Click here to switch to the map view.

The map label for this gene is mcl2 [H]

Identifier: 94984467

GI number: 94984467

Start: 364565

End: 365398

Strand: Reverse

Name: mcl2 [H]

Synonym: Dgeo_0359

Alternate gene names: 94984467

Gene position: 365398-364565 (Counterclockwise)

Preceding gene: 94984468

Following gene: 94984466

Centisome position: 14.81

GC content: 71.7

Gene sequence:

>834_bases
GTGAGCGTTAAACCCTGGCGTTCGGTGCTGTACGTGCCGGGCGACAAGCCGCGGGCCATCGAAAAGGCGCGGACGCTGCG
GGCCGACGCGGTGATCCTCGACTTGGAGGATGCCGTGGCCCCCGAACACAAGGACGCAGCGCGTGGGAACGTCAAGGCAG
CGCTGGCGTGGCGCTGGCCCGGCCCGCTGCTGGTGCGGGTAAACGGCCTAGGCACCCCCTGGGAACACGCCGACCGCGAG
ATGGCCCTGCTGGCCGGCGTGGACGGCCTGGTGCTGCCCAAGGTGGAGGACGCCGCCACCGTCCACGCACTGCACCTGCG
CGTGCCCCTCTGGCCGATGATCGAGACGCCGCTGGGCGTGCTGCGCGCCCCTGAGATCGCGGCCGTGCCGGGCGTGGCGG
GCCTGATTGTGGGAGCCAACGATCTCGCCCGGGTCCTGCGAACCCGTCCCCACCCCGAACGGCTGCCGCTGCTGCACGCC
CTCTCCGCAGTGGTGCTGGCCGCGCGCGCCCACGGCAAGGTGCCGCTGGACGCCGTGTTCAACGACGTGCGCGACCCGCA
GGGGTTCACCCGCGAGTGCGAACAGGGCCGCACCCTCGGCTTTGCCGGGAAGACCGTGATCCACCCCGACCAGGTGGAAC
TTGCCAACGGGGTCTTCGGCGTGACCGACGCGGAGGCCGACGGGGCCCGCGCCCTGCTCGCCGTCTGGGAGCAGGCCCGA
GCGGAAGGCAAAAGCGTGGCGACGTACCGGGGCACCCTGGTCGAGCAGATGCACGCCGACGAGGCGCGGGAAACGCTGGA
ACTGTGGGAGAAGGCAAGGTCCGGGCCGTACTGA

Upstream 100 bases:

>100_bases
CACCGTAACCTGAGCTCATGAAGCGTTCCCTGCCCTTCCTCCTCGCCCTCCTCCCCTTCCTGTTCGCTTCCAGTGCGGCG
CAGCGGCGGAGACAGCGGGC

Downstream 100 bases:

>100_bases
AGCTTCCTTAAAACCGGGCAAGGCGTTATCCCCACCAGGAACCACGCCCTCTTGCTTCATTCCGATAAAAACCTTTACTC
TAATTAAGCCATGCCGCTGA

Product: Citryl-CoA lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSVKPWRSVLYVPGDKPRAIEKARTLRADAVILDLEDAVAPEHKDAARGNVKAALAWRWPGPLLVRVNGLGTPWEHADRE
MALLAGVDGLVLPKVEDAATVHALHLRVPLWPMIETPLGVLRAPEIAAVPGVAGLIVGANDLARVLRTRPHPERLPLLHA
LSAVVLAARAHGKVPLDAVFNDVRDPQGFTRECEQGRTLGFAGKTVIHPDQVELANGVFGVTDAEADGARALLAVWEQAR
AEGKSVATYRGTLVEQMHADEARETLELWEKARSGPY

Sequences:

>Translated_277_residues
MSVKPWRSVLYVPGDKPRAIEKARTLRADAVILDLEDAVAPEHKDAARGNVKAALAWRWPGPLLVRVNGLGTPWEHADRE
MALLAGVDGLVLPKVEDAATVHALHLRVPLWPMIETPLGVLRAPEIAAVPGVAGLIVGANDLARVLRTRPHPERLPLLHA
LSAVVLAARAHGKVPLDAVFNDVRDPQGFTRECEQGRTLGFAGKTVIHPDQVELANGVFGVTDAEADGARALLAVWEQAR
AEGKSVATYRGTLVEQMHADEARETLELWEKARSGPY
>Mature_276_residues
SVKPWRSVLYVPGDKPRAIEKARTLRADAVILDLEDAVAPEHKDAARGNVKAALAWRWPGPLLVRVNGLGTPWEHADREM
ALLAGVDGLVLPKVEDAATVHALHLRVPLWPMIETPLGVLRAPEIAAVPGVAGLIVGANDLARVLRTRPHPERLPLLHAL
SAVVLAARAHGKVPLDAVFNDVRDPQGFTRECEQGRTLGFAGKTVIHPDQVELANGVFGVTDAEADGARALLAVWEQARA
EGKSVATYRGTLVEQMHADEARETLELWEKARSGPY

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=290, Percent_Identity=30.3448275862069, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI87081764, Length=285, Percent_Identity=35.0877192982456, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557458, Length=306, Percent_Identity=25.1633986928105, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 29990; Mature: 29859

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKPWRSVLYVPGDKPRAIEKARTLRADAVILDLEDAVAPEHKDAARGNVKAALAWRWP
CCCCCCCCEEECCCCCCHHHHHHHHHHHCEEEEECHHHCCCCCCHHCCCCEEEEEEEECC
GPLLVRVNGLGTPWEHADREMALLAGVDGLVLPKVEDAATVHALHLRVPLWPMIETPLGV
CCEEEEECCCCCCHHHCCCCEEEHCCCCCEECCCCCCCCEEEEEEEECCCCHHHHCCHHH
LRAPEIAAVPGVAGLIVGANDLARVLRTRPHPERLPLLHALSAVVLAARAHGKVPLDAVF
HCCCCHHCCCCHHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
NDVRDPQGFTRECEQGRTLGFAGKTVIHPDQVELANGVFGVTDAEADGARALLAVWEQAR
HHHCCCCCHHHHHHCCCEEEECCCEEECCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHH
AEGKSVATYRGTLVEQMHADEARETLELWEKARSGPY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SVKPWRSVLYVPGDKPRAIEKARTLRADAVILDLEDAVAPEHKDAARGNVKAALAWRWP
CCCCCCCEEECCCCCCHHHHHHHHHHHCEEEEECHHHCCCCCCHHCCCCEEEEEEEECC
GPLLVRVNGLGTPWEHADREMALLAGVDGLVLPKVEDAATVHALHLRVPLWPMIETPLGV
CCEEEEECCCCCCHHHCCCCEEEHCCCCCEECCCCCCCCEEEEEEEECCCCHHHHCCHHH
LRAPEIAAVPGVAGLIVGANDLARVLRTRPHPERLPLLHALSAVVLAARAHGKVPLDAVF
HCCCCHHCCCCHHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
NDVRDPQGFTRECEQGRTLGFAGKTVIHPDQVELANGVFGVTDAEADGARALLAVWEQAR
HHHCCCCCHHHHHHCCCEEEECCCEEECCCHHHHHCCEEECCCCCCCCHHHHHHHHHHHH
AEGKSVATYRGTLVEQMHADEARETLELWEKARSGPY
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA