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The map label for this gene is metS [H]

Identifier: 94993743

GI number: 94993743

Start: 352079

End: 354079

Strand: Direct

Name: metS [H]

Synonym: MGAS10750_Spy0347

Alternate gene names: 94993743

Gene position: 352079-354079 (Clockwise)

Preceding gene: 94993742

Following gene: 94993744

Centisome position: 18.18

GC content: 44.08

Gene sequence:

>2001_bases
ATGATGAAAAAACCATTTTACATTACGACACCCATTTATTACCCATCTGGGAAATTACACATTGGTTCTGCTTATACAAC
TATTGCTTGTGATGTCTTGGCACGCTACAAGCGCTTGATGGGCCATGAGGTCTTTTACCTCACAGGGCTTGATGAGCACG
GACAAAAGATTCAAACCAAGGCAAAAGAAGCTGGTATTACACCTCAAACCTATGTTGATAACATGGCAAAGGATGTCAAA
GCCCTTTGGCAATTGCTAGATATTTCCTATGACACATTTATCCGGACAACGGACGATTACCACGAAGAGGTCGTTGCTGC
TGTCTTTGAAAAATTGCTGGCGCAAGACGACATTTACCTTGGCGAATACTCTGGTTGGTATTCTGTCTCTGATGAGGAAT
TTTTCACTGAAAGCCAATTAAAAGAAGTCTTTAGAGATGAAGACGGTCAGGTTATTGGAGGGATTGCCCCATCAGGTCAT
GAGGTCGAGTGGGTCTCTGAAGAATCCTACTTCCTACGCCTTAGCAAATACGCTGATCGTCTTGTGGCCTTCTTTAAAGA
GCGACCTGACTTTATCCAACCAGATGGTCGTATGAATGAAATGGTGAAAAACTTTATCGAGCCAGGCCTAGAAGATTTGG
CTGTTAGCCGTACTACCTTTACATGGGGTGTACCTGTTCCATCAGACCCAAAACACGTGGTTTATGTGTGGATTGATGCC
CTCTTAAATTACGCGACAGCTCTTGGTTATGGTCAAGCTAATCACGCTAACTTTGATAAATTCTGGAATGGAACGGTTTT
CCACATGGTTGGAAAAGATATCTTACGTTTTCATAGCATCTACTGGCCAATCTTACTCATGATGCTTGATTTACCAATGC
CAGACCGCTTAATTGCTCATGGTTGGTTTGTCATGAAAGACGGTAAAATGTCTAAATCAAAAGGCAATGTGGTCTATCCA
GAAATGTTAGTGGAGCGCTTTGGACTTGATCCTCTTCGTTATTACCTCATGCGTTCTTTGCCAGTTGGTTCAGACGGAAC
CTTTACCCCTGAAGATTATGTGGGTCGTATCAACTATGAGTTAGCCAACGACCTTGGTAATTTATTGAACCGTACCGTTG
CCATGATCAACAAGTATTTTGATGGCACGGTGCCAGCTTATGTTGACAATGGAACTGCCTTTGATGCTGACTTAAGTCAA
CTGATTGATGCTCAGCTAGCAGACTACCATAAGCACATGGAAGCGGTGGACTACCCACGCGCCTTAGAAGCCATTTGGAC
CATCATTGCTCGTACCAACAAATACATTGATGAGACAGCTCCTTGGGTGCTCGCTAAAGAAGATGGTGACAAGGCTCAAC
TAGCCAGTGTCATGGCACACTTGGCAGCTAGCTTGCGCGTGGTCGCTCATGTGATCCAACCCTTTATGATGGAAACATCA
GCTGCTATCATGGCCCAGCTTGGCTTAGCGCCTGTTTCTGACTTGTCAACACTTGCTCTAGCAGATTTTCCTGCCAATAC
AAAGGTCGTTGCCAAAGGCACACCGATTTTCCCACGTCTTGATATGGAAGCTGAGATTGACTACATCAAAGCCCAAATGG
GTGACAGCTCAGCTATTTCCCAAGAAAAAGAATGGGTCCCTGAAGAGGTTGCGCTCAAATCTGAAAAAGACGTCATCACA
TTTGAAACCTTTGATGCTGTGGAAATCCGTGTGGCAGAAGTCAAGGAAGTGTCAAAAGTGGAAGGTTCAGAAAAATTGCT
GCGCTTTAGAGTAGATGCAGGAGACGGGCAAGACCGTCAAATCTTGTCAGGTATTGCCAAATTTTACCCCAACGAACAAG
AATTAGTGGGCAAAAAACTACAAATTGTTGCTAACCTGAAACCGCGTAAAATGATGAAACAATACATCAGCCAAGGCATG
ATTTTATCAGCAGAGCATGGAGATCAGCTAACGGTCTTAACCGTTGATCCCTCTGTTCCAAATGGAAGTATCATTGGCTG
A

Upstream 100 bases:

>100_bases
TCCATTAGCCTTGAGGGTTGAATTTAAACAGGGAAAAACGAAATCCTACACTATGCATCTCAATCGTACTTTCATTTGAG
TATGGTTCTGAAAGGAAACT

Downstream 100 bases:

>100_bases
TGCTGACCTATTATTCAAAAAACCTAAGCAAGAACATGTGCTTAGGTTTTTTTGATTGCCTTCAAGAAAGACGAATAACG
AGATGGGTGATATGACATCA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETS
AAIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKQYISQGM
ILSAEHGDQLTVLTVDPSVPNGSIIG

Sequences:

>Translated_666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETS
AAIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKQYISQGM
ILSAEHGDQLTVLTVDPSVPNGSIIG
>Mature_666_residues
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVK
ALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
LLNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYP
EMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQ
LIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETS
AAIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVIT
FETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKQYISQGM
ILSAEHGDQLTVLTVDPSVPNGSIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=507, Percent_Identity=34.7140039447732, Blast_Score=285, Evalue=7e-77,
Organism=Homo sapiens, GI14043022, Length=588, Percent_Identity=27.7210884353741, Blast_Score=190, Evalue=3e-48,
Organism=Escherichia coli, GI1788432, Length=680, Percent_Identity=28.2352941176471, Blast_Score=267, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI212645096, Length=496, Percent_Identity=31.4516129032258, Blast_Score=253, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17540794, Length=571, Percent_Identity=26.7950963222417, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71992781, Length=384, Percent_Identity=29.9479166666667, Blast_Score=178, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321610, Length=479, Percent_Identity=32.5678496868476, Blast_Score=263, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=561, Percent_Identity=25.4901960784314, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=33.2665330661323, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24655311, Length=566, Percent_Identity=24.9116607773852, Blast_Score=169, Evalue=7e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75072; Mature: 75072

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTK
CCCCCEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYADR
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHH
LVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHH
LLNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAH
HHHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHH
LANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAIWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAIS
HHHHHHHCCCCCCCHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHC
QEKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQ
HHHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCHHEEEEEECCCCCCHHH
ILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKQYISQGMILSAEHGDQLTVLTVDPSVP
HHHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCC
NGSIIG
CCCCCC
>Mature Secondary Structure
MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEVFYLTGLDEHGQKIQTK
CCCCCEEEECCEECCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
AKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHEEVVAAVFEKLLAQDDIYL
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHHHHHHHHHHCCCCEEE
GEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGHEVEWVSEESYFLRLSKYADR
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHH
LVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVVYVWIDA
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEEEHHHHH
LLNYATALGYGQANHANFDKFWNGTVFHMVGKDILRFHSIYWPILLMMLDLPMPDRLIAH
HHHHHHHHCCCCCCCCCHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYE
CEEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHCEECHH
LANDLGNLLNRTVAMINKYFDGTVPAYVDNGTAFDADLSQLIDAQLADYHKHMEAVDYPR
HHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEAIWTIIARTNKYIDETAPWVLAKEDGDKAQLASVMAHLAASLRVVAHVIQPFMMETS
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAIMAQLGLAPVSDLSTLALADFPANTKVVAKGTPIFPRLDMEAEIDYIKAQMGDSSAIS
HHHHHHHCCCCCCCHHHHHEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHC
QEKEWVPEEVALKSEKDVITFETFDAVEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQ
HHHHCCCHHHHCCCCCCEEEEEECCCEEEEHHHHHHHHHCCCCHHEEEEEECCCCCCHHH
ILSGIAKFYPNEQELVGKKLQIVANLKPRKMMKQYISQGMILSAEHGDQLTVLTVDPSVP
HHHHHHHHCCCHHHHHCCHHHEEECCCHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCC
NGSIIG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]