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The map label for this gene is purB [H]

Identifier: 94984795

GI number: 94984795

Start: 746322

End: 747629

Strand: Reverse

Name: purB [H]

Synonym: Dgeo_0688

Alternate gene names: 94984795

Gene position: 747629-746322 (Counterclockwise)

Preceding gene: 94984796

Following gene: 94984794

Centisome position: 30.3

GC content: 67.51

Gene sequence:

>1308_bases
GTGATCGACCGTTACCTGACCCCTGAGATGAAGCTGCTGTGGAGCGAGGCGAGCCGGTACCGCGCGTGGCTCAAGGTGGA
GCTGGCGGCCATGGAGGCGCAGGCCCGGCAAGGCGAGGTACCCCGCGAGGCGTTCGAGGCCCTGAGCACCCGCGCCGCCG
CCGACCCGCTCGACGAGGCGTTTGCCGTGCGGGTCGCGGAGATTGAGGCAGTGACGCGACACGACATCGTCGCCTTTACC
CGCGCCCTGACCGAACGCTACGGCGAGGAGGCGCGCTTCATTCATCACGGCCTGACGAGTACAGACGTGGTGGACACCGC
CCAGAACCTGCTGCTGGACGAGGCGCTGAACATCATTCAGGAGGACGTGCGGGCGCTGCGCGAGGTCTGCCGGACCCAGG
CCGTCACACACAAGCACACGCCGGTTGTGGGCCGCACCCACGGCATCCACGCTGAGCCGATGACCTTTGGCCTGAAGTTC
CTGAACTGGATGGCGACCCTGGACCGCGACCTGGAGCGCCTGCACGCCGCCCGCGAACGCATCCGGGTCGTGATGCTGTC
GGGATCGGTGGGCACCTACGCCCACATTTCCCCCCGCATCGAGGAGGAGGTCGCCGCCGCCTGGGGCTGGCAGCCCGCAC
CGATCACCAACCAGACATTGGCCCGCGACCGCCACGCGGAAGTGCTCGCGGCGCTGGCCATCCTGGGAACCACGCTGGAA
AAGATAGCGGTGGAAATCCGCCACCTCCAGCGCAGCGAGGTCCGCGAGGCGATGGAGCCTTTCGGGCAGGGACAGACCGG
CAGTTCCTCCATGCCCCACAAGAAAAACCCAATCCTGACCGAGAACGTAACGGGGTTGAGTCGCCTCCTGCGCGGCCATC
TGGTGACGGGGCTAGAAAACGTGGCGCTGTGGCACGAACGCGACATCAGCCACTCCAGCGCCGAGCGGGTGATTCTGCCT
GACGCGACCGCTGCCGCGAGCTACGCCACCCGCCGCCTCACCGGGGTGCTGCGTGATCTGGTGGTCTTCCCCGAACGCAT
GCTGCGCAACCTGAACGACCTGGGCGGCCTGGTCTTCAGCCAGCGCGTGCTGCATGCCCTGATCGATGAGAAGGGGCTCT
CGCGCGAAGCGGCCTACGACCTGGTGCAGCGCAACGCCCTGCGCAGCTGGGAGACCGGCGAGGGCCTGCGTGACCTGCTG
CGCGCGGACCCGACCAACCCCCTCAGCGAGGCAGAATTGGACGCCGCCTTTGACCTGGGGTGGTACCTGCGGCATGTGGA
AGACATCTACGCGCGCTTTGGGCTGTGA

Upstream 100 bases:

>100_bases
GCCTGCACGGCGGGCAGGGCTTCCATCACCAGCGTGAGGACCTGCTGCAGGTCAAGCATGCAGCGAGTGGAGTGGAGGAA
CAGATGGTACGCTGCCCGCC

Downstream 100 bases:

>100_bases
GCACCGAGTGGACCGCTGCATGAGACCGGTCGCGGCTTCTCTCAGCCGGCGGCAGATTCCGCACGCTAGAGTTTGCTTTG
CACCTCACATTCCGCCCGAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIDRYLTPEMKLLWSEASRYRAWLKVELAAMEAQARQGEVPREAFEALSTRAAADPLDEAFAVRVAEIEAVTRHDIVAFT
RALTERYGEEARFIHHGLTSTDVVDTAQNLLLDEALNIIQEDVRALREVCRTQAVTHKHTPVVGRTHGIHAEPMTFGLKF
LNWMATLDRDLERLHAARERIRVVMLSGSVGTYAHISPRIEEEVAAAWGWQPAPITNQTLARDRHAEVLAALAILGTTLE
KIAVEIRHLQRSEVREAMEPFGQGQTGSSSMPHKKNPILTENVTGLSRLLRGHLVTGLENVALWHERDISHSSAERVILP
DATAAASYATRRLTGVLRDLVVFPERMLRNLNDLGGLVFSQRVLHALIDEKGLSREAAYDLVQRNALRSWETGEGLRDLL
RADPTNPLSEAELDAAFDLGWYLRHVEDIYARFGL

Sequences:

>Translated_435_residues
MIDRYLTPEMKLLWSEASRYRAWLKVELAAMEAQARQGEVPREAFEALSTRAAADPLDEAFAVRVAEIEAVTRHDIVAFT
RALTERYGEEARFIHHGLTSTDVVDTAQNLLLDEALNIIQEDVRALREVCRTQAVTHKHTPVVGRTHGIHAEPMTFGLKF
LNWMATLDRDLERLHAARERIRVVMLSGSVGTYAHISPRIEEEVAAAWGWQPAPITNQTLARDRHAEVLAALAILGTTLE
KIAVEIRHLQRSEVREAMEPFGQGQTGSSSMPHKKNPILTENVTGLSRLLRGHLVTGLENVALWHERDISHSSAERVILP
DATAAASYATRRLTGVLRDLVVFPERMLRNLNDLGGLVFSQRVLHALIDEKGLSREAAYDLVQRNALRSWETGEGLRDLL
RADPTNPLSEAELDAAFDLGWYLRHVEDIYARFGL
>Mature_435_residues
MIDRYLTPEMKLLWSEASRYRAWLKVELAAMEAQARQGEVPREAFEALSTRAAADPLDEAFAVRVAEIEAVTRHDIVAFT
RALTERYGEEARFIHHGLTSTDVVDTAQNLLLDEALNIIQEDVRALREVCRTQAVTHKHTPVVGRTHGIHAEPMTFGLKF
LNWMATLDRDLERLHAARERIRVVMLSGSVGTYAHISPRIEEEVAAAWGWQPAPITNQTLARDRHAEVLAALAILGTTLE
KIAVEIRHLQRSEVREAMEPFGQGQTGSSSMPHKKNPILTENVTGLSRLLRGHLVTGLENVALWHERDISHSSAERVILP
DATAAASYATRRLTGVLRDLVVFPERMLRNLNDLGGLVFSQRVLHALIDEKGLSREAAYDLVQRNALRSWETGEGLRDLL
RADPTNPLSEAELDAAFDLGWYLRHVEDIYARFGL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=392, Percent_Identity=27.8061224489796, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.0839895013123, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1787376, Length=325, Percent_Identity=29.8461538461538, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17508577, Length=404, Percent_Identity=25.7425742574257, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=336, Percent_Identity=27.3809523809524, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=394, Percent_Identity=28.9340101522843, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24647570, Length=457, Percent_Identity=26.9146608315098, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI78710009, Length=214, Percent_Identity=30.3738317757009, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640177, Length=214, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640179, Length=214, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48849; Mature: 48849

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRYLTPEMKLLWSEASRYRAWLKVELAAMEAQARQGEVPREAFEALSTRAAADPLDEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
FAVRVAEIEAVTRHDIVAFTRALTERYGEEARFIHHGLTSTDVVDTAQNLLLDEALNIIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EDVRALREVCRTQAVTHKHTPVVGRTHGIHAEPMTFGLKFLNWMATLDRDLERLHAARER
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRVVMLSGSVGTYAHISPRIEEEVAAAWGWQPAPITNQTLARDRHAEVLAALAILGTTLE
HEEEEEECCCCCCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KIAVEIRHLQRSEVREAMEPFGQGQTGSSSMPHKKNPILTENVTGLSRLLRGHLVTGLEN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VALWHERDISHSSAERVILPDATAAASYATRRLTGVLRDLVVFPERMLRNLNDLGGLVFS
HHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVLHALIDEKGLSREAAYDLVQRNALRSWETGEGLRDLLRADPTNPLSEAELDAAFDLG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
WYLRHVEDIYARFGL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDRYLTPEMKLLWSEASRYRAWLKVELAAMEAQARQGEVPREAFEALSTRAAADPLDEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
FAVRVAEIEAVTRHDIVAFTRALTERYGEEARFIHHGLTSTDVVDTAQNLLLDEALNIIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EDVRALREVCRTQAVTHKHTPVVGRTHGIHAEPMTFGLKFLNWMATLDRDLERLHAARER
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRVVMLSGSVGTYAHISPRIEEEVAAAWGWQPAPITNQTLARDRHAEVLAALAILGTTLE
HEEEEEECCCCCCEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KIAVEIRHLQRSEVREAMEPFGQGQTGSSSMPHKKNPILTENVTGLSRLLRGHLVTGLEN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VALWHERDISHSSAERVILPDATAAASYATRRLTGVLRDLVVFPERMLRNLNDLGGLVFS
HHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVLHALIDEKGLSREAAYDLVQRNALRSWETGEGLRDLLRADPTNPLSEAELDAAFDLG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
WYLRHVEDIYARFGL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]