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The map label for this gene is prfA

Identifier: 94984716

GI number: 94984716

Start: 650323

End: 651435

Strand: Reverse

Name: prfA

Synonym: Dgeo_0609

Alternate gene names: 94984716

Gene position: 651435-650323 (Counterclockwise)

Preceding gene: 94984718

Following gene: 94984715

Centisome position: 26.4

GC content: 67.39

Gene sequence:

>1113_bases
GTGGTAAGCCAGCGACTGGAGGAACTGGCTGCCGAGTTCGGCCTGGTAGAGCGGGCACTGGGAGACCCAGCCATGCTGGC
GGATCCCCGCGAATACGCTCGGCTGACCCGCCGTCACCGCGAGCTCACGCCTATCGTGACCCTCTACCGCGAACATGCGG
TGCTCTCGAGCGATTTGGAGGGCGCGCGCGAGCTGCTGGCGGATCCCGATATGCGCGAACTGGCACAGGGCGAGATCGAA
AGTCTGAGCGCCCGCCTTGCCCAGATCGAGGCCGAGCTGGAGGTGCTGCTGCTGCCCACCGATCCCGACGACGCCAAGAA
CGTGATTCTGGAACTGCGTGCGGGTGCCGGAGGGGCGGAGGCGGCGCTCTTTGCCGTGGATCTGCTGCGCATGTACACCC
GTTACGCTGAGGGCGCGGGCCTAAAGCTCAACGTGCTGGACGCCTCAGAAAGTGACCTAGGCGGCGCGAGCAAGGTCGTG
GCGGAGGTGACGGGCGAATTTGCCTTCCGCGCCTTTAAGTGGGAACGCGGTGTTCACCGAGTCCAGCGCGTGCCCGCCAC
CGAATCGCAGGGCCGTATTCACACCAGCACCGTGACGGTGGCGGTGCTGCCCGAGGCCGAGCAGGGTGAGGTAAGCGTTG
ATCCCTCGGAGGTCCGCATCGACGTGTTCCGCTCGCAGGGGGCGGGCGGGCAGGGCGTCAACACCACCGACTCGGCGGTG
CGCGCAGTGTACCGGCCCGGCACGCCGGACGAGATCGTGGTGGTCTGCCAGGACAGCCGCTCACAGATCAAAAACCGCGA
GAAGGCGCTTGTCGTGCTGGCTTCACGTCTGGCCGAGCGGGAGCGGGCAGCGCGGGAGGAACGCGAACGCGAGACGCGCG
CCGCGCAGGTCGGCACCGGCGAACGCAGCGAGAAAATCCGCACCTACAACTATCCCCAGAACCGGGTGACCGATCACCGC
CTGGAAGGCGACGCCAAGAACTTCGCGCTCGACAGCGTGATGGCGGGCGGTCTCGCGCCGATTGTGGCGGCCTTGAGCCG
CGACGAGCGCGAACGGCAACTCCTGGAGCTGCAAGGGGCAGAAGGCGAACGGGGCACCTATGGCGCGGCGTGA

Upstream 100 bases:

>100_bases
CCCCTGTTGCCTGCCTTCCGGTCGTCTAGGGAGGGCGGCGCTAGACTGCGTTTATGATGCCTGTGTCAACTGTCCCCTTC
GTGTTTGGGTTGTGTCCGCC

Downstream 100 bases:

>100_bases
CCTGCTGGTGGCGGCAGGCGTGCTGCGGGATAAATTCGGGCGAGTGCTGCTGGTCGGGAACGACTGGCAGGGGTTGGGCC
GCGTGCGCCACACCCTGCCC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MVSQRLEELAAEFGLVERALGDPAMLADPREYARLTRRHRELTPIVTLYREHAVLSSDLEGARELLADPDMRELAQGEIE
SLSARLAQIEAELEVLLLPTDPDDAKNVILELRAGAGGAEAALFAVDLLRMYTRYAEGAGLKLNVLDASESDLGGASKVV
AEVTGEFAFRAFKWERGVHRVQRVPATESQGRIHTSTVTVAVLPEAEQGEVSVDPSEVRIDVFRSQGAGGQGVNTTDSAV
RAVYRPGTPDEIVVVCQDSRSQIKNREKALVVLASRLAERERAAREERERETRAAQVGTGERSEKIRTYNYPQNRVTDHR
LEGDAKNFALDSVMAGGLAPIVAALSRDERERQLLELQGAEGERGTYGAA

Sequences:

>Translated_370_residues
MVSQRLEELAAEFGLVERALGDPAMLADPREYARLTRRHRELTPIVTLYREHAVLSSDLEGARELLADPDMRELAQGEIE
SLSARLAQIEAELEVLLLPTDPDDAKNVILELRAGAGGAEAALFAVDLLRMYTRYAEGAGLKLNVLDASESDLGGASKVV
AEVTGEFAFRAFKWERGVHRVQRVPATESQGRIHTSTVTVAVLPEAEQGEVSVDPSEVRIDVFRSQGAGGQGVNTTDSAV
RAVYRPGTPDEIVVVCQDSRSQIKNREKALVVLASRLAERERAAREERERETRAAQVGTGERSEKIRTYNYPQNRVTDHR
LEGDAKNFALDSVMAGGLAPIVAALSRDERERQLLELQGAEGERGTYGAA
>Mature_370_residues
MVSQRLEELAAEFGLVERALGDPAMLADPREYARLTRRHRELTPIVTLYREHAVLSSDLEGARELLADPDMRELAQGEIE
SLSARLAQIEAELEVLLLPTDPDDAKNVILELRAGAGGAEAALFAVDLLRMYTRYAEGAGLKLNVLDASESDLGGASKVV
AEVTGEFAFRAFKWERGVHRVQRVPATESQGRIHTSTVTVAVLPEAEQGEVSVDPSEVRIDVFRSQGAGGQGVNTTDSAV
RAVYRPGTPDEIVVVCQDSRSQIKNREKALVVLASRLAERERAAREERERETRAAQVGTGERSEKIRTYNYPQNRVTDHR
LEGDAKNFALDSVMAGGLAPIVAALSRDERERQLLELQGAEGERGTYGAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=41.8300653594771, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=35.5300859598854, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI166795305, Length=216, Percent_Identity=42.5925925925926, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=46.045197740113, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI2367172, Length=347, Percent_Identity=32.2766570605187, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=39.1143911439114, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321295, Length=341, Percent_Identity=39.8826979472141, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI19921226, Length=335, Percent_Identity=40.5970149253731, Blast_Score=216, Evalue=1e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_DEIGD (Q1J0S3)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_604080.1
- ProteinModelPortal:   Q1J0S3
- SMR:   Q1J0S3
- STRING:   Q1J0S3
- GeneID:   4058059
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0609
- NMPDR:   fig|68909.1.peg.912
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   DGEO319795:DGEO_0609-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40482; Mature: 40482

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSQRLEELAAEFGLVERALGDPAMLADPREYARLTRRHRELTPIVTLYREHAVLSSDLE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GARELLADPDMRELAQGEIESLSARLAQIEAELEVLLLPTDPDDAKNVILELRAGAGGAE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEEEEEECCCCCHH
AALFAVDLLRMYTRYAEGAGLKLNVLDASESDLGGASKVVAEVTGEFAFRAFKWERGVHR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRVPATESQGRIHTSTVTVAVLPEAEQGEVSVDPSEVRIDVFRSQGAGGQGVNTTDSAV
HHHCCCCCCCCEEEEEEEEEEEECCCCCCCEECCHHHEEEEEEECCCCCCCCCCCHHHHH
RAVYRPGTPDEIVVVCQDSRSQIKNREKALVVLASRLAERERAAREERERETRAAQVGTG
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERSEKIRTYNYPQNRVTDHRLEGDAKNFALDSVMAGGLAPIVAALSRDERERQLLELQGA
CCCCCCEECCCCCCCCCCHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCC
EGERGTYGAA
CCCCCCCCCC
>Mature Secondary Structure
MVSQRLEELAAEFGLVERALGDPAMLADPREYARLTRRHRELTPIVTLYREHAVLSSDLE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GARELLADPDMRELAQGEIESLSARLAQIEAELEVLLLPTDPDDAKNVILELRAGAGGAE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEEEEEECCCCCHH
AALFAVDLLRMYTRYAEGAGLKLNVLDASESDLGGASKVVAEVTGEFAFRAFKWERGVHR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRVPATESQGRIHTSTVTVAVLPEAEQGEVSVDPSEVRIDVFRSQGAGGQGVNTTDSAV
HHHCCCCCCCCEEEEEEEEEEEECCCCCCCEECCHHHEEEEEEECCCCCCCCCCCHHHHH
RAVYRPGTPDEIVVVCQDSRSQIKNREKALVVLASRLAERERAAREERERETRAAQVGTG
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERSEKIRTYNYPQNRVTDHRLEGDAKNFALDSVMAGGLAPIVAALSRDERERQLLELQGA
CCCCCCEECCCCCCCCCCHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCC
EGERGTYGAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA