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The map label for this gene is lepA [H]

Identifier: 94994323

GI number: 94994323

Start: 882479

End: 884296

Strand: Direct

Name: lepA [H]

Synonym: MGAS10750_Spy0927

Alternate gene names: 94994323

Gene position: 882479-884296 (Clockwise)

Preceding gene: 94994322

Following gene: 94994324

Centisome position: 45.56

GC content: 43.45

Gene sequence:

>1818_bases
ATGAACAGTCAAGATTTAAAAAAACGTCAGGAGAAGATTCGTAATTTCTCCATTATTGCACATATTGACCATGGCAAATC
TACTCTCGCTGACCGCATTTTGGAAAAGACAGAGACGGTTTCGTCTCGTGAAATGCAGGCTCAGTTATTGGACTCCATGG
ATTTGGAGCGTGAGCGTGGGATTACCATTAAGTTAAACGCCATTGAGCTTAATTACACGGCTAAAGATGGGGAAACCTAC
ATTTTCCACCTTATTGATACCCCAGGGCATGTGGACTTTACTTATGAAGTATCGCGTTCGTTGGCAGCCTGTGAGGGAGC
GATTTTAGTTGTGGATGCAGCGCAGGGCATTGAAGCGCAGACCCTTGCCAATGTTTACCTAGCTCTTGATAATGATTTGG
AAATTTTACCGGTTATCAATAAAATTGATTTGCCTGCTGCTGACCCTGAGAGAGTCCGCCATGAAGTAGAAGATGTCATT
GGACTTGATGCTTCTGAGGCTGTTCTGGCGTCAGCCAAGGCTGGTATTGGGATCGAAGAGATTCTTGAGCAAATTGTTGA
GAAGGTTCCTGCTCCTACGGGTGATGTGGATGCGCCTTTACAGGCCTTGATTTTTGACTCTGTGTATGATGCTTACCGAG
GGGTAATTTTGCAGGTTCGGATTGTGAACGGTATTGTCAAGCCTGGCGATAAAATCCAGATGATGTCGAATGGAAAAACC
TTTGATGTTACAGAAGTTGGGATTTTCACTCCTAAGGCAGTTGGACGAGATTTCCTTGCGACGGGAGATGTTGGTTATGT
TGCGGCTTCTATCAAAACGGTAGCAGATACCCGTGTGGGGGATACGGTGACTTTAGCTAATAACCCTGCTAAGGAAGCCT
TGCATGGCTACAAGCAGATGAACCCGATGGTCTTTGCAGGGATTTATCCGATTGAATCAAATAAATACAATGACTTGCGT
GAAGCGCTTGAAAAATTACAACTGAACGATGCCAGTTTACAGTTTGAACCCGAAACGTCACAAGCGCTTGGCTTTGGTTT
TCGATGCGGCTTTTTAGGTTTGCTTCACATGGACGTGATTCAAGAACGTTTAGAGCGTGAATTTAACATTGATTTGATCA
TGACAGCACCGTCCGTGGTGTATCACGTTCACACAACAGATGAGGATATGATTGAAGTGTCTAACCCTTCAGAATTTCCA
GACCCAACCCGTGTGGCTTTTATTGAAGAGCCTTATGTTAAAGCGCAAATCATGGTGCCACAAGAGTTCGTAGGGGCTGT
TATGGAATTATCGCAGCGCAAGCGTGGTGATTTTGTGACCATGGATTACATTGATGATAACCGTGTCAATGTGATTTACC
AAATCCCGCTGGCTGAAATTGTCTTTGATTTCTTTGATAAATTGAAATCCTCAACGCGAGGCTATGCGAGTTTTGATTAT
GACATGTCAGAATACCGCAGGTCACAATTGGTGAAAATGGACATTCTTTTGAACGGTGATAAGGTCGATGCGCTCAGCTT
TATTGTTCATAAAGAATTTGCTTATGAACGCGGGAAAATTATTGTTGAGAAATTGAAAAAAATCATTCCACGTCAACAGT
TTGAAGTGCCGATCCAAGCCGCTATTGGTCAAAAAATTGTTGCGCGTTCAGACATCAAGGCCCTTCGTAAAAACGTTTTA
GCGAAGTGTTATGGCGGTGACGTGTCTCGTAAACGTAAATTGCTCGAAAAACAAAAGGCTGGTAAAAAACGGAATGAAGG
CTATTGGTTCTGTCGAAGTCCCTCAAGAAGCCTTCTTGAGTGTTCTTTCAATGGATGA

Upstream 100 bases:

>100_bases
CTCCGCTATTCTCTTGCAAGATAATCCGTAAGAAAAGATAGGCAAAGGCCTACCTTTTTTGTTATAATAGAAGGAAAGAC
TAAATGTATAGGAAAATAGA

Downstream 100 bases:

>100_bases
CGATACCAAGAAATAACCAAGCTTAATCGCTTAGTCTTCAGAGGAGAACTCTTGTGGGTCTACATGACCAAAAAATTGAT
TATCTCATAAAAAAAAGAAC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERGITIKLNAIELNYTAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQVRIVNGIVKPGDKIQMMSNGKT
FDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVADTRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLR
EALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIEVSNPSEFP
DPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDY
DMSEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVL
AKCYGGDVSRKRKLLEKQKAGKKRNEGYWFCRSPSRSLLECSFNG

