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The map label for this gene is fabI [H]

Identifier: 94984368

GI number: 94984368

Start: 250238

End: 251023

Strand: Reverse

Name: fabI [H]

Synonym: Dgeo_0260

Alternate gene names: 94984368

Gene position: 251023-250238 (Counterclockwise)

Preceding gene: 94984373

Following gene: 94984367

Centisome position: 10.17

GC content: 67.18

Gene sequence:

>786_bases
ATGAGTGTGACCGTGGACCTCTCCGACAAGACTGCCCTGGTGATGGGCGTCGCCAACGCTCGCAGCCTAGGCTGGGCCAT
CGCGGAGCAGCTGCTCGCGGCGGGGTGCCGCGTGGGCTTTTCCTATCAGGGCGAGCGCCTCAGGCCCGAACTGGAAAAGT
TGACCGCCGGGCGCTCCGGCACCTGGATCCAACAGGCGGACGCCACCCGTGAAGACGACCTGACCGCGCTCTTTGCCCGC
GTTCGCGACGAATTCGGTCATCTGGACTATCTCGTCCACTCCATCGCCTTTGCGCCGCGCAGCGCGATGGAAGGCCGTTT
TCTCGACACGACCCCGGAAGACTGGAACACGGCCCTGAATGTCAGCGCCTATACCCTGGTCAGCACTGCCCGGCACGCCG
AACCGCTCCTCCGCGAGGGCGGCGCCGTGGTGAGTCTCACCTACCACGCCTCACAACAGGTCGTCCCCAAGTACAACGTG
ATGGGCGTGGCGAAGGCGGCGCTGGAGGCCGCCACCCGCTACCTCGCCTCCGAGCTGGGCGCGCGGGGCGTGCGGGTGAA
TACCATCAGCGCCGGACCGATGCGCACCATCGCCGCCCGCTCGATCCCCGGCTTCGGCACGATGTACGAGAAGTCGGCAC
AAAACGCGCCCTTGGGGCGCAACGCCACCCCCGAGGAGGTCGGCAAGCTGGCCCTCTTCCTGCTCTCGGACCTGGGCAGC
GGCGTCACCGGGCAGACGGTGTACGTGGACGCCGGGGCAAGCATCATGAGTATGAAGGTGGAGTGA

Upstream 100 bases:

>100_bases
CGAGATTCTGACGCGTCAATCCCCCGGCGGGGGCAGCGAAAGATGAGGCTTTTGCACCCGCTTCAGCAACGGGCCGCCCC
GCACTGCTACAGTCGCCCGC

Downstream 100 bases:

>100_bases
AGGCAAAGGCCCGTTATGATCCCCCCATGTCCCCCACCACGGCCGCGCTCGTGCTGCTCACCGCCCAGCGTCACCATTTG
GAGGCGTTGCCGGACGAGCG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSVTVDLSDKTALVMGVANARSLGWAIAEQLLAAGCRVGFSYQGERLRPELEKLTAGRSGTWIQQADATREDDLTALFAR
VRDEFGHLDYLVHSIAFAPRSAMEGRFLDTTPEDWNTALNVSAYTLVSTARHAEPLLREGGAVVSLTYHASQQVVPKYNV
MGVAKAALEAATRYLASELGARGVRVNTISAGPMRTIAARSIPGFGTMYEKSAQNAPLGRNATPEEVGKLALFLLSDLGS
GVTGQTVYVDAGASIMSMKVE

Sequences:

>Translated_261_residues
MSVTVDLSDKTALVMGVANARSLGWAIAEQLLAAGCRVGFSYQGERLRPELEKLTAGRSGTWIQQADATREDDLTALFAR
VRDEFGHLDYLVHSIAFAPRSAMEGRFLDTTPEDWNTALNVSAYTLVSTARHAEPLLREGGAVVSLTYHASQQVVPKYNV
MGVAKAALEAATRYLASELGARGVRVNTISAGPMRTIAARSIPGFGTMYEKSAQNAPLGRNATPEEVGKLALFLLSDLGS
GVTGQTVYVDAGASIMSMKVE
>Mature_260_residues
SVTVDLSDKTALVMGVANARSLGWAIAEQLLAAGCRVGFSYQGERLRPELEKLTAGRSGTWIQQADATREDDLTALFARV
RDEFGHLDYLVHSIAFAPRSAMEGRFLDTTPEDWNTALNVSAYTLVSTARHAEPLLREGGAVVSLTYHASQQVVPKYNVM
GVAKAALEAATRYLASELGARGVRVNTISAGPMRTIAARSIPGFGTMYEKSAQNAPLGRNATPEEVGKLALFLLSDLGSG
VTGQTVYVDAGASIMSMKVE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=271, Percent_Identity=27.3062730627306, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=29.7619047619048, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4503301, Length=207, Percent_Identity=28.9855072463768, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI126723750, Length=252, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=28.515625, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=38.1889763779528, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=29.3893129770992, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786812, Length=268, Percent_Identity=27.9850746268657, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=26.3779527559055, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=25.3061224489796, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=271, Percent_Identity=28.7822878228782, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17560676, Length=252, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=26.5682656826568, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=271, Percent_Identity=27.6752767527675, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=25.5639097744361, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=256, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=178, Percent_Identity=30.3370786516854, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=269, Percent_Identity=27.1375464684015, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994604, Length=178, Percent_Identity=29.7752808988764, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=265, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=250, Percent_Identity=25.6, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=273, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=271, Percent_Identity=25.4612546125461, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=26.5873015873016, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=28.4584980237154, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24644337, Length=121, Percent_Identity=33.8842975206612, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=25.968992248062, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27898; Mature: 27767

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTVDLSDKTALVMGVANARSLGWAIAEQLLAAGCRVGFSYQGERLRPELEKLTAGRSG
CEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC
TWIQQADATREDDLTALFARVRDEFGHLDYLVHSIAFAPRSAMEGRFLDTTPEDWNTALN
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEC
VSAYTLVSTARHAEPLLREGGAVVSLTYHASQQVVPKYNVMGVAKAALEAATRYLASELG
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ARGVRVNTISAGPMRTIAARSIPGFGTMYEKSAQNAPLGRNATPEEVGKLALFLLSDLGS
CCCEEEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GVTGQTVYVDAGASIMSMKVE
CCCCCEEEEECCCEEEEEECC
>Mature Secondary Structure 
SVTVDLSDKTALVMGVANARSLGWAIAEQLLAAGCRVGFSYQGERLRPELEKLTAGRSG
EEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCC
TWIQQADATREDDLTALFARVRDEFGHLDYLVHSIAFAPRSAMEGRFLDTTPEDWNTALN
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEC
VSAYTLVSTARHAEPLLREGGAVVSLTYHASQQVVPKYNVMGVAKAALEAATRYLASELG
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
ARGVRVNTISAGPMRTIAARSIPGFGTMYEKSAQNAPLGRNATPEEVGKLALFLLSDLGS
CCCEEEEEECCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GVTGQTVYVDAGASIMSMKVE
CCCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]