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The map label for this gene is truA [H]

Identifier: 94984145

GI number: 94984145

Start: 34598

End: 35398

Strand: Reverse

Name: truA [H]

Synonym: Dgeo_0037

Alternate gene names: 94984145

Gene position: 35398-34598 (Counterclockwise)

Preceding gene: 94984148

Following gene: 94984139

Centisome position: 1.43

GC content: 69.91

Gene sequence:

>801_bases
ATGACCACTCCGCCCCGCTTTGTTCCTCCGCACGGTTTCCGGCGCTTGCGGCTGCTGCTTGCCTGGGACGGCACGGACTT
CGCGGGATGGCAGGCCCAGCGGGACGTACCGAGCGTGCAGGATACCCTGTATGCCGCACTGCTGCGCTTGACGGAGGAGG
CAGCTCGACCCGTCGCCGCGGGCCGTACCGACGCAGGTGTTCATGCCGAGGCGATGCCCGCCCACGTGGACGTGCCGCAG
AGCTTCCGTATTCCCGCCAAGAAGCTTGCCCGGGCGCTGAACGCCCACCTTCCCCCCAGCGTGGCAGTGCTGGAAGCTGA
GGAGGCCCCCACTGGCTTCCACGCCCGCTTTTCCTGCACCGAGCGGCGGTACACCTACCGTCTGCTGAACACCCCGCAGC
GCCATCCCCTCTGGAGGGGCCGCGCACTGCATGTCCCCGGCGCCCTCGACGTGCCTGCGATGAACGCAGCCGCCGCTTGT
TTGCTCGGCACCCACGACTTTGCCGCCTTTGCCACCCGCGAGGACCGCCAGACGGTGCGGGAACTGCGGGCACTCGCCGT
TCACCCCGGCCCGGTGGTTCCCGGCGGAAGGATTTGGGAGGTGCGGGTCGCGGGGGAGAGCTTCCTGCGCCAGATGGTGC
GCGGGCTGGTGGGCACGCTGCTGTTGGTGGGGCAGGGCAAACTGGGGCAGGAGGACGTGACGGCCGTCCTCGTCGGGCGC
GAACGGGCGCGGGCCGGGGCAAATGTGCCGGGCTATGGGCTGACGTTCACGGGGGCGAGCTACGGAGGGTTTGAGGCCTG
A

Upstream 100 bases:

>100_bases
CCGGAAGAGCACCGGATGAGAACGGGGGTCACAATCCTTAGCTTAACGGGGGAGTCCCGCGCGCTATGTGAGAAATTCCT
CCCCACGTTACCCTGCACTT

Downstream 100 bases:

>100_bases
GCTGCGCAAGGAGGCAACGCTTCTGCTCCGCGCCAATCACCCCCGCCGCGTCACCCCGTCCGTCGTCAGCAGTGGGCCGT
ACAGGTCGGGGCGGCGGTCC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTPPRFVPPHGFRRLRLLLAWDGTDFAGWQAQRDVPSVQDTLYAALLRLTEEAARPVAAGRTDAGVHAEAMPAHVDVPQ
SFRIPAKKLARALNAHLPPSVAVLEAEEAPTGFHARFSCTERRYTYRLLNTPQRHPLWRGRALHVPGALDVPAMNAAAAC
LLGTHDFAAFATREDRQTVRELRALAVHPGPVVPGGRIWEVRVAGESFLRQMVRGLVGTLLLVGQGKLGQEDVTAVLVGR
ERARAGANVPGYGLTFTGASYGGFEA

Sequences:

>Translated_266_residues
MTTPPRFVPPHGFRRLRLLLAWDGTDFAGWQAQRDVPSVQDTLYAALLRLTEEAARPVAAGRTDAGVHAEAMPAHVDVPQ
SFRIPAKKLARALNAHLPPSVAVLEAEEAPTGFHARFSCTERRYTYRLLNTPQRHPLWRGRALHVPGALDVPAMNAAAAC
LLGTHDFAAFATREDRQTVRELRALAVHPGPVVPGGRIWEVRVAGESFLRQMVRGLVGTLLLVGQGKLGQEDVTAVLVGR
ERARAGANVPGYGLTFTGASYGGFEA
>Mature_265_residues
TTPPRFVPPHGFRRLRLLLAWDGTDFAGWQAQRDVPSVQDTLYAALLRLTEEAARPVAAGRTDAGVHAEAMPAHVDVPQS
FRIPAKKLARALNAHLPPSVAVLEAEEAPTGFHARFSCTERRYTYRLLNTPQRHPLWRGRALHVPGALDVPAMNAAAACL
LGTHDFAAFATREDRQTVRELRALAVHPGPVVPGGRIWEVRVAGESFLRQMVRGLVGTLLLVGQGKLGQEDVTAVLVGRE
RARAGANVPGYGLTFTGASYGGFEA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=289, Percent_Identity=37.3702422145329, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI199559785, Length=261, Percent_Identity=26.0536398467433, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=33.2, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28829; Mature: 28698

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPPRFVPPHGFRRLRLLLAWDGTDFAGWQAQRDVPSVQDTLYAALLRLTEEAARPVAA
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GRTDAGVHAEAMPAHVDVPQSFRIPAKKLARALNAHLPPSVAVLEAEEAPTGFHARFSCT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEC
ERRYTYRLLNTPQRHPLWRGRALHVPGALDVPAMNAAAACLLGTHDFAAFATREDRQTVR
CHHEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHH
ELRALAVHPGPVVPGGRIWEVRVAGESFLRQMVRGLVGTLLLVGQGKLGQEDVTAVLVGR
HHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECC
ERARAGANVPGYGLTFTGASYGGFEA
HHHHCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TTPPRFVPPHGFRRLRLLLAWDGTDFAGWQAQRDVPSVQDTLYAALLRLTEEAARPVAA
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GRTDAGVHAEAMPAHVDVPQSFRIPAKKLARALNAHLPPSVAVLEAEEAPTGFHARFSCT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEC
ERRYTYRLLNTPQRHPLWRGRALHVPGALDVPAMNAAAACLLGTHDFAAFATREDRQTVR
CHHEEEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHH
ELRALAVHPGPVVPGGRIWEVRVAGESFLRQMVRGLVGTLLLVGQGKLGQEDVTAVLVGR
HHHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECC
ERARAGANVPGYGLTFTGASYGGFEA
HHHHCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]