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The map label for this gene is cca [H]

Identifier: 94984719

GI number: 94984719

Start: 654070

End: 655371

Strand: Reverse

Name: cca [H]

Synonym: Dgeo_0612

Alternate gene names: 94984719

Gene position: 655371-654070 (Counterclockwise)

Preceding gene: 94984720

Following gene: 94984718

Centisome position: 26.56

GC content: 71.35

Gene sequence:

>1302_bases
ATGTTCCGGCGCCGCCCTCCCCTCCCCCCCTTCCCGACGGGCGCGCTGCTGGTCGGCGGTGCTGCGCGCGACTGGCTGCG
GGGCGTGGCCCCCAAGGATTTCGACTGGGCAGTTCCCAAACCCGAGCAGGCGGCGGGCGTCCTGGCCGCGCAGGTGGGCG
GTTTGGCTTTCCCGCTGGACGAGGCACGCGGCTACTGGCGGGTGCACGTGCCGGGCGGCGTGCAACACGACTTCGTACCG
CTCCCCGCCGACGTGACGGACGATCTGCTGCGGCGCGACTTCACAGTCAATGCCATCGCCCTCACCGCGGACGGGCAGCT
GCTGGACCCGGCGGGTGGGCGGGACGATCTGCGTGCAAGGCGACTGCGCATGGTCTCAGAGATGAATCTGCGGGACGATC
CCTTGCGGGCGTGGCGGGCGGTGCGCTTTGAGGTGACGCTGGGGTTGCGTCTGGACCCAGCGACAGAGGCGGCGGTCCGG
CGGGTTGCGGCTGACCTCGCGGCGGGCCAGCTACAACTCCCCGCTCCGGAGCGGGTGCGCGATGAACTGCAAGCGCTCCT
GGCGCATCCGCAGGCGGCACCCAGCATTCTGCGACTGGAAGCCCTCGGCCTGCTGGCTCTCAGCCTGCCCGAGTTGCGGG
AGGGGATCGGCGTGCCGCAGGGCGGCTTTCACCATCTGGATGTGTTCGAACACGCGTTGGAAGCCCTACATCAGCTTCTG
GCCCGCTTTCCGGACTCCGACCTGCCGCTGCGCTGGGCCACACTGCTGCACGACGTGGGCAAGCCGCGGACACGCAACAC
CTCCGTTCGCCCCGACCGCACCACCTTTTACGGCCACGAGCGCGTGGGGGCAGAGCTGGCCCGGCAGGCCTTGACCCGGC
TGCGGCTGCCAGGCGCGGAAGTGGACCGGGTGGCGGCCCTGGTACGTGCCCATATGACGCCTCTGCCCACGAACGAGCGG
GAGGCGCGGCGCTTTGTTCACCGCCGCCGCGAGCTGCTGCCTGACCTCCTGCGGCTGATGCTCGCCGACCGAGAAGCCGC
ACGGGGACCGCAGAGCACGCCCGGCACCCGCCACGCCTACGCCCAAGGGATCGAGCGGGTGCTGGCCGCCTTGGAGGAGC
AACCCGCACCCCCACCTCCACTGCTCACAGGCCGGGACGTGATGGCGCTGCTGGAGGTGCCGCCCGGCCCCACCGTGGGG
GAGGCGCTGCGGGCTGTGGCTGAGGCGCAAGCGCTCGGGGAGGTGCGGGACGCGCAGGAGGCGCGGGCCTTTTTGCGCAC
GCGCTTTGGCCATAGCCCATAA

Upstream 100 bases:

>100_bases
CCCGCTGAGCTATGCCCCCTCAGCGCGCACAGGCTAGCAGAGGGAAGGAGCTGAGGCAAGGCTTGGCCAGCGACGCCCGC
CCAGCCGCTAGCATGGCGCT

Downstream 100 bases:

>100_bases
GGATCGGACAGCGCGCTTTCGCTATTCTGGCTCGGCATGCCGCCCTCTACACCCTCTTCCGAAGCACACCCTCTCACGCC
CGAATGGGTCGCGGACGCCG

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MFRRRPPLPPFPTGALLVGGAARDWLRGVAPKDFDWAVPKPEQAAGVLAAQVGGLAFPLDEARGYWRVHVPGGVQHDFVP
LPADVTDDLLRRDFTVNAIALTADGQLLDPAGGRDDLRARRLRMVSEMNLRDDPLRAWRAVRFEVTLGLRLDPATEAAVR
RVAADLAAGQLQLPAPERVRDELQALLAHPQAAPSILRLEALGLLALSLPELREGIGVPQGGFHHLDVFEHALEALHQLL
ARFPDSDLPLRWATLLHDVGKPRTRNTSVRPDRTTFYGHERVGAELARQALTRLRLPGAEVDRVAALVRAHMTPLPTNER
EARRFVHRRRELLPDLLRLMLADREAARGPQSTPGTRHAYAQGIERVLAALEEQPAPPPPLLTGRDVMALLEVPPGPTVG
EALRAVAEAQALGEVRDAQEARAFLRTRFGHSP

