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The map label for this gene is glpF [H]

Identifier: 94993846

GI number: 94993846

Start: 438098

End: 438808

Strand: Direct

Name: glpF [H]

Synonym: MGAS10750_Spy0450

Alternate gene names: 94993846

Gene position: 438098-438808 (Clockwise)

Preceding gene: 94993845

Following gene: 94993847

Centisome position: 22.62

GC content: 43.88

Gene sequence:

>711_bases
ATGAATTTTATCGGTGAACTGTTAGGAACGTTTATTTTGGTTTTATTAGGAGACGGTGTGGTTTCTGCTTGTATCTTGAA
TAAAACCAAAGCTCAAAATAGTGGCTGGATTGCTATTGTTTTGGGCTGGGGAATTGCTGTAACCGTTGCGGTTTATATTA
GTGGTTTCATGAGTGGTGCCCATTTAAACCCAGCCGTGACACTCGCTATGGCAGCCATTGGCTCATTGCCATGGTCTCAG
GTAGTGACTTATCTTGTGGCTCAATTTCTTGGAGCAATGCTTGGAGCCTTAGTTCTCTACTTGCATTACTACCCACATTG
GAAGGAAACCAAAGATGCTGGTACGATTTTAGCTTGTTTCTCAACTGGGCCAGCTATTCGCCACGCTTGGTCAAACCTTT
TGGGCGAAGCTTTAGGAACAGCTGTTTTAGTGATTACGGTAATGGCCATTGGCCCTAATAAAGTAGCTGCTGGTTTTGGT
CCGATTATTGTTGGTTTTGTGGTTATGGCAGTTGGTTTTTCACTTGGAGCAACCACTGGTTATGCCATCAACCCAGCGCG
TGACCTTGGGCCACGTATCATGCATGCCTTACTTCCAATCCCAAATAAAGGAGATTCTGACTGGTCATACGCTTGGATTC
CAGTTTTAGGTCCAATCCTTGGTGGGGTCGTAGGTGCCTTGATTTATCAAGTGATTTTAAATATGATGTAA

Upstream 100 bases:

>100_bases
ACACAGCAGCAGCTCTTATTCAGGCTGGCGCGGACAAGGATGCTATTTTAAGTCAGTTAGAAGAGTTAGCTATCAATAAA
TAAATAGCAAGGAGATTATC

Downstream 100 bases:

>100_bases
TGAACCAAAAAAAGTTGGGTCTAGGACTCAACTTTTTTTGGTGGCTAAGAAGTGTTCTTTTACAGATTTTCCCTTTTCTT
GGACCGGAGCTTTCAGTATA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWGIAVTVAVYISGFMSGAHLNPAVTLAMAAIGSLPWSQ
VVTYLVAQFLGAMLGALVLYLHYYPHWKETKDAGTILACFSTGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFG
PIIVGFVVMAVGFSLGATTGYAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGPILGGVVGALIYQVILNMM

Sequences:

>Translated_236_residues
MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWGIAVTVAVYISGFMSGAHLNPAVTLAMAAIGSLPWSQ
VVTYLVAQFLGAMLGALVLYLHYYPHWKETKDAGTILACFSTGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFG
PIIVGFVVMAVGFSLGATTGYAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGPILGGVVGALIYQVILNMM
>Mature_236_residues
MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWGIAVTVAVYISGFMSGAHLNPAVTLAMAAIGSLPWSQ
VVTYLVAQFLGAMLGALVLYLHYYPHWKETKDAGTILACFSTGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFG
PIIVGFVVMAVGFSLGATTGYAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGPILGGVVGALIYQVILNMM

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=247, Percent_Identity=36.0323886639676, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI4826645, Length=246, Percent_Identity=37.8048780487805, Blast_Score=138, Evalue=5e-33,
Organism=Homo sapiens, GI22538420, Length=250, Percent_Identity=33.6, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI4502187, Length=247, Percent_Identity=34.0080971659919, Blast_Score=127, Evalue=8e-30,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=32.5966850828729, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=32.5966850828729, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI4502181, Length=204, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4755125, Length=204, Percent_Identity=29.4117647058824, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI6912506, Length=204, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI45446752, Length=209, Percent_Identity=28.7081339712919, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790362, Length=247, Percent_Identity=32.3886639676113, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=238, Percent_Identity=36.1344537815126, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17533613, Length=246, Percent_Identity=34.1463414634146, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564052, Length=246, Percent_Identity=34.1463414634146, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=31.4516129032258, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=31.6872427983539, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=31.6872427983539, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=31.8548387096774, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=239, Percent_Identity=32.2175732217573, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558372, Length=205, Percent_Identity=30.2439024390244, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71993722, Length=231, Percent_Identity=28.1385281385281, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=207, Percent_Identity=35.7487922705314, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=25.5172413793103, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652747, Length=237, Percent_Identity=30.379746835443, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45551084, Length=237, Percent_Identity=30.379746835443, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24762342, Length=239, Percent_Identity=29.2887029288703, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762344, Length=240, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24762346, Length=240, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24762348, Length=240, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130305, Length=240, Percent_Identity=31.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17136672, Length=199, Percent_Identity=31.6582914572864, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922038, Length=232, Percent_Identity=28.8793103448276, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652751, Length=233, Percent_Identity=28.755364806867, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550503, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24707; Mature: 24707

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWGIAVTVAVYISGFMSGA
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
HLNPAVTLAMAAIGSLPWSQVVTYLVAQFLGAMLGALVLYLHYYPHWKETKDAGTILACF
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
STGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFGPIIVGFVVMAVGFSLGATTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGPILGGVVGALIYQVILNMM
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFIGELLGTFILVLLGDGVVSACILNKTKAQNSGWIAIVLGWGIAVTVAVYISGFMSGA
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
HLNPAVTLAMAAIGSLPWSQVVTYLVAQFLGAMLGALVLYLHYYPHWKETKDAGTILACF
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
STGPAIRHAWSNLLGEALGTAVLVITVMAIGPNKVAAGFGPIIVGFVVMAVGFSLGATTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
YAINPARDLGPRIMHALLPIPNKGDSDWSYAWIPVLGPILGGVVGALIYQVILNMM
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]