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The map label for this gene is lacR2 [H]

Identifier: 94995132

GI number: 94995132

Start: 1689228

End: 1690001

Strand: Direct

Name: lacR2 [H]

Synonym: MGAS10750_Spy1736

Alternate gene names: 94995132

Gene position: 1689228-1690001 (Clockwise)

Preceding gene: 94995117

Following gene: 94995133

Centisome position: 87.2

GC content: 34.37

Gene sequence:

>774_bases
ATGAAAAAGAAAGAGCGTCATGAAAAAATTCTCGATATCTTAAAAGTTGACGGTTTTATAAAAGTAAAAGATATTATCGA
TGAAATGAACATCTCAGATATGACCGCTAGACGAGATCTCGATACATTGGCAGATAAAGGATTACTTATCAGGACCCATG
GTGGTGCTCAATATTTGGATTATTCTTCAGCAAAGGACGAAGGTCACGAGAAAACACACACTGAAAAGAAGGTCTTACAG
ACTACCGAAAAAAAACAAATTGCACAAAGAGCTAAAAACATTATTAAAGATGGCGAAACTGTCTTTATAGGTCCAGGGAC
AACATTAGAGCATCTAGCTGTCGAATTGAAAAATAGAAATATCAGGGTCATTACAAATAGCCTTCCTGTTTTTTTAATTT
TGCACCCGAGCACTACTATTGATTTGATTTTAATTGGTGGTGAATGTCGTGAAATTACTGGAGCTTTTGTTGGTGCTATT
GCCATTAATAATCTAGAATCATTAACTTTTTCAAAGGCTTTTGTTAGCGCAAATGCAGTTTACAATAATTCTATTGCGAC
GTACAGTGATCTCGAAGGAGAAGTTCAAAGGGTTGCATTGAATAATGCTGTTGAGAAAATCCTTCTAGTTGATAGTACCA
AGTTTGAAAGTTATGACTTCTATAATTTCTACCAACTAGATCAAGTGGACTTAATTGTGACTGATAAAAATGTGACAACT
GGTATCATAGAAAAGTACAAGAAATTCTCAAAAATCATAATCGCTGACAAGTAA

Upstream 100 bases:

>100_bases
TTTTTTCCAACATTTATGTTTGTTTTGTTTGATTTTTTTATTGATATTATGTTATGATACCTTTAATAGTTATACCCTAA
GACACTGATAGGAGATAAGA

Downstream 100 bases:

>100_bases
AGGACCTACTCATATTAAAAAGCACTTCAGGGTTTAGATATATCCCCTGTCAAGTAAACAGGGAGCATATCTGAAAGTAG
TGTTTGTATTATAAAGAAGT

Product: Lactose phosphotransferase system repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLADKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQ
TTEKKQIAQRAKNIIKDGETVFIGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESYDFYNFYQLDQVDLIVTDKNVTT
GIIEKYKKFSKIIIADK

Sequences:

>Translated_257_residues
MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLADKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQ
TTEKKQIAQRAKNIIKDGETVFIGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESYDFYNFYQLDQVDLIVTDKNVTT
GIIEKYKKFSKIIIADK
>Mature_257_residues
MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLADKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQ
TTEKKQIAQRAKNIIKDGETVFIGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESYDFYNFYQLDQVDLIVTDKNVTT
GIIEKYKKFSKIIIADK

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=246, Percent_Identity=29.6747967479675, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI226510968, Length=237, Percent_Identity=29.535864978903, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87082344, Length=238, Percent_Identity=29.8319327731092, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789519, Length=246, Percent_Identity=26.0162601626016, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789059, Length=242, Percent_Identity=30.5785123966942, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789170, Length=243, Percent_Identity=27.1604938271605, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790635, Length=251, Percent_Identity=25.8964143426295, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787540, Length=237, Percent_Identity=27.0042194092827, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790753, Length=251, Percent_Identity=25.0996015936255, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788069, Length=240, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787063, Length=236, Percent_Identity=27.5423728813559, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 28881; Mature: 28881

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLADKGLLIRTHGGAQYLD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEC
YSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVFIGPGTTLEHLAVELKNRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCC
IRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAIAINNLESLTFSKAFVSANAV
EEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCE
YNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESYDFYNFYQLDQVDLIVTDKNVTT
ECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCEEEEEECCCCHH
GIIEKYKKFSKIIIADK
HHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLADKGLLIRTHGGAQYLD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEC
YSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVFIGPGTTLEHLAVELKNRN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCC
IRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAIAINNLESLTFSKAFVSANAV
EEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCE
YNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESYDFYNFYQLDQVDLIVTDKNVTT
ECCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEECCEEEEEECCCCHH
GIIEKYKKFSKIIIADK
HHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120234; 1901863 [H]