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The map label for this gene is topA [H]

Identifier: 94994430

GI number: 94994430

Start: 981090

End: 983219

Strand: Direct

Name: topA [H]

Synonym: MGAS10750_Spy1034

Alternate gene names: 94994430

Gene position: 981090-983219 (Clockwise)

Preceding gene: 94994429

Following gene: 94994434

Centisome position: 50.65

GC content: 37.93

Gene sequence:

>2130_bases
TTGGGAACAAAAACTACAACTAAGCCAAAAACGGGTACTAAAAAGTCCACCACTAAAAAGAAATCAACAGCGAAAAAAAA
TCTCGTTATTGTTGAATCACCTGCCAAAGCTAAGACTATTGAAAAATATCTTGGACGCAGTTATAAAGTTGTTGCTTCGG
TAGGACATATTCGTGACTTAAAAAAATCGTCTATGTCTATTGATTTTGATAATAATTATGAGCCACAATACATTAATATC
CGTGGTAAGGGACCCCTTATCAATTCTTTAAAAAAAGAAGCCAAAGCTGCTAAAAAAGTTTATTTAGCTAGTGACCCGGA
TCGTGAGGGAGAGGCTATTTCTTGGCATTTGTCACATATCTTAGGACTTGATCCTCAAGATAATAACCGAGTGGTTTTTA
ATGAAATTACGAAAGATGCAGTTAAACATGCCTTTGTGGAGCCTCGTCAGATTGATATGGACTTGGTAGATTCTCAACAA
GCACGTCGTGTTTTGGACCGTATTGTGGGATATTCCATTTCACCAATTCTTTGGAAAAAGGTCAAAAAAGGACTTTCAGC
AGGACGTGTCCAGTCGGTTGCTTTAAAACTCATCATTGACCGTGAAAATGACATCAAAGCATTTGTTCCAAAGGAATACT
GGTCTATTGATGGTCTTTTTAAAAAAGGAACTAAAAAGTTTCAAGCGACTTTTTACGGTATTAATGGCAAAAAAACAAAG
CTTGATAATAACAACGATGTCAAAGAGGTATTAGCAAAGCTAACAAATGAAGATTTTCTTGTCAGCAAGGTTGACAAGAA
AGAGCGACGTCGTAATGCTCCCTTACCTTACACAACATCATCTCTTCAACAAGATGCTGCTAATAAAATCAATTTCAGAA
CGCGTAAAACCATGATGGTAGCCCAACAACTGTATGAGGGGATTCATTTAGGAGAAAATGGAACTCAAGGTTTAATTACC
TATATGCGTACGGATTCTACTCGTATCAGTCCAGTAGCACAAAACGATGCTGCTCAATTTATTATCAATCGCTTTGGAGC
TAATTATTCAAAACATGGTAATCGTGTCAAAAATACCAGTGGTGCCCAAGATGCTCACGAGGCGATTCGTCCATCTAGTG
TTAATCATACGCCAGACTCTATTGCCAAATACCTTAACAAGGATCAGTTAAAACTTTATACGCTTATTTGGAATCGTTTT
GTGGCTAGCCAAATGACAGCAGCTGTTTTTGATACCGTAAAGGTGAACTTGGAACAAAATGGCGTGATTTTTGTTGCCAA
TGGTAGCCAAATGAAGTTTGATGGTTACATGGCTGTTTACAATGATTCCGATAAAAACAAAAAGTTACCTGAGATGGCAG
AAGGAGAAACTGTTAAAAAAATATCGACCTCTCCAGAACAGCACTTTACGCAGCCACCAGCTCGTTATTCTGAAGCAACT
CTGATTAAAACGCTAGAAGAAAATGGTGTTGGACGTCCATCAACTTACGCTCCAACCTTAGAAGTCATTCAACGCAGATA
CTATGTTAAATTGTCTGCTAAGCGCTTTGAGCCAACTGAGCTTGGGGAAATTGTCAACAAGCTTATTGTCGAATTTTTCC
CAGATATTGTTGATGTTGCCTTTACTGCCGAGATGGAAGGTAAGCTAGATCAAGTTGAAATCGGAGAAGAACAATGGCAA
CATGTTATAGATCAATTCTATCAACCCTTTGTCAAAGAATTGAATAAGGCTGAGTCTGAAATCGAAAAGATTCAAATCAA
AGATGAACCAGCTGGTTTCGACTGTGATGTTTGTGGTCACCCAATGGTCATTAAGTTAGGACGTTTTGGGAAATTTTACG
CGTGTAGCAACTTCCCAGAGTGCCGTAATACAAAAGCAATCACTAAAGAAATTGGTGTTACTTGTCCGGTTTGTCATAAA
GGACAAGTTATCGAGCGTAAAACCAAAAAAAATAGGATATTTTATGGTTGTGATCAGTATCCAGATTGTGAGTTTATCTC
GTGGGATTTACCTATTGGACGTGCTTGCCCTAAGTCAGGTGACTATCTTATTGAGAAAAAAGTTCGTGGTGGCAAACAAG
TAATGTGTAGCAATGAGACCTGTGATTACAAGGAAGAAAAAATTAAATAA

Upstream 100 bases:

>100_bases
TAAAGTTTCTTCCTATATAGCATTTTTTTATTGACAGTTTCTTAAAAATGGGATATTATGCTATAAGCTTTAAACAAACT
GTTATAGGAAGTGTGTCGAA

Downstream 100 bases:

>100_bases
AAAAAGAATTGGAACTCCTTGTCTTGTTCCAATTCTTTTTTTTATTTACAAAGGTATTTTTAACTACAAAAATCGCTAGT
TCGTTTTTAAAAGGCTTAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 709; Mature: 708

Protein sequence:

>709_residues
MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINI
RGKGPLINSLKKEAKAAKKVYLASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDGLFKKGTKKFQATFYGINGKKTK
LDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLIT
YMRTDSTRISPVAQNDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKLYTLIWNRF
VASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKKLPEMAEGETVKKISTSPEQHFTQPPARYSEAT
LIKTLEENGVGRPSTYAPTLEVIQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQ
HVIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNTKAITKEIGVTCPVCHK
GQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDYLIEKKVRGGKQVMCSNETCDYKEEKIK

Sequences:

>Translated_709_residues
MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINI
RGKGPLINSLKKEAKAAKKVYLASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDGLFKKGTKKFQATFYGINGKKTK
LDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLIT
YMRTDSTRISPVAQNDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKLYTLIWNRF
VASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKKLPEMAEGETVKKISTSPEQHFTQPPARYSEAT
LIKTLEENGVGRPSTYAPTLEVIQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQ
HVIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNTKAITKEIGVTCPVCHK
GQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDYLIEKKVRGGKQVMCSNETCDYKEEKIK
>Mature_708_residues
GTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIR
GKGPLINSLKKEAKAAKKVYLASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQA
RRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDGLFKKGTKKFQATFYGINGKKTKL
DNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITY
MRTDSTRISPVAQNDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKLYTLIWNRFV
ASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKKLPEMAEGETVKKISTSPEQHFTQPPARYSEATL
IKTLEENGVGRPSTYAPTLEVIQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQH
VIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNTKAITKEIGVTCPVCHKG
QVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDYLIEKKVRGGKQVMCSNETCDYKEEKIK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=617, Percent_Identity=26.742301458671, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4507635, Length=662, Percent_Identity=20.5438066465257, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1787529, Length=751, Percent_Identity=38.2157123834887, Blast_Score=484, Evalue=1e-137,
Organism=Escherichia coli, GI1788061, Length=539, Percent_Identity=23.9332096474954, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=39.0909090909091, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=559, Percent_Identity=24.3291592128801, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17555378, Length=508, Percent_Identity=24.4094488188976, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=514, Percent_Identity=23.3463035019455, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585251, Length=620, Percent_Identity=23.2258064516129, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640096, Length=628, Percent_Identity=21.1783439490446, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80267; Mature: 80136

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDL
CCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEHHHHHHHHH
KKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYLASDPDREGEAISWHLSHI
HHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEHHHH
LGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQARRVLDRIVGYSISPILWKK
HCCCCCCCCEEEHHHHHHHHHHHHHCCCHHCCHHHHCCHHHHHHHHHHHCCCCCHHHHHH
VKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDGLFKKGTKKFQATFYGINGKKTK
HHHCCCCCHHHHEEEEEEEECCCCCEEECCHHHHCHHHHHHHCCCEEEEEEEECCCCEEC
LDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLPYTTSSLQQDAANKINFRTRKTMMV
CCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
AQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQNDAAQFIINRFGANYSKHGNRVKNTS
HHHHHHCEECCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCC
GAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKLYTLIWNRFVASQMTAAVFDTVKVNLEQN
CCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
GVIFVANGSQMKFDGYMAVYNDSDKNKKLPEMAEGETVKKISTSPEQHFTQPPARYSEAT
CEEEEECCCEEEECCEEEEECCCCCCCCCCCHHCCCHHHHHCCCCHHHCCCCCCHHHHHH
LIKTLEENGVGRPSTYAPTLEVIQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVA
HHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTAEMEGKLDQVEIGEEQWQHVIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGH
HEEHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
PMVIKLGRFGKFYACSNFPECRNTKAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQY
CEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCC
PDCEFISWDLPIGRACPKSGDYLIEKKVRGGKQVMCSNETCDYKEEKIK
CCCCEEEECCCCCCCCCCCCCEEEEHHHCCCCEEEECCCCCCCHHHCCC
>Mature Secondary Structure 
GTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYLGRSYKVVASVGHIRDL
CCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEHHHHHHHHH
KKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYLASDPDREGEAISWHLSHI
HHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEHHHH
LGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQARRVLDRIVGYSISPILWKK
HCCCCCCCCEEEHHHHHHHHHHHHHCCCHHCCHHHHCCHHHHHHHHHHHCCCCCHHHHHH
VKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDGLFKKGTKKFQATFYGINGKKTK
HHHCCCCCHHHHEEEEEEEECCCCCEEECCHHHHCHHHHHHHCCCEEEEEEEECCCCEEC
LDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLPYTTSSLQQDAANKINFRTRKTMMV
CCCCCCHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
AQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQNDAAQFIINRFGANYSKHGNRVKNTS
HHHHHHCEECCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCC
GAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKLYTLIWNRFVASQMTAAVFDTVKVNLEQN
CCHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
GVIFVANGSQMKFDGYMAVYNDSDKNKKLPEMAEGETVKKISTSPEQHFTQPPARYSEAT
CEEEEECCCEEEECCEEEEECCCCCCCCCCCHHCCCHHHHHCCCCHHHCCCCCCHHHHHH
LIKTLEENGVGRPSTYAPTLEVIQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVA
HHHHHHHCCCCCCCCCCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTAEMEGKLDQVEIGEEQWQHVIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGH
HEEHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
PMVIKLGRFGKFYACSNFPECRNTKAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQY
CEEEEECCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEECCCCC
PDCEFISWDLPIGRACPKSGDYLIEKKVRGGKQVMCSNETCDYKEEKIK
CCCCEEEECCCCCCCCCCCCCEEEEHHHCCCCEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]