Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 94314840

GI number: 94314840

Start: 123120

End: 123953

Strand: Direct

Name: cobB [H]

Synonym: Rmet_5921

Alternate gene names: 94314840

Gene position: 123120-123953 (Clockwise)

Preceding gene: 94314839

Following gene: 94314841

Centisome position: 4.77

GC content: 67.51

Gene sequence:

>834_bases
GTGAACCATTCCGCCGCGCTTCAGGACTTCGTTGAACGGCATCGGCGCCTGTTCGTCCTGACCGGCGCCGGCATCAGCAC
GGACTCCGGCATTCCGGGCTATCGCGACGAACAAGGCCGCTGGCAACGCTCGGCGCCGATGACCATCAGCGCGTTCATGA
GCGGTCACGCGGCACGGCAGCGCTACTGGGCGCGCAGCATGGTCGGTTGGCCGGTGGCGGCGGGGGCACACCCGAATGTC
AGCCATCGCGTGGTGGCGCGGCTTGGCGATGCCGGGCGCGTGTCGGCGCTGGTTACCCAGAACGTGGACGGACTGCACCA
GCAGGCGGGCAGCCGCGATGTCGTCGAGTTGCACGGCAGCATCAGGCAGGTCGTATGCCTGTCGTGCGCCACGCGCTACC
CGCGCGCGGAGCTGCAGCACTGGCTCTGGCAACACAATCCCGATTTTCGTGACATGACGGCATTGCCCGCCGCGGACGGC
GACGCGCATCTGGAGTCGCCGTTGTTCGACAATTTCGCGGTGCCGGTTTGCGAGCGATGCGAGGGCGTGCTCAAGCCAGA
TGTGGTGTTTTTTGGCGAGTCGGTGCCGCGCGAGCGCGTCGATACCGGCCGGGCGGCACTCGCCAGCTCCGATGCCGTGC
TGGTGGTCGGGTCGTCGCTGACGGTGTTTTCGGGCTATCGCTTCTGCCTGTGGGCGAACGAGCTAGGTTTGCCGATCGCC
GCGCTGAACCTTGGCACCACGCGCGCCGATCCGCTGCTGACACTCAAGGTATCCGCGCCGATCGGGCCCACACTGTCTGC
GCTGGCGAGCGGGATCGGGCTGGCCGGCGATTGA

Upstream 100 bases:

>100_bases
TCTACAACGACGAGATGGAGCGCTATCGCCAGGAACTGCGGGCATGGGAGGCGCGCCAGGCCGCTCGCCAGGCCGCTCGC
GATAACGCCGCAACGCATTC

Downstream 100 bases:

>100_bases
CTACCGACTGACCACCGATTGACCACGGCGGCGCCCGCCGCTAGGATCGACGCATTGCCCGATATCTTCGGGTGCCCCCC
AACCAGTCATCTCATGACCC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNHSAALQDFVERHRRLFVLTGAGISTDSGIPGYRDEQGRWQRSAPMTISAFMSGHAARQRYWARSMVGWPVAAGAHPNV
SHRVVARLGDAGRVSALVTQNVDGLHQQAGSRDVVELHGSIRQVVCLSCATRYPRAELQHWLWQHNPDFRDMTALPAADG
DAHLESPLFDNFAVPVCERCEGVLKPDVVFFGESVPRERVDTGRAALASSDAVLVVGSSLTVFSGYRFCLWANELGLPIA
ALNLGTTRADPLLTLKVSAPIGPTLSALASGIGLAGD

Sequences:

>Translated_277_residues
MNHSAALQDFVERHRRLFVLTGAGISTDSGIPGYRDEQGRWQRSAPMTISAFMSGHAARQRYWARSMVGWPVAAGAHPNV
SHRVVARLGDAGRVSALVTQNVDGLHQQAGSRDVVELHGSIRQVVCLSCATRYPRAELQHWLWQHNPDFRDMTALPAADG
DAHLESPLFDNFAVPVCERCEGVLKPDVVFFGESVPRERVDTGRAALASSDAVLVVGSSLTVFSGYRFCLWANELGLPIA
ALNLGTTRADPLLTLKVSAPIGPTLSALASGIGLAGD
>Mature_277_residues
MNHSAALQDFVERHRRLFVLTGAGISTDSGIPGYRDEQGRWQRSAPMTISAFMSGHAARQRYWARSMVGWPVAAGAHPNV
SHRVVARLGDAGRVSALVTQNVDGLHQQAGSRDVVELHGSIRQVVCLSCATRYPRAELQHWLWQHNPDFRDMTALPAADG
DAHLESPLFDNFAVPVCERCEGVLKPDVVFFGESVPRERVDTGRAALASSDAVLVVGSSLTVFSGYRFCLWANELGLPIA
ALNLGTTRADPLLTLKVSAPIGPTLSALASGIGLAGD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=262, Percent_Identity=46.1832061068702, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI6912664, Length=275, Percent_Identity=29.4545454545455, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI300795542, Length=267, Percent_Identity=28.4644194756554, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI13787215, Length=259, Percent_Identity=29.7297297297297, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI13775602, Length=222, Percent_Identity=29.7297297297297, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI300797705, Length=220, Percent_Identity=29.5454545454545, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI13775600, Length=222, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI308199517, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71990482, Length=259, Percent_Identity=40.1544401544402, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71990487, Length=261, Percent_Identity=39.8467432950192, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17567771, Length=259, Percent_Identity=37.8378378378378, Blast_Score=184, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17541892, Length=219, Percent_Identity=30.5936073059361, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17505510, Length=263, Percent_Identity=25.4752851711027, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571445, Length=265, Percent_Identity=48.3018867924528, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI28571443, Length=217, Percent_Identity=48.3870967741936, Blast_Score=197, Evalue=5e-51,
Organism=Drosophila melanogaster, GI28571441, Length=217, Percent_Identity=48.3870967741936, Blast_Score=197, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29831; Mature: 29831

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSAALQDFVERHRRLFVLTGAGISTDSGIPGYRDEQGRWQRSAPMTISAFMSGHAARQ
CCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHH
RYWARSMVGWPVAAGAHPNVSHRVVARLGDAGRVSALVTQNVDGLHQQAGSRDVVELHGS
HHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCEEHHHHCCCHHHHHHCCCCCHHHHHHH
IRQVVCLSCATRYPRAELQHWLWQHNPDFRDMTALPAADGDAHLESPLFDNFAVPVCERC
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCHHHHC
EGVLKPDVVFFGESVPRERVDTGRAALASSDAVLVVGSSLTVFSGYRFCLWANELGLPIA
CCCCCCCEEEECCCCCHHHHHCCHHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCEEE
ALNLGTTRADPLLTLKVSAPIGPTLSALASGIGLAGD
EEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNHSAALQDFVERHRRLFVLTGAGISTDSGIPGYRDEQGRWQRSAPMTISAFMSGHAARQ
CCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHH
RYWARSMVGWPVAAGAHPNVSHRVVARLGDAGRVSALVTQNVDGLHQQAGSRDVVELHGS
HHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCEEHHHHCCCHHHHHHCCCCCHHHHHHH
IRQVVCLSCATRYPRAELQHWLWQHNPDFRDMTALPAADGDAHLESPLFDNFAVPVCERC
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCHHHHC
EGVLKPDVVFFGESVPRERVDTGRAALASSDAVLVVGSSLTVFSGYRFCLWANELGLPIA
CCCCCCCEEEECCCCCHHHHHCCHHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCEEE
ALNLGTTRADPLLTLKVSAPIGPTLSALASGIGLAGD
EEECCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA