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The map label for this gene is pilT [H]

Identifier: 94984364

GI number: 94984364

Start: 246882

End: 247961

Strand: Reverse

Name: pilT [H]

Synonym: Dgeo_0256

Alternate gene names: 94984364

Gene position: 247961-246882 (Counterclockwise)

Preceding gene: 94984365

Following gene: 94984362

Centisome position: 10.05

GC content: 64.17

Gene sequence:

>1080_bases
ATGAGCGTCTTGCAGGCCTTGCTGGGAGTCATGGTGAAGTCGGGAGCCAGCGATATTCACCTGCGGTCTGGGAGTGCACC
CGCCGCACGGATCAATGGAAATATCGTCCGTTTTGGGGAAGACCGGCTCTCGCCTGACCACGTGGCTGGGTTTGCCCGCG
AGATGATGAGCCGCCCGGGTCTCTGGGAAGAGTTCATCACGCGCCGCGACGCCGACTTCGCGTATGGGGTTCCAGGTGTC
GCTCGGTTCCGCGTGAACGCCTACTACCAGCGCGGCACAGTCGGCCTGATCATGCGCGTGATCGAAGAAAAACCCATTCC
CAGCTTTGCGGAACTGGGCCTGCCCGTTGCGACCTTCGAGGCGCTGGCCGCCCAGGAACGCGGCTTGGTGCTGGTGACCG
GCCCCACCGGCTCGGGCAAGACGACCACCCTGGCTTCGATCCTCGACCACATCAACGCCACCCAGCCCGTCAACATCGTC
ACGCTGGAAGACCCCATCGAGATCCTGCACCGTGACAAGCAGGCCCTGGTGTCGCAGCGCGAACTGGGCACCGATACCCT
CAGCTTCGCGGCGGGCCTGCGCGCGGCCATGCGCCAGGATCCGGATGTGATCCTGATCGGCGAGATGCGCGACAAGGAGA
CGGTGGAGGCCGCCCTCTCTGCCGCGCAGACCGGACATCTGGTCTTCTCGACCCTCCACACCCTCGACGCCATCCGCACG
GTCAACCGCATCATCGATTTCTTCGCGCCACACGAACGCGACCAGATTCGCCTTGCCCTCTCTGAGAGCCTGGTCGGCGT
GGTCAGTCAGCGGCTGCTGCCGCGCCAGGGCGGGGGTCGGGTGCTGGGCATGGAAATCTTGCTCGGCACGCCCACCGTCC
GCGAGTGCATCAAGGATGCGAACCGCACCGAGGAAATCAAGCAAGCGCTTCAGGAAGGCAGCCTGCGCGGCATGCACACC
TTTGATCAGCATCTCGTCCAATTGGTGCAAGATCACCTGATCACTGAGGAAGACGCCCTGCAAGCCGCCACCAGCCCCCA
CGAACTGAAGCTCGCGCTGCTGAGCCGGCAGTTCGCGTAA

Upstream 100 bases:

>100_bases
ATCACCTCGGCCCTCACCATTCGGCCTCATCCCCGCTGGCTCCAATTGTGGATTAAGGAAGAATTAAAACTGTAAGACTC
CAGCCGCTAGAGTCGCAGGG

Downstream 100 bases:

>100_bases
GAGCGAGCACAGGCACCCGCGCACCTCCTTCCCTCCGCCGGTTAGAGCTTATGGCGATAGGGAAAAGCTCGTTCCGTCCG
GCGTCGAGCTGAGGGGTCCG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSVLQALLGVMVKSGASDIHLRSGSAPAARINGNIVRFGEDRLSPDHVAGFAREMMSRPGLWEEFITRRDADFAYGVPGV
ARFRVNAYYQRGTVGLIMRVIEEKPIPSFAELGLPVATFEALAAQERGLVLVTGPTGSGKTTTLASILDHINATQPVNIV
TLEDPIEILHRDKQALVSQRELGTDTLSFAAGLRAAMRQDPDVILIGEMRDKETVEAALSAAQTGHLVFSTLHTLDAIRT
VNRIIDFFAPHERDQIRLALSESLVGVVSQRLLPRQGGGRVLGMEILLGTPTVRECIKDANRTEEIKQALQEGSLRGMHT
FDQHLVQLVQDHLITEEDALQAATSPHELKLALLSRQFA

Sequences:

>Translated_359_residues
MSVLQALLGVMVKSGASDIHLRSGSAPAARINGNIVRFGEDRLSPDHVAGFAREMMSRPGLWEEFITRRDADFAYGVPGV
ARFRVNAYYQRGTVGLIMRVIEEKPIPSFAELGLPVATFEALAAQERGLVLVTGPTGSGKTTTLASILDHINATQPVNIV
TLEDPIEILHRDKQALVSQRELGTDTLSFAAGLRAAMRQDPDVILIGEMRDKETVEAALSAAQTGHLVFSTLHTLDAIRT
VNRIIDFFAPHERDQIRLALSESLVGVVSQRLLPRQGGGRVLGMEILLGTPTVRECIKDANRTEEIKQALQEGSLRGMHT
FDQHLVQLVQDHLITEEDALQAATSPHELKLALLSRQFA
>Mature_358_residues
SVLQALLGVMVKSGASDIHLRSGSAPAARINGNIVRFGEDRLSPDHVAGFAREMMSRPGLWEEFITRRDADFAYGVPGVA
RFRVNAYYQRGTVGLIMRVIEEKPIPSFAELGLPVATFEALAAQERGLVLVTGPTGSGKTTTLASILDHINATQPVNIVT
LEDPIEILHRDKQALVSQRELGTDTLSFAAGLRAAMRQDPDVILIGEMRDKETVEAALSAAQTGHLVFSTLHTLDAIRTV
NRIIDFFAPHERDQIRLALSESLVGVVSQRLLPRQGGGRVLGMEILLGTPTVRECIKDANRTEEIKQALQEGSLRGMHTF
DQHLVQLVQDHLITEEDALQAATSPHELKLALLSRQFA

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=323, Percent_Identity=40.2476780185758, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786296, Length=322, Percent_Identity=33.2298136645963, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789723, Length=292, Percent_Identity=33.9041095890411, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39258; Mature: 39126

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLQALLGVMVKSGASDIHLRSGSAPAARINGNIVRFGEDRLSPDHVAGFAREMMSRPG
CHHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHCCC
LWEEFITRRDADFAYGVPGVARFRVNAYYQRGTVGLIMRVIEEKPIPSFAELGLPVATFE
HHHHHHHHCCCCCCCCCCCHHHEEEHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCHHHHH
ALAAQERGLVLVTGPTGSGKTTTLASILDHINATQPVNIVTLEDPIEILHRDKQALVSQR
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHH
ELGTDTLSFAAGLRAAMRQDPDVILIGEMRDKETVEAALSAAQTGHLVFSTLHTLDAIRT
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VNRIIDFFAPHERDQIRLALSESLVGVVSQRLLPRQGGGRVLGMEILLGTPTVRECIKDA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHC
NRTEEIKQALQEGSLRGMHTFDQHLVQLVQDHLITEEDALQAATSPHELKLALLSRQFA
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SVLQALLGVMVKSGASDIHLRSGSAPAARINGNIVRFGEDRLSPDHVAGFAREMMSRPG
HHHHHHHHHHHHCCCCCEEEECCCCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHCCC
LWEEFITRRDADFAYGVPGVARFRVNAYYQRGTVGLIMRVIEEKPIPSFAELGLPVATFE
HHHHHHHHCCCCCCCCCCCHHHEEEHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCHHHHH
ALAAQERGLVLVTGPTGSGKTTTLASILDHINATQPVNIVTLEDPIEILHRDKQALVSQR
HHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCHHHHHHHH
ELGTDTLSFAAGLRAAMRQDPDVILIGEMRDKETVEAALSAAQTGHLVFSTLHTLDAIRT
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VNRIIDFFAPHERDQIRLALSESLVGVVSQRLLPRQGGGRVLGMEILLGTPTVRECIKDA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHHHHHHC
NRTEEIKQALQEGSLRGMHTFDQHLVQLVQDHLITEEDALQAATSPHELKLALLSRQFA
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]