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The map label for this gene is dnaI [H]

Identifier: 94995076

GI number: 94995076

Start: 1622498

End: 1623325

Strand: Direct

Name: dnaI [H]

Synonym: MGAS10750_Spy1680

Alternate gene names: 94995076

Gene position: 1622498-1623325 (Clockwise)

Preceding gene: 94995075

Following gene: 94995077

Centisome position: 83.76

GC content: 29.47

Gene sequence:

>828_bases
ATGAAAAGTTTAAAAGATTTTGTATTAAGAGAAAATGATATTGAAAGAAATGGACATATCTATTGTAAATCATGTGGTAA
AAGAGTCGATGGAGAATTACTTGACCTTGGATTTACAAAGTTTATTCCAAGAATTAAATGTGAATGTGAAATAAAAAGAG
ATAAGGAAAACGAAGAAAGAGAAAGATTAATGAAAATATCATCGCTAAAAAGAGATTGCTTCTCATCGCCACTCCAACAC
CAATATACTTTTGAGAAATACTTAAATGAAAAAGGTCAAGCCTATAGGGTTGCCTACAACTATGCTAAGAGCTTTGAACA
AATGAAAGAAGACAATGTTGGACTTTTATTTTATGGAGATGTTGGTAGTGGAAAGACTTATCTTGCTTGTTCAATTGCAA
ATGAATTAATTGAAAGAAAGCAAGTTAAAGTTAAGATTATGAATTTATCTCAGGTTATAAACCAAATACAAAAATCAGCA
TTTAAGCTAGATTCAAATGAAATTATAGATAACCTATCAAACATTCCGTTGTTAATTTTAGATGATCTTGGAATTGAAAG
GGATACATCTTACGCAAGAGAACAAGTATATAACATTATAAACTCAAGATACTTAAAGGGTAGGCCGACAATTTTTACTA
CAAACTTATCTTTGGAAATTATTCAAAATCCTAATATTGACCTTGAGTATCAGAGGATCTATTCAAGAATACTTGAAATG
ACAATACCAGTTAAAGTTACGGGAGAAGATTTTAGAAGAAAAATCCATCAAGAGAAGTTAAGAAAATACAAAGAGTTACT
TTTATATGGAGGTGGAATAGATGATTAA

Upstream 100 bases:

>100_bases
ATCACAAGGCAACAATAATTAACTGGTATCTTAATGATCAGGCGAAAAGCATTAATGACAAATCAACAAGAAAAATGAAT
TACGATATAGGAGAGAGTTT

Downstream 100 bases:

>100_bases
TGAAGAAGTAACTAGGTCAAGTCTTAATCTTGAAGTAAGACTTGCAAAAGCAACAAGTAAAGCAATTCTTGATGCCTTAA
AGAAAGTACACAAGCAAATA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKSLKDFVLRENDIERNGHIYCKSCGKRVDGELLDLGFTKFIPRIKCECEIKRDKENEERERLMKISSLKRDCFSSPLQH
QYTFEKYLNEKGQAYRVAYNYAKSFEQMKEDNVGLLFYGDVGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSA
FKLDSNEIIDNLSNIPLLILDDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSLEIIQNPNIDLEYQRIYSRILEM
TIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD

Sequences:

>Translated_275_residues
MKSLKDFVLRENDIERNGHIYCKSCGKRVDGELLDLGFTKFIPRIKCECEIKRDKENEERERLMKISSLKRDCFSSPLQH
QYTFEKYLNEKGQAYRVAYNYAKSFEQMKEDNVGLLFYGDVGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSA
FKLDSNEIIDNLSNIPLLILDDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSLEIIQNPNIDLEYQRIYSRILEM
TIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD
>Mature_275_residues
MKSLKDFVLRENDIERNGHIYCKSCGKRVDGELLDLGFTKFIPRIKCECEIKRDKENEERERLMKISSLKRDCFSSPLQH
QYTFEKYLNEKGQAYRVAYNYAKSFEQMKEDNVGLLFYGDVGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSA
FKLDSNEIIDNLSNIPLLILDDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSLEIIQNPNIDLEYQRIYSRILEM
TIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD

Specific function: Probably involved in DNA replication [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790823, Length=167, Percent_Identity=29.3413173652695, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787623, Length=180, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009928
- InterPro:   IPR002611 [H]

Pfam domain/function: PF07319 DnaI_N; PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 32211; Mature: 32211

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLKDFVLRENDIERNGHIYCKSCGKRVDGELLDLGFTKFIPRIKCECEIKRDKENEER
CCCHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHCCCCEEEEEECCCCCHHHH
ERLMKISSLKRDCFSSPLQHQYTFEKYLNEKGQAYRVAYNYAKSFEQMKEDNVGLLFYGD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEC
VGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSAFKLDSNEIIDNLSNIPLLIL
CCCCCEEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
DDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSLEIIQNPNIDLEYQRIYSRILEM
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHH
TIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD
HCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSLKDFVLRENDIERNGHIYCKSCGKRVDGELLDLGFTKFIPRIKCECEIKRDKENEER
CCCHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHCCHHHHCCCCEEEEEECCCCCHHHH
ERLMKISSLKRDCFSSPLQHQYTFEKYLNEKGQAYRVAYNYAKSFEQMKEDNVGLLFYGD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEC
VGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSAFKLDSNEIIDNLSNIPLLIL
CCCCCEEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEE
DDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSLEIIQNPNIDLEYQRIYSRILEM
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHH
TIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD
HCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027671; 8969504; 9387221; 9384377; 3027697; 1549481; 7773414 [H]