Sequences:

>Translated_605_residues
MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERGITIKLNAIELNYTAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQVRIVNGIVKPGDKIQMMSNGKT
FDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVADTRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLR
EALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIEVSNPSEFP
DPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDY
DMSEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVL
AKCYGGDVSRKRKLLEKQKAGKKRNEGYWFCRSPSRSLLECSFNG
>Mature_605_residues
MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERGITIKLNAIELNYTAKDGETY
IFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQVRIVNGIVKPGDKIQMMSNGKT
FDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVADTRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLR
EALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIEVSNPSEFP
DPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDY
DMSEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVL
AKCYGGDVSRKRKLLEKQKAGKKRNEGYWFCRSPSRSLLECSFNG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=582, Percent_Identity=50, Blast_Score=594, Evalue=1e-170,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=44.5945945945946, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=38.7755102040816, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI18390331, Length=161, Percent_Identity=37.2670807453416, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI310132016, Length=125, Percent_Identity=42.4, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI310110807, Length=125, Percent_Identity=42.4, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI310123363, Length=125, Percent_Identity=42.4, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI217272892, Length=168, Percent_Identity=33.9285714285714, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI217272894, Length=168, Percent_Identity=33.9285714285714, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI25306283, Length=135, Percent_Identity=42.962962962963, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI19923640, Length=135, Percent_Identity=42.962962962963, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=42.962962962963, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI53729339, Length=252, Percent_Identity=27.3809523809524, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729337, Length=252, Percent_Identity=27.3809523809524, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788922, Length=574, Percent_Identity=58.1881533101045, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=505, Percent_Identity=31.2871287128713, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1789738, Length=183, Percent_Identity=34.4262295081967, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=298, Percent_Identity=27.8523489932886, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789559, Length=256, Percent_Identity=29.296875, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790412, Length=265, Percent_Identity=27.9245283018868, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789737, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789108, Length=158, Percent_Identity=29.1139240506329, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=594, Percent_Identity=42.2558922558923, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71988811, Length=181, Percent_Identity=35.3591160220994, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=181, Percent_Identity=35.3591160220994, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=39.7260273972603, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556745, Length=161, Percent_Identity=34.7826086956522, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=34.5679012345679, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=153, Percent_Identity=31.3725490196078, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=268, Percent_Identity=31.7164179104478, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=148, Percent_Identity=30.4054054054054, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=581, Percent_Identity=45.0946643717728, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6323098, Length=185, Percent_Identity=37.8378378378378, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=153, Percent_Identity=38.562091503268, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=153, Percent_Identity=38.562091503268, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=152, Percent_Identity=37.5, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=36.1702127659575, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=258, Percent_Identity=28.2945736434109, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=29.0780141843972, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=597, Percent_Identity=43.21608040201, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24582462, Length=186, Percent_Identity=39.247311827957, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=41.6666666666667, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=33.7662337662338, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=33.7748344370861, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=39.3333333333333, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357743, Length=170, Percent_Identity=32.9411764705882, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI281363316, Length=270, Percent_Identity=27.4074074074074, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864358, Length=270, Percent_Identity=27.4074074074074, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921738, Length=230, Percent_Identity=30, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28572034, Length=225, Percent_Identity=28, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67805; Mature: 67805

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERG
CCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
ITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVIGLDASEAVLASAKAGIGIEE
HHEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQVRIVNGIVKPGDKIQMMSNGKT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCE
FDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVADTRVGDTVTLANNPAKEALHGYKQM
EEEEEECCCCCHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHC
NPMVFAGIYPIESNKYNDLREALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVI
CCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHH
QERLEREFNIDLIMTAPSVVYHVHTTDEDMIEVSNPSEFPDPTRVAFIEEPYVKAQIMVP
HHHHHHHCCEEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECC
QEFVGAVMELSQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDY
HHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECC
DMSEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKIIVEKLKKIIPRQQFEVPIQA
CHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
AIGQKIVARSDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRNEGYWFCRSPSRSLLE
HHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEE
CSFNG
EECCC
>Mature Secondary Structure
MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSREMQAQLLDSMDLERERG
CCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
ITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQ
EEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHH
TLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVIGLDASEAVLASAKAGIGIEE
HHEEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
ILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQVRIVNGIVKPGDKIQMMSNGKT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEECCCCE
FDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVADTRVGDTVTLANNPAKEALHGYKQM
EEEEEECCCCCHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHC
NPMVFAGIYPIESNKYNDLREALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVI
CCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCHHHHHHHHHHHHHH
QERLEREFNIDLIMTAPSVVYHVHTTDEDMIEVSNPSEFPDPTRVAFIEEPYVKAQIMVP
HHHHHHHCCEEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEECC
QEFVGAVMELSQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFDY
HHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECC
DMSEYRRSQLVKMDILLNGDKVDALSFIVHKEFAYERGKIIVEKLKKIIPRQQFEVPIQA
CHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
AIGQKIVARSDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRNEGYWFCRSPSRSLLE
HHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEE
CSFNG
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA