Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 94971952

GI number: 94971952

Start: 565666

End: 566430

Strand: Direct

Name: xthA [H]

Synonym: Dgeo_2484

Alternate gene names: 94971952

Gene position: 565666-566430 (Clockwise)

Preceding gene: 94971953

Following gene: 94971951

Centisome position: 98.53

GC content: 67.32

Gene sequence:

>765_bases
ATGAAACTCGCCACCTGGAACATCAACTCGCTCAATGTCCGTCTGCCCCAGGTGCTGGAAACGCACCGCCCGGACGTGCT
GGCCCTTCAGGAGACGAAGGTGGTCGATGAGCGCTTTCCCCTCGAACCCCTGGAGGCCCTAGGCTACGCGGCCGCCTTCG
CCGGGCAGAAGACGTACAACAGCGTCGATCTTCTCGCACGCGCGCCGCTGGAGGCCGTGCAGATGGGCATCCCCGGTCTG
GAAGACGAGCAGCGGAGGGTGCTGGTCGCGACGGTGGGGGGCGTGCGCGTCGTCTGCCTCTACGTTCCCAACGGGCAGGC
GCCCCACTCGGAGAAGTACCACTACAAGCTGGCGTGGCTGGACGCGGCCCACGCGTGGTTGCGGGACGAACTGGCGGCGC
ACGAGCGGCTGGCGGTGCTGGGGGACTTCAACGTGGCACCCGAAGACCGCGATGTCCACAACCCCAAACGCTGGGCCGGA
CAGGTGCTGGTCAGCGGGCCGGAACGTGAGGCCTTCCGGGGCCTGCTGGATGTGGGCTTTCAGGATGCCTTCCGCCTGTT
CGAGGAGCCACCCGGCGCCTTGAGCTGGTGGAACTACGGTGCACTGGCTTTCCCACGGAACTGGGGGCTGCGGATCGACC
ATGTCCTCGTTTCCCCGGCGCTGGCACAGGTCTGCCAGGCCTGCTGGGTGGACCGCGAGCCGCGTGCCCACGAACGCCCG
TCCGATCATGCCCCAGTGGTGGCAGAGTTCGGCAGCCTGCTGTAA

Upstream 100 bases:

>100_bases
TGGCTGAAAACCCAGGGCGACCGCCTGTCCACAGGCTGAAGGATTTGCCTGTGGGCTGTGGGGTCTGTTCTGAAGCCCGC
CGTGTTCCACTGGGAGCGTC

Downstream 100 bases:

>100_bases
ATAGCGGTGCGTTTTGAATTTGGGGTGTTTCTTCTCCTGAAGGGCGCGCCTCCTTGAGCGTGACTGACAACTTGGACGGG
AACGCGGAAGCGGAAAGGCG

Product: exodeoxyribonuclease III (xth)

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKLATWNINSLNVRLPQVLETHRPDVLALQETKVVDERFPLEPLEALGYAAAFAGQKTYNSVDLLARAPLEAVQMGIPGL
EDEQRRVLVATVGGVRVVCLYVPNGQAPHSEKYHYKLAWLDAAHAWLRDELAAHERLAVLGDFNVAPEDRDVHNPKRWAG
QVLVSGPEREAFRGLLDVGFQDAFRLFEEPPGALSWWNYGALAFPRNWGLRIDHVLVSPALAQVCQACWVDREPRAHERP
SDHAPVVAEFGSLL

Sequences:

>Translated_254_residues
MKLATWNINSLNVRLPQVLETHRPDVLALQETKVVDERFPLEPLEALGYAAAFAGQKTYNSVDLLARAPLEAVQMGIPGL
EDEQRRVLVATVGGVRVVCLYVPNGQAPHSEKYHYKLAWLDAAHAWLRDELAAHERLAVLGDFNVAPEDRDVHNPKRWAG
QVLVSGPEREAFRGLLDVGFQDAFRLFEEPPGALSWWNYGALAFPRNWGLRIDHVLVSPALAQVCQACWVDREPRAHERP
SDHAPVVAEFGSLL
>Mature_254_residues
MKLATWNINSLNVRLPQVLETHRPDVLALQETKVVDERFPLEPLEALGYAAAFAGQKTYNSVDLLARAPLEAVQMGIPGL
EDEQRRVLVATVGGVRVVCLYVPNGQAPHSEKYHYKLAWLDAAHAWLRDELAAHERLAVLGDFNVAPEDRDVHNPKRWAG
QVLVSGPEREAFRGLLDVGFQDAFRLFEEPPGALSWWNYGALAFPRNWGLRIDHVLVSPALAQVCQACWVDREPRAHERP
SDHAPVVAEFGSLL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=31.7829457364341, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=31.7829457364341, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=31.7829457364341, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=38.9513108614232, Blast_Score=162, Evalue=3e-41,
Organism=Drosophila melanogaster, GI221330655, Length=263, Percent_Identity=28.8973384030418, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI17136678, Length=263, Percent_Identity=28.8973384030418, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28403; Mature: 28403

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATWNINSLNVRLPQVLETHRPDVLALQETKVVDERFPLEPLEALGYAAAFAGQKTYN
CEEEEECCCCEEEECHHHHHHCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SVDLLARAPLEAVQMGIPGLEDEQRRVLVATVGGVRVVCLYVPNGQAPHSEKYHYKLAWL
HHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEEH
DAAHAWLRDELAAHERLAVLGDFNVAPEDRDVHNPKRWAGQVLVSGPEREAFRGLLDVGF
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHCEEEECCCCHHHHHHHHHCCH
QDAFRLFEEPPGALSWWNYGALAFPRNWGLRIDHVLVSPALAQVCQACWVDREPRAHERP
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SDHAPVVAEFGSLL
CCCCCHHHHHHCCC
>Mature Secondary Structure
MKLATWNINSLNVRLPQVLETHRPDVLALQETKVVDERFPLEPLEALGYAAAFAGQKTYN
CEEEEECCCCEEEECHHHHHHCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
SVDLLARAPLEAVQMGIPGLEDEQRRVLVATVGGVRVVCLYVPNGQAPHSEKYHYKLAWL
HHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEEH
DAAHAWLRDELAAHERLAVLGDFNVAPEDRDVHNPKRWAGQVLVSGPEREAFRGLLDVGF
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHCEEEECCCCHHHHHHHHHCCH
QDAFRLFEEPPGALSWWNYGALAFPRNWGLRIDHVLVSPALAQVCQACWVDREPRAHERP
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SDHAPVVAEFGSLL
CCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]