPAHLAGLVRTRVGRYDLADSVSV
EDLAGAEGFPNLAALDLPRMEADEPLARALRQGKRPRSAVVGRHVVTRDGELVAVVDGDGKELRVVRAWAGA

Sequences:

>Translated_312_residues
MPVIPVDKPLGLTSHDVVNRARRARGTRRVGHTGTLDPLATGVLVLCVDDSTKLVQFMEADSKEYLAWIALGGTSPTLDA
EGPLTEVVPVSPPSEEEARAVLAGFVGPQAQVPPQYSAIQVGGQRAYAVARAGGALDLPARNIVIHALELLGISNRMAES
PRTFARSSEGWTPDPTGRTFTLPEPLGEFPTLLVRASVSSGTYLRSLARDVGAALGVPAHLAGLVRTRVGRYDLADSVSV
EDLAGAEGFPNLAALDLPRMEADEPLARALRQGKRPRSAVVGRHVVTRDGELVAVVDGDGKELRVVRAWAGA
>Mature_311_residues
PVIPVDKPLGLTSHDVVNRARRARGTRRVGHTGTLDPLATGVLVLCVDDSTKLVQFMEADSKEYLAWIALGGTSPTLDAE
GPLTEVVPVSPPSEEEARAVLAGFVGPQAQVPPQYSAIQVGGQRAYAVARAGGALDLPARNIVIHALELLGISNRMAESP
RTFARSSEGWTPDPTGRTFTLPEPLGEFPTLLVRASVSSGTYLRSLARDVGAALGVPAHLAGLVRTRVGRYDLADSVSVE
DLAGAEGFPNLAALDLPRMEADEPLARALRQGKRPRSAVVGRHVVTRDGELVAVVDGDGKELRVVRAWAGA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=290, Percent_Identity=29.6551724137931, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI2367200, Length=264, Percent_Identity=32.1969696969697, Blast_Score=120, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6324037, Length=258, Percent_Identity=25.968992248062, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=284, Percent_Identity=27.4647887323944, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_DEIGD (Q1J0C1)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_604232.1
- ProteinModelPortal:   Q1J0C1
- SMR:   Q1J0C1
- STRING:   Q1J0C1
- GeneID:   4058616
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0761
- NMPDR:   fig|68909.1.peg.2574
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   NIVIHAL
- PhylomeDB:   Q1J0C1
- ProtClustDB:   PRK14122
- BioCyc:   DGEO319795:DGEO_0761-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 32948; Mature: 32817

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: ACT_SITE 37-37

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIPVDKPLGLTSHDVVNRARRARGTRRVGHTGTLDPLATGVLVLCVDDSTKLVQFMEA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEEEECCCHHHHHHHHC
DSKEYLAWIALGGTSPTLDAEGPLTEVVPVSPPSEEEARAVLAGFVGPQAQVPPQYSAIQ
CCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
VGGQRAYAVARAGGALDLPARNIVIHALELLGISNRMAESPRTFARSSEGWTPDPTGRTF
ECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCCCCCCCEE
TLPEPLGEFPTLLVRASVSSGTYLRSLARDVGAALGVPAHLAGLVRTRVGRYDLADSVSV
ECCCCCCHHCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCH
EDLAGAEGFPNLAALDLPRMEADEPLARALRQGKRPRSAVVGRHVVTRDGELVAVVDGDG
HHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCC
KELRVVRAWAGA
CEEEEEEEECCC
>Mature Secondary Structure 
PVIPVDKPLGLTSHDVVNRARRARGTRRVGHTGTLDPLATGVLVLCVDDSTKLVQFMEA
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEEEECCCHHHHHHHHC
DSKEYLAWIALGGTSPTLDAEGPLTEVVPVSPPSEEEARAVLAGFVGPQAQVPPQYSAIQ
CCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
VGGQRAYAVARAGGALDLPARNIVIHALELLGISNRMAESPRTFARSSEGWTPDPTGRTF
ECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCCCCCCCCEE
TLPEPLGEFPTLLVRASVSSGTYLRSLARDVGAALGVPAHLAGLVRTRVGRYDLADSVSV
ECCCCCCHHCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCH
EDLAGAEGFPNLAALDLPRMEADEPLARALRQGKRPRSAVVGRHVVTRDGELVAVVDGDG
HHHCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEEECCC
KELRVVRAWAGA
CEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA