Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 94984840

GI number: 94984840

Start: 793880

End: 794821

Strand: Reverse

Name: braE [H]

Synonym: Dgeo_0733

Alternate gene names: 94984840

Gene position: 794821-793880 (Counterclockwise)

Preceding gene: 94984841

Following gene: 94984839

Centisome position: 32.22

GC content: 66.03

Gene sequence:

>942_bases
ATGAGTGGATTCCTCGAGCAATACGGCTTTCTGATCGTCACGATGGTGCAGGCGGGGCTGCTGGGCCTCAGCCTCTATTT
TCCCCTCCAGGCGGGGCAGCTCAGTCTGGCAAGCCCCGGCTTCTATGCTCTGGGCGGCTACGTTGCGGCGATTCTGTTGA
CGAATCCAGCGTTCTCGGGCCTGCGCGACACGCTGGGCAACGGCATGTTCCCGCTGACCTGGCTGGTTGCGGCCCTCCTC
TGCGGGCTGTTGGGCCTGTTTGTCGGTGTGCCCGCGCTGCGGCTCCGCGGCATCTACCTCGCGCTCGCCACGATCGCCTT
TGTGGAAATCCTGCGGGTGGTCAGCCTCAACCTTGCGGTCACAGGCGGCGCCATCGGCATCTTCGGAATTCCGCAGGCCT
TCGGCTTTCAGGACCGCTGGCAGTACCTCTGGCTGTTTGGGCCGCTGCTGCTCCTCACGCTGCTGTTTGCGCGGCAACTC
GAGCGCTCGCGGGTTGGGCGGGCCTTCCGCGCCATTCGCGAGGACGAACTTGCTGCCGACGCGATGGGCGTGCCTCCGAC
GCGCTACAAAGTGCTCGCCTTTGTGATCGGTGCGGTGCTGGCGGGCATTGTGGGGGCGATGAGTGCGCCCTTCCTGAACA
CCTGGAACGCCAAACAGGGCACCTTCGATGCCTCGATCGCCATCCTGGCCTTTGTCCTGATTGGCGGTTCGCGCAACATC
TGGGGACCGGTGGTGGGCGGCGCGCTGCTGACGGCCATTCCCGAAGTGCTGCGTTTCCTGGCCGACTGGCGGCTTATCAT
CAACGGCCTGGTGCTTGTCGTGGCGAGCCTCTATCTGCCGCAAGGCATCGTGGGAGCACTTCAGGGCGTGCGGCGTCCGC
CCCCACCCCGCCGTCCGCCTTCCGTGCCGGCCGTGGTGGAAGCCCCAGGGGGTGGCGCGTGA

Upstream 100 bases:

>100_bases
AGCGCGTACAAAGATGCCGTCGCCTTCGCGCTCCTGTTCGTGATTCTCCTGGTGCGCCCGCAGGGGCTGCTGGGACGCGC
GCAGATTCAAAAGGTCTGAG

Downstream 100 bases:

>100_bases
CCGCCATGCCCACCGAACGGGCAACCGTGCTGGAGGCGCGCGGGCTGACCCGGCGCTTTGGCGGCCTGGTCGCCGTGAAT
GATGTCTCCTTCGAGGTACG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSGFLEQYGFLIVTMVQAGLLGLSLYFPLQAGQLSLASPGFYALGGYVAAILLTNPAFSGLRDTLGNGMFPLTWLVAALL
CGLLGLFVGVPALRLRGIYLALATIAFVEILRVVSLNLAVTGGAIGIFGIPQAFGFQDRWQYLWLFGPLLLLTLLFARQL
ERSRVGRAFRAIREDELAADAMGVPPTRYKVLAFVIGAVLAGIVGAMSAPFLNTWNAKQGTFDASIAILAFVLIGGSRNI
WGPVVGGALLTAIPEVLRFLADWRLIINGLVLVVASLYLPQGIVGALQGVRRPPPPRRPPSVPAVVEAPGGGA

Sequences:

>Translated_313_residues
MSGFLEQYGFLIVTMVQAGLLGLSLYFPLQAGQLSLASPGFYALGGYVAAILLTNPAFSGLRDTLGNGMFPLTWLVAALL
CGLLGLFVGVPALRLRGIYLALATIAFVEILRVVSLNLAVTGGAIGIFGIPQAFGFQDRWQYLWLFGPLLLLTLLFARQL
ERSRVGRAFRAIREDELAADAMGVPPTRYKVLAFVIGAVLAGIVGAMSAPFLNTWNAKQGTFDASIAILAFVLIGGSRNI
WGPVVGGALLTAIPEVLRFLADWRLIINGLVLVVASLYLPQGIVGALQGVRRPPPPRRPPSVPAVVEAPGGGA
>Mature_312_residues
SGFLEQYGFLIVTMVQAGLLGLSLYFPLQAGQLSLASPGFYALGGYVAAILLTNPAFSGLRDTLGNGMFPLTWLVAALLC
GLLGLFVGVPALRLRGIYLALATIAFVEILRVVSLNLAVTGGAIGIFGIPQAFGFQDRWQYLWLFGPLLLLTLLFARQLE
RSRVGRAFRAIREDELAADAMGVPPTRYKVLAFVIGAVLAGIVGAMSAPFLNTWNAKQGTFDASIAILAFVLIGGSRNIW
GPVVGGALLTAIPEVLRFLADWRLIINGLVLVVASLYLPQGIVGALQGVRRPPPPRRPPSVPAVVEAPGGGA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=282, Percent_Identity=31.2056737588652, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33140; Mature: 33009

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFLEQYGFLIVTMVQAGLLGLSLYFPLQAGQLSLASPGFYALGGYVAAILLTNPAFSG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHCCHHHH
LRDTLGNGMFPLTWLVAALLCGLLGLFVGVPALRLRGIYLALATIAFVEILRVVSLNLAV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGGAIGIFGIPQAFGFQDRWQYLWLFGPLLLLTLLFARQLERSRVGRAFRAIREDELAAD
ECCCEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMGVPPTRYKVLAFVIGAVLAGIVGAMSAPFLNTWNAKQGTFDASIAILAFVLIGGSRNI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
WGPVVGGALLTAIPEVLRFLADWRLIINGLVLVVASLYLPQGIVGALQGVRRPPPPRRPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCC
SVPAVVEAPGGGA
CCCEEEECCCCCC
>Mature Secondary Structure 
SGFLEQYGFLIVTMVQAGLLGLSLYFPLQAGQLSLASPGFYALGGYVAAILLTNPAFSG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHCCHHHH
LRDTLGNGMFPLTWLVAALLCGLLGLFVGVPALRLRGIYLALATIAFVEILRVVSLNLAV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
TGGAIGIFGIPQAFGFQDRWQYLWLFGPLLLLTLLFARQLERSRVGRAFRAIREDELAAD
ECCCEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMGVPPTRYKVLAFVIGAVLAGIVGAMSAPFLNTWNAKQGTFDASIAILAFVLIGGSRNI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
WGPVVGGALLTAIPEVLRFLADWRLIINGLVLVVASLYLPQGIVGALQGVRRPPPPRRPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCC
SVPAVVEAPGGGA
CCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]