Sequences:

>Translated_433_residues
MFRRRPPLPPFPTGALLVGGAARDWLRGVAPKDFDWAVPKPEQAAGVLAAQVGGLAFPLDEARGYWRVHVPGGVQHDFVP
LPADVTDDLLRRDFTVNAIALTADGQLLDPAGGRDDLRARRLRMVSEMNLRDDPLRAWRAVRFEVTLGLRLDPATEAAVR
RVAADLAAGQLQLPAPERVRDELQALLAHPQAAPSILRLEALGLLALSLPELREGIGVPQGGFHHLDVFEHALEALHQLL
ARFPDSDLPLRWATLLHDVGKPRTRNTSVRPDRTTFYGHERVGAELARQALTRLRLPGAEVDRVAALVRAHMTPLPTNER
EARRFVHRRRELLPDLLRLMLADREAARGPQSTPGTRHAYAQGIERVLAALEEQPAPPPPLLTGRDVMALLEVPPGPTVG
EALRAVAEAQALGEVRDAQEARAFLRTRFGHSP
>Mature_433_residues
MFRRRPPLPPFPTGALLVGGAARDWLRGVAPKDFDWAVPKPEQAAGVLAAQVGGLAFPLDEARGYWRVHVPGGVQHDFVP
LPADVTDDLLRRDFTVNAIALTADGQLLDPAGGRDDLRARRLRMVSEMNLRDDPLRAWRAVRFEVTLGLRLDPATEAAVR
RVAADLAAGQLQLPAPERVRDELQALLAHPQAAPSILRLEALGLLALSLPELREGIGVPQGGFHHLDVFEHALEALHQLL
ARFPDSDLPLRWATLLHDVGKPRTRNTSVRPDRTTFYGHERVGAELARQALTRLRLPGAEVDRVAALVRAHMTPLPTNER
EARRFVHRRRELLPDLLRLMLADREAARGPQSTPGTRHAYAQGIERVLAALEEQPAPPPPLLTGRDVMALLEVPPGPTVG
EALRAVAEAQALGEVRDAQEARAFLRTRFGHSP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=354, Percent_Identity=33.6158192090396, Blast_Score=104, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47618; Mature: 47618

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRRPPLPPFPTGALLVGGAARDWLRGVAPKDFDWAVPKPEQAAGVLAAQVGGLAFPLD
CCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
EARGYWRVHVPGGVQHDFVPLPADVTDDLLRRDFTVNAIALTADGQLLDPAGGRDDLRAR
HCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCEECCCCCHHHHHHH
RLRMVSEMNLRDDPLRAWRAVRFEVTLGLRLDPATEAAVRRVAADLAAGQLQLPAPERVR
HHHHHHHCCCCCCHHHHHHHEEEEEEEEEEECCHHHHHHHHHHHHHHCCCEECCCHHHHH
DELQALLAHPQAAPSILRLEALGLLALSLPELREGIGVPQGGFHHLDVFEHALEALHQLL
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ARFPDSDLPLRWATLLHDVGKPRTRNTSVRPDRTTFYGHERVGAELARQALTRLRLPGAE
HHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCH
VDRVAALVRAHMTPLPTNEREARRFVHRRRELLPDLLRLMLADREAARGPQSTPGTRHAY
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AQGIERVLAALEEQPAPPPPLLTGRDVMALLEVPPGPTVGEALRAVAEAQALGEVRDAQE
HHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARAFLRTRFGHSP
HHHHHHHHCCCCC
>Mature Secondary Structure
MFRRRPPLPPFPTGALLVGGAARDWLRGVAPKDFDWAVPKPEQAAGVLAAQVGGLAFPLD
CCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
EARGYWRVHVPGGVQHDFVPLPADVTDDLLRRDFTVNAIALTADGQLLDPAGGRDDLRAR
HCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCEECCCCCHHHHHHH
RLRMVSEMNLRDDPLRAWRAVRFEVTLGLRLDPATEAAVRRVAADLAAGQLQLPAPERVR
HHHHHHHCCCCCCHHHHHHHEEEEEEEEEEECCHHHHHHHHHHHHHHCCCEECCCHHHHH
DELQALLAHPQAAPSILRLEALGLLALSLPELREGIGVPQGGFHHLDVFEHALEALHQLL
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ARFPDSDLPLRWATLLHDVGKPRTRNTSVRPDRTTFYGHERVGAELARQALTRLRLPGAE
HHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCH
VDRVAALVRAHMTPLPTNEREARRFVHRRRELLPDLLRLMLADREAARGPQSTPGTRHAY
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AQGIERVLAALEEQPAPPPPLLTGRDVMALLEVPPGPTVGEALRAVAEAQALGEVRDAQE
HHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ARAFLRTRFGHSP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA