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The map label for this gene is nrdE1 [H]

Identifier: 94993747

GI number: 94993747

Start: 356045

End: 358225

Strand: Direct

Name: nrdE1 [H]

Synonym: MGAS10750_Spy0351

Alternate gene names: 94993747

Gene position: 356045-358225 (Clockwise)

Preceding gene: 94993746

Following gene: 94993748

Centisome position: 18.38

GC content: 45.67

Gene sequence:

>2181_bases
ATGTCACAAACCAATGCGTCATACCTCTCATTAAATGCACTGACTCGTTTTAAAAAACCAGACGGCAGCTACCATTTTGA
TAGCGATAAAGAAGCTGTTAGGCGCTACCTGGAAGAACATGTCTCTCCCAATCAAATGGCTTTTAACTCACTAGAAGATA
AGCTAGCTTACCTCATCAATGAAGGGTATTATGAGCAGGCCATTTTTGATGCCTATCCTAACGACCTTATCAAGGAGGCC
TTTCATTATGCCTATCAACAAGGCTATCGCTTTTTAAATCTCATGGGCGCTATGAAATTTTACCAGTCCTATGCCTTAAA
AACCTTGGACGGTAAGCATTACCTAGAAACATTTGAAGACCGTGCTGTGATGAATGCACTCTTTTTAGCTGATGGAGATC
AAACCTTTGTCTTTGACGTGATTGATGCCATCTTGCACCGACGCTTTCAACCAGCTACCCCAACCTTTTTAAATGCCGGA
AAAAAACGCCGTGGCGAATACATTTCGTGCTATCTTCTTCGTGTCGAAGACAATATGGAATCTATCTCTCGCGCCATTAG
CACCAGCTTGCAACTCTCAAAAAGAGGAGGCGGAGTCGCCTTGTGCCTCACCAACCTCAGAGAAATCGGAGCACCGATAA
AAGGGATTGAAAATCAGGCAACAGGTATCGTTCCTGTCATGAAACTACTAGAGGACTCTTTTTCCTATGCCAATCAACTA
GGTCAGCGCCAAGGAGCAGGTGCGGTTTACCTTCATGCCCATCACCCCGAGGTCCTGACCTTTTTAGACACCAAACGTGA
AAATGCCGATGAGAAAATTCGCATTAAGTCCCTAGCATTGGGACTTGTGATCCCAGATATTACCTTCCAGTTAGCCAAAG
AAAACAAAGACATGGCTCTCTTTTCACCCTATGATATTAAGCGTGCCTATGGCAAGGACATGTCAGACATCTCCATCACA
GAGGAATACGATAAACTGCTAGCCAATCCGGCCATCAAAAAGACCTACATCAGTGCTAGAAAGTTCTTTCAGTTAATTGC
AGAGCTACATTTTGAGTCAGGCTACCCTTACCTCTTGTTTGATGACACGGTCAATAAGCGCAACCCTCATGCCAAAAAAG
GCAGAATCGTCATGTCTAACCTGTGTTCTGAAATCGCTCAAGTCAGCACCCCAAGCACTTTCAAAGAAGATTTGAGTTTT
GAAACCATTGGAGAGGATATCTGCTGTAATTTAGGGTCTATCAATATTGCCCAGGCTATGGCAGATGCGCCACACTTTGA
ACAGCTCATCACAACGAGCATCAGAGCACTTGATCGTGTCTCTAGACTGTCTGACCTGAATTGTGCGCCTTCTGTTGAGA
CAGGCAATGCAGCCAACCATGCAGTAGGATTAGGCGCCATGAACCTACATGGGTTTTTGGCAACCAACCACATCTACTAT
GATACAAAAGAAGCCGTTGACTTTACAGATCTGTTTTTCCATGCCATGGCCTATTATGCTTTTAAAGCCTCTTGCCAACT
AGCCAAAGAAAAAGGCGCTTTTGCAGGATTTTCGTTATCAACCTATGCAGATGGCACTTATTTTGCCAAGTATTTGCAAG
AAGATGCCAAGCCACAAACAGCCAAGGTTGCCACATTGTTGCAAGATTACGGCTTCACATTGCCAACGGTGGCGGATTGG
CAAGCTCTAGTAGCAGATATCAAGCAGTTTGGCCTAGCCAATGCGCACTTGCTAGCGGTTGCTCCGACAGGCTCCATCAG
CTACTTATCATCATGCACTCCGAGTCTACAACCAGTCGTTGCTCCTGTTGAGGTCCGAAAAGAAGGTAGCCTGGGCAGGA
TTTACGTCCCTGCCTATCAGATTGACCAGGCTAACTATGCTTACTATGAACGTGGTGCCTATGAGGTCGGCCCAAAAGCC
ATCATCGACGTTGTCGCTGCTGCCCAAAAACACGTTGACCAAGCCATCTCATTGACCCTGTTTATGACCGATCAAGCGAC
CACACGTGACCTTAACCGCTCCTATATCCAAGCTTTTAAGCAAAACTGTGCATCGATCTATTATGTGCGAGTGAGGCAAG
ACGTCCTAGCAGGCAGCGAACAGTATGACGAGGATAGTCTTGTGACAGCTCCTGGAGCTAGTGATGAGACCACCACAGAG
TGTCATAGCTGTATGATCTAA

Upstream 100 bases:

>100_bases
ACAGATGTTAAAAAGGTACAGGCGATTTTTGCCCGCTTAAAGCATCACACACACGACAAGCAAAACAAACCAACAACCTG
ATTACAGAAAGGACACACCC

Downstream 100 bases:

>100_bases
CAGCTACTAGAAAGCTCTCGTGTCCCCTACCACATGCATAGGATCAGTGCGCTCGACCTTTCAAGACAAGCAAGCATCAG
CTCTTGCTTGTCTTTTTTTG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKHYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRLSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CHSCMI

Sequences:

>Translated_726_residues
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEA
FHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKHYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISIT
EEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSF
ETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRLSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADW
QALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKA
IIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
CHSCMI
>Mature_725_residues
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAF
HYAYQQGYRFLNLMGAMKFYQSYALKTLDGKHYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGK
KRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQLG
QRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITE
EYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFE
TIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRLSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYYD
TKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQ
ALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAI
IDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTEC
HSCMI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=590, Percent_Identity=24.406779661017, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=54.7788873038516, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=672, Percent_Identity=26.6369047619048, Blast_Score=183, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17555242, Length=657, Percent_Identity=23.7442922374429, Blast_Score=178, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320914, Length=589, Percent_Identity=25.2971137521222, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=23.950233281493, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17352481, Length=472, Percent_Identity=27.7542372881356, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80985; Mature: 80854

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKHYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRLSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CHSCMI
HHHCCC
>Mature Secondary Structure 
SQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPNQMAFNSLEDKLAYLIN
CCCCCHHEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
EGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQSYALKTLDGKHYLETFED
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAGKKRRGEYISCYLLRVEDNME
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
SISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIENQATGIVPVMKLLEDSFSYANQL
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKSLALGLVIPDITFQLAKENKDMAL
HHCCCCCEEEEEECCCCHHHHHHCCCCCCCCCEEHHHHHHHHEECCHHHHHHHCCCCEEE
FSPYDIKRAYGKDMSDISITEEYDKLLANPAIKKTYISARKFFQLIAELHFESGYPYLLF
ECCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
DDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPSTFKEDLSFETIGEDICCNLGSINIAQAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHCCHHHHCCCCCCHHHHHH
ADAPHFEQLITTSIRALDRVSRLSDLNCAPSVETGNAANHAVGLGAMNLHGFLATNHIYY
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCEECCCEEEECCEEE
DTKEAVDFTDLFFHAMAYYAFKASCQLAKEKGAFAGFSLSTYADGTYFAKYLQEDAKPQT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCHHHHHHHHHHCCCCHH
AKVATLLQDYGFTLPTVADWQALVADIKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVV
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCCCC
APVEVRKEGSLGRIYVPAYQIDQANYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTL
CCHHCCCCCCCCEEEECEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
FMTDQATTRDLNRSYIQAFKQNCASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTE
EEECCHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCEEECCCCCCCCHHH
CHSCMI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]