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The map label for this gene is nplT [H]

Identifier: 94984718

GI number: 94984718

Start: 652543

End: 654033

Strand: Reverse

Name: nplT [H]

Synonym: Dgeo_0611

Alternate gene names: 94984718

Gene position: 654033-652543 (Counterclockwise)

Preceding gene: 94984719

Following gene: 94984716

Centisome position: 26.51

GC content: 66.87

Gene sequence:

>1491_bases
ATGCCGCCCTCTACACCCTCTTCCGAAGCACACCCTCTCACGCCCGAATGGGTCGCGGACGCCGTCTTCTATCAGATTTT
CCCCGACCGGTTCGCACGCAGCGGGCGAGTGCCGGGCCTGAACCTTCAGCCCTGGGGCAGCCCGCCGCAGGTGCACGGGT
ATATGGGCGGCGACCTGTGGGGGGTCACCCAGCATCTGGACCACATCGGGGCACTGGGGGTAAACGCGATCTACTTTTGC
CCGGTATTTCAGTCGGCTTCCAACCACCGCTACCACACCCACGACTACTACCGGGTAGATCCCATGCTGGGGGGCAACGA
GGCGCTGCGGGCGCTGATCGAGGCGGCGCATGCGCGGGGCATCCGCATCGTGCTGGACGGCGTCTTTAACCATGCCAGCC
GGGGCTTTTTTCAGTTCAACGACCTCTTGGAACAGGGCGAGGCGAGCGCCTACCGTGACTGGTTCCATGTGGAGGGATGG
CCCCTGCACGCCTACGACGACACCCGACCGGCGAACTATCAGGCGTGGTGGGGCCTTCGCGCGCTGCCGAAATTCAACAC
CGGCAACCCGGCCGTCCGCGAGTTCTTGTGGGACGTGGGCACGTATTGGATGGAGTTTGGGATTGACGGCTGGCGGCTCG
ACGTACCCAACGAAATCGATGACGACGAGTTCTGGCGCGAGTTCCGCCGCCGCGTGAAGGCCATCAATCCCGACGCCTAC
ATCGTGGGCGAAATCTGGGGCGACGCGCACCGCTGGCTTGCCGGGGACCAGTTCGACGCGGTGATGAATTATCACTTCAC
CCGGCCCTGTCTGGCGTTTTTTGCGGCGCGAACCCTCGCACAGGCGGCCAACGAGTCCACCGGCCTAGGCCGTGTGGAGC
CACTCGACGCTGCCGCCTTCGCCCGCCGCATGACCGAGATCACAGCGCTGTATCACCCCGAGATCGTCCGCGCGCAGCTC
AACCTGCTCGACTCACACGACACAGCCCGCTTCCTGACCGCCGCGGGCGGCGACGCGACCGCCTTTCGCCTCGCCAGCAT
CTTTCAGATGACCTACGTGGGGGCGCCGTGCATCTACTACGGTGATGAGATCGGTCTTCCCGGCGGCCCTGACCCCGACT
GCCGCCGCGCTTTCCCCTGGCACGACGAGGCGAGCTGGGACAAAGAGACGCTGAGCCTGCTGCAAAAGCTCACCGCCACC
CGCCACGCCACCCCCGCCCTGCGCCGGGGAGCATTCCGCTTCACCCACGCGGAAGGCGAGGCGCTGGTCTACGTCCGCGA
ACACGCCGCCGGCAACGCCTACATCGCCATCAATGCCGCCCAAGCCCCCGCCCGCCTGCCCCTCAGCGACGTGCAGCCCG
GCCGATACCGCGACGCCCTGAGCGGGCAGCGTCACGAGTTGAGCGGAGAAACGGAGGTCGAGGTTCCGGGGCGCGCGGCG
GTGGTGCTGGTCCGGGCGGAGGAGGATTCGCGGCACACCGCGCGGCACTGA

Upstream 100 bases:

>100_bases
GGAGGTGCGGGACGCGCAGGAGGCGCGGGCCTTTTTGCGCACGCGCTTTGGCCATAGCCCATAAGGATCGGACAGCGCGC
TTTCGCTATTCTGGCTCGGC

Downstream 100 bases:

>100_bases
CAACCGATGGTCTCAGTGCAGTGGTACGTGCCCCGGAAACCCGATCAGCCAGTCGAGGAGGTCGCCCACCATCGCGGAGG
CCGTGACCATGCCGCCCGCG

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MPPSTPSSEAHPLTPEWVADAVFYQIFPDRFARSGRVPGLNLQPWGSPPQVHGYMGGDLWGVTQHLDHIGALGVNAIYFC
PVFQSASNHRYHTHDYYRVDPMLGGNEALRALIEAAHARGIRIVLDGVFNHASRGFFQFNDLLEQGEASAYRDWFHVEGW
PLHAYDDTRPANYQAWWGLRALPKFNTGNPAVREFLWDVGTYWMEFGIDGWRLDVPNEIDDDEFWREFRRRVKAINPDAY
IVGEIWGDAHRWLAGDQFDAVMNYHFTRPCLAFFAARTLAQAANESTGLGRVEPLDAAAFARRMTEITALYHPEIVRAQL
NLLDSHDTARFLTAAGGDATAFRLASIFQMTYVGAPCIYYGDEIGLPGGPDPDCRRAFPWHDEASWDKETLSLLQKLTAT
RHATPALRRGAFRFTHAEGEALVYVREHAAGNAYIAINAAQAPARLPLSDVQPGRYRDALSGQRHELSGETEVEVPGRAA
VVLVRAEEDSRHTARH

Sequences:

>Translated_496_residues
MPPSTPSSEAHPLTPEWVADAVFYQIFPDRFARSGRVPGLNLQPWGSPPQVHGYMGGDLWGVTQHLDHIGALGVNAIYFC
PVFQSASNHRYHTHDYYRVDPMLGGNEALRALIEAAHARGIRIVLDGVFNHASRGFFQFNDLLEQGEASAYRDWFHVEGW
PLHAYDDTRPANYQAWWGLRALPKFNTGNPAVREFLWDVGTYWMEFGIDGWRLDVPNEIDDDEFWREFRRRVKAINPDAY
IVGEIWGDAHRWLAGDQFDAVMNYHFTRPCLAFFAARTLAQAANESTGLGRVEPLDAAAFARRMTEITALYHPEIVRAQL
NLLDSHDTARFLTAAGGDATAFRLASIFQMTYVGAPCIYYGDEIGLPGGPDPDCRRAFPWHDEASWDKETLSLLQKLTAT
RHATPALRRGAFRFTHAEGEALVYVREHAAGNAYIAINAAQAPARLPLSDVQPGRYRDALSGQRHELSGETEVEVPGRAA
VVLVRAEEDSRHTARH
>Mature_495_residues
PPSTPSSEAHPLTPEWVADAVFYQIFPDRFARSGRVPGLNLQPWGSPPQVHGYMGGDLWGVTQHLDHIGALGVNAIYFCP
VFQSASNHRYHTHDYYRVDPMLGGNEALRALIEAAHARGIRIVLDGVFNHASRGFFQFNDLLEQGEASAYRDWFHVEGWP
LHAYDDTRPANYQAWWGLRALPKFNTGNPAVREFLWDVGTYWMEFGIDGWRLDVPNEIDDDEFWREFRRRVKAINPDAYI
VGEIWGDAHRWLAGDQFDAVMNYHFTRPCLAFFAARTLAQAANESTGLGRVEPLDAAAFARRMTEITALYHPEIVRAQLN
LLDSHDTARFLTAAGGDATAFRLASIFQMTYVGAPCIYYGDEIGLPGGPDPDCRRAFPWHDEASWDKETLSLLQKLTATR
HATPALRRGAFRFTHAEGEALVYVREHAAGNAYIAINAAQAPARLPLSDVQPGRYRDALSGQRHELSGETEVEVPGRAAV
VLVRAEEDSRHTARH

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=395, Percent_Identity=21.2658227848101, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786604, Length=469, Percent_Identity=35.3944562899787, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1789995, Length=514, Percent_Identity=25.2918287937743, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790687, Length=229, Percent_Identity=33.1877729257642, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322245, Length=227, Percent_Identity=31.7180616740088, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=234, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322241, Length=233, Percent_Identity=30.0429184549356, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6322021, Length=233, Percent_Identity=30.0429184549356, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324416, Length=233, Percent_Identity=30.0429184549356, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321731, Length=229, Percent_Identity=28.82096069869, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319776, Length=229, Percent_Identity=28.82096069869, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24586587, Length=219, Percent_Identity=27.8538812785388, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586591, Length=414, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583747, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583749, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583745, Length=219, Percent_Identity=28.310502283105, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24586599, Length=228, Percent_Identity=28.5087719298246, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586589, Length=406, Percent_Identity=24.384236453202, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221330053, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586593, Length=531, Percent_Identity=23.728813559322, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586597, Length=222, Percent_Identity=29.2792792792793, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45549022, Length=537, Percent_Identity=21.415270018622, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 55722; Mature: 55591

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSTPSSEAHPLTPEWVADAVFYQIFPDRFARSGRVPGLNLQPWGSPPQVHGYMGGDLW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHH
GVTQHLDHIGALGVNAIYFCPVFQSASNHRYHTHDYYRVDPMLGGNEALRALIEAAHARG
HHHHHHHHHHHHCCCEEEEEECHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
IRIVLDGVFNHASRGFFQFNDLLEQGEASAYRDWFHVEGWPLHAYDDTRPANYQAWWGLR
EEEEEEHHHHHHHCCHHHHHHHHHCCCCHHHHCEEECCCEEEECCCCCCCCCCCCEECCC
ALPKFNTGNPAVREFLWDVGTYWMEFGIDGWRLDVPNEIDDDEFWREFRRRVKAINPDAY
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
IVGEIWGDAHRWLAGDQFDAVMNYHFTRPCLAFFAARTLAQAANESTGLGRVEPLDAAAF
EEEEECCCHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ARRMTEITALYHPEIVRAQLNLLDSHDTARFLTAAGGDATAFRLASIFQMTYVGAPCIYY
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
GDEIGLPGGPDPDCRRAFPWHDEASWDKETLSLLQKLTATRHATPALRRGAFRFTHAEGE
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCC
ALVYVREHAAGNAYIAINAAQAPARLPLSDVQPGRYRDALSGQRHELSGETEVEVPGRAA
EEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCEE
VVLVRAEEDSRHTARH
EEEEECCCCCCCCCCC
>Mature Secondary Structure 
PPSTPSSEAHPLTPEWVADAVFYQIFPDRFARSGRVPGLNLQPWGSPPQVHGYMGGDLW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCHH
GVTQHLDHIGALGVNAIYFCPVFQSASNHRYHTHDYYRVDPMLGGNEALRALIEAAHARG
HHHHHHHHHHHHCCCEEEEEECHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCC
IRIVLDGVFNHASRGFFQFNDLLEQGEASAYRDWFHVEGWPLHAYDDTRPANYQAWWGLR
EEEEEEHHHHHHHCCHHHHHHHHHCCCCHHHHCEEECCCEEEECCCCCCCCCCCCEECCC
ALPKFNTGNPAVREFLWDVGTYWMEFGIDGWRLDVPNEIDDDEFWREFRRRVKAINPDAY
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEE
IVGEIWGDAHRWLAGDQFDAVMNYHFTRPCLAFFAARTLAQAANESTGLGRVEPLDAAAF
EEEEECCCHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ARRMTEITALYHPEIVRAQLNLLDSHDTARFLTAAGGDATAFRLASIFQMTYVGAPCIYY
HHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEE
GDEIGLPGGPDPDCRRAFPWHDEASWDKETLSLLQKLTATRHATPALRRGAFRFTHAEGE
CCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEECCCC
ALVYVREHAAGNAYIAINAAQAPARLPLSDVQPGRYRDALSGQRHELSGETEVEVPGRAA
EEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCEE
VVLVRAEEDSRHTARH
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]