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The map label for this gene is ligA [H]

Identifier: 94994051

GI number: 94994051

Start: 630112

End: 632070

Strand: Direct

Name: ligA [H]

Synonym: MGAS10750_Spy0655

Alternate gene names: 94994051

Gene position: 630112-632070 (Clockwise)

Preceding gene: 94994050

Following gene: 94994052

Centisome position: 32.53

GC content: 40.63

Gene sequence:

>1959_bases
ATGAAAAAACGCATAAAAGAATTAACAGATCTTCTTAACCGTTACCGTTATGACTATTATACTAAAGATGCCCCATCAGT
TTCTGACAGTGACTATGATAAGCTTTATCGTGAACTGGTGACTTTAGAGCAGTCATATCCCGAGTATGTCTTGCAAGATA
GCCCAACGCAGCAGGTTGGAGGGACTATTTTAAAGGGGTTTGAAAAATATCGACATCAGTATCCGCTTTTTAGTTTGCAG
GATGCTTTTTCACGTGAGGAGCTGGATGCTTTTGATAAGCGCGTCAAGGCCGAATTTCCGAATGCCACCTATCTAGCAGA
GTTAAAAATTGATGGTTTATCTATTTCCTTGAGTTATGAGAATGGCTTCTTACAGGTTGGAGCAACACGGGGTGATGGCA
ATATCGGTGAAAATATCACAGAAAATATCAAAAAAATAAAGGATATTCCTCACCAATTGAGTGAGCCTTTAACCATCACC
GTTCGAGGAGAGGCTTACATGTCTCGTCAGTCCTTTAAAGCGATCAATGAAGCACGCCAAGAAAATGGGGAGACTGAATT
TGCTAATCCTAGAAATGCTGCCGCTGGGACACTAAGGCAGCTAGATACGGCAGTGGTTGCTAAGCGTCAACTAGCCACTT
TTTTGTATCAAGAAGCTAGTCCGACAGCTAGAAATCAACAAAATGAGGTCTTAGCAGAATTAGCAGATCTAGGTTTTTCA
GTAAATCCGTATTATCAACTCACATCGTCTATGGATGAGATTTGGGATTTTATCAAAACTATTGAGGCAAAAAGAGATCA
ATTAGCTTATGACATAGATGGCGTTGTTATTAAAGTTAATAGCCTAGCCATGCAAGAAGAGCTAGGGTTTACGGTTAAAG
CTCCACGCTGGGCCATTGCTTACAAATTTCCAGCAGAAGAAAAAGAGGCAGAAATCCTTTCGGTTGACTGGACAGTGGGA
CGAACAGGCGTGGTCACACCTACAGCGAATCTAACTCCTGTGCAACTAGCGGGGACAACCGTGAGTCGTGCAACCCTACA
TAATGTGGATTATATTGCCGAAAAAGATATCCGTATTGGCGATACAGTGATTGTATATAAGGCAGGGGACATTATTCCTG
CTGTCTTAAATGTCGTTATGAGTAAGCGGAACCAGCAAGAAGTCATGCTTATTCCTAAACTATGCCCATCTTGTGGTAGT
GAATTAGTTCATTTTGAGGATGAGGTTGCTTTGCGTTGTATTAATCCGTTGTGTCCTAGTTTGATTCAGCGAAGTTTGGA
GCATTTTGCAAGTCGTGATGCCATGAACATTACTGGGCTGGGACCAGCTATTGTGGAAAAACTATTTTTAGCGGGGTTTG
TTCATGATGTTGCTGATATTTACCAGTTGACCAAAGAAAATTTCATGCAATTAGATGGCATCAAAGAAAAGTCAGCTGAT
AAATTGCTCGCTGCGATTGAGGCTTCTAAATCAAATTCAGCTGAAAAACTGTTATTTGGTCTAGGAATTCGTCATATTGG
CTCTAAAGTTAGTCGCTTAATTTTAGAGGTATACGGAGATATTTCTGCTCTTTTAACCGCTAAAGAAGAGGAAATCGCAA
GGATAGACGGCCTTGGCTCAACGATTGCTCAATCACTTACTCAATACTTTGAACAAAAAACGGCTGCGATTTTAGTAGAC
GAGTTAAAGACAGCAGGTGTCAACATGCATTACTCAGGTCAAAAAGTTAATAGTGACGCAGCGCTTTTTGGTCTAACTGT
TGTTTTAACTGGCAAATTAAATCAGCTGAACCGAAATGAGGCCAAAGATAAATTAGAGGCATTAGGTGCCAAAGTTACTG
GAAGTGTATCTAAGAAAACAGATTTAGTTATTGCAGGTAGTGACGCTGGTTCTAAGCTAGAAAAAGCAAAGAGTTTAGGA
ATTCGGATTGAAGATGAAGATTGGCTACGCCAGCTTTAA

Upstream 100 bases:

>100_bases
CTTTAGCTAATAGTACCATATGGAGTGGCCAACGATTACTTTTGATGTTGTTATGATATAGAAATAGCTCATTTGTTATC
TTTTATGATAAAATAAAAGT

Downstream 100 bases:

>100_bases
GTGAGGTAATGTTATGAAAAAACAATTGAGAGCACGTCTCATTTATAATCCAACGTCAGGACAAGAACTTATGAGAAAAA
GTGTTCCTGAAGTTTTAGAC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKENFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Sequences:

>Translated_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKENFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL
>Mature_652_residues
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQ
DAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGS
ELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKENFMQLDGIKEKSAD
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVD
ELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLG
IRIEDEDWLRQL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=42.7503736920777, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082305, Length=558, Percent_Identity=22.7598566308244, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 72378; Mature: 72378

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTITVRGEAYMSRQSFKAINEARQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKENFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC
>Mature Secondary Structure
MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQSYPEYVLQDSPTQQVG
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
GTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNATYLAELKIDGLSISLSYE
HHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEC
NGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTITVRGEAYMSRQSFKAINEARQ
CCEEEEEEECCCCCCCCHHHHHHHHHHHCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHH
ENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQEASPTARNQQNEVLAELADLGFS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCC
VNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVVIKVNSLAMQEELGFTVKAPRWAIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEHHHHHHHCCCEEECCCEEEE
YKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRIG
EECCCCCCCCCEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCCHHHHCCCCEEC
DTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIPKLCPSCGSELVHFEDEVALRCINPLCPS
CEEEEEECCCHHHHHHHHHHHCCCCCCEEEEHHHCHHHCCHHEECCHHHHHHHHHHHHHH
LIQRSLEHFASRDAMNITGLGPAIVEKLFLAGFVHDVADIYQLTKENFMQLDGIKEKSAD
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KLLAAIEASKSNSAEKLLFGLGIRHIGSKVSRLILEVYGDISALLTAKEEEIARIDGLGS
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHH
TIAQSLTQYFEQKTAAILVDELKTAGVNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHEEEEEEEECCCHHCCHHH
AKDKLEALGAKVTGSVSKKTDLVIAGSDAGSKLEKAKSLGIRIEDEDWLRQL
HHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]