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The map label for this gene is leuA [H]

Identifier: 94984706

GI number: 94984706

Start: 639140

End: 640690

Strand: Reverse

Name: leuA [H]

Synonym: Dgeo_0599

Alternate gene names: 94984706

Gene position: 640690-639140 (Counterclockwise)

Preceding gene: 94984707

Following gene: 94984703

Centisome position: 25.97

GC content: 66.22

Gene sequence:

>1551_bases
ATGACTCAGCCCCAAGCCCAGGCCCAGCGCATCCGCATCTTCGACACCACCCTGCGTGACGGCGAGCAGTCGCCGGGTGT
GGCCCTGAATCACACCCAAAAGCTGGAGATCGCGCACCAGCTCGCTCGGCTGGGCGTCGACGTGATCGAGGCGGGCTTTC
CCATCGCCTCTCCCGGCGACCTGGAAGGCGTCTCGCGCATCGCCCGCGAGGTCCGCGGCCCCATCATCGCTGGGCTGGCT
CGCGCGGGCCGCGCCGACATCGAGGCGGCAGCCAGGGCGGTTGAGCTGGCGGAAAAGCCCCGCATCCACACCTTCATCGC
CACCAGCCCCATTCACATGGCCAAGAAACTGCAACTCGAACCGGACGCGGTGATCGAGCGAGCAGTGGAGGCGGTGCGGC
TGGCACGGTCCTTTGTGGACGACGTGGAATTCAGCGCAGAGGACGCCACCCGCAGCGACCGTGACTTCCTGGTGCGCATT
TTCCGCGCTGCGGTGGAGGCGGGTGCGACCACAATCAACGTGCCCGATACGGTGGGCTACACCACACCGGAAGAGATCCG
CGACCTGTTCGCCTACCTGCGCGGCGAGCTGCCGGACCACATTATTCTCTCGGCCCACTGTCACGATGACCTGGGGATGG
CTGTGGCCAACTCCATCGCCGCGGCGGAAGGCGGCGCGCGACAGATTGAGTGCACTGTCAACGGCATTGGCGAGCGCGCT
GGGAATGCCAGCCTGGAAGAGATTGTGATGGCCTTTCACACCCGCCGTGATCACTACGGCTTCGAGACGGGCATCCGCAC
CCGCGAGATCTACCGCACCAGCCGCATGGTGAGTCGCCTGAGCGGGATGCCCGTCCAGCCCAACAAGGCTGTGGTGGGCG
ACAATGCCTTTGCGCACGAGTCGGGCATCCACCAGGACGGCGTCATCAAGGCGCGCGAGACCTACGAGATCATGAACGCC
GAGCTGGTGGGGCGTGAGGCTGCCGTGCTGGTGATGGGCAAGCACTCGGGCCGTGCCGCCTTCCGCAAGGCGCTGACGGA
TTTGGGCTACGCGGTGGACGAGGAACGCCTCAAGCAGCTGTTTGCCCGCTTCAAGGACATGGCCGACCGCAAGGGACAGA
TCTACGCAGACGACCTGCGCGCCCTGGTGGAAAGCCGCAGCGACGTGCCGCAGACCTTTACGCTCGAGGGCTTCCAGATC
ACCTCCGGCATGAACATGACACCGGTCGCCTTTGTGCGTCTGCAGACGCCCGACGGCCCGGTGGATGCGACCGCACACGG
CGACGGCCCGGTGGAGGCCGCTTTTCAGGCGATCAACAAAATCACCGGCATCACGCCCACGCTGGAGAGCTACCGCATCC
AGGCCGTCACGGGCGGCGGCGACGCGCTGGGCGAGGTCAGCATCGGCGCGCGCTACGGCGAGACGACCCTGCACGGAACC
GGCGTGGCGACCGATGTGGTTGAAGCTTCTGCCCGCGCCTGGATTCGCATCGTGAATCAGGTGGTGGCGGGCATGGGCAA
GAGCCGGGCGGTGAGTCAGACAACAGTGTGA

Upstream 100 bases:

>100_bases
TCCGGTGGAGTGAAGGGCGCAAAACCGCCTTCCCCCCCGGAGGACGCCCCTCCGGGGTTTTTCGTTGCTGCCCCGTCCTT
CTCACCTTCAGGAGACGCCC

Downstream 100 bases:

>100_bases
TGGAGGCGGGAGGGCAGCAGCAAGCGCGGAGGCGCTGCGCTGTTCTCTCGCTGCTGGGGAAACCCTGGACGCCTCAGGAA
CACCGGGGCTCCTGGCCGGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTQPQAQAQRIRIFDTTLRDGEQSPGVALNHTQKLEIAHQLARLGVDVIEAGFPIASPGDLEGVSRIAREVRGPIIAGLA
RAGRADIEAAARAVELAEKPRIHTFIATSPIHMAKKLQLEPDAVIERAVEAVRLARSFVDDVEFSAEDATRSDRDFLVRI
FRAAVEAGATTINVPDTVGYTTPEEIRDLFAYLRGELPDHIILSAHCHDDLGMAVANSIAAAEGGARQIECTVNGIGERA
GNASLEEIVMAFHTRRDHYGFETGIRTREIYRTSRMVSRLSGMPVQPNKAVVGDNAFAHESGIHQDGVIKARETYEIMNA
ELVGREAAVLVMGKHSGRAAFRKALTDLGYAVDEERLKQLFARFKDMADRKGQIYADDLRALVESRSDVPQTFTLEGFQI
TSGMNMTPVAFVRLQTPDGPVDATAHGDGPVEAAFQAINKITGITPTLESYRIQAVTGGGDALGEVSIGARYGETTLHGT
GVATDVVEASARAWIRIVNQVVAGMGKSRAVSQTTV

Sequences:

>Translated_516_residues
MTQPQAQAQRIRIFDTTLRDGEQSPGVALNHTQKLEIAHQLARLGVDVIEAGFPIASPGDLEGVSRIAREVRGPIIAGLA
RAGRADIEAAARAVELAEKPRIHTFIATSPIHMAKKLQLEPDAVIERAVEAVRLARSFVDDVEFSAEDATRSDRDFLVRI
FRAAVEAGATTINVPDTVGYTTPEEIRDLFAYLRGELPDHIILSAHCHDDLGMAVANSIAAAEGGARQIECTVNGIGERA
GNASLEEIVMAFHTRRDHYGFETGIRTREIYRTSRMVSRLSGMPVQPNKAVVGDNAFAHESGIHQDGVIKARETYEIMNA
ELVGREAAVLVMGKHSGRAAFRKALTDLGYAVDEERLKQLFARFKDMADRKGQIYADDLRALVESRSDVPQTFTLEGFQI
TSGMNMTPVAFVRLQTPDGPVDATAHGDGPVEAAFQAINKITGITPTLESYRIQAVTGGGDALGEVSIGARYGETTLHGT
GVATDVVEASARAWIRIVNQVVAGMGKSRAVSQTTV
>Mature_515_residues
TQPQAQAQRIRIFDTTLRDGEQSPGVALNHTQKLEIAHQLARLGVDVIEAGFPIASPGDLEGVSRIAREVRGPIIAGLAR
AGRADIEAAARAVELAEKPRIHTFIATSPIHMAKKLQLEPDAVIERAVEAVRLARSFVDDVEFSAEDATRSDRDFLVRIF
RAAVEAGATTINVPDTVGYTTPEEIRDLFAYLRGELPDHIILSAHCHDDLGMAVANSIAAAEGGARQIECTVNGIGERAG
NASLEEIVMAFHTRRDHYGFETGIRTREIYRTSRMVSRLSGMPVQPNKAVVGDNAFAHESGIHQDGVIKARETYEIMNAE
LVGREAAVLVMGKHSGRAAFRKALTDLGYAVDEERLKQLFARFKDMADRKGQIYADDLRALVESRSDVPQTFTLEGFQIT
SGMNMTPVAFVRLQTPDGPVDATAHGDGPVEAAFQAINKITGITPTLESYRIQAVTGGGDALGEVSIGARYGETTLHGTG
VATDVVEASARAWIRIVNQVVAGMGKSRAVSQTTV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=498, Percent_Identity=48.5943775100402, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320071, Length=387, Percent_Identity=27.1317829457364, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=383, Percent_Identity=26.1096605744125, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=542, Percent_Identity=24.7232472324723, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=25.3676470588235, Blast_Score=133, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24582381, Length=292, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55741; Mature: 55610

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPQAQAQRIRIFDTTLRDGEQSPGVALNHTQKLEIAHQLARLGVDVIEAGFPIASPGD
CCCCHHHHHHEEEEEHHHHCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
LEGVSRIAREVRGPIIAGLARAGRADIEAAARAVELAEKPRIHTFIATSPIHMAKKLQLE
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCC
PDAVIERAVEAVRLARSFVDDVEFSAEDATRSDRDFLVRIFRAAVEAGATTINVPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPEEIRDLFAYLRGELPDHIILSAHCHDDLGMAVANSIAAAEGGARQIECTVNGIGERA
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
GNASLEEIVMAFHTRRDHYGFETGIRTREIYRTSRMVSRLSGMPVQPNKAVVGDNAFAHE
CCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHC
SGIHQDGVIKARETYEIMNAELVGREAAVLVMGKHSGRAAFRKALTDLGYAVDEERLKQL
CCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
FARFKDMADRKGQIYADDLRALVESRSDVPQTFTLEGFQITSGMNMTPVAFVRLQTPDGP
HHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCCCC
VDATAHGDGPVEAAFQAINKITGITPTLESYRIQAVTGGGDALGEVSIGARYGETTLHGT
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEECCC
GVATDVVEASARAWIRIVNQVVAGMGKSRAVSQTTV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TQPQAQAQRIRIFDTTLRDGEQSPGVALNHTQKLEIAHQLARLGVDVIEAGFPIASPGD
CCCHHHHHHEEEEEHHHHCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHCCCCCCCCCC
LEGVSRIAREVRGPIIAGLARAGRADIEAAARAVELAEKPRIHTFIATSPIHMAKKLQLE
HHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCC
PDAVIERAVEAVRLARSFVDDVEFSAEDATRSDRDFLVRIFRAAVEAGATTINVPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCC
TTPEEIRDLFAYLRGELPDHIILSAHCHDDLGMAVANSIAAAEGGARQIECTVNGIGERA
CCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
GNASLEEIVMAFHTRRDHYGFETGIRTREIYRTSRMVSRLSGMPVQPNKAVVGDNAFAHE
CCCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHC
SGIHQDGVIKARETYEIMNAELVGREAAVLVMGKHSGRAAFRKALTDLGYAVDEERLKQL
CCCCCCCCEEHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHH
FARFKDMADRKGQIYADDLRALVESRSDVPQTFTLEGFQITSGMNMTPVAFVRLQTPDGP
HHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCCCC
VDATAHGDGPVEAAFQAINKITGITPTLESYRIQAVTGGGDALGEVSIGARYGETTLHGT
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEECCC
GVATDVVEASARAWIRIVNQVVAGMGKSRAVSQTTV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]