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The map label for this gene is yneF [C]

Identifier: 94984488

GI number: 94984488

Start: 384666

End: 386357

Strand: Reverse

Name: yneF [C]

Synonym: Dgeo_0380

Alternate gene names: 94984488

Gene position: 386357-384666 (Counterclockwise)

Preceding gene: 94984491

Following gene: 94984483

Centisome position: 15.66

GC content: 68.68

Gene sequence:

>1692_bases
ATGAAGCCTGCCCGCCGTCCGCAGCCGGTCCCTCTCCCGCCTGACGCTCCGCTCCAACTCCGCGAGGCGCTGAATGCCGC
GCGGATCGCAACTGCCTCTGCCTATCTGGCGCTGGCCCGCCACTACCGCAACCACTCACTCACGGTCGCGTTTGCGCTGG
CCCAGGAGGCGCTCGAAGCTGCCCTGAGCGCTGAGGCGCCCGCCGCCACCGTGGAGGTGCTGGCAGGCCTCGCGTTTATC
GAGGTGGCGCAGGGGCGGCAGGAGGCGGCCTTTGAACACCTCGCGCTCGCGCTCGATCTGGTGCACCAGTATGACCTCCG
GCATCTGGAAGCCCAGGTGCGGAACAACCGGGCCGTCGCGCGGCTGTCGGTCGGGGATACGGTCGGTGCACGGCGTGACC
TGCTGGACGCGCAGCTGCTGGCCCAAGCCGCGGGCGATCTGGTCGACGTGGCGCATGCCCACGTCAACCTGGCCTTTTTA
GAGAATGTGACCGGCCAGCCTGAGGAGGCGCTCCACCAGCTCAACCTGCTTGAAGAATTGCTCCCCAGCCTGCCCGACGA
GGCGACCCGTCAGTCGCTGGCCCTCTATCTCCATGAAAACCGCGCTCACAGCTATCTCAACCTGGTGCAGCAGGCCCGTG
AGCGCGGGCGCTCGGCAGCCGAGGCGGAAGCGCGCGCCCAGGCCCATGCCGAGATCCAAGCCACCCGCGAGGGCCTTGCG
CAGCAGCCCGCCGGTCTGATTGCCCTCACCACCGAAGCCCACGCCGCCCGGCTTGCGCTGCTCGAGGGCGATCTGGAACA
GGCCGAGCAGCATGCGCGGGCAGCACTTGACCACCACAACCAGCTGGGTCAGCAGATCTATCTGGAAGCCCACCTGGTCA
TGGCCGAGGTCAGCGCGGCGCAGGGCCAGCCGGAGCAGGCGCATGCGCACTACCGCGCGGCACTGGATTCGGCCCGCTAC
CAAAACCGCCACCGCGAGACGCAGGCTGTCCTGCGGGCCATCGCACGGCTGTACGAGCAGCAGGGCAACCTGCCAGCAGC
CCTCAACACCTACCGCGACGCCCTCGAGCGCGCGCAGCTCGCCCTCGACCGGCTGGCGCACATCGAGCAGCGCCATGCTG
ACCTGACCCGTGAGCTACATCAGGCGCGCGCCGAAGCACGAGGCTGGCAAGAAAGCGTGCGCCGCGCCGAAGCGCAGGCC
CGTCAGGACGCCTTGACCGGCCTGCTCAACCGCCGGGGGCTGCAAGACAGCCTGGCGCAGCTCACGCCGGACGCTGCCCC
GCTGCTGCTGGCCCTCTTTGACATCGACGACTTCAAGAGTGTCAACGACCACCACTCACACGCAACCGGCGACGCAGCCC
TCCAGGGGGTCGCGAACCGTCTGAGTGCCCTCGCCCCGGAAGGCAGCCTGCTGGCCCGCTACGGTGGTGAGGAATTCCTG
CTGGTGCTGCCCGGAGCCAACCCCGCAGAGGCCCCAGCGCTGATTGAGCGGCTGCGGCAAGGCATTGAGGTCCATAACTG
GTCTCCTCTGCTCCCCGGCATGTCCCTCACCGTCAGTGCGGGCTACACCCTCACGGAAGGGCTCGACGACGCCAGTCTCC
ACGCCGCGTTCCAGCAGGCCGACGACCACCTCTACCAGGCCAAACGGGCCGGACGGAACCGCATCTATCCGCCTCCCAAT
GAGCCGAGCTGA

Upstream 100 bases:

>100_bases
CTCCACCAAAAGACGCAGGGCGACGGTTGGGGGGGAAATACCCCTATCAAAAGGCCTGTAGGTGACGTTCTGGAAAGAGG
CGGTTCCAGAGAATGCCTCA

Downstream 100 bases:

>100_bases
CCTGCGCACCGGGGCCGCGGTGTTCTCTTCCCCCCGCGGCCCCGGTCCACTTGTCTCTGCTGTCTCCGCTGTCTCAGCGA
CTCAAAATCTCATCCACTCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MKPARRPQPVPLPPDAPLQLREALNAARIATASAYLALARHYRNHSLTVAFALAQEALEAALSAEAPAATVEVLAGLAFI
EVAQGRQEAAFEHLALALDLVHQYDLRHLEAQVRNNRAVARLSVGDTVGARRDLLDAQLLAQAAGDLVDVAHAHVNLAFL
ENVTGQPEEALHQLNLLEELLPSLPDEATRQSLALYLHENRAHSYLNLVQQARERGRSAAEAEARAQAHAEIQATREGLA
QQPAGLIALTTEAHAARLALLEGDLEQAEQHARAALDHHNQLGQQIYLEAHLVMAEVSAAQGQPEQAHAHYRAALDSARY
QNRHRETQAVLRAIARLYEQQGNLPAALNTYRDALERAQLALDRLAHIEQRHADLTRELHQARAEARGWQESVRRAEAQA
RQDALTGLLNRRGLQDSLAQLTPDAAPLLLALFDIDDFKSVNDHHSHATGDAALQGVANRLSALAPEGSLLARYGGEEFL
LVLPGANPAEAPALIERLRQGIEVHNWSPLLPGMSLTVSAGYTLTEGLDDASLHAAFQQADDHLYQAKRAGRNRIYPPPN
EPS

Sequences:

>Translated_563_residues
MKPARRPQPVPLPPDAPLQLREALNAARIATASAYLALARHYRNHSLTVAFALAQEALEAALSAEAPAATVEVLAGLAFI
EVAQGRQEAAFEHLALALDLVHQYDLRHLEAQVRNNRAVARLSVGDTVGARRDLLDAQLLAQAAGDLVDVAHAHVNLAFL
ENVTGQPEEALHQLNLLEELLPSLPDEATRQSLALYLHENRAHSYLNLVQQARERGRSAAEAEARAQAHAEIQATREGLA
QQPAGLIALTTEAHAARLALLEGDLEQAEQHARAALDHHNQLGQQIYLEAHLVMAEVSAAQGQPEQAHAHYRAALDSARY
QNRHRETQAVLRAIARLYEQQGNLPAALNTYRDALERAQLALDRLAHIEQRHADLTRELHQARAEARGWQESVRRAEAQA
RQDALTGLLNRRGLQDSLAQLTPDAAPLLLALFDIDDFKSVNDHHSHATGDAALQGVANRLSALAPEGSLLARYGGEEFL
LVLPGANPAEAPALIERLRQGIEVHNWSPLLPGMSLTVSAGYTLTEGLDDASLHAAFQQADDHLYQAKRAGRNRIYPPPN
EPS
>Mature_563_residues
MKPARRPQPVPLPPDAPLQLREALNAARIATASAYLALARHYRNHSLTVAFALAQEALEAALSAEAPAATVEVLAGLAFI
EVAQGRQEAAFEHLALALDLVHQYDLRHLEAQVRNNRAVARLSVGDTVGARRDLLDAQLLAQAAGDLVDVAHAHVNLAFL
ENVTGQPEEALHQLNLLEELLPSLPDEATRQSLALYLHENRAHSYLNLVQQARERGRSAAEAEARAQAHAEIQATREGLA
QQPAGLIALTTEAHAARLALLEGDLEQAEQHARAALDHHNQLGQQIYLEAHLVMAEVSAAQGQPEQAHAHYRAALDSARY
QNRHRETQAVLRAIARLYEQQGNLPAALNTYRDALERAQLALDRLAHIEQRHADLTRELHQARAEARGWQESVRRAEAQA
RQDALTGLLNRRGLQDSLAQLTPDAAPLLLALFDIDDFKSVNDHHSHATGDAALQGVANRLSALAPEGSLLARYGGEEFL
LVLPGANPAEAPALIERLRQGIEVHNWSPLLPGMSLTVSAGYTLTEGLDDASLHAAFQQADDHLYQAKRAGRNRIYPPPN
EPS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=33.5260115606936, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=39.2638036809816, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=34.5911949685535, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=34.8101265822785, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081977, Length=217, Percent_Identity=34.1013824884793, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=34.8837209302326, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=31.9767441860465, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.635838150289, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=33.5195530726257, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=181, Percent_Identity=27.6243093922652, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=195, Percent_Identity=31.2820512820513, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 61393; Mature: 61393

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPARRPQPVPLPPDAPLQLREALNAARIATASAYLALARHYRNHSLTVAFALAQEALEA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALSAEAPAATVEVLAGLAFIEVAQGRQEAAFEHLALALDLVHQYDLRHLEAQVRNNRAVA
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLSVGDTVGARRDLLDAQLLAQAAGDLVDVAHAHVNLAFLENVTGQPEEALHQLNLLEEL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LPSLPDEATRQSLALYLHENRAHSYLNLVQQARERGRSAAEAEARAQAHAEIQATREGLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QQPAGLIALTTEAHAARLALLEGDLEQAEQHARAALDHHNQLGQQIYLEAHLVMAEVSAA
HCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGQPEQAHAHYRAALDSARYQNRHRETQAVLRAIARLYEQQGNLPAALNTYRDALERAQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ALDRLAHIEQRHADLTRELHQARAEARGWQESVRRAEAQARQDALTGLLNRRGLQDSLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LTPDAAPLLLALFDIDDFKSVNDHHSHATGDAALQGVANRLSALAPEGSLLARYGGEEFL
HCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEE
LVLPGANPAEAPALIERLRQGIEVHNWSPLLPGMSLTVSAGYTLTEGLDDASLHAAFQQA
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHH
DDHLYQAKRAGRNRIYPPPNEPS
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPARRPQPVPLPPDAPLQLREALNAARIATASAYLALARHYRNHSLTVAFALAQEALEA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ALSAEAPAATVEVLAGLAFIEVAQGRQEAAFEHLALALDLVHQYDLRHLEAQVRNNRAVA
HHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RLSVGDTVGARRDLLDAQLLAQAAGDLVDVAHAHVNLAFLENVTGQPEEALHQLNLLEEL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LPSLPDEATRQSLALYLHENRAHSYLNLVQQARERGRSAAEAEARAQAHAEIQATREGLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
QQPAGLIALTTEAHAARLALLEGDLEQAEQHARAALDHHNQLGQQIYLEAHLVMAEVSAA
HCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGQPEQAHAHYRAALDSARYQNRHRETQAVLRAIARLYEQQGNLPAALNTYRDALERAQL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
ALDRLAHIEQRHADLTRELHQARAEARGWQESVRRAEAQARQDALTGLLNRRGLQDSLAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LTPDAAPLLLALFDIDDFKSVNDHHSHATGDAALQGVANRLSALAPEGSLLARYGGEEFL
HCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEE
LVLPGANPAEAPALIERLRQGIEVHNWSPLLPGMSLTVSAGYTLTEGLDDASLHAAFQQA
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHCCCCHHHHHHHHHHH
DDHLYQAKRAGRNRIYPPPNEPS
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA