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The map label for this gene is dnaG [H]

Identifier: 94994082

GI number: 94994082

Start: 661447

End: 663327

Strand: Direct

Name: dnaG [H]

Synonym: MGAS10750_Spy0686

Alternate gene names: 94994082

Gene position: 661447-663327 (Clockwise)

Preceding gene: 94994081

Following gene: 94994083

Centisome position: 34.15

GC content: 38.01

Gene sequence:

>1881_bases
GTGATCACTTCAGTTTTTAAATTTACAAAAAAGAGTTTCGGTGGTATAATAAAACGTAAAACTAAAATGGGATTGTTATG
GGGAGGTGACGATTTGGCAATTGACAAAGAAATGATTTCCCAAGTAAAAAATAGCGTTAATATTGTCGATGTCATTGGAG
AAGTGGTCAAACTTTCCCGATCAGGGCGGCATTATCTCGGGCTTTGCCCATTTCATAAGGAAAAGACACCCTCTTTTAAT
GTTGTTGAAGACAGACAATTTTTTCACTGCTTTGGCTGTGGAAAATCAGGGGATGTTTTTAAATTTATTGAGGAATACCG
CCAAGTCCCCTTCTTAGAAAGTGTTCAGATTATTGCCGATAAGACTGGTATGTCGCTTAATATCCCGCCAAGTCAGGCAG
TACTTGCTAGCCAACACAAGCACCCTAATCACGCTTTGATGACACTTCATGAGGATGCTGCTAAATTTTACCATGCAGTT
TTGATGACCACTACCATTGGTCAAGAAGCTAGGAAGTACCTTTACCAGAGAGGTTTGGATGACCAATTAATTGAGCATTT
CAATATTGGTTTAGCCCCAGATGAGTCAGATTATCTTTATCAAGCTCTTTCTAAAAAATACGAGGAAGGTCAATTGGTTG
CTTCAGGATTGTTTAACTTGTCCGATCAATCTAATACCATTTACGACGCCTTTCGAAATCGTATCATGTTTCCCTTATCA
GATGACCGAGGGCATATTATTGCCTTTTCAGGACGTATCTGGACGGCAGCTGATACAGAAAAGAGACAGGCCAAGTATAA
AAATTCAAGAGGAACAGTTCTTTTTAACAAATCTTATGAATTGTATCATCTGGACAAGGCAAGGCCTGTTATTGCCAAAA
CCCATGAAGTATTTCTAATGGAAGGGTTTATGGACGTGATTGCCGCTTACCGTTCCGGCTATGAAAATGCTGTTGCTTCA
ATGGGGACGGCATTGACTCAAGAACATGTCAATCACCTTAAGCAAGTCACTAAAAAAGTTGTTTTGATTTATGATGGTGA
CGATGCTGGACAACATGCTATTGCAAAATCACTAGAATTGCTTAAAGATTTTGTTGTCGAAATTGTCAGAATCCCCAATA
AAATGGATCCTGACGAATTTGTACAACGGCATTCCCCAGAAGCATTTGCAGATTTGCTTAAGCAGTCACGGATCAGTAGT
GTTGAATTTTTTATTGATTACCTAAAACCTACTAATGTAGACAATTTGCAATCACAAATTGTTTATGTGGAGAAAATGGC
ACCATTGATTGCTCAATCACCATCCATCACAGCTCAACATTCGTATATTAACAAGATTGCTGATTTGTTGCCAAACTTTG
ACTATTTTCAAGTAGAACAATCAGTAAATGCATTAAGGATTCAAGATAGGCAAAAGCGTCAAGGTCAAATAGCTCAAGCT
GTCAGCAATCTTGTGACCTTACCAATGCCAAAAAGTTTGACAGCTATTGCTAAGACAGAAAGTCATCTCATGCATCGGCT
CTTACATCATGACTATTTATTAAATGAATTTCGACATCGTGATGATTTTTATTTTGATACCTCTACCTTAGAATTACTTT
ATCAACGGCTGAAGCAACAAGGACACATTACATCTTATGATTTGTCAGAGATGTCGGAGGAAGTTAACCGTGCTTATTAT
AATGTTTTAGAAGAAAACCTTCCCAAAGAAGTAGCTCTTGGTGAGATTGATGATATTTTATCCAAACGTGCCAAACTTTT
AGCAGAGCGCGATCTTCACAAACAAGGGAAAAAAGTTAGAGAATCTAGTAATAAAGGCGATCATCAAGCGGCTCTAGAAG
TACTAGAACATTTTATTGCGCAGAAACGAAAAATGGAATAG

Upstream 100 bases:

>100_bases
TGGAGAAAGAGGCTAAGCAGATGATTGATTTAAAAAGGAATCTTTTCAAAATTGATGTCCGGGCACAAAAGGATGAAGAG
AAAGTTTTCATGCGTACGGC

Downstream 100 bases:

>100_bases
AGGAAATTATGACAAAACAAAAAGAAATAACAACTTTTAACGTTCAAGTTGCGGAGTTTATTCGCCATCACAAAAAAGAA
GGAACAGCGATTGATGATGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MITSVFKFTKKSFGGIIKRKTKMGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFN
VVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAV
LMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVAS
MGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISS
VEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQA
VSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYY
NVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME

Sequences:

>Translated_626_residues
MITSVFKFTKKSFGGIIKRKTKMGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFN
VVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAV
LMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVAS
MGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISS
VEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQA
VSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYY
NVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME
>Mature_626_residues
MITSVFKFTKKSFGGIIKRKTKMGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYLGLCPFHKEKTPSFN
VVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAV
LMTTTIGQEARKYLYQRGLDDQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLMEGFMDVIAAYRSGYENAVAS
MGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISS
VEFFIDYLKPTNVDNLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQA
VSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQGHITSYDLSEMSEEVNRAYY
NVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=434, Percent_Identity=32.9493087557604, Blast_Score=233, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71601; Mature: 71601

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSVFKFTKKSFGGIIKRKTKMGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSR
CCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCHHHHHHHHHCCHHHHHHHHHHHHHC
SGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIAD
CCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCC
KTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLD
CCCCEEECCCCHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCEECEEC
DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLM
CCCCCEEEECCEEEECCCCHHHHHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHH
EGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLEL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHH
LKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQI
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQ
HHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHC
GHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSNKGDHQAALEVLEHFIAQKRKME
HCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITSVFKFTKKSFGGIIKRKTKMGLLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSR
CCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHCCHHHHHHHHHCCHHHHHHHHHHHHHC
SGRHYLGLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIAD
CCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHCC
KTGMSLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLD
CCCCEEECCCCHHHHHHCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DQLIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCEECEEC
DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVFLM
CCCCCEEEECCEEEECCCCHHHHHHHCCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHH
EGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAKSLEL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHH
LKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVDNLQSQI
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQKRQGQIAQA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTLELLYQRLKQQ
HHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHC
GHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLAERDLHKQGKKVR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESSNKGDHQAALEVLEHFIAQKRKME
HCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471; 7765979; 7503808 [H]