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The map label for this gene is acoA [H]

Identifier: 94994299

GI number: 94994299

Start: 855521

End: 856501

Strand: Direct

Name: acoA [H]

Synonym: MGAS10750_Spy0903

Alternate gene names: 94994299

Gene position: 855521-856501 (Clockwise)

Preceding gene: 94994298

Following gene: 94994300

Centisome position: 44.16

GC content: 41.9

Gene sequence:

>981_bases
ATGGAGGCCGAAATGGTAACAGTTTCTAAAGAACAACATTTGGATATGTTCCTTAAAATGGAACGTATTCGTGAATTTGA
TTCACGTATTAATAAACTCGTCCGTCGTGGATTTGTACAAGGGATGACTCACTTCTCAGTTGGTGAAGAAGCAGCCAATG
TTGGAGCGGTGGCTCACTTGTCTTACGATGACATTATTTTTTCAAATCACCGTGGACATGGACAATCAATCGCTAAAGAC
ATGGACCTTAATAAAATGATGGCTGAGCTTGCTGGTAAAGCAACTGGTGTTTCTAAAGGGCGTGGTGGCTCAATGCACTT
GGCTGATTTTGAAAAAGGAAACTATGGTACCAATGGTATCGTTGGTGGTGGTTACGCTCTTGCAGTTGGTGCTGCCCTCA
CCCAACAATACAAAGGAACTAACAATATAGCTGTTGCTTTCTCAGGTGATGGCGCTACTAACGAAGGATCATTCCACGAG
TCTGTTAATATGGCTGCAACATGGAAACTTCCTGTTATTTTCTTCATCATCAACAACCGTTATGGAATTTCAATGAGCAT
CAATAATGCAACAAATACACCACACTTATACACACGTGCAGAAGCTTACGGAATTCCTGGTTTCTACTGTGAAGATGGCA
ATGACGTGATGGCTGTCTATGAAACAATGGGCAAAGCTGTTGAGCATGTTCGTGGTGGAAATGGCCCAGCTATTGTTGAG
GTAGAATCATACCGTTGGTTTGGTCACTCGACTGCAGATGCTGGTAAATACCGTACTAAAGAAGAAGTAGATGAGTGGAA
AGAAAAAGATCCAATGATCAAATATCGTACTTATCTGACAAGCGAAGGCATTGCCACTGATGACGAATTGGATGCTATTC
AAGCACAAGTCAAAAAAGAAGTTGACGATGCTTATGAATTTGCACAAAACAGTCCAGATCCAGAACTTTCAGTAGCCTTC
GAGGACGTTTGGGTTGACTAA

Upstream 100 bases:

>100_bases
TATTGCATTTCTAAAAAACCAAATAAGGTAAGGTAGAAAGCGATTTAATTATTTGCAAATTGTGAAAAAAAGTTTTATCA
TAAAAAGGTAATATGATAAT

Downstream 100 bases:

>100_bases
GATCACCTTGTCAGTAAGAAGCAATCGTATTTGAATAATAAATGGAGAAGTAAAAATGATGTCAGAAACAAAATTAATGG
CTTTGCGTGAAGCAGTCAAC

Product: Pyruvate dehydrogenase E1 component alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MEAEMVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKD
MDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHE
SVNMAATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVE
VESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAF
EDVWVD

Sequences:

>Translated_326_residues
MEAEMVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKD
MDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHE
SVNMAATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVE
VESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAF
EDVWVD
>Mature_326_residues
MEAEMVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHLSYDDIIFSNHRGHGQSIAKD
MDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHE
SVNMAATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVE
VESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAF
EDVWVD

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=312, Percent_Identity=38.1410256410256, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI4505685, Length=312, Percent_Identity=36.2179487179487, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI291084742, Length=312, Percent_Identity=36.2179487179487, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI291084744, Length=319, Percent_Identity=35.423197492163, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI291084757, Length=312, Percent_Identity=32.6923076923077, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=31.25, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI258645172, Length=320, Percent_Identity=31.25, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32564172, Length=307, Percent_Identity=36.1563517915309, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17536047, Length=307, Percent_Identity=36.1563517915309, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI86563355, Length=315, Percent_Identity=32.3809523809524, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI86563357, Length=315, Percent_Identity=32.3809523809524, Blast_Score=144, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=37.5776397515528, Blast_Score=215, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24639744, Length=326, Percent_Identity=38.0368098159509, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28571106, Length=326, Percent_Identity=38.0368098159509, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24639740, Length=326, Percent_Identity=38.0368098159509, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24639746, Length=309, Percent_Identity=38.5113268608414, Blast_Score=203, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639748, Length=321, Percent_Identity=35.202492211838, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI21355903, Length=316, Percent_Identity=27.5316455696203, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35919; Mature: 35919

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAEMVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCEEEEE
SYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGI
CCCCEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNMAATWKLPVIFFIINNR
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCEEEEECCEEEEEEEECC
YGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVE
EEEEEEECCCCCCCEEEEEHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKE
EECEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
VDDAYEFAQNSPDPELSVAFEDVWVD
HHHHHHHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MEAEMVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVGEEAANVGAVAHL
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCEEEEE
SYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMHLADFEKGNYGTNGI
CCCCEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNMAATWKLPVIFFIINNR
ECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCEEEEECCEEEEEEEECC
YGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYETMGKAVEHVRGGNGPAIVE
EEEEEEECCCCCCCEEEEEHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLTSEGIATDDELDAIQAQVKKE
EECEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
VDDAYEFAQNSPDPELSVAFEDVWVD
HHHHHHHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]