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The map label for this gene is purR [H]

Identifier: 94984859

GI number: 94984859

Start: 814751

End: 815746

Strand: Reverse

Name: purR [H]

Synonym: Dgeo_0752

Alternate gene names: 94984859

Gene position: 815746-814751 (Counterclockwise)

Preceding gene: 94984860

Following gene: 94984858

Centisome position: 33.06

GC content: 70.68

Gene sequence:

>996_bases
ATGATCAAAGCCCAACCACGCCCCACCATCGACGACATTGCCCGCGCCAGCGGCGTGAGCAAGGGCACCGTGAGCCGCGT
GATCAACGGGCATCCCACGGTCGCGGCGGCCACCCGCGAACGGGTTCAGGCGGTGATGGAGCAGTTGGGGTACACCCCCG
ACCCCGCCGCGCGGCACCTGTCGTGGCGCAAGGGGCAGACGCTGGGCCTCTCGCTGGACCGCGACGACCCGATGCTCAGC
CCCTACCACGTGCTGTTTCGCCGCGCGCTGGAGCAGCACACCGGGCCGCAGGGGGTGCAGCTCGTCGATCTGCGCGCCGA
CCTCACCCAGCTCACCCGGCTCCCCAGCGCCGTGCTGGTCCTGCATGCCGTGGACGGCGATTCGCGGCTGGCCTACCTGC
AGCAGCAGGGTGTGCCTGCTGTGCTGATCGGTCATCAGCCCGGTGCCTTTTGGGTGGCTCCCGACGACGTGCGGGGCGCG
GCGCTGGCTGCCCGGACCCTGACGGACGCTGGGCACCGCCGTCTTGCCTACCTCGGGGTCGGCCCCAGTCAGGTAGCGCA
GGACCGCCAACGCGGGTTCGAACAGGCGGCCCGGCAGGCCGGAGCCGAGGTCGTGCCCATTCCGGCAGACTTCACCGTGC
TGGGCGGCTACCGGGCGATGCGCCGCGCCTGGGAGGAGGGAACGCGCTTTAGCGGCTTCTTTGCCCAGAGTGACGAGAGT
GCGGTGGGCGCAATCGCCGCGCTGGAGGATCTAGGCGTGCGGGTGCCGGAAGACGTGTCGGCGGTGGGCTTCGACGGCCT
GCCCGAGTTGCCTCTCCCCTACCCGCTGACCACCGTCGCGCAGGACATTCCCCGGATCGCCGCCACCGCGCTTCAGCTGA
TGCAGGAAGCCATCGCCGGTCAGGAACCGCGTGGCGAATTCGTTCCCGTCACCCTTCTTCCCGGCGCGACCGTCTCTAGG
CCGAGCGGCGCGCCCCTCCCTGGAGGTCCCGTATGA

Upstream 100 bases:

>100_bases
TGGGAAGAATGGGATTGTAACCGCTTACAACTCTTCGGCGGCGCTGGAGGCCGTTTGATTTGGTCGGCATTGTAACCGGT
TACAGAAGCGGCGAACCACG

Downstream 100 bases:

>100_bases
AAAACCTGGCCCTGCTCAGTCTCGCCGTCCTCGCCTCGGGTCTGCTGTCCAGCGCGGGCGCGCAGACCACCATCCGCATC
AACGGCTACGGCGGCACTGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIKAQPRPTIDDIARASGVSKGTVSRVINGHPTVAAATRERVQAVMEQLGYTPDPAARHLSWRKGQTLGLSLDRDDPMLS
PYHVLFRRALEQHTGPQGVQLVDLRADLTQLTRLPSAVLVLHAVDGDSRLAYLQQQGVPAVLIGHQPGAFWVAPDDVRGA
ALAARTLTDAGHRRLAYLGVGPSQVAQDRQRGFEQAARQAGAEVVPIPADFTVLGGYRAMRRAWEEGTRFSGFFAQSDES
AVGAIAALEDLGVRVPEDVSAVGFDGLPELPLPYPLTTVAQDIPRIAATALQLMQEAIAGQEPRGEFVPVTLLPGATVSR
PSGAPLPGGPV

Sequences:

>Translated_331_residues
MIKAQPRPTIDDIARASGVSKGTVSRVINGHPTVAAATRERVQAVMEQLGYTPDPAARHLSWRKGQTLGLSLDRDDPMLS
PYHVLFRRALEQHTGPQGVQLVDLRADLTQLTRLPSAVLVLHAVDGDSRLAYLQQQGVPAVLIGHQPGAFWVAPDDVRGA
ALAARTLTDAGHRRLAYLGVGPSQVAQDRQRGFEQAARQAGAEVVPIPADFTVLGGYRAMRRAWEEGTRFSGFFAQSDES
AVGAIAALEDLGVRVPEDVSAVGFDGLPELPLPYPLTTVAQDIPRIAATALQLMQEAIAGQEPRGEFVPVTLLPGATVSR
PSGAPLPGGPV
>Mature_331_residues
MIKAQPRPTIDDIARASGVSKGTVSRVINGHPTVAAATRERVQAVMEQLGYTPDPAARHLSWRKGQTLGLSLDRDDPMLS
PYHVLFRRALEQHTGPQGVQLVDLRADLTQLTRLPSAVLVLHAVDGDSRLAYLQQQGVPAVLIGHQPGAFWVAPDDVRGA
ALAARTLTDAGHRRLAYLGVGPSQVAQDRQRGFEQAARQAGAEVVPIPADFTVLGGYRAMRRAWEEGTRFSGFFAQSDES
AVGAIAALEDLGVRVPEDVSAVGFDGLPELPLPYPLTTVAQDIPRIAATALQLMQEAIAGQEPRGEFVPVTLLPGATVSR
PSGAPLPGGPV

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=35, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=27.9279279279279, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789068, Length=347, Percent_Identity=29.1066282420749, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1788474, Length=344, Percent_Identity=28.7790697674419, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=28.5266457680251, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=28.4375, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI48994940, Length=278, Percent_Identity=28.0575539568345, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786540, Length=331, Percent_Identity=25.3776435045317, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=26.4615384615385, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787580, Length=282, Percent_Identity=24.113475177305, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790192, Length=257, Percent_Identity=24.5136186770428, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35218; Mature: 35218

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKAQPRPTIDDIARASGVSKGTVSRVINGHPTVAAATRERVQAVMEQLGYTPDPAARHL
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
SWRKGQTLGLSLDRDDPMLSPYHVLFRRALEQHTGPQGVQLVDLRADLTQLTRLPSAVLV
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCEEEE
LHAVDGDSRLAYLQQQGVPAVLIGHQPGAFWVAPDDVRGAALAARTLTDAGHRRLAYLGV
EEEECCCCHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEC
GPSQVAQDRQRGFEQAARQAGAEVVPIPADFTVLGGYRAMRRAWEEGTRFSGFFAQSDES
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHCCCCEECEECCCCCC
AVGAIAALEDLGVRVPEDVSAVGFDGLPELPLPYPLTTVAQDIPRIAATALQLMQEAIAG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QEPRGEFVPVTLLPGATVSRPSGAPLPGGPV
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKAQPRPTIDDIARASGVSKGTVSRVINGHPTVAAATRERVQAVMEQLGYTPDPAARHL
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
SWRKGQTLGLSLDRDDPMLSPYHVLFRRALEQHTGPQGVQLVDLRADLTQLTRLPSAVLV
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCEEEE
LHAVDGDSRLAYLQQQGVPAVLIGHQPGAFWVAPDDVRGAALAARTLTDAGHRRLAYLGV
EEEECCCCHHHHHHHCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEC
GPSQVAQDRQRGFEQAARQAGAEVVPIPADFTVLGGYRAMRRAWEEGTRFSGFFAQSDES
CHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHCCCCEECEECCCCCC
AVGAIAALEDLGVRVPEDVSAVGFDGLPELPLPYPLTTVAQDIPRIAATALQLMQEAIAG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QEPRGEFVPVTLLPGATVSRPSGAPLPGGPV
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA